scholarly journals Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Vurmila Venggadasamy ◽  
Teng Hern Loh ◽  
Jodi Woan Fei Law ◽  
Hooi-Leng Ser ◽  
Vengadesh Letchumanan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Foodborne Pathogens ◽  
Antibiotic Susceptibility ◽  
Vibrio Parahaemolyticus ◽  
Species Level ◽  
Potential Health ◽  
Potential Health Risk ◽  
Plasmid Profiles ◽  
Toxr Gene

Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, antibiotic profiles, and plasmid profiles from 77 different kinds of shellfish samples collected from wet markets and supermarkets in Selangor, Malaysia. High densities of Vibrio species ( > 5 log CFU/g) were found in 14/16 groups of shellfish. Among 77 presumptive V. parahaemolyticus isolates, 43 (55.8%) were positive for the toxR gene, confirming the identity of the isolates at the species level. However, none of the V. parahaemolyticus isolates harboured the virulence tdh and trh genes. The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. Our results indicate the V. parahaemolyticus isolates are continuously exposed to various antibiotics in the environments, thus consuming the seafood carries a potential health risk to consumers. The antibiotic resistance conferred by the species necessitates an immediate plan to approach the usage of antibiotics differently. 

scholarly journals Association of plasmid typing to biotyping and antibiotyping in the characterization of outbreaks by Acinetobacter baumannii

1999 ◽  
Vol 42 (1) ◽  
Author(s):  
Maria Cristina Bronharo Tognim ◽  
Luiz Carlos Jabur Gaziri ◽  
Marilda Carlos Vidotto ◽  
Marcia Regina Perugini
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
University Hospital ◽  
Plasmid Profiles ◽  
Typing Methods

During an outbreak at an University Hospital, from April to September, in 1994, sixteen strains of Acinetobacter baumannii were isolated from patients and one strain from an enteral solution. We afterwards analyzed the outbreak by means of plasmid typing, antibiotic resistance typing and biotyping. Two main plasmid profiles were identified. Twelve strains belonged to biotype 2, and five to biotype 19. Susceptibility to amikacin and to carbenicillin allowed classification of the strains into two groups. The results show that association of those three typing methods allowed the differentiation of what was at first considered as a single outbreak into two apparently unrelated outbreaks.

Escherichia coli Strains Isolated from Retail Meat Products: Evaluation of Biofilm Formation Ability, Antibiotic Resistance, and Phylogenetic Group Analysis

2020 ◽  
Vol 83 (2) ◽  
pp. 233-240
Author(s):  
ELENA BARILLI ◽  
ALICE VISMARRA ◽  
VIVIANA FRASCOLLA ◽  
MARTINA REGA ◽  
CRISTINA BACCI
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Health Risk ◽  
Phylogenetic Group ◽  
Animal Origin ◽  
Potential Health ◽  
Potential Health Risk ◽  
E Coli ◽  
Retail Meat

ABSTRACT Escherichia coli is a ubiquitous organism capable of forming a biofilm. This is an important virulence factor and is critical in certain diseases and in the development of antibiotic resistance, which is increased by biofilm synthesis. In the present study, the potential health risk associated with handling and consumption of foods of animal origin contaminated with E. coli–producing biofilm was evaluated. We analyzed the ability of 182 E. coli strains isolated from pork, poultry, and beef, purchased in three different supermarkets in the area of the “Italian Food Valley” (Parma, northern Italy), to form biofilms. Positive strains were also tested for the presence of 12 biofilm-associated genes. Moreover, the 182 E. coli were characterized for antibiotic resistance, presence of multidrug resistance, extended-spectrum β-lactamase strains, and phylogenetic diversity through PCR. Twenty-five percent of the isolates produced biofilm. The majority showed weak adherence, five were moderate, and three were strong producers. E. coli with a strong adherence capability (three of three) harbored eight biofilm-associated genes, while weak and moderate producers harbored only five (frequencies ranging from 80 to 100%). Multidrug resistance was observed in 20 biofilm-producing E. coli, and 15 of these belonged to phylogenetic group D. Among nonbiofilm producers, the percentage of strains belonging to phylogenetic groups B2 and D was approximately 40%, highlighting a potential health risk for consumers and people handling contaminated products. The present study underlines the importance of monitoring the prevalence and characteristics of E. coli contaminating retail meat in relation to the potential virulence highlighted here. HIGHLIGHTS

Prevalence, Antibiotic Resistance, and Molecular Characterization of Salmonella Serovars in Retail Meat Products

2011 ◽  
Vol 74 (1) ◽  
pp. 161-166 ◽  
Author(s):  
JI-YEON HYEON ◽  
JUNG-WHAN CHON ◽  
IN-GYUN HWANG ◽  
HYO-SUN KWAK ◽  
MOO-SANG KIM ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Foodborne Pathogens ◽  
Molecular Subtypes ◽  
Meat Products ◽  
Chicken Meat ◽  
Source Tracking ◽  
Traditional Markets ◽  
Retail Meats ◽  
Retail Meat

The prevalence of Salmonella was determined in chicken meat (n = 26), beef (n = 49), and pork (n = 56) collected from wholesale markets, retail stores, and traditional markets in Seoul, South Korea, in 2009. Antibiotic resistance was assessed, and the molecular subtypes of Salmonella isolates were ascertained using an automated repetitive sequence–based PCR (rep-PCR) system (DiversiLab). A total of 18 Salmonella strains were isolated from 17 of 131 samples: 16 strains from each of 16 samples and 2 strains from the same pork sample. The prevalence of Salmonella from the retail meats was 2.0% in beef, 8.9% in pork, and 42.3% in chicken meat. Among 10 different serotypes, Salmonella enterica Panama was recovered from a beef sample, and Salmonella London and Salmonella Montevideo were the predominant serotypes from pork and chicken meat, respectively. The highest antibiotic resistance observed was to erythromycin (100%) followed by streptomycin (22.2%) and tetracycline and chloramphenicol (16.7%). Of the 18 isolates, 5 (27.8%) were resistant to two or more antibiotics, and 1 isolate from chicken meat was resistant to eight antibiotics, including cephalosporins. Differentiation between all of the Salmonella isolates except between Salmonella Montevideo and Salmonella London was successfully performed with the automated rep-PCR system, indicating that it can be added to the toolbox for source tracking of foodborne pathogens associated with outbreaks.

scholarly journals Antibiotic susceptibility, plasmid isolation and curing of some foodborne pathogens

2016 ◽  
Vol 4 (2) ◽  
pp. 321
Author(s):  
Ebakota Daniel ◽  
Osarueme Osazee ◽  
Frances Olisaka ◽  
Jocelyn Aibangbee ◽  
Panmwa GALAU ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Foodborne Pathogens ◽  
Antibiotic Susceptibility ◽  
Food Production ◽  
Sodium Dodecyl ◽  
Antibiotic Resistance Genes ◽  
Enterobacter Aerogenes ◽  
Relative Sensitivity ◽  
Foodborne Bacteria ◽  
Plasmid Isolation

The indiscriminate use of antibiotics by individuals as well as in food production has been tagged one of the major reasons for the spread of antibiotic resistance in pathogens. Thus, there is a concern that foodborne bacteria may act as reservoirs of antibiotic resistance genes when distributed in large doses with food. This study aimed at determining the antibiotic susceptibility, plasmid isolation and curing of foodborne bacteria isolated from ready to eat (RTE) foods and salads in eating centers at the Benson Idahosa University, Benin City. Isolates were Enterobacter aerogenes, Escherichia coli, Staphylococcus aureus, Bacillus spp., Micrococcus sp. and Salmonella sp with S. aureus occurring most frequently. Total resistance to cefuroxime and augmentin as well as considerable resistance to ceftazidime and cefixime were observed in all isolates in antimicrobial susceptibility tests were done on Mueller-Hinton agar. Relative sensitivity to gentamicin, ofloxacin, nitrofurantoin and ciprofloxacin were observed. Plasmid profiling indicated that all isolates possess plasmids ranging from 100 bp to 1 kbp. Plasmid curing using sodium dodecyl sulfate (SDS) improved the sensitivity of isolates to antibiotics they were previously sensitive to but most isolates remained resistance to ceftazidime, cefuroxime, cefixime, and augmentin. This study shows that foodborne bacteria can possess and possibly transfer persistent antibiotic resistance plasmids thus calling for more caution in the use of antibiotics in food production and reduced antibiotics abuse. Further research is currently ongoing to cure the isolates of all plasmids and to elucidate how these plasmids are being transferred.

scholarly journals Isolation and Characterization of Listeria monocytogenes Isolates from Ready-To-Eat Foods in Florida

2006 ◽  
Vol 72 (7) ◽  
pp. 5073-5076 ◽  
Author(s):  
Yuelian Shen ◽  
Yan Liu ◽  
Yifan Zhang ◽  
Jennifer Cripe ◽  
William Conway ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antibiotic Susceptibility ◽  
Food Samples ◽  
Multiple Antibiotic Resistance ◽  
Isolation And Characterization ◽  
Lineage 2 ◽  
Conventional Methods ◽  
Specific Bacteriophage

ABSTRACT Of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for Listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. Seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an L. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods.

scholarly journals Identification of Vibrio parahaemolyticus Strains at the Species Level by PCR Targeted to the toxR Gene

1999 ◽  
Vol 37 (4) ◽  
pp. 1173-1177 ◽  
Author(s):  
Yung Bu Kim ◽  
Jun Okuda ◽  
Chiho Matsumoto ◽  
Naoki Takahashi ◽  
Satoru Hashimoto ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Vibrio Parahaemolyticus ◽  
Sequence Variation ◽  
Vibrio Alginolyticus ◽  
Species Level ◽  
Specific Detection ◽  
Colony Hybridization ◽  
Hybridization Test ◽  
Toxr Gene ◽  
Positive Results

The DNA colony hybridization test with the polynucleotide probe forVibrio parahaemolyticus toxR gene was performed. All 373 strains of V. parahaemolyticus gave positive results, and the strains belonging to four other Vibrio species including Vibrio alginolyticus gave weakly positive results, suggesting that toxR sequence variation may reflect the phylogenetic relationships of Vibrio species. We then established a toxR-targeted PCR protocol for the specific detection of V. parahaemolyticus.

scholarly journals Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254081
Author(s):  
Xiaoying Wu ◽  
Bei Wu ◽  
Yue Li ◽  
Xiue Jin ◽  
Xiliang Wang
Keyword(s):  
Adverse Effect ◽  
Antibiotic Resistance ◽  
Safety Assessment ◽  
Animal Experiments ◽  
Opportunistic Pathogens ◽  
Phenotypic Analysis ◽  
Fermented Sausage ◽  
Potential Health ◽  
Potential Health Risk ◽  
Subjective Synonym

Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E. sanguinicola. In this study, Enterococcus thailandicus TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that cylA, gelE, esp, agg, ace, efaAfm, efaAfs, ptsD genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated β-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that E. thailandicus TC1 may be associated with a potential health risk.

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