scholarly journals ANTIFUNGAL ACTIVITY AND PHYTOCHEMICAL PROFILE OF CHLOROFORM SOLUBLE FRACTION OF DATURA METEL FRUIT

2013 ◽  
Vol 7 (16) ◽  
pp. 1605-1612 ◽  
Author(s):  
Rinez Asma ◽  
Daami Remadi Mejda ◽  
Ladhari Afef ◽  
Omezzine Faten ◽  
Rinez Imen ◽  
...  

Author(s):  
Jithu U. Krishnan ◽  
Meera George ◽  
Ajesh G.

Objective: Medicinal plants are a source of great economic value all over the world and are on the front dusk of traditional medicine. Daturametel L, is one among the foresaid and this plant can be explored further as per its diversity of uses and on the basis of wide range of chemical compounds reported to be present in various parts of the plant. In the present investigation, Phyto-chemistry of D. metel has been analysed along with its antibacterial property.Methods: The selected plant leaves were collected and powdered. The bioactive compounds were extracted by using different solvents. Various phytochemical tests for alkaloids, flavonoids, saponins, steroids, terpenoids, tannins, glycosides and amino acids were done accordingly with standard protocol of testing. The study also extends to view the anti-bacterial potential by determining inhibition of growth using agar well diffusion method.Results: Phytochemical analysis reflected the presence of compounds like alkaloids, flavonoids, saponins, tannins, glycosides, and amino acids. It was noted that steroids and tannins are absent. Antimicrobial activity proves extract of ethanolis the most promising against the pathogens from the rest screened.Conclusion: Thearticle addresses, the undocumented knowledge regarding the phytochemical profile of D. metel which demand further exploration widely to serve Humanity. Antimicrobial potential of ethanol extract are very promising to deliver it as a pharmaceutical.


Author(s):  
A. Hannah Hepsibah ◽  
G. Jeya Jothi

<p><strong>Objective: </strong>Plants contain different classes of phytochemicals which have different levels of solubility in solvents, based on their polarity. Hence, this study aims to compare the effects of various solvents on the phytochemical profile and biological potential of <em>Ormocarpum cochinchinense</em>. The present study is the first to evaluate the antifungal activity of the plant.<strong></strong></p><p><strong>Methods: </strong>The sequential extraction was carried out using two sets of solvent systems namely hexane, ethyl acetate, ethanol and chloroform, acetone, methanol. The extracts were subjected to standard phytochemical analysis, antimicrobial activity by disc diffusion method against eight bacteria and six fungi and antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay.</p><p><strong>Results: </strong>Varied range of phytochemicals was found in the extracts. Acetone extract was rich in phenolic compounds, whereas terpenoids were extracted only in methanol. Acetone extracts showed highest antibacterial activity with a maximum zone of 14.5 mm against <em>Shigella flexineri</em> whereas ethyl acetate extract showed the best antifungal activity with the highest zone of inhibition of 12 mm against <em>Trichophyton menta agrophytes.</em> Hexane and chloroform extracts did not show any antimicrobial activity. For DPPH assay, the ethanol extracts showed the highest percentage inhibition of 92.87%. <strong></strong></p><p><strong>Conclusion: </strong>The present investigation on the plant <em>O. cochinchinense</em> has proved that the selection of solvent for extraction should be based on the target compounds and their bioactivity and concludes that acetone was the best for the extraction of antibacterial compounds and ethyl acetate for antifungal compounds whereas, ethanol was the best for extracting antioxidant compounds in <em>O. cochinchinense.</em></p>


Mycobiology ◽  
2001 ◽  
Vol 29 (2) ◽  
pp. 96-99 ◽  
Author(s):  
U. P. Singh ◽  
Om Prakash ◽  
A. B. Ray

Holzforschung ◽  
1999 ◽  
Vol 53 (5) ◽  
pp. 487-490 ◽  
Author(s):  
Shang-Tzen Chang ◽  
Sheng-Yang Wang ◽  
Chi-Lin Wu ◽  
Yu-Chang Su ◽  
Yueh-Hsiung Kuo

Summary This study was to isolate and identify the antifungal compounds in the ethyl acetate soluble fraction of the methanol extractives of Taiwania (Taiwania cryptomerioides Hayata) heartwood and to examine their antifungal activity. Five compounds were obtained by open column chromatography and HPLC and based upon the results from Mass, 1H-NMR, and 13C-NMR analyses. Their structures were identified, namely ferruginol, helioxanthin, savinin, taiwanin C, and hinokiol. According to the results of antifungal test, the order of antifungal index of these compounds for Coriolus versicolor (L. ex Fr.) Quel. was ferruginol > taiwanin C > savinin > hinokiol. For Laetiporus sulphureus (B. ex Fr.) Bond. it was taiwanin C > savinin > ferruginol > hinokiol.


2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Saud Bawazeer ◽  
Abdur Rauf ◽  
Sami Bawazeer

This study deals with α-glucosidase and β-secretase inhibitory screening of extract/fractions and isolated daturaolone (1), namely, 3-oxo-6-β-hydroxy-β-amyrin (daturaolone) from chloroform fraction of Datura metel L. Among entire fractions, the chloroform soluble fraction showed excellent activity against α-glucosidase with % inhibition 90.8 with IC50160.2±1.85 μg and daturaolone (1) with 98.7% inhibition with IC50840.4±1.74 μM, respectively. Similarly, extract and daturaolone (1) also exhibited significant activity against the β-secretase enzyme (BACE1) with % activities 88.27 and 95.19 and with IC50 values 304.21±2.98 μg and 260.70±1.87 μM, respectively, as compared to the standard inhibitor (Ans670, Sta671, Val672)-amyloid-β/A4 precursor protein 770 fragments 662-675) with % activity 94.21 and IC50 value 289.24±1.60 μM. This finding encourages and opens a new window for further detail phytochemical investigation on D. metel in order to isolate novel compounds with promising enzyme inhibitory potential.


2020 ◽  
Vol 11 (2) ◽  
pp. 2650-2656
Author(s):  
Varsha A Bhalerao ◽  
Ashok M Chavan

About 90% of the world food crops are being produced by using seeds. Healthy seeds are important for production of healthy crop and these seeds are also responsible for disease transmission. It happens either in field or in post-harvest storage condition. Due to these seed borne fungi, seed get deteriorated which may cause a great economic loss as well as several types of abnormalities occur in seeds. Major storage grain fungi contaminated with mycotoxin, mycotoxins contaminants of fungal origin occurring worldwide and characterized by its acute and chronic toxic effects on human health. In the present attempt were made to study the antifungal activity of leaf extract against mycotoxin producing fungi. Fungi isolated from stored seed grains like  cereals, pulses, infected oil seeds were collected from from Marathwada region of Maharashtra, India. Antifungal activity of leaf extract of Calotropis procera, Azadirachta indica, Ocimum sanctum, Withania somnifera and Datura metel were tested against Alternaria alternata, Aspergillus flavus, Aspergillus ochraceus, Fusarium gramineorum and Penicillium citrinum by using 96-well plate method. Leaf extract showed remarkable activity against tested fungi at different concentrations as Minimum Inhibitory Concentration. The leaf extract of Calotropis procera and Azadirachta indica, have good potential antifungal properties against different mycotoxin producing fungi.


Author(s):  
D. Umaiyambigai ◽  
K. Saravanakumar ◽  
G. Adaikala Raj

The present study was aimed to antifungal activity and phytoconstituents of leaves from thePsydrax dicoccosGaertn.The antifungal activity of methanol extract from theP. dicoccosagainstCandida albicans,C. krusei,C. guilliermondii,C. parapsilosis,C. tropicalis, C.glabrata, four dermatophytesviz.,Trichophyton rubrum,T. mentagrophytes,Microsporum gypseumandEpidermophyton flocossum.The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FT-IR) and GasChromatography-Mass Spectroscopic (GC-MS) analysis. The mean zones of inhibition produced by the tested extract in disc diffusion assays against fungal strains were ranged from 7.3 to 15.5 mm. The MIC values were between 125 and 500 µg/ml while, the MFC values were between 250 and 1000 µg/ml. The highest mean zones of inhibition (15.5±0.6 mm) was observed with methanol extract ofP. dicoccosagainstC. albicans. The GC-MS analysis ofP. dicoccosleaves showed the presence of cinnamic acid, 2H-1-Benzopyran-2-one,5,7-dimethoxy,(Z)6,(Z)9-Pentadecadien-1-ol, Benzofuran and n-Hexadecanoic acid as major compounds. Finally it can be concluded that the antifungal activity may be present in cinnamic acid, 2H-1-Benzopyran-2-one, 5, 7-, (Z) 6,(Z)9-Pentadecadien-1-ol, n-Hexadecanoic acid from the methanol extract from theP. dicoccosis highly valuable in medicinal usage and have fewer side effects.


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