scholarly journals Phytochemical profile and antifungal activity of stems and leaves methanol extract from the Juncus maritimus Linn. Juncaceae family against some dermatophytes fungi

2020 ◽  
Author(s):  
Sarab Fadhil AlAmery ◽  
Neepal lmtair AlGaraawi
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Juncus Maritimus ◽  
Phytochemical Profile ◽  
Stems And Leaves

Phytochemical Profile and Antifungal Activity of Leaves Methanol Extract from the Psydrax dicoccos (Gaertn) Teys. & Binn. Rubiaceae Family

2017 ◽  
Vol 7 ◽  
pp. 53-61 ◽  
Author(s):  
D. Umaiyambigai ◽  
K. Saravanakumar ◽  
G. Adaikala Raj
Keyword(s):  
Antifungal Activity ◽  
Cinnamic Acid ◽  
Methanol Extract ◽  
Microsporum Gypseum ◽  
Hexadecanoic Acid ◽  
Ms Analysis ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
The Mean ◽  
Phytochemical Profile

The present study was aimed to antifungal activity and phytoconstituents of leaves from thePsydrax dicoccosGaertn.The antifungal activity of methanol extract from theP. dicoccosagainstCandida albicans,C. krusei,C. guilliermondii,C. parapsilosis,C. tropicalis, C.glabrata, four dermatophytesviz.,Trichophyton rubrum,T. mentagrophytes,Microsporum gypseumandEpidermophyton flocossum.The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FT-IR) and GasChromatography-Mass Spectroscopic (GC-MS) analysis. The mean zones of inhibition produced by the tested extract in disc diffusion assays against fungal strains were ranged from 7.3 to 15.5 mm. The MIC values were between 125 and 500 µg/ml while, the MFC values were between 250 and 1000 µg/ml. The highest mean zones of inhibition (15.5±0.6 mm) was observed with methanol extract ofP. dicoccosagainstC. albicans. The GC-MS analysis ofP. dicoccosleaves showed the presence of cinnamic acid, 2H-1-Benzopyran-2-one,5,7-dimethoxy,(Z)6,(Z)9-Pentadecadien-1-ol, Benzofuran and n-Hexadecanoic acid as major compounds. Finally it can be concluded that the antifungal activity may be present in cinnamic acid, 2H-1-Benzopyran-2-one, 5, 7-, (Z) 6,(Z)9-Pentadecadien-1-ol, n-Hexadecanoic acid from the methanol extract from theP. dicoccosis highly valuable in medicinal usage and have fewer side effects.

scholarly journals Antimicrobial and antioxidant potentials of non-cytotoxic extracts of corticolous lichens sampled in Armenia

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Razmik Sargsyan ◽  
Arsen Gasparyan ◽  
Gohar Tadevosyan ◽  
Hovik Panosyan
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Bacterial Strains ◽  
Evernia Prunastri ◽  
Wide Range ◽  
Antibacterial And Antifungal ◽  
Acetone Extracts

AbstractDue to wide range of secondary metabolites, lichens were used from antiquity as sources of colorants, perfumes and medicaments. This research focuses on exploring the antioxidant, antimicrobial and cytotoxic activities of methanol, ethanol, acetone extracts and aqueous infusions of corticolous lichens sampled from Armenia. Methanol, ethanol and acetone extracts from all tested lichens were active against Gram-positive bacterial strains. The most effective solvent to retrieve antimicrobial compounds was methanol. Aqueous infusions of tested lichens didn’t show any significant antibacterial and antifungal activity. The highest antimicrobial activity was observed for methanol extract of Ramalina sinensis. The minimum inhibitory concentration of methanol extract of Ramalina sinensis were 0.9–1.8 mg mL− 1. Pseudevernia furfuracea demonstrated antifungal activity (Ø 12 mm). Methanol extract of Parmelia sulcata demonstrated largest 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity (71 %). The cytotoxicity was measured on human HeLa (cervical carcinoma) cell lines using microculture tetrazolium test assay. The IC50 values estimated for methanol extracts of Peltigera praetextata, Evernia prunastri, Ramalina sinensis and Ramalina farinacea species in HeLa cell line were within 1.8–2.8 mg mL− 1 and considered as non-cytotoxic. Obtained results suggest that studied lichens can be prospective in biotechnologies as alternative sources of antimicrobial and antioxidant substances.

scholarly journals POTENSI EKSTRAK DAUN CENDANA (Santalum album L.) SEBAGAI SENYAWA PENGHAMBAT JAMUR Candida albicans

Jurnal Kimia ◽  
2019 ◽  
pp. 159
Author(s):  
K. Swandiyasa ◽  
N. M. Puspawati ◽  
I. A. R. A. Asih
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Santalum Album ◽  
Web Database ◽  
Nail Diseases ◽  
Leaf Powder ◽  
Santalum Album L ◽  
Crude Methanol Extract

Diseases caused by C. albicans (C. albicans) such as mouth, skin and nail  diseases are still commonly found in Indonesia. This study aimed to examine antifungal activity of n-hexane, chloroform, and n-butanol extracts of Santalum album (S.album) leaves in inhibiting the growth of C. albicans and to determine the minimum inhibitory concentration of the most active extract as well as to identify their active compounds. Antifungal activity testing was carried out using disc diffusion method and identification of active compound was performed using LC-MS /MS. Extraction of 1 kg sandalwood leaf powder with methanol yielded 86.80 gram of crude methanol extract. Partiiton 40 gram of the crude methanol extract with n-hexane, chloroform, and n-buthanol gave 12.60, 6.20 and 1.20 g of extracts respectively. The antifungal activity test results revealed that n-hexane extract was the most active in inhibiting the growth of C.albicans with inhibitory diameter of 13 mm as compared to chloroform 9 mm and n-buthanol 8 mm. The active n-hexane extract which is active as an antifungal is then made various variations to obtain a minimum inhibitory. The n-hexane extract, at concentrations of 95, 90 and 85% inhibited the growth of C.albican with inhibitory diameter of 12.04, 15.8, and 13 mm respectively. While at the concentarion of 80 % showed minimum inhibitory diameter of 9.32 mm. Based on analysis mass spectra of two peaks of LC-MS / MS chromatogram with MassLynx V4.1 programe and Chemspider web database suggested the presence of benzofurazan and picolylamine compounds which may contribute to the antifungal activityof n-hexane extract of S.album. Keywords: Sandalwood (Santalum album L.), antifungal, C. albicans, n-Hexane, LC-MS/MS

scholarly journals THE PHYTOCHEMICAL SCREENING HEXANE AND METHANOL EXTRACT OF SINYO NAKAL (DURANTA REPENS)

2020 ◽  
pp. 196-200
Author(s):  
PURWANTININGSIH SUGITA ◽  
RIZKI AMILIA ◽  
BUDI ARIFIN ◽  
DYAH UTAMI CAHYANING RAHAYU ◽  
HANHAN DIANHAR
Keyword(s):  
Fatty Acids ◽  
Developing Countries ◽  
Linoleic Acid ◽  
Vitamin E ◽  
Methanol Extract ◽  
Stem Bark ◽  
Unsaponifiable Fraction ◽  
Phytochemical Screening ◽  
Phytochemical Profile ◽  
Based Medicine

Objective: Phytochemicals are naturally present in every part of plants range from leaves, stem bark, and fruits to roots. The use of plant-based medicine is popular among individuals and communities in developing countries. This study evaluated that the phytochemical profile of Duranta repens fruits was collected from Jombang, East Java, Indonesia. Methods: Air-dried D. repens fruits were macerated by n-hexane and methanol, a separate flask. n-Hexane extract, then underwent saponification to remove fat. Then, the unsaponifiable of n-hexane and methanol extract was tested through phytochemical screening, respectively. Results: The methanol extract showed the presence of alkaloids, flavonoids, tannins, and steroids, but the unsaponifiable fraction only contained steroids. The unsaponifiable fraction was found to be a mixture of hydrocarbons ranging from C13-C20, fatty acids (palmitic and linoleic acid), squalene, and Vitamin E. Conclusion: The phytochemical screening of the plant plays an important role for pharmaceutical studies especially discovering new potential drugs for the treatment of various diseases.

Additional Studies on the Antifungal Activity of a Methanol Extract of Ipomoea Carnea Subsp. Fistulosa and Octadecyl P-Coumarates

2016 ◽  
Vol 6 (3) ◽  
pp. 148-151 ◽  
Author(s):  
Eugene Sebastian John Nidiry ◽  
Girija Ganeshan ◽  
Ankanahalli Narayanashetty Lokesha ◽  
Nanjundagowda Ramachandran
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Ipomoea Carnea

scholarly journals Anti –phytopathogenic Activities of Cladophora glomerata extract against plant fungi

2014 ◽  
Vol 11 (2) ◽  
pp. 883-887 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Antimicrobial Agents ◽  
Methanol Extract ◽  
Cladophora Glomerata ◽  
Damping Off ◽  
Natural Antimicrobial ◽  
Plant Fungi ◽  
Pharmaceutical Industries ◽  
Crude Methanol Extract

The antiphytopathogenic effects of the crude methanol extract of Cladophora glomerata (Lin.) Kützing (Cladophoraceae) which isolated from Al Rashidiya region at north of Baghdad was investigated against two type of plant fungi (Pythium altimum and Rhizoctonia solani) which causes damping off disease where isolated from covered cucumber field in Al-Alyosifia region. Hot methanol extract showed antifungal activity against the two species of fungi in different concentrations(10,25,50 mg/ml) of extract to Cladophora glomerata as percentage inhibition (51.63 ,72.8 ,83.71) and (56.18 ,77.41 ,100)comparing with Pythium altimum and Rhizoctonia solani respectively .primary detection of active compounds showed that macroalgae (Cladophora glomerata) containing flavonoids,alkaloids, phenols and tannins. Our findings suggest the possibility of using the Cladophora glomerata as a novel source of natural antimicrobial agents for pharmaceutical industries.

scholarly journals Antimicrobial and Antioxidant Potentials of Non-cytotoxic Extracts of Corticolous Lichens Sampled in Armenia

2021 ◽  
Author(s):  
Razmik Sargsyan ◽  
Arsen Gasparyan ◽  
Gohar Tadevosyan ◽  
Hovik Panosyan
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Evernia Prunastri ◽  
Wide Range ◽  
Alternative Sources ◽  
Antibacterial And Antifungal ◽  
Acetone Extracts

Abstract Due to wide range of secondary metabolites, lichens were used from antiquity as sources of colorants, perfumes and medicaments. This research focuses on exploring the antioxidant, antimicrobial and cytotoxic activities of methanol, ethanol, acetone extracts and aqueous infusions of corticolous lichens sampled from Armenia. Methanol, ethanol and acetone extracts from all tested lichens were active against Gram-positive bacterial strains. The most effective solvent to retrieve antimicrobial compounds was methanol. Aqueous infusions of tested lichens didn’t show any significant antibacterial and antifungal activity. The highest antimicrobial activity was observed for methanol extract of Ramalina sinensis. The minimum inhibitory concentration of methanol extract of Ramalina sinensis were 0.9 to 1.8 mg mL-1. Pseudevernia furfuracea demonstrated antifungal activity (Ø 12 mm). Methanol extract of Parmelia sulcata demonstrated largest DPPH radical scavenging activity (71%). The cytotoxicity was measured on human HeLa (cervical carcinoma) cell lines using microculture tetrazolium test assay. The IC50 values estimated for methanol extracts of Peltigera praetextata, Evernia prunastri, R. sinensis and R. farinacea species in HeLa cell line were within 1.8-2.8 mg mL-1 and considered as non-cytotoxic. Obtained results suggest that studied lichens can be prospective in biotechnologies as alternative sources of antimicrobial and antioxidant substances.

scholarly journals The Effects of Tormentic Acid and Extracts from Callistemon citrinus on Candida albicans and Candida tropicalis Growth and Inhibition of Ergosterol Biosynthesis in Candida albicans

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Chido Bvumbi ◽  
Godloves Fru Chi ◽  
Marc Y. Stevens ◽  
Molly Mombeshora ◽  
Stanley Mukanganyama
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Candida Tropicalis ◽  
Methanol Extract ◽  
Fungal Infections ◽  
Water Extract ◽  
Antifungal Drugs ◽  
Ergosterol Content ◽  
Broth Microdilution Method ◽  
Tormentic Acid

Candida albicans and Candida tropicalis are the leading causes of human fungal infections worldwide. There is an increase in resistance of Candida pathogens to existing antifungal drugs leading to a need to find new sources of antifungal agents. Tormentic acid has been isolated from different plants including Callistemon citrinus and has been found to possess antimicrobial properties, including antifungal activity. The study aimed to determine the effects of tormentic and extracts from C. citrinus on C. albicans and C. tropicalis and a possible mode of action. The extracts and tormentic acid were screened for antifungal activity using the broth microdilution method. The growth of both species was inhibited by the extracts, and C. albicans was more susceptible to the extract compared to C. tropicalis. The growth of C. albicans was inhibited by 80% at 100 μg/ml of both the DCM: methanol extract and the ethanol: water extract. Tormentic acid reduced the growth of C. albicans by 72% at 100 μg/ml. The effects of the extracts and tormentic acid on ergosterol content in C. albicans were determined using a UV/Vis scanning spectrophotometer. At concentrations of tormentic acid of 25 μg/ml, 50 μg/ml, 100 μg/ml, and 200 μg/ml, the content of ergosterol was decreased by 22%, 36%, 48%, and 78%, respectively. Similarly, the DCM: methanol extract at 100 μg/ml and 200 μg/ml decreased the content by 78% and 88%, respectively. A dose-dependent decrease in ergosterol content was observed in cells exposed to miconazole with a 25 μg/ml concentration causing a 100% decrease in ergosterol content. Therefore, tormentic acid inhibits the synthesis of ergosterol in C. albicans. Modifications of the structure of tormentic acid to increase its antifungal potency may be explored in further studies.

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