Phytochemical Profile and Antifungal Activity of Leaves Methanol Extract from the Psydrax dicoccos (Gaertn) Teys. & Binn. Rubiaceae Family

2017 ◽  
Vol 7 ◽  
pp. 53-61 ◽  
Author(s):  
D. Umaiyambigai ◽  
K. Saravanakumar ◽  
G. Adaikala Raj
Keyword(s):  
Antifungal Activity ◽  
Cinnamic Acid ◽  
Methanol Extract ◽  
Microsporum Gypseum ◽  
Hexadecanoic Acid ◽  
Ms Analysis ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
The Mean ◽  
Phytochemical Profile

The present study was aimed to antifungal activity and phytoconstituents of leaves from thePsydrax dicoccosGaertn.The antifungal activity of methanol extract from theP. dicoccosagainstCandida albicans,C. krusei,C. guilliermondii,C. parapsilosis,C. tropicalis, C.glabrata, four dermatophytesviz.,Trichophyton rubrum,T. mentagrophytes,Microsporum gypseumandEpidermophyton flocossum.The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FT-IR) and GasChromatography-Mass Spectroscopic (GC-MS) analysis. The mean zones of inhibition produced by the tested extract in disc diffusion assays against fungal strains were ranged from 7.3 to 15.5 mm. The MIC values were between 125 and 500 µg/ml while, the MFC values were between 250 and 1000 µg/ml. The highest mean zones of inhibition (15.5±0.6 mm) was observed with methanol extract ofP. dicoccosagainstC. albicans. The GC-MS analysis ofP. dicoccosleaves showed the presence of cinnamic acid, 2H-1-Benzopyran-2-one,5,7-dimethoxy,(Z)6,(Z)9-Pentadecadien-1-ol, Benzofuran and n-Hexadecanoic acid as major compounds. Finally it can be concluded that the antifungal activity may be present in cinnamic acid, 2H-1-Benzopyran-2-one, 5, 7-, (Z) 6,(Z)9-Pentadecadien-1-ol, n-Hexadecanoic acid from the methanol extract from theP. dicoccosis highly valuable in medicinal usage and have fewer side effects.

Effect of Synthesized Ag Nanoparticles by Using the Different Amounts of Polyvinylpyrrolidone for Ag-Natural Rubber Hybrid Sheets and their Antibacterial Properties

2017 ◽  
Vol 751 ◽  
pp. 270-276 ◽  
Author(s):  
Warot Prasanseang ◽  
Chaval Sriwong ◽  
Kittisak Choojun
Keyword(s):  
Natural Rubber ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Rubber Matrix ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
The Mean

Ag-natural rubber (Ag-NR) hybrid sheets were successfully prepared with a very simple and low cost method. In this method, silver nanoparticles (AgNPs) were firstly synthesized by a rapid and green microwave-assisted using polyvinylpyrroridone (PVP) media. The effect of PVP weight ratios towards the size of AgNPs was also investigated. Then, Ag-NR hybrid sheet samples were prepared by latex mixing-casting method using concentrated natural rubber (NR) latex with the synthesized AgNPs colloids. The characteristic absorption, particles sizes and shapes of the obtained AgNPs were examined through UV-vis, TEM and SAED. Also, the prepared Ag-NR sheet samples were characterized using XRD, FT-IR, SEM and EDS techniques. It was found that the particles sizes of all the synthesized AgNPs had spherical-like shape, and the mean sizes were increased from 29.7 to 90.4 nm upon increasing PVP contents. EDS results showed the AgNPs were well-dispersed and impregnated into the rubber matrix. Moreover, the antibacterial properties of the prepared Ag-NR sheets were tested by agar disk-diffusion method with Gram-positive and Gram-negative bacteria as Staphylococcus aureus(S. aureus) and Escherichia coli(E. coli), respectively. The results showed that the hybrid sheets exhibited excellent antibacterial properties against these bacteria, in which the zones of inhibition were also dependent on the synthesized AgNPs by utilizing the different amounts of PVP.

scholarly journals Phytochemical Analysis and Antifungal Activity of Ficus Sycomorus L. (Moraceae) Extracts on some Dermatophytic Fungi

2021 ◽  
Vol 6 (1) ◽  
pp. 11-17
Author(s):  
Umar Lawal ◽  
◽  
L. Mansir ◽  
M. G. Sani
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Fungal Infections ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Microsporum Canis ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Dermatophytic Fungi ◽  
Ficus Sycomorus

Ficus sycomorus L is a large, semi-deciduous spreading savannah tree that belongs to the family (Moraceae) is used by many tribes in the treatments of skin infections. The aim of this study was to carry out phytochemical analysis and antifungal evaluation of F. sycomorus L. stem and leaf Methanol extract on some dermatophytic fungi. The stem and leaf crude extracts were obtained after drying using maceration and percolation with methanol as the solvent. The extract was subjected to qualitative phytochemical screening as well as GC-MS analysis for the presence of bioactive constituents. The antifungal inhibitory effect of the crude stem and leaf extracts was assessed by agar well diffusion methods. The phytochemical screening revealed that the leaves and stem-bark contained secondary metabolites such as tannins, flavonoids, alkaloids, saponins, reducing sugar and steroid. The GC-MS assay showed that the most prevailing compound in the methanolic stem-bark extract was 2, 9, Diazabicyclo (4, 4, 0) decane-3,10 dione, while in the methanolic leaves extract was acetic acid. The anti-dermatophytic assay of the extracts against Microsporum canis and Tricophyton tonsurans showed that the extracts inhibited the fungi with zones of inhibitions ranging from 6.33±0.47 to 24.33±0.47mm with the leaf extract exhibiting higher inhibition zones than the stem extract (p˂0.01). The study shows that both the stem and leaf methanol extract of Ficus sycomorus L possesses antifungal activity and should be considered as a potential antifungal agent in search of newer agents to address dermatophytic fungal infections Key words: Ficus sycomorus L, Phytochemical/GC-MS Analysis, Antifungal activity

scholarly journals GC-MS and FT-IR Profiling of leaves methanol extract from the Pleiospermium alatum (Wall. ex Wt. & Arn) Swingle Rutaceae family

2016 ◽  
Vol 5 (5) ◽  
pp. 201-204
Author(s):  
K. Saravanakumar ◽  
◽  
Adaikala Raj ◽  
D. Umaiyambigai ◽  
◽  
...  
Keyword(s):  
Methanol Extract ◽  
Chemical Compounds ◽  
Hexadecenoic Acid ◽  
Bioactive Constituents ◽  
Ms Analysis ◽  
Ft Ir ◽  
Ir Analysis ◽  
Major Chemical ◽  
Potential Use ◽  
Protein Enzyme

The present study was aimed to analysis of bioactive constituents of leaves from the Pleiospermium alatum (Rutaceae). The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FTIR) and Gas chromatography- mass spectroscopic (GC-MS) analysis. GC-MS analysis of plant extract was performed using a Perkin-Elmer GC Clarus 500 system and Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) and IR spectrum was recorded in spectrophotometer (Thermo Scientific NICOLET-iS5). FT-IR analysis of peak values with various functional compounds such as amizone, alcohol, phenol, alkanes, protein, enzyme, alkanes, isopropyl. GC-MS analysis of compounds with totally, thirty compounds major chemical compounds were identified, such as 5- Thio-D-glucose, 5-Allylsulfanyl-1-(4-methoxy-phenyl)-1H-tetrazole, E)-10-Heptadecen-8-ynoicacid methyl ester and Z-11-Hexadecenoic acid. The present results concluded that the phytochemicals was observed in methanol extract which revealed that the P. alatum is potential use in different fields namely medical and pharmaceuticals and highly valuable in medicinal usage for the treatment of various human aliments.

scholarly journals Anticandidal and Antidermatophytic Activities of Caulerpa Species from the Gulf of Mannar Coast, Mandapam Tamilnadu India

2017 ◽  
Vol 7 ◽  
pp. 30-40
Author(s):  
G. Adaikala Raj ◽  
S. Jegan ◽  
S. Krishnamoorthy ◽  
M. Chandrasekaran ◽  
V. Venkatesalu
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Gulf Of Mannar ◽  
Microsporum Gypseum ◽  
Disc Diffusion ◽  
Agar Disc ◽  
Zones Of Inhibition

The present investigation was carried out to evaluate the antifungal activity of hexane, chloroform, ethyl acetate, acetone and methanol extracts ofCaulerpa chemnitzia, (Epser) J.V. LamououxC. racemosa(Forsk),andC. scalpelliformis(R.Br.) Weber-van-Bosse, (Chlorophyceae) againstCandida albicans,C. krusei,C. guilliermondii,C. parapsilosis,C. tropicalis,C.glabrata, four dermatophytesviz.,Trichophyton rubrum,T. mentagrophytes,Microsporum gypseumandEpidermophyton flocossum. The antifungal activity was evaluated by agar disc diffusion method, determination of MIC and MFC. The mean zones of inhibition produced by the extracts in disc diffusion assay against the tested fungal strains were ranged from 7.1 to 15.1 mm. The lowest MIC (250 µg/ml) and MFC (500 µg/ml) values were observed in the ethyl acetate extract ofC. racemosaagainstC.parapsilosis,C. albicans,C. krusei,C. glabrata,C. guilliermondii, T. rubrum, M. gypseumandT. mentagrophytes.The finding suggests that ethyl acetate extract ofC. racemosashowed the higher antifungal activity againstC.parapsilosisandC. albicans.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals Antimicrobial and antioxidant potentials of non-cytotoxic extracts of corticolous lichens sampled in Armenia

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Razmik Sargsyan ◽  
Arsen Gasparyan ◽  
Gohar Tadevosyan ◽  
Hovik Panosyan
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Bacterial Strains ◽  
Evernia Prunastri ◽  
Wide Range ◽  
Antibacterial And Antifungal ◽  
Acetone Extracts

AbstractDue to wide range of secondary metabolites, lichens were used from antiquity as sources of colorants, perfumes and medicaments. This research focuses on exploring the antioxidant, antimicrobial and cytotoxic activities of methanol, ethanol, acetone extracts and aqueous infusions of corticolous lichens sampled from Armenia. Methanol, ethanol and acetone extracts from all tested lichens were active against Gram-positive bacterial strains. The most effective solvent to retrieve antimicrobial compounds was methanol. Aqueous infusions of tested lichens didn’t show any significant antibacterial and antifungal activity. The highest antimicrobial activity was observed for methanol extract of Ramalina sinensis. The minimum inhibitory concentration of methanol extract of Ramalina sinensis were 0.9–1.8 mg mL− 1. Pseudevernia furfuracea demonstrated antifungal activity (Ø 12 mm). Methanol extract of Parmelia sulcata demonstrated largest 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity (71 %). The cytotoxicity was measured on human HeLa (cervical carcinoma) cell lines using microculture tetrazolium test assay. The IC50 values estimated for methanol extracts of Peltigera praetextata, Evernia prunastri, Ramalina sinensis and Ramalina farinacea species in HeLa cell line were within 1.8–2.8 mg mL− 1 and considered as non-cytotoxic. Obtained results suggest that studied lichens can be prospective in biotechnologies as alternative sources of antimicrobial and antioxidant substances.

scholarly journals Characterization and Catalytic Behaviour of Co3(PO4)2–AlPO4 Catalysts

1998 ◽  
Vol 16 (4) ◽  
pp. 285-293 ◽  
Author(s):  
M.R. Mostafa ◽  
F.Sh. Ahmed
Keyword(s):  
Pore Radius ◽  
Surface Acidity ◽  
Total Pore Volume ◽  
Textural Properties ◽  
Acid Sites ◽  
Adsorption Of Nitrogen ◽  
Amorphous Structures ◽  
Ft Ir ◽  
Catalytic Behaviour ◽  
The Mean

Co3(PO4)2, AlPO4 and the binary system Co3(PO4)2-AlPO4 with different compositions were prepared by the coprecipitation method. The structural properties of these samples were determined using XRD, DTA and FT-IR techniques. The textural properties were determined from the adsorption of nitrogen at 77 K. The surface acidity was measured by a calorimetric titration method. The samples were tested as catalysts in the dehydration of ethanol and isopropanol using a pulse microcatalytic technique. The data obtained from XRD and FT-IR indicate the amorphous structures of the prepared catalysts. An increase in Co3(PO4)2 content led to a decrease in the surface area and in the total pore volume and an increase in the mean pore radius. The surface acidity of the catalyst depends on the chemical composition; the surface acidity increased with an increase in the AlPO4 content. The dehydration temperature and the distribution of acid sites are important parameters in determining the selectivity and activity of the catalyst.

scholarly journals Organ-Specific Phytochemical Profiling and Antioxidant Analysis of Parthenium hysterophorus L.

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Javed Ahmad ◽  
Rita Bagheri ◽  
Humayra Bashir ◽  
M. Affan Baig ◽  
Asma Al-Huqail ◽  
...  
Keyword(s):  
Antioxidant Potential ◽  
Parthenium Hysterophorus ◽  
High Threshold ◽  
Hexadecanoic Acid ◽  
Biotic And Abiotic Stresses ◽  
Organ Specific ◽  
Global Concern ◽  
Phytochemical Profile ◽  
Stem Leaf

Parthenium hysterophorus is a weed of global concern with high threshold of tolerance against most of biotic and abiotic stresses. Phytochemical profile and in vitro antioxidant analysis may help in understanding its tolerance to stresses. Root, stem, leaf, phyllary, and receptacle (including disc and ray florets) were chemotyped employing GC tof-MS and assessed for antioxidant activity by DPPH, FRAP, HRSA, and TAC assays. Phytochemicals identified were terpenes, fatty acids, hydrocarbons, phytosterols, and compounds of miscellaneous chemical nature. Organ-specific maximum concentration of metabolite was β-vatirenene (root), hexadecanoic acid methylester (stem), aristolene epoxide (leaf), hexadecanoic acid methylester (phyllary), and hexadecanoic acid methylester (receptacle). Identified metabolites could be associated with stress tolerance mechanisms, basic metabolism, and allelopathy, etc. Root extracts showed highest antioxidant potential followed by receptacle. It can be concluded that diverse and unique phytochemical profile and great antioxidant potential make P. hysterophorus stress-tolerant, hence a weed of global habitat.

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