scholarly journals EFEK PENYIMPANAN SECARA KRIOPRESERVASI PADA JENIS DAN LAMA PERENDAMAN KRIOPROTEKTAN DENGAN PERBEDAAN KONDISI BIJI TERHADAP VIABILITAS BENIH MANGGIS (Garcinia mangostana L.)

2014 ◽  
Vol 5 (2) ◽  
Author(s):  
Dede Suhendra

Effect Storage In Criopreservasi On Type And Old South Crioprotektan Different Conditions Of Seeds Against Viability of Mangosteen Seeds (Garcinia mangostana L.). The aim of the study was to determine the type, duration of soaking and the different conditions of mangosteen seeds and their interaction with the viability of mangosteen seeds (Garcinia mangostana L.). This research was carried out at the Laboratory of Applied Sciences in Seed Technology of Labuhanbatu College of Agricultural Sciences (STIPER), starting March to July 2018, using factorial randomized block design with 3 treatment factors namely cryoprotectant type, cryoprotectant immersion and mangosteen fruit flesh. Parameters observed were normal sprouts (%), abnormal sprouts (%), dead seeds (%). The results showed that the highest application of the parameters was the use of non-fruit flesh with cryoprotectant PVS2 and 180 minutes soaking time. The results of the research were used to develop mangosteen seed storage for the needs of the community, especially mangosteen farmers in Indonesia. Keywords : crioprotektan, criopreservasi, mangosteen seeds

1970 ◽  
Vol 6 (1) ◽  
pp. 49-57
Author(s):  
Dede Suhendra

Effect Storage In Criopreservasi On Type And Old South Crioprotektan Different Conditions Of Seeds Against Growth of Mangosteen Seeds (Garcinia mangostana L). The aim of the study was to determine the type, duration of soaking and the different conditions of mangosteen seeds and their interaction with the growth of mangosteen seeds (Garcinia mangostana L). This research was carried out at the Laboratory of Applied Sciences in Seed Technology of Labuhanbatu College of Agricultural Sciences (STIPER), starting March to July 2018, using factorial randomized block design with 3 treatment factors namely cryoprotectant type, cryoprotectant immersion and mangosteen fruit flesh. Parameters observed were number of shoots (fruit), shoot height (cm), root length (cm), and sprout dry weight (g). The results showed that the highest application of the parameters was the use of non-fruit flesh with cryoprotectant PVS2 and 180 minutes soaking time. The results of the research were used to develop mangosteen seed storage for the needs of the community, especially mangosteen farmers in Indonesia.


1970 ◽  
Vol 3 (3) ◽  
pp. 235-248
Author(s):  
Dede Suhendra ◽  
T. Chairun Nisa ◽  
Diana Sofia Hanafiah

The purpose of this study is to determine the best number of seeds cuttings, gibberellin concentration and immersion time for the germination of mangosteen seeds. This research conducted at Laboratory of Seed Technology Faculty Agriculture, University of Sumatera Utara, Medan from December to March 2016. Experimental design used a factorial randomized block design with three factors, seed cutting, gibberellin concentrations and immersion time. Parameters measured were membrane leakage (μmhos), normal seedling (%), abnormal seedling (%), died seeds (%), germination rate (days), vigor Index (%). The results show that intact mangosteen seeds or without cutting, application of gibberellin at a concentration of 75 ppm and 24 hours time immersion are the best treatments to mangosteen seed germination.


2021 ◽  
Vol 4 (2) ◽  
pp. 122-129
Author(s):  
Enda Sabda Gentri Sembiring ◽  
Julaili Irni ◽  
Rama Riana Sitinjak ◽  
Bayu Pratomo

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors


2017 ◽  
Vol 3 (2) ◽  
pp. 9
Author(s):  
Asep Samsul Mustopa

The experiment was carried out at Plant Biotechnology laboratorium and experimentalfield Faculty of Agriculture University of Winaya Mukti Tanjungsari Sumedang which the altitude is about 850 meters above sea level, from March 2008 until May 2008.Theobjective of this experiment was to studying the interaction effect of giberellat acidconcentration and and soaking time on seed viability, seed vigor, growth and yield ofJatropha curcas in nursery.Design used was Randomized Block Design (RBD) withfactorial pattern, treatment consisted of two factors and two replications. The first factorwas giberellat acid (GA3) concentration (K) which consisted of 4 levels namely : k1=250 ppm, k2= 500 ppm, k3= 750 ppm and k4= 1000 ppm. The second factor soakingtime on GA34 (M) which consisted of 4 levels namely : m1= 6 hour, m2= 18 hour dan m = 24 hour.The results of these experiment showed there was aninteraction between giberellat acid concentration and and soaking time on high of plant20 day after planting (DAP), 40 DAP, 60 DAP, number of leaves 20 DAP, 40 DAP, 60DAP, length of root, number of root, dry wight of root and dry wight of shoot. 


2019 ◽  
Vol 7 (1) ◽  
pp. 81
Author(s):  
Komang Adi Darmawan ◽  
Ni Made Wartini ◽  
Luh Putu Wrasiati

Marigold powder can be used as natural orange dyes extract of marigold powder contain ?-carotene. The aim of this research was to investigate the effect of sodium metabisulfite (Na2S2O5) concentration and soaking time on the characteristics of marigold flower powder and determine the best treatment of the concentration of sodium metabisulphite (Na2S2O5) and soaking time to produce powdered marigold flowers. This research used factorial randomized block design with 2 factor experiments. The first factor is the concentration of sodium metabisulfite (Na2S2O5) which consists of 3 levels: 2000, 2500, and 3000 ppm. The second factor is the soaking time which consists of 3 levels: 10, 20, and 30 minutes. The results of research showed that the treatment of sodium metabisulfite (Na2S2O5) concentration take effect on water content, total carotenoid levels, brightness (L*), yellowish level (b*), and sulfite residue levels, but no effect to the level of redness (a*). The treatment of soaking time take effect on the levels of sulfite residues but no effect on water content, total carotenoid levels, brightness level (L*), yellowish level (b*), and redness level (a*). Soaking treatment in sodium metabisulfite (Na2S2O5) solution with a concentration of 2500 ppm and soaking time of 10 minutes, is the best treatment to produce marigold flower powder with the characteristics of water content 11,40%, total carotenoid levels of 18.91%, brightness level (L*) 44.19, redness level (a*) 63.80, yellowish level (b*) 56.86, and sulfite residue levels of 182.40 ppm. Keywords : Tagetes erecta L, sodium metabisulfite, carotenoid, color.


2018 ◽  
Vol 3 (2) ◽  
pp. 351-356
Author(s):  
Weny Weny ◽  
Rasdiansyah Rasdiansyah ◽  
Novia Mehra Erfiza

Abstrak. Sampai saat ini belum ada penelitian lebih lanjut mengenai pemanfaatan hasil samping dari produk asam sunti. Tujuan penelitian ini untuk melihat pengaruh perendaman ikan di dalam air asam sunti dan penyimpanannya pada suhu ruang terhadap karakteristik ikan. Penelitian ini menggunakan Rancangan Acak Kelompok Faktorial dengan 2 faktor yaitu faktor waktu perendaman (W), yang terdiri dari 3 taraf (W1 : 30 detik, W2 : 5 menit, W3 : 10 menit) dan lama penyimpanan (S), yang terdiri dari 2 taraf (S1 : 0 jam, S2 : 2 jam). Hasil penelitian menunjukkan bahwa karakteristik air asam sunti pada uji pH memiliki rataan yaitu 1,27, total asam 49% dan total bakteri asam laktat  5,1×104 CFU/gram. Nilai pH ikan setelah direndam air asam sunti 5,22-5,65 dengan rataan 5,43 dan nilai protein terlarut berkisar antara 18,42-18,74% dengan rataan 18,74%.Dari penelitian didapatkan bahwa waktu perendaman (W) berpengaruh nyata (P≤0,05) terhadap nilai protein terlarut dari ikan namun berpengaruh tidak nyata (P0,05) terhadap nilai pH ikan, uji deskriptif aroma dan tekstur ikan. Lama penyimpanan (S) berpengaruh sangat nyata (P≤0,01) terhadap nilai pH ikan setelah perendaman namun berpengaruh tidak nyata (P0,05) terhadap uji deskriptif aroma dan tekstur ikan. Interaksi waktu perendaman dengan lama penyimpanan berpengaruh nyata (P≤0,05) terhadap nilai pH ikan namun berpengaruh tidak nyata terhadap uji deskriptif aroma dan tekstur ikan.Waktu perendaman ikan di dalam air asam sunti selama 10 menit dapat menghambat kenaikan pH ikan selama penyimpanan sampai 2 jam. Semakin lama perendaman ikan di dalam air asam sunti maka semakin tinggi protein terlarut dari ikan.  Abstrack. Therefore, this study was conducted to see the effect of soaking the fish in asam sunti water and its storage at room temperature on the characteristics of the fish. This study uses a randomized block design factorial with 2 factors: soaking time (W), consisting of 3 levels (W1: 30 seconds, W2: 5 minutes, W3: 10 minutes) and storage time (S), consisting of 2 levels ( S1: 0 hours, S2: 2 hours). The results showed pHof asam sunti water is 1.27,  total acid is 49% and lactic acid bacteria has  of  5.1 × 104 CFU / gram and the soaked fish has pH values ranged from 5.22 to 5.65 with the average of 5.43 and soluble protein values ranged from 18.42 to 18.74% with the average 18.74%.The results indicated that the soaking time (W) has effect significantly to the value of soluble protein (P≤0,05) of fish but iteffect not significantly (P 0.05) on pH value of fish, descriptive test the of aroma and texture of the fish. Storage time (S) effect significantly (P≤0,01) to pH value of fish after soaking but it effectnot significantly (P 0.05) to the descriptive test of  aroma and texture of the fish. Interaction withsoaking time and storage (WxS) effect significantly (P≤0,05) to pH value of fish but it effect to not significantly the descriptive test of aroma and texture of the fish.Soaking time the fish in water for 10 minutes sunti can inhibit increasing in the pH of the fish during storage up to 2 hours. The longer soaking time of the fish in the asam sunti water, the higher sunti acid soluble proteins from value.


2020 ◽  
Vol 3 (2) ◽  
pp. 48-60
Author(s):  
Billy Andreas Putra ◽  
Tengku Boumedine Hamid Zulkifli ◽  
Yunida Berliana ◽  
Octanina Sari Sijabat

Introduction: The purpose of this study was to determine the dosage of inorganic fertilizers, soaking time of the inoculation of the legume bacteria strains which were suitable for the growth and yield of peanut in tidal lowland. Materials and Methods: This research was conducted at tidal lowland in Sei Ular Village, Secanggang sub-District, Langkat District, North Sumatra from September to December 2019. The research used randomized block design in factorial. The first factor is the dose of inorganic fertilizers consisting of P0 = un-fertilizer (control), P1= urea 50 kg.ha-1 + TSP 180 kg.ha-1 + KCl 50 kg.ha-1, P2= urea 75 kg.ha-1 + TSP 205 kg.ha-1 + KCl 75 kg.ha-1, P3= urea 100 kg.ha-1 + TSP 230 kg.ha-1 + KCl 100 kg.ha-1. The second factor was the soaking time of the Bradyrhizobium japonicum bacterial strain consisting of T0= un-soaking time, T1= soaking time for 3 minutes, T2= soaking time for 6 minutes, and T3= soaking time for 9 minutes. Parameters were analyzed using ANOVA and BNJ analysis 5%.· Results: The application of urea 50 kg.ha-1 + TSP 180 kg.ha-1 + KCl 50 kg.ha-1 significantly increased the plant height, number of leaves, and root volume and showed the highest number of active nodules, dry pods.plot-1 and seed weight.plot-1 compared to other doses. The seed soaking time with the Bradyrhizobium japonicum strain and their interactions showed insignificant differences in all parameters of peanut plants in tidal lowland.


Author(s):  
Silvia Polhaupessy ◽  
Hermalina Sinay

Background: The aim of this research was want to know the effect of GA concentration with soaking time towards seedling germination of soursop, and to know whichone of the treatment that can give a very highest quality and quantity of seedling germination of soursop. Method: This research started from  Juny until  July 2011. Factorial pattern of completely randomized block design (RAL) were used in this research, which consist of two factors. First factor where concentration of GA with four level i.e. A1= 5 PPM, A2 = 10 PPM, A3 = 15 PPM, and A4 = 20 PPM. While the second factor where soaking time which consist of three level i.e. L1= soaking for six hour, L2 = soaking for twelve hours, L3 = soaking for twenty-four hours. Each combination with three replicated. Data collected were analyzed used analysis of variance at the significant level of α 0,05 and 0,01. Result: Percentage obtained at the A3L2 treatment i.e. 100 %, plant high 16,12 cm, and root length 12,99 cm. It can be concluding that GA and soaking time treatment, can affected the seedling germination of soursop. Conclusion: Based on the results obtained it can be concluded that the use of GA concentration and different soaking time on seed soursop significant effect on percentage germination, high germination and seedling root length.


2018 ◽  
Vol 3 (2) ◽  
pp. 12-19
Author(s):  
Sarah Sakinah Umadi ◽  
Sumadi Sumadi ◽  
Denny Sobardini Sobarna

The decrease of soybean productivity was caused by low quality of seed. To improve the quality of seed, soybean seed were coated  with Trichoderma sp. and adding bokashi organic fertilizer. This research aimed at finding the best dose combination of Trichoderma sp.  and bokashi fertilizer to improve the quality of soybeans. The research was conducted in the experiment field and Laboratory of Seed Technology at Padjadjaran University in April - August 2017. The experimental design in this research was Randomized Block Design (RBD). The treatments were the combination  of four dose values of coating the seed by Trichoderma sp. (0g/100 seeds, 1g/100 seeds, 2g/100 seeds, and 3g/100 seeds) and three dose  of bokashi (0g/polybag, 300g/polybag, and 600g/polybag) and each treatment was replicated three times. The experiment result showed that all treatments on soybean seeds did not affect the germination percentange, vigor index, and conductivity value, but affected the seed quantityof 600g/polybag of bokashi and without seed coating with Trichoderma sp.  on 100 seed mass and seed weight per plant.


2020 ◽  
Vol 6 (2) ◽  
pp. 19-22
Author(s):  
Dede Suhendra ◽  
Yudi Triyanto ◽  
Siti Hartati Yusida Saragih ◽  
Zahlul Ikhsan

Rosela (Hibiscus sabdariffa L.) is a medicinal plant. Rosela has a chemical compound that can provide many benefits. Efficacy of rosella to prevent disease, treat disorders of various diseases with gossiptin anthocyanin content and gluciside hibiscin contained in it. This research was conducted at the Laboratory of College of Agricultural Sciences of Labuhan Batu University Foundation, North Sumatera Province. This research was started in March 2017. This research used Non-Factorial Randomized Block Design (RAK) with the harvesting period (P) P0: Control, P1: 1, Clock P2: 2 Hours, P3: 3 Hours P4: 4 Hours P5: 5 Hours P6: 6 Hours P: 7 Hours. The highest rate of germination was found in P7 treatment of 3.22 days. The highest normal germination percentage was found in P5 and P7 treatment of 66.67%. Than highest abnormal germination percentege was found in P3, P4 and P6 treatment of 33.33 %. Keywords : Rosela, Old Immersion


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