scholarly journals Phytochemical and Antioxidant Test of Binahong (Anredera cordifolia (Tenore) Steenis) Leaves Ethanol Extract

2021 ◽  
Vol 6 (1) ◽  
pp. 28
Author(s):  
Fensia Analda Souhoka ◽  
Imanuel Berly Delvis Kapelle ◽  
Elisabeth Sihasale
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Absorbance Measurement ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

Binahong Anredera cordifolia (Tenore) Steenis leaves contain chemical compounds that can be used as antioxidants. This study aims to examine the phytochemical and antioxidant activity of the ethanol extract of binahong leaves. Binahong leaves were extracted using the maceration method with an ethanol solvent. The phytochemical test showed that the ethanol extract of binahong leaves contained phenolic compounds, flavonoids, alkaloids, and tannins to have potential antioxidants. The antioxidant activity test was carried out using the DPPH method and absorbance measurement with a UV-Vis spectrophotometer at a wavelength of 517 nm. The results showed that the ethanol extract of binahong leaves had strong antioxidant activity, as evidenced by the IC50 value of 87.423 µg/mL.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals STUDI OPTIMASI EKSTRAKSI KANDUNGAN SENYAWA FENOLIK TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN DARI DAUN MANGGIS (Garcinia mangostana Linn.)

2019 ◽  
Vol 10 (1) ◽  
pp. 36
Author(s):  
Emil Salim ◽  
Yogi Afritunando ◽  
Nindi Antika Febriana ◽  
Mai Efdi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Garcinia Mangostana ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

This study aims to determine antioxidant activity and total phenolic content of mangosteen leaves in several different solvents. The antioxidant activity test of the extract was determined by the DPPH method and the total phenolic content was determined by the Folin-Ciocalteu method. The phytochemical test of mangosteen leaves showed the presence of phenolic compounds, saponins, triterpenoids and alkaloids. The extraction results increase with increasing water content in methanol, ethanol and acetone. The highest amount of extract was obtained using 50% acetone solvent (34.98 ± 0.17%). Extraction using 100% ethanol showed the highest total phenolic content (614.25 ± 5.58 mg GAE / g extract) and the lowest IC50 value (5.81 mg / L). The results of this study indicate that the use of 50% acetone solvent can optimize the amount of extract and 100% ethanol can optimize the content of total phenolic compounds and antioxidant activity of mangosteen leaves.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SELADA ROMAINE (Lactuca sativa var. Longifolia) DAN DAUN SELADA KERITING (Lactuca sativa var. Crispa) BESERTA IDENTIFIKASI BEBERAPA SENYAWA ANTIOKSIDAN

2019 ◽  
Vol 16 (01) ◽  
pp. 21
Author(s):  
Maria Ulfah ◽  
Ady Laksono Putro ◽  
Efa Erfiana Safitri
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Lactuca Sativa ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Romaine Lettuce ◽  
Ic50 Value ◽  
Dpph Method ◽  
C Value ◽  
Layer Chromatography

ABSTRACTFree radicals are suspected as triggers of various degenerative diseases. Antioxidants are compounds that can counteract free radicals. This study aims to determine the antioxidant activity by DPPH method and analyze the active compounds contained in romaine lettuce extract (Lactuca sativa var Longifolia) and lactuca sativa var Crishpa lettuce extract (Lactuca sativa var Crishpa). Romaine lettuce and and lactuca sativa var Crishpa was done extraction with a maceration method using 70% as ethanol solvent. The concentration of extract used was 12.5; 25; 50; 100; 200 and 400 ppm and vitamin C concentrations as comparison was 0.5; 1; 1.5; 2; 2,5 and 3 ppm tested its antioxidant activity by DPPH method by spectrophotometry visible, until IC50 value was obtained. The identification of the active compound was performed by phytochemical screening and Thin Layer Chromatography (TLC). The results showed that romaine lettuce has IC50 value of 151,1515 ppm and lactuca sativa var Crishpa has IC50 value of 183,7560 ppm and vitamin C value was 1,7005 ppm. The chemical compounds contained in romaine ethanol extract and lactuca sativa var Crishpa lettuce extract are alkaloids, phenols and flavonoids.Keywords: Antioxidant, DPPH, IC50, TLC, Romaine, Lactuca sativa var Crishpa

scholarly journals Antioxidant Activity Test for Rukam Fruit (Flacourtia rukam) Of Maseration Extract

2019 ◽  
Vol 1 (1) ◽  
pp. 14-19
Author(s):  
Inas Fadiyah ◽  
Iin Lestari ◽  
Shelly Victory
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Wild Plants ◽  
Fruit Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

Rukam fruit (Flacourtia rukam) is one of the wild plants that are widely distributed in Indonesia, especially on the island of Bangka. The results of previous studies stated that rukam fruit has a phenolic content of 40 mg GAE / 100 grams. Phenolic compounds have major benefits as antioxidants, where antioxidants have the ability to inhibit the work of free radicals. Therefore a study was conducted to assess the content of secondary metabolites and the antioxidant bioactivity of the Flacourtia rukam species using maceration method. Rukam fruit extract was obtained using the Maceration method. As for the antioxidant test using the DPPH` method. The results of the antioxidant activity test of rukam extract with ethanol solvent obtained IC50 value 236,169 µg / mL and in rukam extract with acetone solvent obtained IC50 276,443 µg / mL which showed that the antioxidant properties of this rukam were moderate.

scholarly journals Phytochemicals and GC-MS of Aquilaria beccariana van Tiegh leaves growing naturally and cultivated in North Sumatera

2021 ◽  
Vol 912 (1) ◽  
pp. 012079
Author(s):  
R Batubara ◽  
W Pulungan ◽  
T I Hanum ◽  
O Affandi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Antioxidant Potential ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method

Abstract Aquilaria beccariana van Tiegh is one of the agarwood (gaharu) species that grows naturally and cultivatived. However, the chemical and antioxidant potential of its leaves have not been studied much. This research aimed to examine the chemical compounds and the antioxidant activity of the ethanol extract of the agarwood leaves A. beccariana van Tiegh which grows naturally and cultivatived. The leaves were processed into simplicia and then extracted using 96% ethanol as solvent. Phytochemical screening agarwood leaves to determine the chemical compounds such as of alkaloids, flavonoids, glycosides, steroids/triterpenoids, saponins and tannis. The antioxidant activity test was carried out using the DPPH method to obtain the IC50 (Inhibitory Concentration) value. The compound tracing used GC-MS. The results showed that the simplicia and the extract of the leaves both natural-grown and cultivated contained secondary metabolites of alkaloids, flavonoids, steroids/triterpenoids, and tannins positively, whereas the glycosides were only found in cultivated type. The result of the antioxidant activity test showed that the ethanol extract of the leaves of A. beccariana van Tiegh both natural-grown and cultivated was categorized as very strong. The results of the identification of chemical compounds by GC-MS were identified 18 compounds in the natural-grown leaves, while 40 compounds were identified in the cultivated ones.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN MANGGA KASTURI (Mangifera casturi Kosterm.)

2021 ◽  
Vol 3 (3) ◽  
pp. 162-173
Author(s):  
Dwi Lestari ◽  
Muthia Dwi MA ◽  
Jati Pratiwi ◽  
Lidya Handoko Saputri
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

Mangga Kasturi (Mangifera casturi Kosterm.) is one of the plants in Indonesia that has potential as an antioxidant. The purpose of this study was to determine the IC50 value and antioxidant potential of the ethanol extract of mangga kasturi leaves (Mangifera casturi Kosterm.). The ethanol extract of mangga kasturi leaves (Mangifera casturi Kosterm.) was extracted using maceration with 96% ethanol as solvent. The antioxidant activity test was carried out using the DPPH (1,1-Diphenyl-2-Picrylhydrazil) method using a UV-Vis spectrophotometer and vitamin C as a comparison. The results of phytochemical screening tests on ethanol extracts showed positive secondary metabolites such as alkaloids, flavonoids, tannins, and quinones. Testing the ethanol extract of Mangga Kasturi leaves (Mangifera casturi Kosterm.) with the DPPH method showed that the ethanol extract had an antioxidant activity with an IC50 value of 83.61 ppm in the strong category of antioxidant potential.

scholarly journals Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method

2021 ◽  
Vol 7 (2) ◽  
pp. 275
Author(s):  
Dewa Ayu Puspaning Kumaradewi ◽  
Windah Anugrah Subaidah ◽  
Yayuk Andayani ◽  
Ali Al-Mokaram
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Color Test ◽  
Activity Test ◽  
Dpph Method

Research on phytochemical screening and antioxidant activity testing of the ethanol extract of buni leaves (Antidesma bunius L. Spreng) has been carried out. This study aims to identify secondary metabolite compounds contained in buni leaf plants to see the activity of the ethanol extract of buni leaves using the DPPH method. Buni leaves were extracted in 96% ethanol by maceration method, then evaporated using a rotary evaporator. The results of thick extract were screened for phytochemicals such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids using a qualitative color test method and carrying out antioxidant activity using a UV-Vis spectrophotometer with Vitamin C as a comparison. The results showed that the ethanol extract of buni leaves contained phenolic compounds, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as IC50 Vitamin C of 5.22 ppm which is classified as a very strong antioxidant, while the IC50 of buni leaves ethanol extract is lower at 61, 8 ppm which is classified as a strong antioxidant

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

Ekstraksi Protein dari Rhodophyta dan Chlorophyta Dari Perairan Pulau Pari Sebagai Alternatif Antioksidant

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Elvi Kustiyah ◽  
Bungaran Saingin ◽  
Hernowo Widodo ◽  
Viriya Piti
Keyword(s):  
Antioxidant Activity ◽  
Low Temperature ◽  
Protein Content ◽  
Green Algae ◽  
Red Algae ◽  
Marine Resources ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Lowry Method

 Indonesia has millions island and big part of Indonesia is sea that is rich in marine biological resources and has the potential to be developed and optimized. One of the abundant marine resources in Indonesia is algae. Algae are plant-like protists. Algae have several important compounds, including protein, carbohydrates, fats, minerals and other useful elements. Proteins contained in algae have the potential to be used as antioxidants. In this study, the levels of protein in red and green algae were tested by using the lowry method and testing the antioxidant activity of red and green algae extracts using the Diphenylpicrylhydrazyl (DPPH) method. Algae extraction was done by maceration, which is soaking the sample in low temperature with phosphate buffer saline (PBS) pH 7. From the extraction results it can be concluded that the red algae (Rhodophyta) has the highest protein content of 5.115 ± 0.126% and the lowest protein content in green algae (Chloropytha) as big as 1.686 ± 0.430%. And from the results of the antioxidant activity test showed that all positive algae showed antioxidant activity but the green algae (Chlorophyta) had the highest antioxidant activity of 71.5946 ± 0.01612% with IC50 value 1.6114.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

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