Simone ASHBY: Co-producers of this means of expression’: Evidence from Mozambique in support of the study of indigenizing languages

2016 ◽  
Keyword(s):  
Expression Evidence

Role of the avian retrovirus mRNA leader in expression: evidence for novel translational control

1986 ◽  
Vol 6 (2) ◽  
pp. 372-379
Author(s):  
R A Katz ◽  
B R Cullen ◽  
R Malavarca ◽  
A M Skalka
Keyword(s):  
Translational Control ◽  
Consensus Sequence ◽  
Viral Gene Expression ◽  
State Level ◽  
Viral Gene ◽  
Translational Initiation ◽  
Efficient Expression ◽  
Expression Characterization ◽  
Expression Evidence

Avian retroviral mRNAs contain a long 5' untranslated leader of approximately 380 nucleotides. The leader includes sequences required for viral replication and three AUG codons which precede the AUG codon used for translational initiation of the gag and env genes. We have used sensitive, quantitative assays of viral gene transcription and translation to analyze the role of this mRNA leader in viral gene expression. By substituting segments from related viruses, we had previously shown that the endogenous avian provirus ev-1 contained a defective leader segment (B. R. Cullen, A. M. Skalka, and G. Ju, Proc. Natl. Acad. Sci. USA 80:2946-2950, 1983). The sequence analysis presented here, followed by comparison with the nondefective ev-2 endogenous provirus segment, identified the critical changes at nucleotides 4 and 7 upstream of the initiator AUG. These differences do not alter the most conserved nucleotides within the consensus sequence which precedes eucaryotic initiation codons, but lie within a nine-nucleotide region that is otherwise highly conserved among avian retrovirus strains. Analysis of a series of deletion mutants indicated that other sequences within the leader are also required for efficient expression. Characterization of the altered transcripts demonstrated that the presence of the defective ev-1 segment or the deletion of a ca. 200-nucleotide leader segment did not affect the steady-state level or splicing efficiency of these mRNAs. Thus, we conclude that the reduced expression of these mRNAs is due to a translational deficiency. These results indicate that specific leader sequences, other than the previously identified consensus nucleotides which precede eucaryotic AUG initiator codons, can influence eucaryotic gene translation.

scholarly journals The Effects of Aphasia on the use of Evaluative Expression: Evidence from Verbal Mimicry in Narrative

2012 ◽  
Vol 61 ◽  
pp. 230-231 ◽  
Author(s):  
G. Olness ◽  
C. Brewster ◽  
R. Dutra
Keyword(s):  
Expression Evidence

scholarly journals Masculinities in Transit: The Voices of Motorcyclists

2019 ◽  
Vol 8 (2) ◽  
pp. 145
Author(s):  
Johanna Burbano-Valente ◽  
Martin Emilio Gafaro-Barrera ◽  
Angelica Paola Torres-Quintero ◽  
María Teresa Dominguez-Torres
Keyword(s):  
Interpersonal Relationships ◽  
Transition Process ◽  
Risky Behaviors ◽  
Public And Private ◽  
Social Transformations ◽  
Social Interventions ◽  
The Social ◽  
Semistructured Interviews ◽  
Expression Evidence ◽  
In Transit

This paper reports on the construction of masculinities in the narratives of 11 motorcyclists in Valledupar – Colombia. The aim was to establish the ways masculinities are expressed and recreated by motorcycle riders and the importance of motorcycles in these expressions. To do this, semistructured interviews were conducted to search for the evolution of their personal stories as men and on their relationship with motorcycles. We found that their masculinities are currently “in transit”: they vary from hegemonic manifestations to peripheral masculine ways of expression. Evidence of these transits can be grouped around four emerging categories: risk behavior, amusement settings, sexual expression and roles in public and private settings. Results show that, in some of these categories, the hegemonic patriarchal masculine logic prevails, especially through risky behaviors and motorcycle riding. However, other disruptive ways of expressing masculinities were found, motivated by the social transformations in equality policies, changes in interpersonal relationships and the participants’ own experiences. Supporting the transition process of masculinities is a large responsibility in social interventions seeking for a more equitable and fair society.

Characterization of an amphioxus paired box gene, AmphiPax2/5/8: developmental expression patterns in optic support cells, nephridium, thyroid-like structures and pharyngeal gill slits, but not in the midbrain-hindbrain boundary region

Development ◽  
1999 ◽  
Vol 126 (6) ◽  
pp. 1295-1304 ◽  
Author(s):  
Z. Kozmik ◽  
N.D. Holland ◽  
A. Kalousova ◽  
J. Paces ◽  
M. Schubert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Central Nervous System ◽  
Nervous System ◽  
Expression Patterns ◽  
Boundary Region ◽  
Developmental Expression ◽  
Developmental Gene Expression ◽  
Expression Evidence ◽  
Engrailed Genes ◽  
Otic Vesicles

On the basis of developmental gene expression, the vertebrate central nervous system comprises: a forebrain plus anterior midbrain, a midbrain-hindbrain boundary region (MHB) having organizer properties, and a rhombospinal domain. The vertebrate MHB is characterized by position, by organizer properties and by being the early site of action of Wnt1 and engrailed genes, and of genes of the Pax2/5/8 subfamily. Wada and others (Wada, H., Saiga, H., Satoh, N. and Holland, P. W. H. (1998) Development 125, 1113–1122) suggested that ascidian tunicates have a vertebrate-like MHB on the basis of ascidian Pax258 expression there. In another invertebrate chordate, amphioxus, comparable gene expression evidence for a vertebrate-like MHB is lacking. We, therefore, isolated and characterized AmphiPax2/5/8, the sole member of this subfamily in amphioxus. AmphiPax2/5/8 is initially expressed well back in the rhombospinal domain and not where a MHB would be expected. In contrast, most of the other expression domains of AmphiPax2/5/8 correspond to expression domains of vertebrate Pax2, Pax5 and Pax8 in structures that are probably homologous - support cells of the eye, nephridium, thyroid-like structures and pharyngeal gill slits; although AmphiPax2/5/8 is not transcribed in any structures that could be interpreted as homologues of vertebrate otic placodes or otic vesicles. In sum, the developmental expression of AmphiPax2/5/8 indicates that the amphioxus central nervous system lacks a MHB resembling the vertebrate isthmic region. Additional gene expression data for the developing ascidian and amphioxus nervous systems would help determine whether a MHB is a basal chordate character secondarily lost in amphioxus. The alternative is that the MHB is a vertebrate innovation.

Distinct functional contributions of 2 GABP–NRF-2 recognition sites within the context of the human TOMM70 promoter

2006 ◽  
Vol 84 (5) ◽  
pp. 813-822 ◽  
Author(s):  
José R. Blesa ◽  
José Hernández-Yago
Keyword(s):  
Chromatin Immunoprecipitation ◽  
Binding Sites ◽  
Site Directed Mutagenesis ◽  
Outer Mitochondrial Membrane ◽  
Mobility Shift ◽  
A Subunit ◽  
Expression Evidence ◽  
Electrophoretic Mobility Shift Assays

TOMM70 is a subunit of the outer mitochondrial membrane translocase that plays a major role as a receptor of hydrophobic preproteins targeted to mitochondria. We have previously reported 2 binding sites for the transcription factor GABP–NRF-2 in the promoter region of the human TOMM70 gene that are important in activating transcription. To assess the functionality and actual role of these sites, chromatin immunoprecipitation, site-directed mutagenesis, and electrophoretic mobility shift assays were carried out. We conclude that GABP–NRF-2 binds in vivo to the TOMM70 promoter, and that the 2 GABP–NRF-2 binding sites of the promoter have different functional contributions in promoting TOMM70 expression. Evidence is provided that they work in an additive manner as single sites.

scholarly journals Atlantic Salmon Type I IFN Subtypes Show Differences in Antiviral Activity and Cell-Dependent Expression: Evidence for High IFNb/IFNc–Producing Cells in Fish Lymphoid Tissues

2012 ◽  
Vol 189 (12) ◽  
pp. 5912-5923 ◽  
Author(s):  
Tina Svingerud ◽  
Terese Solstad ◽  
Baojian Sun ◽  
May Liss J. Nyrud ◽  
Øyvind Kileng ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Atlantic Salmon ◽  
Lymphoid Tissues ◽  
Type I ◽  
Type I Ifn ◽  
Expression Evidence

scholarly journals Inactivation of fission yeast Erh1 de-represses pho1 expression: evidence that Erh1 is a negative regulator of prt lncRNA termination

RNA ◽  
2020 ◽  
Vol 26 (10) ◽  
pp. 1334-1344 ◽  
Author(s):  
Beate Schwer ◽  
Ana M. Sanchez ◽  
Stewart Shuman
Keyword(s):  
Fission Yeast ◽  
Negative Regulator ◽  
Expression Evidence
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