scholarly journals Dynamics of Red Blood Cell Indices among Donor Samples in Kisii County, Western Kenya

2020 ◽  
Vol 2 (1) ◽  
pp. 9-14
Author(s):  
Chrisphine Momanyi ◽  
Stanslaus Kiilu Musyoki ◽  
Benson Nyanchongi
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Room Temperature ◽  
Primary Objective ◽  
Red Cell ◽  
Cross Sectional ◽  
Western Kenya ◽  
Blood Samples ◽  
Cell Parameters ◽  
Cell Counts

Blood specimens are used to study the aetiology, diagnosis, treatment, prognosis, and prevention of blood diseases that includes research purposes. The primary health care worldwide calls for ideal systems that can provide accurate results but it is challenging to analyse the samples immediately. Thus, the primary objective of this study was to determine the changes in the red cell indices when stored at room temperatures. To accept such samples that have delayed after collections for some time as from zero (0) hours, at 6 hours, 12 hours, 24 hours and 48 hours requires testing and confirming the results validations. Therefore, this research analysed samples of blood to validate the results after storage at room temperature in different hours within 48 hours of storage at room temperature. Samples collected from donors aged 16-65 years at Kisii Teaching and Referral Hospital in Kisii County, Western Kenya were tested in this study. The technique of Flow cytometry was used to analyse results of blood obtained through Venepuncture procedure from donors and placed in 4 ml Ethylene Diamine Tetra Acetic (EDTA) Vacutainer tubes of standardised anticoagulant ratio. A cross-sectional study with laboratory experiment was employed to analyse the data between the month of April and August 2018. Percentile male and female blood samples were analysed at 48% and 52% respectively. The analytes of obtained were used to determine the two red blood cell parameters in controlled room temperature within 48 hours on Packed Cell Volume (PCV) and Total Red Cell counts. Statistical analysis with computerized applications package (SPSS, version 21.0) was used to determine the changes that occurred during the study with repeated Analysis of Variation (ANOVA). Comparative mean values were calculated for each blood samples. The results were insignificant at sixth and twelfth hours of storage for most indices except from the RBC total counts, which had a deviation between 6-48 hours. In conclusion blood sample analysis is safe for tests results up 12 hours of storage at room temperature when not tested immediately after collection. This study recommends further finds for Reticulocytes Production Index to assess bone marrow activities.

Correlation of Red Blood Cells and Platelets Parameters.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3764-3764
Author(s):  
Zoi Saouli ◽  
Georgia Kaiafa ◽  
Fotios Girtovitis ◽  
Zisis Kontoninas ◽  
George Ntaios ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Red Cell ◽  
Blood Cell Count ◽  
Distribution Width ◽  
Mean Values ◽  
Cell Parameters ◽  
The Mean

Abstract INTRODUCTION: Platelet along with red blood cell count is a part of complete blood cell count, one of the most frequent laboratory tests in medicine. Platelet distribution width, plateletcrit and mean platelet volume are three indices provided by hematological analyzers. There are few reports in literature regarding the correlation of these three parameters with red blood cell parameters. AIM: Aim of this study is to investigate the correlation between these platelets parameters and red cell parameters: hematocrit, mean corpuscular volume and red blood cell distribution width. METHODS: Three hundred and three healthy blood donor volunteers (176 men and 127 women, mean age 37,3 years) were included. None of them had any known hematological disease in the past. The parameters mentioned above were measured by the automated hematological analyzer Coulter®LH780. RESULTS: The mean values for platelets were: PCT: 0,25±0,11%, MPV: 8,11±1,94 fL and PDW: 15,89±2,74%. The mean values for their parallel red blood cell parameters were: HCT: 40,55±2,63%), MCV: 91±4,17 fL, RDW: 13,3±1,35% Statistical and regression analysis including the correlation coefficient between platelet and red cell parameters as well as Student’s t-test was carried out. CONCLUSIONS: There seems to be no significant correlation between HCT and PCT. MCV and MPV were not correlated significantly as well, indicating that red blood cell and platelet sizes are independent. But there is a statistically significant correlation between RDW and PDW (r: 0,68, p<0,01) demostrating that anisocytosis of red blood cells and platelets might occur simultaneously. Based on these observations, further more studies should be carried out for the correlation between platelets and red blood cell indices in certain disorders.

scholarly journals Weight-length relationship, condition factor and blood parameters of farmed Cichla temensis Humboldt, 1821 (Cichlidae) in central Amazon

2011 ◽  
Vol 9 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Marcos Tavares-Dias ◽  
Adriana Maria de Castro Monteiro ◽  
Elizabeth Gusmão Affonso ◽  
Kárlia Dalla Santa Amaral
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Condition Factor ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Central Amazon ◽  
Cell Parameters ◽  
Cell Counts ◽  
Length Relationship

Farming of native carnivore fish species has drawn attention due to their promising use in aquaculture. Among these species, tucunaré of the genus Cichla stand out, them being of high economical interest for sport fishing and Amazon's industry of ornamental fish. The present study describes the weight-length relationship (WLR), relative condition factor (Kn), red blood cell parameters, thrombocytes and leukocytes count of Cichla temensis Humboldt, 1821, farmed in central Amazon. Fish that underwent food training during fingerling culture received extruded ration containing 45% of crude protein during fattening, and had Kn with values from 0.925-1.199, which indicated good health condition during the culture. The equation obtained from the WLR was W = 0.0073Lt3.1435, indicating an isometric growth, which is the desirable for fish of fish farm. Red blood cell counts, total thrombocyte and leukocyte counts, hematocrit, hemoglobin concentration, mean corpuscular volume (MCV), concentration of mean corpuscular hemoglobin (MCHC), lymphocytes, monocytes and neutrophils had intra-specific variation. A significant (p<0.001) positive correlation of the red blood cells number with the hemoglobin concentration and hematocrit was found. These are the first sets of blood parameters for C. temensis and could be used as reference for comparison in further studies to evaluate the health status of this fish in different environments, because assessment of these parameters may be used as quick tool for diagnosing diseases, stress and malnutrition.

scholarly journals The Impact of Obesity and Insulin Resistance on Iron and Red Blood Cell Parameters: A Single Center, Cross-Sectional Study

2014 ◽  
Vol 31 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Esma Altunoğlu ◽  
Cüneyt Müderrisoğlu ◽  
Füsun Erdenen ◽  
Ender Ülgen ◽  
M. Cem Ar
Keyword(s):  
Insulin Resistance ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Single Center ◽  
Cross Sectional ◽  
Cell Parameters ◽  
The Impact

scholarly journals Evaluation of Red Blood Cell Parameters as a Biomarker for Long-Term Glycemic Control Monitoring Among Type 2 Diabetic Patients in Southwest Ethiopia: A Cross-Sectional Study

2021 ◽  
Author(s):  
Misganaw Asmamaw ◽  
Tariku Sime ◽  
Kumsa Kene ◽  
Minale Fekadie ◽  
Muluken Teshome ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Red Cell Distribution Width ◽  
Mean Corpuscular Hemoglobin ◽  
Red Cell ◽  
Cell Distribution ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Cell Parameters

Abstract Background: Type 2 Diabetes Mellitus (T2 DM) is a common type of DM characterized by hyperglycemia. Glycation of hemoglobin and related proteins in DM can affect the physiological and structural properties of red blood cells. Although glycated hemoglobin (HbA1c) test continues to be the gold standard for the assessment of long-term glycemic control accessibility and affordability of the test in routine diagnosing service are still limited in developing countries. Hence, this study was aimed to assess red blood cell parameters as a biomarker for long-term glycemic monitoring among T2 DM patients.Methods: Facility-based cross-sectional study through a consecutive sampling technique was conducted among 124 T2 DM patients at the chronic illness follow-up clinic of Jimma Medical Center (JMC) from July 27 to August 31, 2020. A structured questionnaire was used to collect socio-demographic and clinical-related data. Five milliliters of the blood specimen was collected from each eligible T2 DM patient. HbA1c and red blood cell parameters were determined by Cobas 6000 and DxH 800 fully automated analyzers respectively. Data were entered into Epi-data software version 3.1 and exported to SPSS 25 version for analysis. Independent t-test and Pearson’s correlation coefficient were used to address the research questions. A P-value < 0.05 was considered statistically significant. Results: The mean age of study participants was 51.84± 11.6 years. 60.5% of T2 DM patients were in poor glycemic control. There was a significant mean difference between good and poor glycemic controlled T2 DM patients in red blood cell count (4.79±0.5 vs 4.38±0.8), hemoglobin (14.13±1.4 vs 13.60±1.6), mean corpuscular volume (89.52±4.7 vs 92.62±7.5), mean corpuscular hemoglobin (29.63±1.6 vs 30.77±2.9), and red cell distribution width (13.68±1.1 vs 14.63±1.2) respectively. Red blood cell count was inversely correlated (r=-0.280, p=0.002) with HbA1c while mean corpuscular volume (r=0.267, p=0.003), mean corpuscular hemoglobin (r=0.231, p=0.010), and red cell distribution width (r= 0.496, p=0.000) were positively correlated with level of HbA1c. Conclusion: Red cell count, mean corpuscular volume, mean corpuscular hemoglobin, and red cell distribution width could be useful indicators to monitor the glycemic status of T2 DM patients instead of HbA1c, though large prospective studies should be considered.

scholarly journals Microangiopathic Hemolytic Anemia Associated with Radiation-Induced Hemangiosarcomas

1980 ◽  
Vol 17 (4) ◽  
pp. 443-454 ◽  
Author(s):  
A. H. Rebar ◽  
F. F. Hahn ◽  
W. H. Halliwell ◽  
D. B. DeNicola ◽  
S. A. Benjamin
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Red Cell ◽  
Microangiopathic Hemolytic Anemia ◽  
Neoplastic Tissue ◽  
Cell Parameters ◽  
Radiation Induced

A retrospective study of red blood cell parameters in 53 dogs with experimental radiation-induced hemangiosarcoma showed 24 had anemia. Morphologic alterations in red blood cells in peripheral blood films from anemic dogs included signs of regeneration (anisocytosis and polychromasia), hypochromasia, red cell fragmentation and acanthocytosis. The degree and type of red cell changes varied from dog to dog and generally correlated with the principal site of tumor involvement. Blood from dogs with tumors principally involving liver had red cell regeneration, fragmentation and acanthocytosis. Blood from dogs with tumors primarily involving the heart had only red cell fragmentation. Blood films from dogs with skeletal and pulmonary hemangiosarcomas were similar to blood films from dogs with hepatic hemangiosarcoma except that red cell alterations generally were less severe. Scanning and transmission electron micrographic evaluation of neoplastic tissue showed large amounts of fibrin within neoplastic vascular sinuses and disruption and distortion of red blood cells traversing these abnormal vascular beds. The red blood cell fragmentation syndrome associated with radiation-induced hemangiosarcomas therefore was considered to be a microangiopathic hemolytic anemia of localized origin.

scholarly journals Prevalence of Anaemia after Initiation of Antiretroviral Therapy among HIV-infected Patients Attending University of Calabar Teaching Hospital Calabar, Nigeria

2019 ◽  
pp. 1-7
Author(s):  
A. O. Okafor ◽  
E. C. Akwiwu ◽  
J. O. Akpotuzor
Keyword(s):  
Antiretroviral Therapy ◽  
Teaching Hospital ◽  
Venous Blood ◽  
Haemoglobin Concentration ◽  
Red Cell ◽  
Duration Of Treatment ◽  
Cross Sectional ◽  
Cell Parameters ◽  
Control Subjects ◽  
Cell Counts

Aims: To investigating some demographic variables and red cell parameters of infected persons already accessing antiretroviral therapy with a view to identifying sub- groups with higher proportions of anaemia.  Study Design:  Cross-sectional study. Place and Duration of Study: University of Calabar Teaching Hospital Calabar Nigeria, between August 2017 and July 2018. Methodology: Subjects comprised 60 male and female HIV-infected adults attending University of Calabar Teaching Hospital Calabar, and equal number of age and sex-matched control subjects who were HIV sero-negative as at the time of this study. The infected persons were being treated with either Tenofovir+Lamivudine+Efavirenz (TLE) or Lamivudine+Zidovudine+Nevirapine (LZN). A pre-tested structured questionnaire was administered by two trained interviewers which captured the bio-data, sociodemographic variables and therapy-related information. Venous blood was collected aseptically by standard phlebotomy into appropriate sample containers for CD4 and red cell counts by automation. Results: The proportion of anaemia occurring between TLE and LZN users was observed to be in the ratio of 2:3. The RBC count, haematocrit and haemoglobin concentration were significantly lower (p=.000), while MCV, MCH and RDW were significantly higher (p=.000) in subjects infected with HIV compared to the control subjects, Duration of treatment beyond 6 years significantly raised both MCV (p=.007) and MCH (p=.006) compared to the first 3 years of treatment commencement, while the MCV, MCH and RDW-SD were significantly higher (p=.003, .014 and .018 respectively) among LZN users compared to those on TLE. Conclusion: Human immunodeficiency virus infection triggers pathologic mechanisms that culminate into anaemia. While the use of antiretroviral therapy appears to gradually resolve this derangement, the adverse effects of some of the antiretroviral agents contribute to the persistence of anaemia particularly with increasing years of treatment.

scholarly journals Vitamin B12 Status and Optimal Range for Hemoglobin Formation in Elite Athletes

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1038 ◽  
Author(s):  
Jarosław Krzywański ◽  
Tomasz Mikulski ◽  
Andrzej Pokrywka ◽  
Marcel Młyńczak ◽  
Hubert Krysztofiak ◽  
...  
Keyword(s):  
Blood Cell ◽  
Vitamin B12 ◽  
Red Blood Cell ◽  
Elite Athletes ◽  
Track And Field ◽  
Optimal Range ◽  
Blood Samples ◽  
Hemoglobin Synthesis ◽  
Cell Parameters

Background: Athletes and coaches believe in the ergogenic effect of vitamin B12 (which results from enhanced erythropoiesis) and they often insist on its unjustified supplementation. Therefore, our study aimed to assess the vitamin B12 status in Polish elite athletes and its influence on red blood cell parameters. Methods: In total, 1131 blood samples were collected during six years from 243 track and field athletes divided into strength and endurance groups, as well as according to the declared use of vitamin B12 injections. Results: An average vitamin B12 concentration in all subjects was 739 ± 13 pg/mL, with no cases of deficiency. A weak but significant relationship was found between vitamin B12 and hemoglobin concentrations. A significant increase in hemoglobin appeared from very low vitamin B12 concentration and up to approx. 400 pg/mL, while hemoglobin did not significantly change from 700 pg/mL and onwards. Vitamin B12 injections were used by 34% of athletes, significantly more often by endurance than by strength athletes. In athletes who declared no use of injections, a higher concentration of vitamin B12 was observed in the endurance group. Conclusion: The main finding of the present study is the determination of the range of vitamin B12 concentration which may favor better hemoglobin synthesis in athletes. They should regularly monitor vitamin B12 concentration and maintain the range of 400–700 pg/mL as it may improve red blood cell parameters. We might suggest application of a supplementation if necessary. Special attention is required in athletes with a vitamin B12 concentration below 400 pg/mL.

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

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