scholarly journals CONDITION OF HUMORAL IMMUNITY IN PATIENTS WITH SYSTEMIC DISEASES OF CONNECTIVE TISSUE WITH ACTIVATED CHRONIC SIMPLE HERPETIC INFECTION

2020 ◽  
pp. 64-71
Author(s):  
Igor Gaiduchok ◽  
Yuriy Fedorov ◽  
Khrystyna Lishchuk-Yakymovych ◽  
Roman Pukalyak
Keyword(s):  
Connective Tissue ◽  
Humoral Immunity ◽  
Genetic Diagnosis ◽  
Connective Tissue Diseases ◽  
Active Phase ◽  
Control Group ◽  
Systemic Diseases ◽  
Autoimmune Pathology ◽  
Latent Phase ◽  
Hsv 1

The aim - to study and to analyze the indicators of the humoral link of the immune system in patients with systemic connective tissue diseases with chronic activated HSV 1/2 infection and to assess the effect of the revealed disorders on the development of autoimmune pathology. Materials and methods. We examined 380 patients with systemic diseases of the connective tissue (systemic lupus erythematosus, systemic vasculitis, rheumatoid arthritis, psoriasis) with chronic HSV 1/2 infection at the age of 21-67 years. In 45 of them, using the modern molecular genetic diagnosis of herpes simplex, the active phase of chronic HSV 1/2 infection was verified based on the results of the polymerase chain reaction, and in the other 335, the latent phase was verified. The control group consisted of 20 healthy individuals of the appropriate age and gender. In patients with systemic connective tissue diseases with chronic HSV 1/2 infection, the humoral immunity indices were studied: levels of complement component C3 and IFN-a in saliva and blood and acquired: concentration of common major immunoglobulinins: IgG, IgM IgA, IgE; specific IgM and IgG to HSV 1/2; levels of cryoglobulins, cryofibrinogen, CІC in serum. Statistical processing of the results of the study was carried out using generally accepted methods of variation statistics. Results. In patients with systemic diseases of connective tissue with chronic HSV 1/2 infection, activation of the humoral immunity was detected, it was especially evident in the active phase of the infection and was characterized by an increase in the antibody production of specific IgM HSV 1/2 and IgG HSV 1/2, аn increase in the levels of CIC, IgM, IgA and IgE, cryoprecipitation proteins, IFN-a in the blood. In the latent phase of HSV 1/2 infection the levels of IgG production of HSV 1/2, IgE, CEC, cryofibrinogen and cryoglobulins in the blood serum of these patients increased. Conclusions. The presence of chronic activated HSV 1/2 infection in patients with systemic diseases of the connective tissue causes activation of the humoral immunity, creates the conditions for the development of autoimmune processes.

scholarly journals Serum KL-6 as predictive and prognostic marker of interstitial lung disease in childhood connective tissue diseases: a pilot study

Reumatismo ◽  
2021 ◽  
Vol 73 (3) ◽  
Author(s):  
R. El-Beheidy ◽  
A.M. Domouky ◽  
H. Zidan ◽  
Y.A. Amer
Keyword(s):  
Interstitial Lung Disease ◽  
Connective Tissue ◽  
Lung Disease ◽  
Lupus Erythematosus ◽  
Connective Tissue Diseases ◽  
Control Group ◽  
Connective Tissue Disorders ◽  
Juvenile Systemic Lupus Erythematosus ◽  
Median Serum ◽  
Juvenile Systemic Sclerosis

This study was aimed to evaluate serum KL-6 levels to determine if this marker can be used for diagnosing and assessing severity of interstitial lung disease (ILD) in children with connective tissue disorders. In total, 40 patients [18 patients with juvenile systemic lupus erythematosus (JSLE), 10 patients with juvenile idiopathic arthritis (JIA), 8 patients with juvenile mixed connective tissue disease (JMCTD), 3 patients with juvenile systemic sclerosis (JSSc), and 1 patient with juvenile dermatomyositis (JDM)] and 20 healthy controls were included in this study. Age, sex, and duration of CTD and ILD (if any) were recorded. Blood samples from all the patients and controls were examined by ELISA. 20 of the 40 patients with CTD (50%) had ILD, 12 were mild and 8 were severe as assessed by spirometry. The median serum KL-6 level was 102.7 U/mL (76.1-180.8) in the CTD with severe ILD group, 72.2 U/mL (58.4- 100.5) in the CTD with mild ILD group, 56.7 U/mL (35.8-68.5) in the CTD without ILD group, and 52.3 U/mL (32.8-62.4) in the control group. KL-6 levels were significantly higher in the CTD with ILD (p<0.05), at a cutoff of 63.4 U/ml identified by ROC curve, serum KL-6 showed a sensitivity of 95.2% and specificity of 89.7%. KL-6 is a valuable biomarker for diagnostic purposes and to detect severity in ILD in childhood CTD.

Safety of hydroxychloroquine in pregnant patients with connective tissue diseases: A study of one hundred thirty-three cases compared with a control group

2003 ◽  
Vol 48 (11) ◽  
pp. 3207-3211 ◽  
Author(s):  
Nathalie Costedoat-Chalumeau ◽  
Zahir Amoura ◽  
Pierre Duhaut ◽  
Du Le Thi Huong ◽  
Djamel Sebbough ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Diseases ◽  
Control Group

scholarly journals COVID-19 and rheumatic autoimmune systemic diseases: report of a large Italian patients series

2020 ◽  
Vol 39 (11) ◽  
pp. 3195-3204 ◽  
Author(s):  
Clodoveo Ferri ◽  
◽  
Dilia Giuggioli ◽  
Vincenzo Raimondo ◽  
Massimo L’Andolina ◽  
...  
Keyword(s):  
Immune System ◽  
General Population ◽  
Connective Tissue ◽  
Inflammatory Arthritis ◽  
Systemic Disease ◽  
Management Strategies ◽  
Connective Tissue Diseases ◽  
Systemic Diseases ◽  
High Exposure ◽  
Disease Modifying

Abstract Introduction Covid-19 infection poses a serious challenge for immune-compromised patients with inflammatory autoimmune systemic diseases. We investigated the clinical-epidemiological findings of 1641 autoimmune systemic disease Italian patients during the Covid-19 pandemic. Method This observational multicenter study included 1641 unselected patients with autoimmune systemic diseases from three Italian geographical areas with different prevalence of Covid-19 [high in north (Emilia Romagna), medium in central (Tuscany), and low in south (Calabria)] by means of telephone 6-week survey. Covid-19 was classified as (1) definite diagnosis of Covid-19 disease: presence of symptomatic Covid-19 infection, confirmed by positive oral/nasopharyngeal swabs; (2) highly suspected Covid-19 disease: presence of highly suggestive symptoms, in absence of a swab test. Results A significantly higher prevalence of patients with definite diagnosis of Covid-19 disease, or with highly suspected Covid-19 disease, or both the conditions together, was observed in the whole autoimmune systemic disease series, compared to “Italian general population” (p = .030, p = .001, p = .000, respectively); and for definite + highly suspected diagnosis of Covid-19 disease, in patients with autoimmune systemic diseases of the three regions (p = .000, for all comparisons with the respective regional general population). Moreover, significantly higher prevalence of definite + highly suspected diagnosis of Covid-19 disease was found either in patients with various “connective tissue diseases” compared to “inflammatory arthritis group” (p < .000), or in patients without ongoing conventional synthetic disease-modifying anti-rheumatic drugs treatments (p = .011). Conclusions The finding of a higher prevalence of Covid-19 in patients with autoimmune systemic diseases is particularly important, suggesting the need to develop valuable prevention/management strategies, and stimulates in-depth investigations to verify the possible interactions between Covid-19 infection and impaired immune-system of autoimmune systemic diseases. Key Points• Significantly higher prevalence of Covid-19 is observed in a large series of patients with autoimmune systemic diseases compared to the Italian general population, mainly due to patients’ increased susceptibility to infections and favored by the high exposure to the virus at medical facilities before the restriction measures on individual movement.• The actual prevalence of Covid-19 in autoimmune systemic diseases may be underestimated, possibly due to the wide clinical overlapping between the two conditions, the generally mild Covid-19 disease manifestations, and the limited availability of virological testing.• Patients with “connective tissue diseases” show a significantly higher prevalence of Covid-19, possibly due to deeper immune-system impairment, with respect to “inflammatory arthritis group”.• Covid-19 is more frequent in the subgroup of autoimmune systemic diseases patients without ongoing conventional synthetic disease-modifying anti-rheumatic drugs, mainly hydroxyl-chloroquine and methotrexate, which might play some protective role against the most harmful manifestations of Covid-19.

scholarly journals The Serum Cell-Free microRNA Expression Profile in MCTD, SLE, SSc, and RA Patients

2020 ◽  
Vol 9 (1) ◽  
pp. 161 ◽  
Author(s):  
Barbara Stypinska ◽  
Anna Wajda ◽  
Ewa Walczuk ◽  
Marzena Olesinska ◽  
Aleksandra Lewandowska ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Signaling Pathway ◽  
Expression Profile ◽  
Mirna Expression ◽  
Lupus Erythematosus ◽  
Connective Tissue Diseases ◽  
Mirna Expression Profile ◽  
Expression Level ◽  
Control Group ◽  
Mirna Expression Level

Mixed connective tissue disease (MCTD) is a rare disorder characterized by symptoms that overlap two or more Autoimmune Connective Tissue Diseases (ACTDs). The aim of this study was to determine whether miRNAs participating in the TLRs signaling pathway could serve as biomarkers differentiating MCTD or other ACTD entities from a healthy control group and between groups of patients. Although the selected miRNA expression level was not significantly different between MCTD and control, we observed that miR-126 distinguishes MCTD patients from all other ACTD groups. The expression level of miRNAs was significantly higher in the serum of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients compared to controls. The miR-145 and -181a levels distinguished RA from other ACDT patients. miR-155 was specific for SLE patients. MiR-132, miR-143, and miR-29a distinguished RA and SLE patients from the systemic sclerosis (SSc) group. Additionally, some clinical parameters were significantly related to the miRNA expression profile in the SLE group. SLE and RA are characterized by a specific serum expression profile of the microRNAs associated with the Toll-like receptors (TLRs) signaling pathway. The analysis showed that their level distinguishes these groups from the control and from other ACTD patients. The present study did not reveal a good biomarker for MCTD patients.

scholarly journals Evolution of Mixed Connective Tissue Disease in 7 Years Old Child: Clinical Case

2020 ◽  
Vol 19 (3) ◽  
pp. 214-219
Author(s):  
Tamara P. Makarova ◽  
Khakim M. Vakhitov ◽  
Dina R. Sabirova ◽  
Dinara I. Sadykova ◽  
Liliya R. Khusnutdinova ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Disease ◽  
Lupus Erythematosus ◽  
Mixed Connective Tissue Disease ◽  
Clinical Case ◽  
Clinical Signs ◽  
Systemic Scleroderma ◽  
Systemic Diseases ◽  
Autoimmune Pathology ◽  
Short Period

Background. Mixed connective tissue disease (Sharp syndrome) is the rare chronic autoimmune pathology combining various features of systemic lupus erythematosus, systemic scleroderma, rheumatoid arthritis, dermatomyositis and high antibody titer to nuclear ribonucleoprotein. The mixed connective tissue disease may evolve into other systemic diseases over time. Description of any cases of mixed connective tissue disease and its evolution in Russian patients has not been published previously.Clinical Case Description. The results of observations of the child with clinical and immunological signs of the mixed connective tissue disease followed by the progression of systemic scleroderma symptoms and development of Sjogren's syndrome in the short period of time are presented in the article. Improvement (such as pain attenuation, increase in volume of movements in affected joints, decrease of Raynaud syndrome manifestations duration) was observed on treatment (methotrexate 10 mg/week with subsequent addition of prednisolone 0.75 mg/kg/day).Conclusion. Timely diagnostics of clinical signs of the systemic diseases debut is crucial for correct patient routing and for achieving of disease improvement.

scholarly journals Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Alejandro Caro Pérez ◽  
Sarita Kumble ◽  
Krishnanand D. Kumble ◽  
M. Consuelo Alonso Cañizal ◽  
Luis M. Jiménez Jiménez ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Connective Tissue ◽  
Infectious Diseases ◽  
Healthy Subjects ◽  
Laboratory Data ◽  
Connective Tissue Diseases ◽  
Control Group ◽  
Critical Importance ◽  
Clinical Tool ◽  
Cohen's Kappa

The performance of immunoassays for the detection of autoantibodies is of critical importance in the diagnosis and assessment of patients with autoimmune connective tissue diseases (ACTD). Our objective was to compare the features of two multiplexed assays—INNO-LIA ANA and Gennova-PictArray ENA ELISA—for measurement of multiple autoantibodies and their utility as a clinical tool in ACTD diagnosis. The antigens included SS-A/Ro (60 and 52), SSB/La, Sm, Sm/RNP, CENP-B, Jo-1, and Scl-70. Stored sera from 85 ACTD patients and 80 controls consisting of patients with vasculitis, rheumatoid arthritis and infectious diseases, as well as healthy subjects were analyzed jointly with clinical and laboratory data. Agreement between the two methods varied between 58 and 99% (Cohen’s kappa: 0.21–0.71) mostly for SSA and SSB. The frequency of specific autoantibodies measured using the two methods was more variable for SSA, SSB, and RNP/Sm. There were a higher number of ambiguous results when using INNO-LIA. The optimized cut-off values of the Gennova-PictArray resulted in over 99% specificities in samples obtained from the control group. Sensitivity patterns were more accurate in Gennova-PictArray than in INNO-LIA, as suggested in previously reported studies. A third method could be applied to determine which of the two methods is more accurate.

scholarly journals Chemerin, Resistin, and Adiponectin in Patients with Connective Tissue Diseases

2018 ◽  
Vol 37 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Bogna Grygiel-Górniak ◽  
Teresa Grzelak ◽  
Krystyna Czyźewska ◽  
Mariusz Puszczewicz
Keyword(s):  
Connective Tissue ◽  
Roc Curve ◽  
Metabolic Disorders ◽  
Connective Tissue Diseases ◽  
Area Under The Curve ◽  
Control Group ◽  
Reactive Protein ◽  
State Present ◽  
Inflammatory State ◽  
Wbc Count

SummaryBackground:The exact role of cytokines in inflammation and metabolic disorders in case of connective tissue diseases (CTDs) is under discussion.Methods:In this study, we intended to find the relationship between the selected cytokines in inflammatory and metabolic disorders in patients with CTDs (n=55) and compared the results with those of control group subjects (n=25) matched by age and body mass. We estimated their nutritional status by the bioimpedance method. The levels of basic biochemical parameters and the levels of adiponectin, resistin, and chemerin were also estimated. Multiple regressions and area under the curve in receiver operating characteristic (AUC–ROC) curve were used to find the associations of aforementioned parameters.Results:Patients with CTDs exhibited higher levels of chemerin than that of control group subjects. We found an inverse relationship between chemerin, RBC count, and hemoglobin levels. The concentration of adiponectin inversely correlated with the levels of platelets and concentrations of glucose and triglycerides as well as the erythrocyte sedimentation rate, whereas the concentration of resistin was positively correlated with WBC count, C-reactive protein (CRP), and the amount of used oral glucocorticosteroids. The mean ± standard deviation for the AUC–ROC curve in case of chemerin was the highest (AUC–ROC=0.714,p=0.0005) than that of both resistin and adiponectin.Conclusions:Chemerin and resistin levels are related to the inflammatory state in patients with CTDs, whereas adiponectin levels seem to be correlated with a protective effect. Chemerin can be considered as a marker differentiating a proinflammatory state present in CTDs.

Rehabilitation in joint and connective tissue diseases. 1. Systemic diseases

1995 ◽  
Vol 76 (5) ◽  
pp. S32-S40 ◽  
Author(s):  
Neal Alpiner ◽  
Terry H. Oh ◽  
Steven R. Hinderer ◽  
Victoria A. Brander
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Diseases ◽  
Systemic Diseases

scholarly journals The Prevalence of Antinuclear Antibodies in Patients with Sarcoidosis

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Senol Kobak ◽  
Hatice Yilmaz ◽  
Fidan Sever ◽  
Arzu Duran ◽  
Nazime Sen ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Healthy Volunteers ◽  
Antinuclear Antibodies ◽  
Laboratory Data ◽  
Connective Tissue Diseases ◽  
Control Group ◽  
Rheumatologic Diseases ◽  
Radiological Data ◽  
Dsdna Antibody ◽  
Healthy Control

Introduction. Sarcoidosis, which is a chronic inflammatory granulomatous disease, can mimic different rheumatologic diseases including connective tissue diseases. Antinuclear antibodies are the markers used for connective tissue diseases.Aim. To determine antinuclear antibody frequency and any possible correlation with clinical and laboratory data in sarcoidosis patients.Material and Method. Forty-two sarcoidosis patients, 45 rheumatoid arthritis patients, and 45 healthy volunteers who were followed up in rheumatology outpatient clinic were included in this study. Demographic, clinical, serological, and radiological data of all patients were recorded. Antinuclear antibodies were determined with indirect immunofluorescent method and 1/100 titration was accepted as positive. The cases that were ANA positive were evaluated with immunoblot method.Results. Average age of the 42 patients (10 males) with sarcoidosis was 45.2 (20–70 years), and average disease duration was 3.5 years. ANA positivity was detected in 12 (28.5%) patients with sarcoidosis (1/100 in 10 patients, 1/320 in two patients), in 19 of RA patients (42.2%), and in two of healthy volunteers in low titer (P<0.001). In the subgroup analysis made by immunblot test, one patient had anticentromere antibody, one had anti-Ro antibody, one had anti-Scl-70 antibody, one had anti-dsDNA antibody, and eight patients were negative. The two patients who had anticentromere and anti-Scl-70 antibodies had also Sjögren’s syndrome and scleroderma diagnosis, respectively.Discussion. The prevalence of ANA in patients with sarcoidosis was found to be significantly higher than healthy control group and lower than RA patients. This result shows that ANA may have an important role in the pathogenesis of sarcoidosis and also could be important in revealing the overlap syndromes of sarcoidosis-connective tissue diseases. Further studies with larger series are necessary in this subject.

scholarly journals Evaluation strength of the supporting apparatus of the lens at combination of age-related cataract with involutional changes in connective tissue

2021 ◽  
Vol 14 (1) ◽  
pp. 5-13
Author(s):  
Evgenii L. Sorokin ◽  
Yaroslav V. Belonozhenko ◽  
Sofia V. Krivko ◽  
Oleg V. Danilov
Keyword(s):  
High Resolution ◽  
Connective Tissue ◽  
Prognostic Value ◽  
Main Group ◽  
Control Group ◽  
Systemic Diseases ◽  
Eye Injuries ◽  
Age Related ◽  
Ciliary Processes ◽  
The Difference

AIM: The clinical evaluation of zonules condition in patients with age-related cataracts without weak zonular support signs against the background of connective tissue somatic involutional changes. MATERIALS AND METHODS: The main group consisted of 70 patients (70 eyes) with connective tissue involutional somatic pathology without concomitant eye pathology, eye injuries, and decompensated systemic diseases; the control group included 60 people (60 eyes) with age-related cataracts without connective tissue involutional pathology. Using ocular echography (Aviso S, Quantel Medical, France) with high resolution (50 MHz) sensor, we estimated the ciliary processes to lens equator distance symmetry in 2 main meridians (of 6 and 12 hours). Its equal value in 2 opposite meridians or difference less than 0.3 mm between them was considered as the sign of symmetry; the difference of 0.3 mm and more was a sign of asymmetry. RESULTS: The presence of ciliary processes to lens equator distance asymmetry between the meridians was revealed in 28 eyes in the main group (40%); in 14 of the eyes with asymmetry ranging from 0.4 and more, a 1st degree lens subluxation was revealed intraoperatively. CONCLUSIONS: The presence of ciliary processes to lens equator distance asymmetry indicates subclinical involutional changes in the lens ligament apparatus, which has a prognostic value for choosing a model of an intraocular lens to be implanted.

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