Nonalcoholic Fatty Liver Disease and Vitamin E - A Promising Relationship?

2019 ◽  
Vol 70 (1) ◽  
pp. 78-83
Author(s):  
Alexandra Totan ◽  
Daniela Gabriela Balan ◽  
Daniela Miricescu ◽  
Radu Radulescu ◽  
Iulia Ioana Stanescu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Therapeutic Potential ◽  
Plga Nanoparticles ◽  
Diet Group ◽  
Normal Diet ◽  
Oxidative Stress Biomarkers ◽  
Promising Alternative ◽  
Nonalcoholic Fatty Liver ◽  
High Caloric Diet

Oxidative stress (OS) plays an important role in NAFLD molecular mechanism. Nanoencapsulation represents a novel strategy to enhance therapeutic potential of conventional drugs. Our study analyses the encapsulated vitamin E effect on lipid metabolism and oxidative stress biomarkers in NAFLD rats. Animals were divided into 3 groups : G1 - the normal diet group; G2- the high caloric diet group ; G3 - high-caloric diet group receiving PLGA-vit E, 50 mg / kg. Serum advanced human oxidative protein (AOPP), total antioxidant capacity (TAC) and vitamin E were analysed using ELISA technique. Our results showed significant increase of G2 GPT, ALP, GGT, TG, glucose, TC and AOPP, versus G1 ( P [ 0.05) and a significant decrease of G2 serum TAC and vitamin E versus G1 results ( p = 0.01 and 0.01). Vitamin E nanoparticles (G3) caused a significant increase of TAC and significant decrease of serum AOPP, versus G2 (p [ 0.01). Results showed a significant reduction of GPT, GGT, ALP, TG and total cholesterol ( p [0.05) in G3 versus G2. PLGA nanoparticles should be considered an attractive and promising alternative to improve the bioavailability and biological activity of vitaminE.

scholarly journals Oxidative Stress in Preterm Neonates: An Analysis of Oxidative Stress Biomarkers and Antioxidant Profiles

2020 ◽  
Author(s):  
Shobha S Pajai ◽  
Apurva P Bezalwar
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Cord Blood ◽  
Defense Mechanism ◽  
Preterm Neonates ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Study Results ◽  
Preterm Babies

Introduction: Oxidative stress is a complex event determined genetically and induced by an in- utero stressor. Oxidants are composed of reactive free radicals like Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) which are manifested by several macromolecules of lipid, protein and DNA, causing deleterious effects in several organs. Antioxidant defense mechanism and its ability to be induced by hyperoxia is relatively impaired in preterm neonates. Aim: To study oxidative stress and antioxidants in preterm neonates. Materials and Methods: This study is an observational analytical study, which included preterm babies (25 males and 20 females) delivered vaginally from October 2012 to October 2013. Cord blood was collected in citrate bulbs immediately after vaginal delivery and stored at 4°C until processed. Malondialdehyde (MDA), Nitrates, Vitamin C and Vitamin E, levels were measured in cord blood. Statistical z-test was applied. Results: High levels of oxidative stress biomarkers like MDA and Nitrites along with decreased levels of antioxidants, Vitamin C and Vitamin E in preterm neonates was observed. MDA and Nitrates levels were significantly higher in males (p<0.05) than females. Vitamin C and Vitamin E levels were not significant (p>0.05) in both. Conclusion: This study results may conclude that preterm neonates have more oxidative stress especially in males affecting their life survival.

Abstract 313: Protective Effects of Iron-Restricted Food against Diabetic Nephropathy in db/db Mice

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Hideaki Enomoto ◽  
Yasumasa Ikeda ◽  
Soichiro Tajima ◽  
Yuki Izawa-Ishizawa ◽  
Yoshitaka Kihira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Iron Reduction ◽  
Protective Action ◽  
Iron Concentration ◽  
Iron Chelation ◽  
Diet Group ◽  
Normal Diet ◽  
Protective Effects ◽  
Iron Restriction

Background: Iron, an essential trace metal for organisms, catalyzes highly toxic hydroxyl radical via Fenton/Haber-Weiss reaction. We recently have clarified that iron chelation prevents the development of adipocyte hypertrophy through the reduction of oxidative stress in diabetic obese mice. It is suggested that iron reduction potentiates to be beneficial effects against diabetic complications. In the present study, we investigated the protective action of iron restriction against the progression of diabetic nephropathy. METHODS: We employed and divided male db/db mice of 8 weeks old age, a model of diabetic nephropathy, into 2 groups, normal diet group (Fe 100mg/kg food; ND) and low iron diet group (Fe 10mg/kg food; LID). After 8 weeks treatment, the mice were used for analysis. Results: LID group showed the decreased renal iron content (7.5±3.0 μg/g tissue vs 5.7±0.3 μg/g tissue, ND vs LID, p<0.05), serum iron concentration (213±15 μg/dL vs 157±14 μg/dL, ND vs LID, p<0.05) and hemoglobin level (13.5±0.7g/dL vs 10.4±0.5g/dL, ND vs LID, p<0.01). Urinary albumin excretion was significantly decreased in LID group compared to ND group (2.6±0.4mg/g·Cre vs 1.4±0.3mg/g·Cre, ND vs LID, p<0.05). In histological analysis, mesangial proliferation was ameliorated in db/db mice with LID (39% vs 28%, ND vs LID, p<0.05). LID reduced the deposition of collagen IV, fibronectin and desmin by 61%, 60%, and 42%, respectively, in glomeruli of db/db mice. Superoxide production, p22phox and NOX4 expression were also diminished in kidney of LID group. Conclusion: Iron restriction is suggested to prevent the development of diabetic nephropathy through the suppression of oxidative stress.

scholarly journals Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis?

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Mustafa Yalçın ◽  
Muhammed Ali Kıyak ◽  
Mesut Akarsu ◽  
Aslı Çelik ◽  
Göksel Bengi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Treatment Options ◽  
Diet Group ◽  
Normal Diet ◽  
The Other ◽  
Albino Rats ◽  
Necrosis Factor Alpha ◽  
Alcoholic Steatohepatitis ◽  
Tnf Α ◽  
And Oxidative Stress

Due to the lack of effective medical treatment for non-alcoholic steatohepatitis (NASH), we aimed to evaluate new treatment options. In particular, our goal was to investigate and compare the effects of N-acetylcysteine (NAC) and Adalimumab treatment on tumor necrosis factor alpha (TNF-α) and oxidative stress during the development of NASH in a rat model of the disease. Our study included a total of 35 female Wistar albino rats that were divided into 5 groups of 7 each, and evaluated over a 6 week period. One group received a normal diet, while the other four groups received a methionine and choline deficient (MCD) diet. One of the groups receiving the MCD diet did not take any medicine, while the other three were administered NAC, adalimumab, or a NAC/adalimumab combination therapy. NASH was successfully established in the MCD diet group. Levels of TNF-α were effectively suppressed in the three groups that received therapy. Even though adalimumab significantly enhanced suppression of TNF-α, the NASH score was suppressed to a more statistically significant extent in the groups receiving NAC. Our study showed that TNF-α and oxidative stress play an important role in NASH pathogenesis. The antioxidant agent, NAC, was found to be superior to the anti-TNF agent, Adalimumab, in the improvement of total NASH score. Although these drugs did not prevent the development of NASH, it was shown that they mildly reverse the NASH histopathology score, suggesting improvement of and overall liver function.

scholarly journals Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Aein Azarang ◽  
Omid Farshad ◽  
Mohammad Mehdi Ommati ◽  
Akram Jamshidzadeh ◽  
Reza Heidari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Steatosis ◽  
Bacillus Coagulans ◽  
Male Rats ◽  
Health Priorities ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Nonalcoholic Fatty Liver ◽  
Stress Markers ◽  
And Oxidative Stress

Background. Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Methods. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics ( 1 ∗ 10 9   CFU in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Results. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Conclusions. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics’ cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.

Comparison of grain-based diet supplemented with synthetic vitamin E and lucerne hay-based diet on blood oxidative stress biomarkers and lamb meat quality

2019 ◽  
Vol 177 ◽  
pp. 146-152
Author(s):  
Benjamin W.B. Holman ◽  
Gianluca Baldi ◽  
Surinder S. Chauhan ◽  
David L. Hopkins ◽  
Greg R. Seymour ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Meat Quality ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Synthetic Vitamin ◽  
Lamb Meat

scholarly journals Lycopene - tomato's secret weapon against oxidative stress

1970 ◽  
Vol 10 (4) ◽  
pp. 275-279 ◽  
Author(s):  
PD Sarkar ◽  
A Sahu ◽  
T Gupta
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Base Line ◽  
Lipid Peroxidation Product ◽  
Oxidative Stress Biomarkers ◽  
Singlet Oxygen Quenching ◽  
Key Words Oxidative Stress ◽  
Tomato Products

Back ground: Lycopene, 40 carbon acyclic carotenoid containing 11 conjugated double bonds, is a phytochemical found in tomatoes and other red fruits. Oxygen derived free radicals are the most reactive species and as an antioxidant lycopene has a singlet oxygen quenching ability twice as high as that of ?-carotene and 10 times higher that of ?-tocoferol, lycopene participate in a host of chemical reactions to protect critical cellular biomolecules including lipid, proteins and DNA. Materials and Methods: The present study include 30 subjects having oxidative stress, age between 40-60 years, nonsmoker, with no history of chronic systemic illness and no medication were taken as patients.30 patients matched healthy subjects were taken as control. All subjects were selected from outpatient department of NSCB Medical College Jabalpur M.P. After estimation of base line antioxidant enzyme and vitamins, we supplement 180 gm of tomato (products like soup, paste. ketchup) contain 12 mg of lycopene to the patient group. After 60 days of lycopene supplementation oxidative stress biomarkers like SOD, GPX, GR, GSH, lipid peroxidation product MDA and other antioxidant vitamins A, vitamin C, vitamin E were estimated in patient's blood sample. Results: The main result of the study revealed that lipid per oxidation product MDA was found to be decreased significantly but after lycopene supplementation levels were improved. The results of SOD , GPX, GR, GSH,Vitamin A ,Vitamin E and Vitamin C were significantly increased after lycopene supplementation, it indicates the improved antioxidant profile after the supplementation of lycopene. Conclusion: There was a significant decrease in oxidative stress after the supplementation of lycopene therefore the study suggest that body's internal production of antioxidant is not enough to neutralize all free radicals, so increased dietary intake of antioxidant lycopene in the form of tomato products is beneficial, which is easily available in developing country like India. Key Words: Oxidative stress; Lycopene; MDA; GSH; SOD; Vitamin C; Vitamin E DOI: http://dx.doi.org/10.3329/bjms.v10i4.9500 BJMS 2011; 10 (4): 275-279

Assessing the Therapeutic Potential of Red Cabbage (Brassica oleracea var. capitata) to Modulate Renal and Cardiac Oxidative Stress Biomarkers in Hypercholesterolemic Rabbits

2020 ◽  
Vol 16 (4) ◽  
pp. 521-527
Author(s):  
Faiza Ashfaq ◽  
Masood S. Butt ◽  
Ahmad Bilal ◽  
Kanza A. Awan ◽  
Hafiz A.R. Suleria
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Creatine Kinase ◽  
Aqueous Extract ◽  
Therapeutic Potential ◽  
Oxidative Stress Biomarkers ◽  
Red Cabbage ◽  
Dietary Shift ◽  
Creatine Kinase Mb ◽  
Cabbage Extract

Background: The epidemiological transition is directly associated with dietary shift towards empty calories. In contrary, cabbage fights against oxidative stress mediated health disorders owing to the presence of nutritive constituents and low caloric count. The study aimed to assess the efficacy of red cabbage and its aqueous extract against renal and cardiac oxidative stress in response to hypercaloric diet. Methods: Red cabbage and its aqueous extract were fed to normal rabbits (Study I) and hypercholesterolemic diet (1% cholesterol) induced oxidatively stressed rabbits (Study II) for 12-weeks. Serum specific biomarkers of renal and cardiac oxidative stress including creatinine, urea, creatine kinase (CK), creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) were measured. Tissues of kidney and heart were employed to assess the activities of superoxide dismutase (SOD) and catalase (CAT) alongside; lipid peroxidation was also studied. Results: Red cabbage extract depicted more decrement (p<0.05) in serum creatinine and urea levels up to 14.45 and 9.01% in oxidatively stressed rabbits (Study II), respectively. In kidney tissues, red cabbage extract showed an obvious reduction in lipid peroxidation up to 24.07% in Study II that ultimately restored SOD (11.38%) and CAT (16.72%) activities. On the other hand, red cabbage demonstrated promising aptitude (p<0.05) in mitigating cardiac compromised condition by down-regulating lipid peroxidation by 16.44%, which considerably decreased the serum levels of CK, CK-MB, LDH and AST. Conclusion: Relatively, red cabbage extract showed a relatively better response in controlling renal oxidative stress as compared to red cabbage; however, vice-versa was observed in case of cardiac oxidative stress.

Effect of black garlic on visceral fat, oxidative stress and insulin resistance in nonalcoholic fatty liver disease rats

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Alifia Mukti Fajrani ◽  
Mohamad Sulchan ◽  
Siti Fatimah Muis ◽  
Hery Djagat Purnomo ◽  
Kis Djamiatun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Liver Disease ◽  
Vitamin E ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Visceral Fat ◽  
Fatty Liver Disease ◽  
Content Type ◽  
Nonalcoholic Fatty Liver

Purpose This paper aims to determine the effect of black garlic (BG) on visceral fat, oxidative stress and insulin resistance (IR) compared with metformin and vitamin E in nonalcoholic fatty liver disease (NAFLD) rats. Design/methodology/approach A randomized post-test only design with control group was used in this study. Rats were given high-fat fructose diet enriched with 1.25% cholesterol and 0.5% cholic acid for eight weeks to induce NALFD condition. The administration of BG dose of 450 mg/200 gBW, 900 mg/200 gBW and 1350 mg/200 gBW with a comparative control of 45 mg/200 gBW of metformin and vitamin E of 9 IU/200 gBW were given for four weeks via oral gavage to reduce visceral fat, oxidative stress and improve IR. Statistical analyses were performed to examine differences between groups with one-way analysis of variance and nonparametrics test. Findings Rats given with three different doses of BG for four weeks did not reduce body weight from 244 ± 4.4 to 284 ± 4.6 g, 242 ± 2.5 to 272 ± 3.1 g and 240 ± 2.4 to 270 ± 3.6 g, respectively, but significantly reduced visceral fat (p = 0.001) on BG groups with 3.7 ± 1.3, 2.7 ± 0.7 and 1.8 ± 0.6 g, respectively. BG improved oxidative stress (p = 0.001) with malondialdehyde level 5.1 ± 0.2, 3.0 ± 0.06 and 2.3 ± 0.06 ng/mL, respectively, but did not better than vitamin E group 1 ± 0.03 ng/mL. Significant (p = 0.001) improvement on insulin resistance with homeostatic model assessment IR in BG groups were 5.3 ± 0.1, 4.4 ± 0.1 and 4 ± 0.1, respectively, but not as good as metformin group 3.7 ± 0.1. Research limitations/implications Based on the experiment, there are several limitations including small sample size, performed on animal models in a relatively short time, did not examine organosulfurs compound (OSC) content of BG specifically and OSC affects metabolism in NAFLD remains unclear and will require further investigation. Practical implications BG is a functional food made from heated fresh garlic owing to the Maillard reaction and the organosulfur compounds as antioxidants. The higher the dose of BG, the greater the improvement in visceral fat, oxidative stress and IR in model NAFLD rats. Social implications NAFLD is a liver disorder caused by excessive fat and energy intake, the treatment strategies among others through diet modification. Originality/value In model NAFLD rats, BG administration improved NALFD markers but did not better rather than the metformin and vitamin E result.

scholarly journals The Influence of Hyperlipidemia on Endothelial Function of FPN1 Tek-Cre Mice and the Intervention Effect of Tetramethylpyrazine

2018 ◽  
Vol 47 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Ming-Yue Sun ◽  
Miao Zhang ◽  
Shui-Ling Chen ◽  
Shu-Ping Zhang ◽  
Chun-Yu Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Function ◽  
Iron Overload ◽  
High Fat Diet ◽  
Iron Homeostasis ◽  
Diet Group ◽  
Normal Diet ◽  
High Fat ◽  
Excess Iron ◽  
Serum Hepcidin

Background/Aims: Systemic iron homeostasis is strictly governed in mammals; however, disordered iron metabolism (such as excess iron burden) is recognized as a risk factor for various types of diseases including AS (Atherosclerosis). The hepcidin-ferroportin axis plays the key role in regulation of iron homeostasis and modulation of this signaling could be a potential therapeutic strategy in the treatment of these diseases. TMP (Tetramethylpyrazine) has been reported to have therapeutical effect on AS. Here, we aimed to investigate the effect of iron overload under hyperlipidemia condition on the endothelial injury, inflammation and oxidative stress by employing FPN1 Tek-cre mouse model with or without TMP intervention. Methods: Subjects for this study were 80 FPN1 Tek-cre mice and 40 C57BL/6 mice and we randomly divided them into six groups: Group N: C57BL/6 mice with normal diet, Group M: C57BL/6 mice with high-fat diet, Group FN: FPN1 Tek-cre mice with normal diet, Group FNT: FPN1 Tek-cre mice with normal diet and TMP injection, Group FM: FPN1 Tek-cre mice with high-fat diet, Group FMT: FPN1 Tek-cre mice with high-fat diet and TMP injection. After seven days of treatment, blood samples were obtained to detect the levels of blood lipids, Hepcidin, NO, ET-1, ROS, MDA, SOD, IL-1, IL-6 and TNF-α respectively. The liver and aorta were used for testing the lipid deposition by using hematoxylin and eosin(HE). Results: Hyperlipidemia could cause iron overload in the aorta and increased serum hepcidin level, particularly in FPN1 Tek-cre mice, and can be reversed by TMP intervention. Knockout of Fpn1 induced increase of serum hepcidin, exacerbated endothelial dysfunction, oxidative stress and inflammatory response, particularly under hyperlipidemia condition. TMP intervention attenuated these processes. Conclusions: Our study signifies the potential application of certain natural compounds to ameliorating iron disorders induced by hyperlipidemia and protecting on endothelial function through modulation of hepcidin-ferroportin signaling.

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