scholarly journals Renoprotective Effects of PLGA Nanoparticles Loaded with Vitamin E in Wistar Rats, on Hypercaloric Diet

2020 ◽  
Vol 71 (6) ◽  
pp. 230-237
Author(s):  
Adriana Dinu ◽  
Bogdana Virgolici ◽  
Daniela Miricescu ◽  
Alexandra Totan ◽  
Elvira Gagniuc ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Male Rats ◽  
Antioxidant Vitamin ◽  
Standard Diet ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Stress Markers

Obesity is associated with renal lipomatosis, inflammation and oxidative stress. Vitamin E is a liposoluble antioxidant vitamin and PLGA (polylactic-CO-glycolic acid) nanoparticles (NPs) represent a delivery carrier of this vitamin to the abdominal viscera. The aim of this study is to evaluate the renal and systemic effects of PLGA- NPs loaded with vitamin E in high fat diet Wistar rats. Young heathy Wistar male rats (3months old, n=20) were fed for 4 weeks either with standard diet or with high caloric/fat diet. These two experimental groups were divided in two subgroups and for the following 21 days, one group received PLGA-NPs loaded with vitamin E (1 mg/kg) body and the other didn�t. Metabolic serum parameters, oxidative stress markers ( malondialdehyde, MDA and total thiols) from kidney homogenates were measured by spectrophotometric methods. Hematoxylin-eosin stain histopathologic examination of kidneys was performed. The obesogenic diet increased the serum levels for triglycerides (p[0.001) and renal function markers: urea (p[0.02), creatinine (p[0.02) and cystatin (p[0.01). The treatment reduced the kidney inflammation, prevented tubulonephrosis damage and improved the renal oxidative stress markers (p[0.05) in the high fat diet group. In conclusion, PLGA-NPs loaded with vitamin E had renoprotective effect in high fat diet Wistar rats.

scholarly journals Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet

2014 ◽  
Vol 6 (1) ◽  
pp. 130 ◽  
Author(s):  
Nathalie Auberval ◽  
Stéphanie Dal ◽  
William Bietiger ◽  
Michel Pinget ◽  
Nathalie Jeandidier ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Stress Markers ◽  
And Oxidative Stress

Effect of a high sucrose and high fat diet in BDNF (+/-) mice on oxidative stress markers in adipose tissues

2019 ◽  
Vol 665 ◽  
pp. 46-56 ◽  
Author(s):  
Akın Bodur ◽  
İmran İnce ◽  
Cemil Kahraman ◽  
İsmail Abidin ◽  
Selcen Aydin-Abidin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Adipose Tissues ◽  
Stress Markers ◽  
High Sucrose

scholarly journals Effects of Vitamin D and Simvastatin on Inflammatory and Oxidative Stress Markers of High-Fat Diet-Induced Obese Rats

2021 ◽  
Vol 2 (3) ◽  
pp. 39-50
Author(s):  
Abdel-Moniem A. Makhlouf ◽  
Atef M. Mahmoud ◽  
Rania G. Ibrahim ◽  
Yasmeen S. Abdel Aziz
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
High Fat Diet ◽  
Male Rats ◽  
Antioxidant Defenses ◽  
Control Group ◽  
High Fat ◽  
Stress Markers ◽  
Obese Rats ◽  
Markers Of Oxidative Stress

Purpose: This study was aimed to evaluate the antioxidant and anti-inflammatory effects of vitamin D and Simvastatin (SIM) on a high-fat diet (HFD) induced-obese rats. Methods: 40 adult male rats were divided into four groups: control group, HFD, HFD + vitamin D, and HFD + SIM for 14 weeks. Vitamin D or SIM supplementation was done for the last 6 weeks. Vitamin D dosage was 500 IU/kg, while SIM dosage was 10 mg/kg. Interleukin-6 (IL-6) concentration and markers of oxidative stress including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione(GSH) concentrations in serum were determined using ELISA kits and spectrophotometry methods, respectively. Results: Treatment with vitamin D or SIM could significantly reduce IL-6 and MDA and increases SOD, GPx activities, and GSH levels. Oxidative stress can result not only from increased ROS production but also from dysfunctional antioxidant defenses. Conclusion: From the experimental results, it was observed that SIM and vitamin D could attenuate oxidative stress and inflammation markers associated with obesity.

scholarly journals The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 183 ◽  
Author(s):  
Bronisława Skrzep-Poloczek ◽  
Jakub Poloczek ◽  
Elżbieta Chełmecka ◽  
Agnieszka Dulska ◽  
Ewa Romuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bariatric Surgery ◽  
Heart Muscle ◽  
High Fat Diet ◽  
Glutathione Transferase ◽  
Control Diet ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Stress Markers ◽  
Gpx Activity

Obesity and high-fat diet (HF) are prevalent causes of oxidative stress (OS). Duodenal-jejunal omega switch (DJOS) is a bariatric procedure used for body mass reduction, extensively tested in animal models. We studied the long-term impact of bariatric surgery and an HF diet on the oxidative stress markers in erythrocytes and heart muscles of rats. We analyzed superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) activity and malondialdehyde (MDA) concentration in DJOS or SHAM (control) operated rats fed with different dietary protocols (control diet (CD) and high-fat diet (HF)), before and after the surgery (CD/CD, HF/HF, CD/HF, and HF/CD). We observed higher erythrocytes CAT, GST and GPx activity in DJOS-operated (vs. SHAM) rats fed with an HF/HF diet. For DJOS-operated rats, erythrocytes CAT and GPx activity and MDA concentration were significantly lower in CD/CD group. We observed increased heart muscle GR activity in SHAM-operated rats (vs. DJOS bariatric surgery) fed with an HF/HF diet. Change from HF to CD diet increased heart muscle GPx activity after DJOS bariatric surgery. Heart muscle SOD activity was lower in HF/HF and CD/CD groups after DJOS bariatric surgery (vs. SHAM). DJOS surgery significantly reduced heart muscle MDA concentration in HF/HF and HF/CD groups (vs. SHAM). We conclude that the selected dietary patterns had a stronger impact on oxidative stress markers in erythrocytes and heart muscle than DJOS bariatric surgery.

scholarly journals ACE Reduces Metabolic Abnormalities in a High-Fat Diet Mouse Model

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Seong-Jong Lee ◽  
Jong-Min Han ◽  
Jin-Seok Lee ◽  
Chang-Gue Son ◽  
Hwi-Jin Im ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Serum Lipid ◽  
Peroxisome Proliferator ◽  
Metabolic Abnormalities ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Peroxisome Proliferator Activated Receptor ◽  
Stress Markers ◽  
And Oxidative Stress

The medicinal plantsArtemisia iwayomogi(A. iwayomogi) andCurcuma longa(C. longa) radix have been used to treat metabolic abnormalities in traditional Korean medicine and traditional Chinese medicine (TKM and TCM). In this study we evaluated the effect of the water extract of a mixture ofA. iwayomogiandC. longa(ACE) on high-fat diet-induced metabolic syndrome in a mouse model. Four groups of C57BL/6N male mice (except for the naive group) were fed a high-fat diet freely for 10 weeks. Among these, three groups (except the control group) were administered a high-fat diet supplemented with ACE (100 or 200 mg/kg) or curcumin (50 mg/kg). Body weight, accumulation of adipose tissues in abdomen and size of adipocytes, serum lipid profiles, hepatic steatosis, and oxidative stress markers were analyzed. ACE significantly reduced the body and peritoneal adipose tissue weights, serum lipid profiles (total cholesterol and triglycerides), glucose levels, hepatic lipid accumulation, and oxidative stress markers. ACE normalized lipid synthesis-associated gene expressions (peroxisome proliferator-activated receptor gamma, PPARγ; fatty acid synthase, FAS; sterol regulatory element-binding transcription factor-1c, SREBP-1c; and peroxisome proliferator-activated receptor alpha, PPARα). The results from this study suggest that ACE has the pharmaceutical potential reducing the metabolic abnormalities in an animal model.

Effect of Vitamin E Supplementation on Oxidative Stress in a Rat Model of Diet-induced Obesity

2009 ◽  
Vol 79 (4) ◽  
pp. 255-263 ◽  
Author(s):  
XiuHua Shen ◽  
QingYa Tang ◽  
Jiang Wu ◽  
Yi Feng ◽  
Juan Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Glucose Metabolism ◽  
Plasma Glucose ◽  
High Fat Diet ◽  
Intervention Group ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Diet Induced Obesity

Objective: To evaluate the effect of vitamin E on the level of oxidative stress in diet-induced obese Sprague-Dawley rats. Methods: Thirty weaning male rats were placed into three groups with 10 animals each: a control group with normal chow, a diet-induced obesity group (DIO) with high-fat diet, and an intervention group with high-fat diet supplemented with vitamin E (VE, 350 mg/kg). Blood and adipose tissue were collected from rats after 10 weeks. Biomarkers of oxidative stress were detected for plasma 8-epi-prostaglandin- F2α (8-epi-PGF2α), thiobarbituric acid-reactive substances (TBARS), total anti-oxidative capacity (TAOC), α-tocopherol, superoxide dismutase (SOD) activity, and glutathione peroxidase activity (GPx). Lipid and glucose metabolism parameters such as plasma glucose, insulin, and triacylglycerol (TG) were also analyzed. Results: After 10 weeks, all obese rats (both the DIO and VE groups) had higher plasma 8-epi-PGF2α and TBARS levels than the controls. Their plasma-adjusted α-tocopherol, SOD, and GPx activities were lower than the control levels but insulin was higher (p<0.01). The VE intervention increased plasma SOD, GPx, and T-AOC, and decreased 8-epi-PGF2α (p<0.05). VE intervention also decreased plasma glucose, insulin, and TG levels (p<0.05). Conclusions: Increased oxidative stress could be an important target for the prevention of obesity-related diseases. Vitamin E has moderate effects for improvement of oxidative stress status and glucose metabolism in the animal model of diet-induced obesity.

scholarly journals Effects of sesame oil on learning and memory impairment and biomarkers of oxidative stress in rats with a long-term high-fat diet consumption

2021 ◽  
Author(s):  
Mojtaba Rustaei ◽  
Reihaneh Sadeghian ◽  
Iraj Salehi ◽  
Abdolrahman Sarihi ◽  
Siamak Shahidi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Memory Impairment ◽  
Antioxidant Properties ◽  
Sesame Oil ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Positive Effects

Abstract Nowadays, high-fat foods are eaten in most societies, which causes memory impairment and anxiety through the oxidative stress pathway. Sesame oil (SO) has potential antioxidant properties. Therefore, the effects of sesame oil on memory impairment and anxiety caused by a high-fat diet (HFD) in male rats were investigated. Eighty male Wistar rats were divided into eight groups (n = 10): control (standard diet; SD), the HFD, SD + SO (0.5, 1, or 2 ml/kg; once/day, gavage), and HFD + SO (0.5, 1, or 2 ml/kg; once/day, gavage) groups. All diets were given to the animals for three months. Finally, behavioral and oxidative stress parameters were measured. The step-through latency of retention test in SD + SO (0.5 or 1 ml/kg) groups increased more than the control group. Also, the Barnes test on training days revealed that the latency time to find the target hole increased in the HFD group compared with the control group. Moreover, the time spent on the open arms in the SD + SO (0.5 ml/kg) group improved remarkably than the control group. Total oxidant (TOS) level in the HFD + SO (0.5, 1, and 2 ml/kg) groups was lower than the HFD group. The level of total antioxidant capacity (TAC) in the SD + SO (2 ml/kg) group was higher than the SD + SO (0.5 ml/kg) group and the amount of thiol in the HFD group decreased compared with the control group. These findings suggest that the positive effects of SO on memory and anxiety are probably due to its antioxidant properties and the elimination of free radicals.

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