Purified Pyocyanin from Clinical Isolates of Pseudomonas aeruginosa Enhances Antibiotic Sensitivity Against Some Pathogenic Bacteria

Pseudomonas aeruginosais a very important bacteria for public health because it is present in the environment and clinical infections. The aim of this study was toevaluate the virulence factors such as motility, protease and rhamnolipids in clinical and environmental P. aeruginosaisolates.Twenty-five clinical isolates and ten environmental isolates were analyzed by phenotypic assays and categorized into non-mobile, weakly, moderately and highly mobile strains; and producers of protease and rhamnolipids. The isolates were tested in triplicate on three different days. Environmental isolates produced virulence factors such as motility (Swimming and Twitching), and Ramnolipids significantly higher than clinical isolates.This study alerts us to the high level of pathogenicity of P. aeruginosastrains, mainly environmental strains. For a better understanding of motility and rhamnolipids, virulence factors that are directly associated with the biofilms formation, may favor studies that complement the research aimed at the control of pathogenic bacteria.

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Isolation and Diagnosis of Pathogenic Bacteria from the Upper Surface of the Cell Phone Screen and Conduct an Antibiotic Sensitivity Test

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.17 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Muthana Badeea Farhan ◽

Khadija Khleaf Abdulla

Keyword(s):

Pseudomonas Aeruginosa ◽

Cell Phone ◽

Pathogenic Bacteria ◽

Bacterial Culture ◽

Culture Media ◽

Antibiotic Sensitivity ◽

Personal Communication ◽

Total Sample ◽

Final Diagnosis ◽

Positive Bacterial Culture

A total of 120 samples (80) female samples and 40 male samples were collected from the front of the personal communication (the cell phone) for female students of Education College for Women (65) as well the medical staff of Ramadi Teaching Hospital (55), The samples are collected by swabs, The ages ranged between 19-60 years, These samples were initially culture on the blood agar medium and then on different selective and differential culture media. Api test strips were also used for the final diagnosis of bacterial isolates. The results showed that all samples gave 100% a positive result of general bacterial culture and 80% of pathogenic bacteria. The results of isolating and diagnosism indicated the presence of the pathogenic bacteria Staphylococcus aureus (58.3% of the total sample), Escherichia coli (44.2%), Enterococcus faecalis (25.8%), Pseudomonas aeruginosa (23.3%), Proteus sp. (5%), The results showed that the positive bacterial culture rate for male samples was 87.5%, whereas the female bacterial culture rate was 76.3%. The most important isolates were highly resistant to cephalexin, followed kanamycin. Proteus sp. gave the largest percentage of the resistance to 100% of those antibiotics, except for nitrofurantoin followed by Pseudomonas aeruginosa in the proportion of resistance to antibiotics used in this study.

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Antimicrobial Resistance of Pseudomonas Aeruginosa Isolated From Patients With Wound Infection in Ethiopia. A Systematic Review Article

10.21203/rs.3.rs-35693/v1 ◽

2020 ◽

Author(s):

Gebremichael Gebreegziabher ◽

Gebrehiwet Tesfahuneygn ◽

Aderajew Gebrewahd ◽

Abraham Aregay

Keyword(s):

Systematic Review ◽

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Wound Infection ◽

Clavulanic Acid ◽

Pathogenic Bacteria ◽

Clinical Isolates ◽

Local Data ◽

Amoxicillin Clavulanic Acid

Abstract Background: Infectious diseases have been major cause of morbidity and mortality all over the globe. The ability of bacterial pathogens to adapt and overcoming to treat by different antibiotics has been challenging in patient management. The antimicrobial resistance rates of Pseudomonas aeruginosa are known to fluctuate extensively in different settings. Active inspection of trends in antibiotic resistance of Pseudomonas aeruginosa is essential for the selection of suitable antimicrobial agent for empirical therapy. The objective of our systematic review was to determine the national antimicrobial resistance profile of Pseudomonas aeruginosa isolated from patients with wound infection in Ethiopia. Methods: We searched the Pub Med database in July and August 2018. We used the term ‘antimicrobial resistance, Pseudomonas aeruginosa, wound and Ethiopia’ to find articles published from 2011 to 2018 September. Only articles in English language were included. Full-text articles were incorporated if they reported the percentage of antibiotic resistance among clinical isolates of pathogenic bacteria collected from patients in any of the regions of Ethiopia. For overlapping studies reporting on the same clinical isolates, same study period and place, only the study with the largest sample size was included.Results: From the 173 Pseudomonas aeruginosa isolates 99.5%, 95% of the isolates were resistant to ampicilin and Amoxicillin Clavulanic Acid respectively. From the isolates 28.8%, 29.7% and 39.6% of them were resistant to Gentamicine, ciprofloxacin and Ceftazidime respectively. Most of the isolates have limited susceptibility to ampicillin, Amoxicillin Clavulanic Acid, tetracycline, cotromoxazole, chloramphenicol and ceftriaxone.Conclusion: Antimicrobial resistance is likely to become a challenge in Ethiopia and may be exacerbated by overuse of antibiotics, the lack of oversight of antibiotic prescription, and the lack of relevant local data on antimicrobial resistance. Therefore, existing antimicrobial stewardship programmes should be strengthened or, where they are not yet in place, they should be developed and implemented in all regional referral hospitals in response to these challenges and Health research institutes. Antimicrobial surveillance also needs to be strengthened.

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Evaluation of Bacterial Species in Patients with Skin Infection and their Antibiogram

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2021/v9i430214 ◽

2021 ◽

pp. 10-16

Author(s):

Immaculata U. Nwankwo ◽

Kelechi C. Edward ◽

Chinedu N. Nwoba ◽

Chinyere V. Okwudiri

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Pathogenic Bacteria ◽

Skin Infection ◽

Bacterial Species ◽

Antibiotic Sensitivity ◽

Percentage Occurrence ◽

Antibiotic Sensitivity Test ◽

Microbiological Criteria

Introduction: The skin is a barrier that limits invasion and growth of pathogenic bacteria. Bacterial species in patients with skin infection and their antibiogram were evaluated for the presence of inhabiting bacteria. Method: The sample obtained with swab sticks were streaked on their respective culture plates containing nutrient agar, MacConkey agar and blood agar, incubated 370 for 24h. The recovered bacteria after incubation were characterized and identified according to standard microbiological criteria. Results: Five bacteria species were isolated which includes Staphylococus aureus; Escherichia coli, Klebsiella specie, Proteus sp and Pseudomonas aeruginosa. Staphylococus aureus (33.03%) had the highest percentage occurrence while Proteus Sp (9.17%) had the lowest percentage occurrence. The highest number of isolates were recovered from male patients (61.47%). Age wise the least percentage of isolates were gotten from age group 31-40 (1.83%) while the highest was obtained from those within the age range of 11-20 (40.37%) followed by 1-10 years of age (25.69%). More isolates were recovered from in-patients (68.81%) than from Out-patients (31.19%). The antibiotic sensitivity test showed that Klebsiella sp, Proteus spp and Staphylococcus aureus were sensitivity to all the antibiotics tested while pseudomonas aeruginosa had 30% activity (sensitivity to only 3 drugs) for the antibiotics used in this study. Conclusion: From this study, it can be said that Staphylococcus aureus, Escherichia coli, Klebsiella sp, Proteus sp and Pseudomonas aeruginosa are implicated in the skin infections in humans and their presence if not treated will lead to long term disease in human affected.

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Effect of Tyrosol and Farnesol on Virulence and Antibiotic Resistance of Clinical Isolates ofPseudomonas aeruginosa

BioMed Research International ◽

10.1155/2015/456463 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 9

Author(s):

Shaymaa Hassan Abdel-Rhman ◽

Areej Mostafa El-Mahdy ◽

Mohammed El-Mowafy

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Candida Albicans ◽

Bacterial Pathogen ◽

Antibiotic Sensitivity ◽

Clinical Isolates ◽

Mixed Species ◽

Lower Extent ◽

Fungal Interactions ◽

First Time

Mixed-species biofilms could create a protected environment that allows for survival to external antimicrobials and allows different bacterial-fungal interactions.Pseudomonas aeruginosa-Candida albicanscoexistence is an example for such mixed-species community. Numerous reports demonstrated howP. aeruginosaor its metabolites could influence the growth, morphogenesis, and virulence ofC. albicans. In this study, we investigated how theC. albicansquorum sensing compounds, tyrosol and farnesol, might affect Egyptian clinical isolates ofP. aeruginosaregarding growth, antibiotic sensitivity, and virulence. We could demonstrate that tyrosol possesses an antibacterial activity againstP. aeruginosa(10 µM inhibited more than 50% of growth after 16 h cultivation). Moreover, we could show for the first time that tyrosol strongly inhibits the production of the virulence factors hemolysin and protease inP. aeruginosa, whereas farnesol inhibits, to lower extent, hemolysin production in this bacterial pathogen. Cumulatively, tyrosol is expected to strongly affectP. aeruginosain mixed microbial biofilm.

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Microbiological quality of residues from drinking water preparation

Water Science & Technology ◽

10.2166/wst.1995.0565 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 75-79 ◽

Cited By ~ 5

Author(s):

M. Würzer ◽

A. Wiedenmann ◽

K. Botzenhart

Keyword(s):

Waste Water ◽

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Drinking Water ◽

Water Treatment ◽

Pathogenic Bacteria ◽

Waste Water Treatment ◽

Microbiological Quality ◽

Detection Limits

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.

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In vitro synergistic effect of the CM11 antimicrobial peptide in combination with common antibiotics against clinical isolates of six species of multidrug-resistant pathogenic bacteria

Protein and Peptide Letters ◽

10.2174/0929866522666150728115439 ◽

2015 ◽

Vol 22 (10) ◽

pp. 940-951 ◽

Cited By ~ 16

Author(s):

Jafar Amani ◽

Kamal Barjini ◽

Mehrdad Moghaddam ◽

Asadollah Asadi

Keyword(s):

Synergistic Effect ◽

Antimicrobial Peptide ◽

Pathogenic Bacteria ◽

Multidrug Resistant ◽

Clinical Isolates

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Cephalosporin nitric oxide-donor prodrug DEA-C3D disperses biofilms formed by clinical cystic fibrosis isolates of Pseudomonas aeruginosa

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz378 ◽

2019 ◽

Vol 75 (1) ◽

pp. 117-125 ◽

Cited By ~ 8

Author(s):

Odel Soren ◽

Ardeshir Rineh ◽

Diogo G Silva ◽

Yuming Cai ◽

Robert P Howlin ◽

...

Keyword(s):

Nitric Oxide ◽

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Clinical Isolates ◽

Nitric Oxide Donor ◽

Confocal Laser ◽

Broth Microdilution Method

Abstract Objectives The cephalosporin nitric oxide (NO)-donor prodrug DEA-C3D (‘DiEthylAmin-Cephalosporin-3′-Diazeniumdiolate’) has been shown to initiate the dispersal of biofilms formed by the Pseudomonas aeruginosa laboratory strain PAO1. In this study, we investigated whether DEA-C3D disperses biofilms formed by clinical cystic fibrosis (CF) isolates of P. aeruginosa and its effect in combination with two antipseudomonal antibiotics, tobramycin and colistin, in vitro. Methods β-Lactamase-triggered release of NO from DEA-C3D was confirmed using a gas-phase chemiluminescence detector. MICs for P. aeruginosa clinical isolates were determined using the broth microdilution method. A crystal violet staining technique and confocal laser scanning microscopy were used to evaluate the effects of DEA-C3D on P. aeruginosa biofilms alone and in combination with tobramycin and colistin. Results DEA-C3D was confirmed to selectively release NO in response to contact with bacterial β-lactamase. Despite lacking direct, cephalosporin/β-lactam-based antibacterial activity, DEA-C3D was able to disperse biofilms formed by three P. aeruginosa clinical isolates. Confocal microscopy revealed that DEA-C3D in combination with tobramycin produces similar reductions in biofilm to DEA-C3D alone, whereas the combination with colistin causes near complete eradication of P. aeruginosa biofilms in vitro. Conclusions DEA-C3D is effective in dispersing biofilms formed by multiple clinical isolates of P. aeruginosa and could hold promise as a new adjunctive therapy to patients with CF.

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Assessing the Molecular Targets and Mode of Action of Furanone C-30 on Pseudomonas aeruginosa Quorum Sensing

Molecules ◽

10.3390/molecules26061620 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1620

Author(s):

Victor Markus ◽

Karina Golberg ◽

Kerem Teralı ◽

Nazmi Ozer ◽

Esti Kramarsky-Winter ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Quorum Sensing ◽

Conformational Changes ◽

Mode Of Action ◽

Pathogenic Bacteria ◽

Molecular Targets ◽

Resistant Bacteria ◽

Public Healthcare ◽

C 30

Quorum sensing (QS), a sophisticated system of bacterial communication that depends on population density, is employed by many pathogenic bacteria to regulate virulence. In view of the current reality of antibiotic resistance, it is expected that interfering with QS can address bacterial pathogenicity without stimulating the incidence of resistance. Thus, harnessing QS inhibitors has been considered a promising approach to overriding bacterial infections and combating antibiotic resistance that has become a major threat to public healthcare around the globe. Pseudomonas aeruginosa is one of the most frequent multidrug-resistant bacteria that utilize QS to control virulence. Many natural compounds, including furanones, have demonstrated strong inhibitory effects on several pathogens via blocking or attenuating QS. While the natural furanones show no activity against P. aeruginosa, furanone C-30, a brominated derivative of natural furanone compounds, has been reported to be a potent inhibitor of the QS system of the notorious opportunistic pathogen. In the present study, we assess the molecular targets and mode of action of furanone C-30 on P. aeruginosa QS system. Our results suggest that furanone C-30 binds to LasR at the ligand-binding site but fails to establish interactions with the residues crucial for the protein’s productive conformational changes and folding, thus rendering the protein dysfunctional. We also show that furanone C-30 inhibits RhlR, independent of LasR, suggesting a complex mechanism for the agent beyond what is known to date.

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