ANTAGONISTIC EFFECT OF MONOCULTURES OF MILK-OXIDE BACTERIA AND THEIR CONSORTIUM WITH YEASTS ON PATHOGENIC BACTERIA AND FUNGI

2021 ◽  
Vol 1 (19) ◽  
pp. 365-367
Author(s):  
E.F. Semenova ◽  
L.M Teplitskaya ◽  
G.P. Zaitsev ◽  
I.E. Tsokalo ◽  
A.V. Omelchenko ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbial Consortium ◽  
Culture Fluid ◽  
Antagonistic Effect ◽  
Maximum Effect ◽  
Bacteria And Fungi

The efficiency of the consortium of lactic oxide bacteria and yeast is shown to suppress phytophotogenic bacteria and micromicets, conditionally pathogenic bacteria in vitro conditions. It has been shown that 1.0% of the solution of the culture fluid of the microbial consortium has the maximum effect in reducing the infestation of seed cereals.

scholarly journals Antimicrobial Activity of Lemongrass Essential Oil (Cymbopogon flexuosus) and Its Active Component Citral Against Dual-Species Biofilms of Staphylococcus aureus and Candida Species

2020 ◽  
Vol 10 ◽  
Author(s):  
Shanjun Gao ◽  
Guangzhi Liu ◽  
Jianguo Li ◽  
Jing Chen ◽  
Lina Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Expression Of Genes ◽  
Cymbopogon Flexuosus ◽  
Underlying Mechanisms ◽  
Bacteria And Fungi ◽  
Conventional Antibiotics ◽  
Lemongrass Essential Oil

Compared to mono-species biofilm, biofilms formed by cross-kingdom pathogens are more refractory to conventional antibiotics, thus complicating clinical treatment and causing significant morbidity. Lemongrass essential oil and its bioactive component citral were previously demonstrated to possess strong antimicrobial efficacy against pathogenic bacteria and fungi. However, their effects on polymicrobial biofilms remain to be determined. In this study, the efficacy of lemongrass (Cymbopogon flexuosus) essential oil and its bioactive part citral against dual-species biofilms formed by Staphylococcus aureus and Candida species was evaluated in vitro. Biofilm staining and viability test showed both lemongrass essential oil and citral were able to reduce biofilm biomass and cell viability of each species in the biofilm. Microscopic examinations showed these agents interfered with adhesive characteristics of each species and disrupted biofilm matrix through counteracting nucleic acids, proteins and carbohydrates in the biofilm. Moreover, transcriptional analyses indicated citral downregulated hyphal adhesins and virulent factors of Candida albicans, while also reducing expression of genes involved in quorum sensing, peptidoglycan and fatty acids biosynthesis of S. aureus. Taken together, our results demonstrate the potential of lemongrass essential oil and citral as promising agents against polymicrobial biofilms as well as the underlying mechanisms of their activity in this setting.

In vitro assessment of N-(benzyl)chitosan derivatives against some plant pathogenic bacteria and fungi

2009 ◽  
Vol 45 (1) ◽  
pp. 237-245 ◽  
Author(s):  
Entsar I. Rabea ◽  
Mohamed E.I. Badawy ◽  
Walter Steurbaut ◽  
Christian V. Stevens
Keyword(s):  
Pathogenic Bacteria ◽  
Chitosan Derivatives ◽  
Plant Pathogenic Bacteria ◽  
In Vitro Assessment ◽  
Bacteria And Fungi

A Survey of Enterobacteriaceae and Resistance of Greater Rhea (Rhea Americana) Eggs to Experimental Contamination by Salmonella Enteritidis

2017 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Renato Clini Cervi ◽  
Maria Auxiliadora Andrade ◽  
Cíntia Silva Minafra-Rezende ◽  
Marcos Barcellos Café
Keyword(s):  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Bird Species ◽  
Storage Period ◽  
Salmonella Spp ◽  
Rhea Americana ◽  
Greater Rhea ◽  
Bacteria And Fungi ◽  
Protective Structures

Eggs have protective structures against possible contamination by bacteria and fungi. Pathogenic bacteria such as Salmonella spp. are therefore rarely found in adequate sanitary conditions. Few studies about the contamination of ratite eggs have been reported, and although some studies have focused on ostrich eggs, there is no information about Greater Rhea ( Rhea americana) eggs. The microorganisms that contaminate ratite eggs do not differ from those that affect other bird species. The purpose of this study was to characterise contamination in 16 eggs, supplied by a commercial breeding facility, by Enterobacteriaceae contamination and to assess the in vitro resistance to experimental contamination by Salmonella Enteritidis using analytical tests following the guidelines of the Georgia Poultry Laboratory. These elements are critical to the storage period, quality and physicochemical parameters. The results revealed the presence of the genera Proteus, Klebsiella, Citrobacter and Enterobacter related to the location of nests. Greater Rhea eggs were also found to be highly resistant to experimental infection by Salmonella Enteritidis.

Compatibility of Commercially Produced Protective Cultures with Common Cheesemaking Cultures and Their Antagonistic Effect on Foodborne Pathogens

2020 ◽  
Vol 83 (6) ◽  
pp. 1010-1019 ◽  
Author(s):  
CATHERINE A. GENSLER ◽  
STEPHANIE R. B. BROWN ◽  
SULAIMAN F. ALJASIR ◽  
DENNIS J. D'AMICO
Keyword(s):  
Foodborne Pathogens ◽  
Pathogenic Bacteria ◽  
Antagonistic Effect ◽  
Starter Cultures ◽  
Minimal Impact ◽  
Cheese Industry ◽  
Ultrahigh Temperature ◽  
Bacteria And Fungi ◽  
The Impact ◽  
Potential Use

ABSTRACT The documented survival of pathogenic bacteria, including Listeria monocytogenes (LM), Shiga toxin–producing Escherichia coli (STEC), and Salmonella during the manufacture and aging of some cheeses highlights the need for additional interventions to enhance food safety. Unfortunately, few interventions are compliant with the Standards of Identity for cheese. Protective bacterial cultures (PCs) represent actionable, natural interventions. However, supportive data for commercially produced PCs regarding their efficacy against pathogens and potential antagonism with each other and cheesemaking cultures are scant, thereby impeding their potential use by the cheese industry. The overall objective of this study was to identify commercially produced PCs that exert antimicrobial activity toward pathogens with minimal impact on beneficial cheese microbes. Direct antagonism and agar well diffusion assays were used to determine the impact of 10 commercially produced PCs on the growth of starter cultures and cultures of ripening bacteria and fungi. Deferred antagonism was used to evaluate the potential for antimicrobial effects against LM, STEC, and Salmonella. PCs and starter cultures were cocultured in ultrahigh-temperature-processed milk to determine the effects of coculture on starter acidification profiles when incubated according to a simulated cheesemaking temperature profile (4 h at 35°C followed by 20 h at 20°C). Compatibility assays suggest that PC antagonism is microbe and strain specific. Only one PC negatively impacted the acidification of the starters tested. PC antagonism of ripening bacteria and fungi growth varied but was consistent within species. All PCs displayed deferred inhibition of LM, STEC, and Salmonella growth, but to varying degrees. These data identify commercial PCs with potential for the control of pathogens and characterize their compatibility with cheesemaking cultures for future use by cheesemakers and investigations of their efficacy in the production of cheese. HIGHLIGHTS

scholarly journals Impact of Moringa aqueous extract on pathogenic bacteria and fungi in vitro

2016 ◽  
Vol 61 (2) ◽  
pp. 247-250 ◽  
Author(s):  
Latifa A. Al_husnan ◽  
Muneera D.F. Alkahtani
Keyword(s):  
Aqueous Extract ◽  
Pathogenic Bacteria ◽  
Bacteria And Fungi

scholarly journals Transition Metal Complexes of Mixed Bioligands: Synthesis, Characterization, DFT Modeling, and Applications

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Mohamed S. A. Abdel-Mottaleb ◽  
Eman H. Ismail
Keyword(s):  
Transition Metal ◽  
Metal Complexes ◽  
Corrosion Inhibition ◽  
Transition Metal Complexes ◽  
Pathogenic Bacteria ◽  
Chemical Potential ◽  
Biological Activities ◽  
Aluminum Surface ◽  
Bacteria And Fungi

Divalent transition metal complexes [MGlu-Arg (H2O)]H2O and [MGlu-Arg (H2O)]H2O, where M = Co, Ni, Cu, and Zn, Glu = glutamic acid, and Arg = L-arginine, are prepared and characterized using different techniques. DFT and TD-DFT modelling validated and interpreted some experimental results. Weight loss technique reveals efficient corrosion inhibition action of these complexes towards aluminum metal at different temperatures. Our results point to corrosion inhibition through chemical adsorption on the aluminum surface. Additionally, a facile calcination of Co and Cu complexes at 550°C yields nanosized oxides of Co3O4, CoO, and CuO crystalline phases. The complexes show remarkable biological activities towards pathogenic bacteria and fungi. Moreover, in vitro anticancer activity evaluation of these complexes is achieved against hepatocellular carcinoma (HepG-2). The results are correlated with molecular descriptors such as chemical potential and hardness obtained from the frontier orbitals.

In-Vitro Cytotoxicity, Antimicrobial activities and DNA binding studies, of Novel Copper (II) complex with Heterocyclic base

2021 ◽  
pp. 3051-3055
Author(s):  
Shalini V. ◽  
Deepika R. ◽  
Arumugham M. N
Keyword(s):  
Dna Binding ◽  
Pathogenic Bacteria ◽  
Spectroscopic Method ◽  
Antimicrobial Activities ◽  
In Vitro Cytotoxicity ◽  
Binding Studies ◽  
Heterocyclic Base ◽  
Heterocyclic Ligand ◽  
Bacteria And Fungi

A novel ternary copper (II) complex have been synthesized by the addition of N, N-heterocyclic ligand with L-amino acid. The copper (II) complex, [Cu(1,10-phen) (L-Thr) Br] (where phen=1,10-phenanthroline and Threo=Threonine) characterized by various spectroscopic method. The intense UV band around 271nm was due to π-π* transition. The DNA binding study of these copper (II) complex are examined by UV–Visible, Emission spectroscopic, cyclic voltammetric and viscosity method. The results revealed that complex shown to be a intercalation mode of binding into DNA. The anticancer activity of Cu (II) complexes has capability to the kill HepG2 liver cancer cell as assessed by the MTT method, The Ic50value was found 21.50µg/ml The biological activity of the complex tested against certain pathogenic bacteria and fungi results revealed it was found to be potent antibacterial agent.

scholarly journals Novel Synthetic Strategy of Cyclic Dithiocarbamates Catalyzed by Triton-B

2019 ◽  
Vol 31 (5) ◽  
pp. 1091-1094 ◽  
Author(s):  
R. Kishore ◽  
M. Kamboj ◽  
M. Shukla ◽  
N. Srivastava
Keyword(s):  
Pathogenic Bacteria ◽  
Phase Transfer ◽  
Antimicrobial Activities ◽  
Spectroscopic Methods ◽  
Mild Conditions ◽  
Synthetic Strategy ◽  
Green Approach ◽  
Bacteria And Fungi ◽  
Ethyl Bromopyruvate

A simple, rapid and green methodology to synthesize cyclic dithiocarbamates (compounds 1-9) was developed by the reaction of 1° amines, CS2 and ethyl 3-bromo-2-oxopropanoate (ethyl bromopyruvate) facilitated by Triton-B as phase transfer catalyst. These compounds (1-9) were characterized with the help of elemental analysis, IR, NMR and mass spectroscopic methods This efficient green approach requires mild conditions and gives good yield of product. In vitro antimicrobial activities of these compounds are reported against the pathogenic bacteria and fungi.

scholarly journals Antimicrobial activities of isolated probiotics and their metabolites against some pathogenic microorganisms

IIUC Studies ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 21-28
Author(s):  
Mohammed Abu Sayeed ◽  
Altaf Hossen ◽  
Repon Saha ◽  
Md Jakaria
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Antibacterial Property ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Maximum Effect ◽  
Zone Of Inhibition ◽  
Agar Media ◽  
Bacteria And Fungi

The study was aimed to finding the antimicrobial activities among probiotics isolated from different yoghurts and their metabolites against some common bacterial pathogens. The nutrient agar media overlay method (Disc diffusion Method) was used to determine the presence of antibacterial effects among the isolated probiotics. Probiotics produced potential antibacterial activities against several pathogenic bacteria and fungi. The maximum antibacterial property (13.5 mm of zone of inhibition) of bacterial strain found against Salmonella paratyphi. Conversly, bacterial metabolites produced maximum effect (10.3 mm of zone of inhibition) against Staphylocuccos aureaus. The antibacterial effect is one of the most important criteria for probiotics selection, and the verified antibacterial activities of the probiotics supports the development of these functional foods as a key to the enhancement of health in the consuming public.IIUC Studies Vol.14(1) June 2017: 21-28

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