Novel Synthetic Strategy of Cyclic Dithiocarbamates Catalyzed by Triton-B

A simple, rapid and green methodology to synthesize cyclic dithiocarbamates (compounds 1-9) was developed by the reaction of 1° amines, CS2 and ethyl 3-bromo-2-oxopropanoate (ethyl bromopyruvate) facilitated by Triton-B as phase transfer catalyst. These compounds (1-9) were characterized with the help of elemental analysis, IR, NMR and mass spectroscopic methods This efficient green approach requires mild conditions and gives good yield of product. In vitro antimicrobial activities of these compounds are reported against the pathogenic bacteria and fungi.

Download Full-text

In-Vitro Cytotoxicity, Antimicrobial activities and DNA binding studies, of Novel Copper (II) complex with Heterocyclic base

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00533 ◽

2021 ◽

pp. 3051-3055

Author(s):

Shalini V. ◽

Deepika R. ◽

Arumugham M. N

Keyword(s):

Dna Binding ◽

Pathogenic Bacteria ◽

Spectroscopic Method ◽

Antimicrobial Activities ◽

In Vitro Cytotoxicity ◽

Binding Studies ◽

Heterocyclic Base ◽

Heterocyclic Ligand ◽

Bacteria And Fungi

A novel ternary copper (II) complex have been synthesized by the addition of N, N-heterocyclic ligand with L-amino acid. The copper (II) complex, [Cu(1,10-phen) (L-Thr) Br] (where phen=1,10-phenanthroline and Threo=Threonine) characterized by various spectroscopic method. The intense UV band around 271nm was due to π-π* transition. The DNA binding study of these copper (II) complex are examined by UV–Visible, Emission spectroscopic, cyclic voltammetric and viscosity method. The results revealed that complex shown to be a intercalation mode of binding into DNA. The anticancer activity of Cu (II) complexes has capability to the kill HepG2 liver cancer cell as assessed by the MTT method, The Ic50value was found 21.50µg/ml The biological activity of the complex tested against certain pathogenic bacteria and fungi results revealed it was found to be potent antibacterial agent.

Download Full-text

Antimicrobial Activities of Marine Sponge-Associated Bacteria

Microorganisms ◽

10.3390/microorganisms9010171 ◽

2021 ◽

Vol 9 (1) ◽

pp. 171

Author(s):

Yitayal S. Anteneh ◽

Qi Yang ◽

Melissa H. Brown ◽

Christopher M. M. Franco

Keyword(s):

Pathogenic Bacteria ◽

South Australia ◽

Multidrug Resistant ◽

Antimicrobial Activities ◽

Marine Sponges ◽

Bioactive Metabolites ◽

Human Pathogens ◽

Sponge Associated Bacteria ◽

Microbial Symbionts ◽

Bacteria And Fungi

The misuse and overuse of antibiotics have led to the emergence of multidrug-resistant microorganisms, which decreases the chance of treating those infected with existing antibiotics. This resistance calls for the search of new antimicrobials from prolific producers of novel natural products including marine sponges. Many of the novel active compounds reported from sponges have originated from their microbial symbionts. Therefore, this study aims to screen for bioactive metabolites from bacteria isolated from sponges. Twelve sponge samples were collected from South Australian marine environments and grown on seven isolation media under four incubation conditions; a total of 1234 bacterial isolates were obtained. Of these, 169 bacteria were tested in media optimized for production of antimicrobial metabolites and screened against eleven human pathogens. Seventy bacteria were found to be active against at least one test bacterial or fungal pathogen, while 37% of the tested bacteria showed activity against Staphylococcus aureus including methicillin-resistant strains and antifungal activity was produced by 21% the isolates. A potential novel active compound was purified possessing inhibitory activity against S. aureus. Using 16S rRNA, the strain was identified as Streptomyces sp. Our study highlights that the marine sponges of South Australia are a rich source of abundant and diverse bacteria producing metabolites with antimicrobial activities against human pathogenic bacteria and fungi.

Download Full-text

Antimicrobial activities of an oxygenated cyclohexanone derivative isolated from Amphirosellinia nigrospora JS-1675 against various plant pathogenic bacteria and fungi

Journal of Applied Microbiology ◽

10.1111/jam.14138 ◽

2019 ◽

Vol 126 (3) ◽

pp. 894-904 ◽

Cited By ~ 5

Author(s):

H.T. Nguyen ◽

S. Kim ◽

N.H. Yu ◽

A.R. Park ◽

H. Yoon ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Antimicrobial Activities ◽

Plant Pathogenic Bacteria ◽

Bacteria And Fungi ◽

Cyclohexanone Derivative

Download Full-text

DEVELOPMENT OF IN VITRO METHODOLOGIES FOR INHIBITION OF PATHOGENIC BACTERIA BY POTENTIAL PROBIOTIC LACTOBACILLUS SPS; AN EVIDENCE FOR PRODUCTION OF ANTIMICROBIAL SUBSTANCES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14894 ◽

2016 ◽

Vol 8 (12) ◽

pp. 277

Author(s):

Prabhurajeshwar C. ◽

Kelmani Chandrakanth R.

Keyword(s):

Bile Salts ◽

Pathogenic Bacteria ◽

Antimicrobial Activities ◽

Local Market ◽

Preliminary Screening ◽

Milk Products ◽

Antimicrobial Substances ◽

Live Bacteria ◽

Digestive Health

Objective: Probiotic products consist of specific strains of live bacteria that have potentially favorable health effects. A number of studies provide evidence that milk products with probiotics may be beneficial for digestive health and may improve various digestive problems. The purpose of the present study was to investigate Lactobacillus species with potential activities isolated from different cheese samples of local market.Methods: A total 42 lactic acid bacteria strains were isolated, fourteen (14/42) best Lactobacillus isolates were selected by preliminary screening as potential probiotics with antimicrobial activity against pathogenic bacteria. All the fourteen Lactobacillus isolates were then characterized in vitro for their probiotic features and antimicrobial activities against pathogens.Results: The results noticed that all selected Lactobacillus isolates (CH3, CH4 and CH6) were screened and confirmed as Lactobacillus. The isolates were able to grow at different pH, NaCl and bile salts, also exhibited the best antimicrobial activities against pathogens. All the isolates were susceptible to antibiotics used and isolates were also revealed the noticeable aggregation and hydrophobicity studies.Conclusion: Selected Lactobacillus isolates were considered as ideal, effective probiotic bacteria. Thus, they could be examined further and contribute to preventing and controlling several infections associated with intestine and for human health benefits.

Download Full-text

Biosurfactants Induce Antimicrobial Peptide Production through the Activation of TmSpatzles in Tenebrio molitor

International Journal of Molecular Sciences ◽

10.3390/ijms21176090 ◽

2020 ◽

Vol 21 (17) ◽

pp. 6090

Author(s):

Tariku Tesfaye Edosa ◽

Yong Hun Jo ◽

Maryam Keshavarz ◽

In Seon Kim ◽

Yeon Soo Han

Keyword(s):

Antimicrobial Peptide ◽

Immune Activation ◽

Tenebrio Molitor ◽

Antimicrobial Activities ◽

Iturin A ◽

E Coli ◽

Fat Bodies ◽

Escherichia Coli Bacteria ◽

Bacteria And Fungi

Biosurfactant immunomodulatory activities in mammals, nematodes, and plants have been investigated. However, the immune activation property of biosurfactants in insects has not been reported. Therefore, here, we studied the defense response triggered by lipopeptides (fengycin and iturin A), glycolipids (rhamnolipid), and cyclic polypeptides (bacitracin) in the coleopteran insect, mealworm Tenebrio molitor. The in vitro antimicrobial activities against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and fungi (Candida albicans) were assessed by mixing these pathogens with the hemolymph of biosurfactant-immune-activated larvae. E. coli growth was remarkably inhibited by this hemolymph. The antimicrobial peptide (AMP) induction results also revealed that all biosurfactants tested induced several AMPs, exclusively in hemocytes. The survivability analysis of T. molitor larvae challenged by E. coli (106 CFU/µL) at 24 h post biosurfactant-immune activation showed that fengycin, iturin A, and rhamnopid significantly increased survivability against E. coli. Biosurfactant-induced TmSpatzles activation was also monitored, and the results showed that TmSpz3 and TmSpz-like were upregulated in the hemocytes of iturin A-injected larvae, while TmSpz4 and TmSpz6 were upregulated in the fat bodies of the fengycin-, iturin A-, and rhamnolipid-injected larvae. Overall, these results suggest that lipopeptide and glycolipid biosurfactants induce the expression of AMPs in T. molitor via the activation of spätzle genes, thereby increasing the survivability of T. molitor against E. coli.

Download Full-text

Antimicrobial Activity of Lemongrass Essential Oil (Cymbopogon flexuosus) and Its Active Component Citral Against Dual-Species Biofilms of Staphylococcus aureus and Candida Species

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.603858 ◽

2020 ◽

Vol 10 ◽

Author(s):

Shanjun Gao ◽

Guangzhi Liu ◽

Jianguo Li ◽

Jing Chen ◽

Lina Li ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Expression Of Genes ◽

Cymbopogon Flexuosus ◽

Underlying Mechanisms ◽

Bacteria And Fungi ◽

Conventional Antibiotics ◽

Lemongrass Essential Oil

Compared to mono-species biofilm, biofilms formed by cross-kingdom pathogens are more refractory to conventional antibiotics, thus complicating clinical treatment and causing significant morbidity. Lemongrass essential oil and its bioactive component citral were previously demonstrated to possess strong antimicrobial efficacy against pathogenic bacteria and fungi. However, their effects on polymicrobial biofilms remain to be determined. In this study, the efficacy of lemongrass (Cymbopogon flexuosus) essential oil and its bioactive part citral against dual-species biofilms formed by Staphylococcus aureus and Candida species was evaluated in vitro. Biofilm staining and viability test showed both lemongrass essential oil and citral were able to reduce biofilm biomass and cell viability of each species in the biofilm. Microscopic examinations showed these agents interfered with adhesive characteristics of each species and disrupted biofilm matrix through counteracting nucleic acids, proteins and carbohydrates in the biofilm. Moreover, transcriptional analyses indicated citral downregulated hyphal adhesins and virulent factors of Candida albicans, while also reducing expression of genes involved in quorum sensing, peptidoglycan and fatty acids biosynthesis of S. aureus. Taken together, our results demonstrate the potential of lemongrass essential oil and citral as promising agents against polymicrobial biofilms as well as the underlying mechanisms of their activity in this setting.

Download Full-text

Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

Czech Journal of Food Sciences ◽

10.17221/753-cjfs ◽

2008 ◽

Vol 25 (No. 2) ◽

pp. 81-89 ◽

Cited By ~ 43

Author(s):

A. Adiguzel ◽

H. Ozer ◽

H. Kilic ◽

B. Cetin

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Antimicrobial Activities ◽

Satureja Hortensis ◽

Bacteria And Fungi

The present work reports the in vitro antimicrobial activities of the essential oil and methanol extract from Satureja hortensis as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), γ-terpinene (18.56%), carvacrol (13.98%), and p-cymene (8.97). The essential oil of Satureja hortensis exhibited the activity against 25 bacteria, 8 fungi, and a yeast, C. albicans; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 µl/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

Download Full-text

In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

International Journal of Microbiology ◽

10.1155/2016/9545693 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 30

Author(s):

Awol Mekonnen ◽

Berhanu Yitayew ◽

Alemnesh Tesema ◽

Solomon Taddese

Keyword(s):

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Drug Preparation ◽

Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

Download Full-text

In vitro assessment of N-(benzyl)chitosan derivatives against some plant pathogenic bacteria and fungi

European Polymer Journal ◽

10.1016/j.eurpolymj.2008.10.021 ◽

2009 ◽

Vol 45 (1) ◽

pp. 237-245 ◽

Cited By ~ 63

Author(s):

Entsar I. Rabea ◽

Mohamed E.I. Badawy ◽

Walter Steurbaut ◽

Christian V. Stevens

Keyword(s):

Pathogenic Bacteria ◽

Chitosan Derivatives ◽

Plant Pathogenic Bacteria ◽

In Vitro Assessment ◽

Bacteria And Fungi

Download Full-text

Antimicrobial and Antioxidant Isoflavonoid Derivatives from the Roots of Amphimas pterocarpoides

Zeitschrift für Naturforschung B ◽

10.5560/znb.2013-3106 ◽

2013 ◽

Vol 68 (8) ◽

pp. 931-938 ◽

Cited By ~ 3

Author(s):

Edwige P. Fodja Saah ◽

Valerie Tedjon Sielinou ◽

Victor Kuete ◽

Stephen T. Lacmata ◽

Augustin E. Nkengfack

Keyword(s):

Antioxidant Properties ◽

2D Nmr ◽

Antimicrobial Activities ◽

Spectroscopic Methods ◽

Chemical Transformations ◽

Spectroscopic Evidence ◽

Wide Range ◽

Bacteria And Fungi

A new bis-isoflavone derivative, named amphiisoflavone (1), was isolated from the roots of Amphimas pterocarpoides, together with three known isoflavones, namely 8-methoxyisoformononetin (2), 6-methoxyisoformononetin (3) and isoformononetin (4). Chemical transformations carried out on compound 3 led to two new derivatives, 4´-acetoxy-6,7-dimethylisoflavone (3a) and 4´-O-prenyl- 6,7-dimethylisoflavone (3p), along with a known compound, 4´,6,7-trimethoxyisoflavone (3m). The structures of all compounds were elucidated from spectroscopic evidence, in particular by 1D and 2D NMR spectroscopic methods. These compounds were evaluated for their antioxidant properties and for their antimicrobial activities against a wide range of bacteria and fungi.

Download Full-text