scholarly journals In Vitro Characterization of Indigenous Coconut Varieties (Cocos nucifera L.) of Sri Lanka for Water Stress Tolerance

CORD ◽  
2012 ◽  
Vol 28 (2) ◽  
pp. 9
Author(s):  
L Perera
Keyword(s):  
Water Stress ◽  
Survival Rate ◽  
Sri Lanka ◽  
Drought Tolerance ◽  
Culture Medium ◽  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Water Stress Tolerance ◽  
Water Stress Condition

Coconuts show a remarkable yield drop due to long dry periods caused by global climatic changes. Thus, breeding for drought tolerance has become a priority in coconut breeding in Sri Lanka. Five coconut varieties indigenous to Sri Lanka, namely Ran thembili, Gon thembili, Porapol, Bodiri and Red dwarf were screened for their drought tolerance potential in vitro. Mature zygotic embryos collected from self pollinated nuts of each variety were germinated and developed into plants with one photosynthetic leaf in Y3 medium. Water stress condition was induced by application of Polyethylene Glycol (PEG) into the culture medium. The level of PEG in culture medium was gradually increased from 2% to 7% until the plants showed water stress symptoms in leaves; yellowing and necrosis. More than 75% of zygotic embryos of all tested coconut varieties successfully germinated in vitro and developed into plants. Plants of all tested coconut varieties survived water stress caused by 4% to 6% PEG and the percentages of plant survival at different levels of PEG varied among different coconut varieties. Variety Ran thembili showed the highest survival rate (27%) at 6% PEG and overall best plant growth performances at 4% PEG among the five coconut varieties tested. Red dwarf showed the lowest survival rate at 4% to 6% PEG indicating its susceptibility to water stress. Among the coconut varieties tested, zygotic embryos of Ran thembili showed the highest drought tolerance potential.

Antioxidant system response of different wheat cultivars under drought: field and in vitro studies

2001 ◽  
Vol 28 (11) ◽  
pp. 1095 ◽  
Author(s):  
Hernán R. Lascano ◽  
Gerardo E. Antonicelli ◽  
Celina M. Luna ◽  
Mariana N. Melchiorre ◽  
Leonardo D. Gómez ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Tolerance ◽  
Oxidative Damage ◽  
Stress Tolerance ◽  
Antioxidant System ◽  
System Response ◽  
Wheat Cultivars ◽  
System Activity ◽  
Water Stress Tolerance

The participation of the antioxidant system in the drought tolerance of wheat cultivars (Triticum aestivum L.) was studied under field and in vitro conditions. Under field conditions, drought tolerance was evaluated by the capacity to maintain the grain yield under drought, which was higher in cvv. Elite and La Paz than in the sensitive cvv. Oasis and Cruz Alta. Tolerant cultivars showed lower relative water content (RWC) and lower above-ground vegetative biomass than sensitive cultivars. Field assays did not show a clear correlation between water-stress tolerance and antioxidant system behaviour. However, when leaves of cvv. with contrasting drought tolerance were subjected to osmotic stress in vitro, clear differences in the antioxidant system activity and oxidative damage between cvv. were observed. In the tolerant cultivar Elite, it was possible to observe an increase in ascorbate peroxidase (APX), superoxide dismutase (SOD) and glutathione reductase (GR) activities, a higher glutathione (GSH) and ascorbate content and less oxidative damage than in the sensitive cultivar Oasis, which showed no changes or only slight decreases in the enzyme activities. These results indicate that water stress tolerance is in part associated with the antioxidant system activity, and suggest that the behaviour of the antioxidant systemin vitro assays can be used as an early selection tool.

scholarly journals The Growth Response of Several Potato Genotypes (Solanum tuberosum L.) to Induced Water Stress Using Sorbitol and Polyethylene Glycol

2016 ◽  
Vol 8 (4) ◽  
pp. 511-519 ◽  
Author(s):  
Mihaela A. CIOLOCA ◽  
Andreea M. TICAN ◽  
Maria IANOŞI ◽  
Carmen L. BĂDĂRĂU
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Drought Tolerance ◽  
Growth Response ◽  
Culture Medium ◽  
Growth Parameters ◽  
The Other ◽  
Addition Lines ◽  
In Vitro Response

The current paper aimed to study the in vitro response of potato genotypes to water stress induced by adding sorbitol and polyethylene glycol in the culture medium. The biological material analysed in the experiment was represented by a Romanian line ‘LP 11-1525/1’ and two isogenic lines ‘LI 101’ and ‘LI 102’. For cultures initiation, the line ‘LP 11-1525/1’ was started from meristems and for the other two genotypes true potato seeds were used. The studied potato genotypes behaved differently depending on the analysed parameters and on the treatment applied for drought tolerance. It was noted that the line ‘LP 11-1525/1’ achieved good results for most of the growth parameters studied, and also the lines derived from true potato seeds behaved well, in some cases even exceeding the line derived from meristems. Of the lines derived from true potato seeds, the best performance was noted for line ‘LI 101-6’ in all the analysed parameters, both on sorbitol and PEG medium. In addition, lines ‘LI 101-7’ and ‘LI 102-4’ achieved good results on both variants of medium used to mediate water stress. Therefore, establishing drought tolerance individuals within populations derived from true potato seeds using sorbitol and polyethylene glycol might be applied.

scholarly journals Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

2020 ◽  
pp. 57-64
Author(s):  
Arnaud Agbidinoukoun ◽  
Euloge Rimson Somakpe ◽  
Serge Sètondji Houedjissin ◽  
Florent Engelmann ◽  
Corneille Ahanhanzo
Keyword(s):  
Survival Rate ◽  
Cocos Nucifera ◽  
Coconut Palm ◽  
Zygotic Embryos ◽  
High Survival ◽  
Regeneration Rate ◽  
Surface Sterilization ◽  
Number Of Leaves ◽  
Cocos Nucifera L

Aims: This study aims to identify the best surface sterilization and evaluate the effect of haustorium suppression on in vitro germination of coconut palm (Cocos nucifera L.) zygotic embryos. Study Design: Survival rate and contamination rate of zygotic embryos after different surface sterilization treatments, regeneration rate and organogenesis through the number of leaves and the length of shoots after haustorium suppression were determined. For data processing, the Analysis of Variance was used to compare the means which were separated according to Tukey test            (P = 0.05). Place and Duration of Study: Coconut fruits (hybrid PB121) were collected 12 to 14 months after controlled pollination from CRAPP (Centre de Recherches Agricoles Plantes Pérennes), station of Sèmè-kpodji in Benin. Experiments were done in Central Laboratory of Plant Biotechnology and Plant Improvement, University of Abomey-Calavi and conducted from june to december in 2019. Methodology: For the zygotic embryos surface sterilization, four treatments combining three concentrations (3%, 6% and 15%) of commercial bleach (Javel la Croix© containing 12° active chlorine) and immersion durations (5 min, 10 min and 20 min) were tested and the survival rate were determined for each treatment after two months culture. The zygotic embryos were then divided in two sets (haustorium excised embryos set and the whole embryos set) and cultured in modified Y3 medium supplemented with 7 g L-1 agar, 2.5 g L-1 activated charcoal, 5% sucrose,  6.10-3 mM 2.4 D (2.4-dichlorophonoxyacetic acid), gibberellic acid and 0.3 mM BAP(6-benzylaminopurine). After five months culture, the regeneration rate, the number of leaves and the length of shouts were recorded. Results: The high survival rate (80%) was obtained with 6% of bleach and 20 min for the immersion duration without pre-disinfection. The suppression of haustorium have significantly increased the number of leaves (4.3 ± 0.02) and the length of shoots (16.2 ±0.7cm) compared to the whole zygotic embryos. Conclusion: This protocol can help to ensure better surface sterilization of zygotic embryos before their in vitro culture and the development of vigorous plantlets in order to improve the slow growth of plantlets, when transferred to the greenhouse or field. 

scholarly journals Short-term storage in vitro and large-scale propagation of grapevine genotypes

2012 ◽  
Vol 47 (3) ◽  
pp. 344-350 ◽  
Author(s):  
Rafael de Carvalho Silva ◽  
Zanderluce Gomes Luis ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Survival Rate ◽  
Large Scale ◽  
Culture Medium ◽  
Short Term ◽  
Minimal Growth ◽  
Short Term Storage ◽  
Term Storage ◽  
Storage Temperatures ◽  
Number Of Shoots

The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L-1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20ºC, can be micropropagated in large scale.

Cell wall proteins of in vitro cultured chili pepper lines differing in water stress tolerance

Plant Science ◽  
1997 ◽  
Vol 128 (2) ◽  
pp. 217-223 ◽  
Author(s):  
Quintero-Higuera Marı́a Fernanda ◽  
Santos-Dı́az Marı́a del Socorro ◽  
Garcı́a-de la Cruz Ramón Fernando
Keyword(s):  
Cell Wall ◽  
Water Stress ◽  
Stress Tolerance ◽  
Chili Pepper ◽  
Cell Wall Proteins ◽  
Water Stress Tolerance

Morphological and agronomical characteristics of coconut (Cocos nucifera L.) palms produced from in vitro cultured zygotic embryos

2013 ◽  
Vol 49 (5) ◽  
pp. 599-604 ◽  
Author(s):  
Yoboué Koffi ◽  
Oulo N’Nan-Alla ◽  
Jean-Louis Konan Konan ◽  
Bernard Malaurie ◽  
Florent Engelmann
Keyword(s):  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Cocos Nucifera L

scholarly journals EFFECT OF DIFFERENT BRANDS OF ACTIVATED CARBON ON GROWTH AND DEVELOPMENT OF COCONUT (Cocos Nucifera L) EMBRYOS IN VITRO

CORD ◽  
1998 ◽  
Vol 14 (02) ◽  
pp. 34
Author(s):  
Zosimo S. Bonaobra III ◽  
Erlinda P. Rillo ◽  
Osmundo D. Orense
Keyword(s):  
Growth And Development ◽  
Cocos Nucifera ◽  
Primary Root ◽  
Shoot Length ◽  
Zygotic Embryos ◽  
Percentage Germination ◽  
Significant Difference ◽  
Initial Culture ◽  
Cocos Nucifera L

The effect of different brands of activated charcoal [Merck GR (Art. 2186), Sigma acid washed (C‑4386), Sigma neutralized (C‑3790), and Duchefa neutralized (C‑1302)] on growth and development ofcoconut zygotic embryos in vitro was evaluated. Analysis of data noted after one month revealed that there was no significant difference on percentage germination, shoot length, and number ofprimary root of cv. Laguna Tall embryos cultured in Y3 liquid medium supplemented with 2.5gll AC of different brands. Length of primary root of embryos cultured in Duchefa neutralized AC was significantly different from those in Sigma neutralized AC only after one month from initial culture. For the succeeding periods (2‑4 months), no significant difiference was observed among the treatments in terms of increment in plant height, number of scale and true leaves and length ofprimary root. Statistical analysis revealed thatpercentage ofseedlings with primary, secondary and tertiary roots did not differ significantly among the treatments 1‑4 months from initial culture. Results suggest that any brand (even alternately) can be used satisfactorily in the in vitro culture of coconut embryos.

scholarly journals Evaluation of seedling growth of rice (Oryza sativa L.) genotypes under water stress and non-stress conditions

2020 ◽  
Vol 5 (2) ◽  
pp. 174-178
Author(s):  
Shreejana K. C. ◽  
Kabita Kumari Shah ◽  
Niru Baidhya ◽  
Prashamsa Neupane ◽  
Surakshya Pokhrel ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Drought Tolerance ◽  
Stress Condition ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Research Work ◽  
Higher Plant ◽  
Water Stress Condition ◽  
Number Of Leaves

Rice (Oryza sativa L.) is a species of crops which is highly sensitive to drought. The sensitivity to water stress varies from genotypes to genotypes of rice. An experiment was performed to compare twenty-five different rice genotypes for drought tolerance in the seedling stage at Gokuleshwor Agriculture and Animal Science College, Baitadi Nepal from July to August 2018. The genotypes were tested under two conditions viz., water stress and non-water stress. The research work was carried out in a completely random design (CRD) with three replications. The results exhibited the height of seedling, length & breadth of leaf, number of leaves/plant decreased, and number of leaves shedding increased under water stress condition. Clustering was done by the method of average linkage, and genotypes were categorized into five clusters. The genotype, namely Radha 11 grouped in Cluster-V had higher plant height, bigger length & breadth of leaf, maximum number of leaves/plant and minimum number of leaf shedding. Thus, this genotype showed the best performance for morphological traits under water stress condition. Therefore, this genotype Radha 11 can be utilized further for developing rice variety with drought-tolerance.

scholarly journals Bioprospecting rhizobacteria associated to cacti to water stress resistance and biofilm formation

2019 ◽  
Vol 6 (14) ◽  
pp. 873-881
Author(s):  
Clayton dos Santos Silva ◽  
João Manoel da Silva ◽  
José Ubaldo Lima de Oliveira ◽  
Romário Guimarães Verçosa de Araújo ◽  
Jessé Rafael Bento de Lima ◽  
...  
Keyword(s):  
Water Stress ◽  
Biofilm Formation ◽  
Culture Medium ◽  
Natural Occurrence ◽  
Tolerance Mechanisms ◽  
Isolation And Purification ◽  
Water Stress Tolerance ◽  
Naturally Occurring ◽  
Caatinga Vegetation ◽  
Promote Plant Growth

The aim of this study was to isolate and select bacteria from the cacti rhizosphere naturally occurring in an adverse environment in culture medium under water stress. Samples of rhizospheric cacti soil were collected in two distinct locations with natural occurrence of the Caatinga vegetation of the Alagoas State, Northeast Brazil. With the use of culture-dependent methodologies, the isolation and purification of the bacteria was performed for exopolysaccharide production analyzes by quantification of halos and biofilm formation in a spectrophotometer under optical density (OD) of 560 nm. In total, 42 strains were isolated, with the isolates BCM02, BCM06, BCM10 and BCM13 being the major producers of EPS, followed by bacteria BCM01, BCM05, BCM28 and BCM35 with medium production, and bacteria BCM22 and BCM33 with low production. On the other hand, for the biofilm formation, all the isolates were efficient in the synthesis of the same, being 38% of the bacteria with high formation performance. Thus, strains BCM02 and BCM28 presented the best results in evaluations of water stress tolerance mechanisms, and could later be used for future tests to promote plant growth.

Sign in / Sign up

Export Citation Format

Share Document