scholarly journals Pharmacognostic, Physicochemical and Anti-Oxidant Screening of Solenostemma Argel’s Leaf Extract

2020 ◽  
Vol 5 (9) ◽  
pp. 1202-1206
Author(s):  
Mehad Mustafa Adam Khamiss ◽  
Prakash C. Choudhary
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
New Approach ◽  
Anti Oxidant ◽  
Lead Generation ◽  
Prominent Response ◽  
Tribal Region ◽  
Physical And Chemical ◽  
Ethanolic Leaf Extract

The present work deals with the identification of new approach towards the lead generation in medicinal chemistry and authenticating the reports present on the use of this Sudanese medicinal plant Solenostemma argel in the tribal region of Sudan. The pharmacognostic screening showed its physical and chemical composition with identification of active ingredients. The antioxidant activity was carried out by DPPH and nitric oxide scavenging method. In which ethanolic leaf extract showed prominent response which may be due to the promising presence of flavonoids and related poly phenolic compounds in it

scholarly journals COMPARATIVE ANALYSIS OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL CONTENTS IN ETHANOLIC LEAF EXTRACTS OF IN VITRO AND FIELD GROWN WITHANIA SOMNIFERA

2016 ◽  
Vol 9 (5) ◽  
pp. 239 ◽  
Author(s):  
Prabhjot Singh Jassal ◽  
Gagandeep Kaur
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
Withania Somnifera ◽  
Leaf Extracts ◽  
Nitric Oxide Radical ◽  
In Vitro Plant ◽  
Ethanolic Leaf Extract ◽  
Phytochemical Contents

ABSTRACTObjective: The present study was planned to compare antioxidant activity in vitro and field grown Withania somnifera was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) assays. Medicinal plants are a major source of phytochemicals used for the treatments ofhuman diseases. W. somnifera has anti-inflammatory, antioxidant, and antimicrobial properties.Methods: Antioxidant activity and phytochemical contents in W. somnifera were determined spectrophotometrically.Results: The results of antioxidant activity of field grown ethanolic leaf extract of W. somnifera showed maximum inhibition of 72.08% and 77.85%in DPPH (50 µg/ml) and NO (100 µg/ml) scavenging assays, respectively. Field grown ethanolic leaf extract of W. somnifera showed maximumconcentrations of phenolics, flavonoids, and carotenoids, as active phytochemicals, determined spectrophotometrically, which were found as676.5 µg/ml, 557.5 µg/ml, and 469 µg/ml, respectively, as compared to in vitro plant extracts.Conclusions: This study demonstrated that antioxidant activity and phytochemical contents of field grown ethanolic leaf extract of W. somnifera werefound to be comparatively higher than in vitro plant extracts. Leaf extracts of W. somnifera are a potential source of antioxidants and could preventmany free radical-related diseases.Keywords: Carotenoids content, 1-diphenyl-2-picrylhydrazyl scavenging assay, Flavonoids content, Nitric oxide radical scavenging assay, Phenoliccontent.

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals Free Radical Scavenging and Antioxidant Potential of Ethanolic Leaf Extract of Diodia sarmentosa on High Fat Fed Wistar Rats

2020 ◽  
pp. 54-63
Author(s):  
Ezejiofor Tobias I. Ndubuisi ◽  
Korie Stephen Chinedumije ◽  
Peter Chukwudi
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Free Radical ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
High Fat ◽  
Ethanolic Leaf Extract

Diodia sarmentosa (Sw) commonly known as Zimbabwe flora or Tropical button weed is a straggling or procumbent perennial herb which grows in evergreen forest, riverine vegetation and bush land. It has been used traditionally for the treatment of ulcer, diabetes and other ailments. This study investigated the Free radical scavenging and antioxidant potential of ethanolic leaf extract of Diodia sarmentosa on high fat fed wistar rats. Thirty (30) male wistar rats (150 g-200 g) were divided into five (5) groups: Negative control rats, Positive control rats, Low dose extract rats treated with 250 mg/kg, High dose extract rats treated with 500mg/kg of the extract, and Standard antihyperlipidaemic drug rats treated with 5 mg/kg of Simvastatin. High fat diet was fed to the rats with Ghee and Coconut oil in the ratio of 3:1 for six (6) weeks, and administration of the treatments started from the 3rd week till the 6th week. The free radical scavenging and antioxidant potentials of Diodia sarmentosa was determined by assessing parameters like nitric oxide radical, hydroxyl radical, malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Glutathione S-transferase (GST) in the serum of the test rats. Diodia sarmentosa scavenged free radicals In vitro by inhibiting Nitric oxide and Hydroxyl radicals in a concentration-dependent manner, showing an inhibitory concentration at 50% (IC50) of 907.17 µg/ml ± 45.36 and 2173.44 µg/ml ± 100.11 respectively. Results obtained showed antioxidant enzymes like SOD, GPx and CAT in the serum of the test rats were significantly increased (p<0.05) by both doses of the extract when compared to the PC group and was similar to the standard drug used. GST activity was decreased in the serum by both doses of the extract, this decrease was not significant (p>0.05), while Malondialdehyde level was significantly decreased (p<0.05) by both doses of the extract in the serum of the tests rats. Diodia sarmentosa has antioxidants potentials because of its ability to scavenge free radicals, replenish antioxidant enzymes and reduce lipid peroxidation.

scholarly journals Antioxidant activity of alstonia Angustifolia ethanolic leaf extract

2017 ◽  
Author(s):  
Nurhidayah Ab Rahim ◽  
Noorzafiza Zakaria ◽  
Syarifah Masyitah Habib Dzulkarnain ◽  
Nazar Mohd Zabadi Mohd Azahar ◽  
Mahmood Ameen Abdulla
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanolic Leaf Extract

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND ESTIMATION OF TOTAL PHENOL AND FLAVONOID CONTENT OF ETHANOLIC LEAF EXTRACT OF IRIS KASHMIRIANA

2017 ◽  
Vol 10 (8) ◽  
pp. 309
Author(s):  
Shah Khalid ◽  
Aparna Alia ◽  
Shrivastava Pn ◽  
Muzafar Akbar Rather ◽  
Muzafar Ah Sheikh
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Reducing Power ◽  
Total Phenol ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Wide Range ◽  
Ethanolic Leaf Extract ◽  
In Vitro Antioxidant Activity

Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated.Methods: Total phenol content (TPC) was determined by Folin–Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay.Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25±0.57 μg/100 μg gallic acid equivalents and TFC of 33.61±3.37 μg/100 μg rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant.Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases.

scholarly journals Effect of Microencapsulation Techniques on Physical and Chemical Characteristics of Functional Beverage Based on Red Betel Leaf Extract (Piper crocatum)

2020 ◽  
Vol 23 (8) ◽  
pp. 276-282
Author(s):  
Mega Safithri ◽  
Susi Indariani ◽  
Rosalina Yuliani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Sensory Quality ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Functional Drink ◽  
Physical And Chemical

Functional drinks based on red betel leaf extract have antioxidant activity, but they still have a bitter taste. This study aims to determine the effect of microencapsulation on phenol content, antioxidant activity, and sensory quality of functional drinks based on betel leaf extract. Microencapsulation of functional drinks was made using maltodextrin coatings with concentrations of 10% and 20%. Antioxidant activity was tested by the CUPRAC method. The ready to drink (RTD) functional drink has a total phenolic content and antioxidant activity of 782.30 ± 2.54 mg GAE/g and 1660.19 ± 31.67 µmol Tr/g, respectively. These values are higher than microencapsulated functional drinks with maltodextrin (MM). The microencapsulated functional drink with 10% maltodextrin coating (MM10) is the chosen formulation since it has the smallest particle size (1.283 µm), total phenolic content of 12.90 ± 0.01 mg GAE/g and antioxidant activity of 189.41 ± 1.88 µmol Tr/g. Microencapsulated functional drinks provide sensory quality that is not significantly different (p <0.05) from ready to drink (RTD) drinks.

scholarly journals Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

2019 ◽  
Vol 18 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Nittaya Chansiw ◽  
Kamonnaree Chotinantakul ◽  
Somdet Srichairatanakool
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Stem Extract ◽  
Anti Inflammatory

Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. </P><P> Objective: To analyze active ingredients, total phenolic and total flavonoid contents, antiinflammatory and antioxidant activities from leaf and stem extracts of P. odoratum. </P><P> Methods: Leaves and stems were dried and extracted by using methanol, dichloromethane and water for obtaining Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MTE), Dichloromethane Leaf Extract (DLE), Dichloromethane Stem Extract (DTE), Water Leaf Extract (WLE) and Water Stem Extract (WTE). The extracts were quantified for total phenolic and total flavonoid contents by spectrophotometry and active compounds were analyzed by using GC-MS. Antioxidant activity was determined by ABTS and DPPH radicals scavenging assays. Anti-inflammatory activity was tested by the inhibition of nitric oxide production in RAW 264.7 macrophage cells induced by lipopolysaccharide. </P><P> Results: The DLE exhibited the most potent anti-inflammatory effect by inhibiting nitric oxide production in a concentration-dependent manner (IC50 = 53.75&#177;0.72 &#181;g/mL). MLE exhibited strong antioxidant activity and contained the highest concentration of phenolic compounds (52.59&#177;0.58 mg gallic acid equivalent/g extract) and flavonoid (19.97+0.11 mg quercetin equivalent/g extract). E-15-Heptadecenal and 3, 7, 11, 15-tetramethyl-2- hexadecen-1-ol were found predominantly in the methanol extracts. </P><P> Conclusion: The leaf extract of P. odoratum showed potent anti-inflammatory and antioxidant activities, mediated by DLE and MLE, respectively.

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