scholarly journals COMPARATIVE ANALYSIS OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL CONTENTS IN ETHANOLIC LEAF EXTRACTS OF IN VITRO AND FIELD GROWN WITHANIA SOMNIFERA

2016 ◽  
Vol 9 (5) ◽  
pp. 239 ◽  
Author(s):  
Prabhjot Singh Jassal ◽  
Gagandeep Kaur
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
Withania Somnifera ◽  
Leaf Extracts ◽  
Nitric Oxide Radical ◽  
In Vitro Plant ◽  
Ethanolic Leaf Extract ◽  
Phytochemical Contents

ABSTRACTObjective: The present study was planned to compare antioxidant activity in vitro and field grown Withania somnifera was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) assays. Medicinal plants are a major source of phytochemicals used for the treatments ofhuman diseases. W. somnifera has anti-inflammatory, antioxidant, and antimicrobial properties.Methods: Antioxidant activity and phytochemical contents in W. somnifera were determined spectrophotometrically.Results: The results of antioxidant activity of field grown ethanolic leaf extract of W. somnifera showed maximum inhibition of 72.08% and 77.85%in DPPH (50 µg/ml) and NO (100 µg/ml) scavenging assays, respectively. Field grown ethanolic leaf extract of W. somnifera showed maximumconcentrations of phenolics, flavonoids, and carotenoids, as active phytochemicals, determined spectrophotometrically, which were found as676.5 µg/ml, 557.5 µg/ml, and 469 µg/ml, respectively, as compared to in vitro plant extracts.Conclusions: This study demonstrated that antioxidant activity and phytochemical contents of field grown ethanolic leaf extract of W. somnifera werefound to be comparatively higher than in vitro plant extracts. Leaf extracts of W. somnifera are a potential source of antioxidants and could preventmany free radical-related diseases.Keywords: Carotenoids content, 1-diphenyl-2-picrylhydrazyl scavenging assay, Flavonoids content, Nitric oxide radical scavenging assay, Phenoliccontent.

scholarly journals In vitro pharmacological attributes and metabolite’s fingerprinting of Conocarpus lancifolius

2021 ◽  
Vol 20 (6) ◽  
pp. 660-671
Author(s):  
Syed Ali Raza ◽  
◽  
Maqsood Ahmad ◽  
Muhammad Waseem Mumtaz ◽  
Sadia Bashir ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanolic Extract ◽  
Acetylcholine Esterase ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Phenolic Contents ◽  
Freeze Dried ◽  
Ethanolic Extraction ◽  
Ethanolic Leaf Extract

Search for safe antioxidants and novel nutraceuticals urged to evaluate the antioxidant, anti-acetylcholine esterase and anti-lipoxygenase activity of various leaf extracts of Conocarpus lancifolius. Extraction was optimized from freeze dried plant extracts quenched with liquid nitrogen using water, ethanol, methanol, hexane, ethyl acetate and chloroform. Maximum extract yield, total phenolic contents and total flavonoid contents were obtained in case of ethanolic extraction. The highest 2,2-diphenyl-1-picrylhydrazylradical scavenging in terms of IC50 value of 55.26 µg/mL was observed for ethanolic leaf extract. The acetylcholine esterase and lipoxygenase inhibitory activities (IC50) were also observed for ethanolic extract. These findings for ethanolic extract were statistically significant when compared with other extracts (ρ<0.05). The haemolytic % values indicated that all extracts were associated with very low or negligible toxicity. The epicatechin, isorhamnetin, rutin, scopoleptin, skimmianine, quercetin-3-O-α-rhamnoside, quercetin-3-O-β-glucoside, cornoside, creatinine, choline, pyruvic acid, α-hydroxybutyric acid, phyllanthin and hypophyllanthin were identified as major functional metabolites in ethanolic leaf extract of C. lancifolius by 1H-NMR. The identified metabolites were probably responsible for the pharmacological properties of C. lancifolius. The findings may be utilized as pharmacological leads for drug development and food fortification.

scholarly journals In vitro Antimicrobial Activity of Ethanolic Leaf Extracts of Hibiscus Asper Hook. F. and Hibiscus Sabdariffa L. on some Pathogenic Bacteria

2021 ◽  
Vol 2 (3) ◽  
pp. 1-12
Author(s):  
Joseph Olowo Arogbodo ◽  
Oyetayo Bolanle Faluyi ◽  
Festus Omotere Igbe
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Hibiscus Sabdariffa ◽  
Bacterial Isolates ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity ◽  
Significant Difference ◽  
Ethanolic Leaf Extract

Purpose: The study aims to assess the antimicrobial activity of ethanolic leaf extracts of Hibiscus asper and Hibiscus sabdariffa against eight bacterial isolates. Materials and Methods:  An in vitro Antimicrobial activity of ethanolic leaf extract of the two plants against eight nosocomical and pathogenic bacteria viz; Pseudomonas aeruginosa (PAE), Proteus vulgaris (PVU), Klebsiella aerogenes (KAE), Staphylococcus aureus (SAU), Bacillus cereus (BCE), Escherichia coli (ECO), Moraxella catarrhalis (MCA) and Salmonella typhi (STY) was carried out using agar well diffusion assay with the concentration range of 3.13 – 100 mg/mL. Results: H. asper and H. sabdariffa showed significant difference (p< 0.05) in antimicrobial activity against BCE over the rest of the isolates. Inhibition zone diameters exhibited by the isolates to ethanolic leaf extract of H. asper was in descending order of BCE (15.00 ± 1.00a) >ECO (11.67 ± 0.58b) >SAU (7.67 ± 0.58c) >PAE (6.67 ± 0.58d) >STY (5.67 ± 0.58e) while that of H. sabdariffa was in the order BCE (15.33 ± 1.15a) > MCA (11.33 ± 1.15b) > SAU (11.00 ± 1.00bc) > KAE (9.67 ± 0.58c) > PAE (8.00 ± 1.00d) >PVU (7.67 ± 0.57e). PVU, KAE and MCA were resistant to the extract of H. asper while only STY was resistant to that of H. sabdariffa. Conclusion: H. sabdariffa extract demonstrated higher antimicrobial activity against the selected bacterial isolates than H. asper. However, the two extracts minimum inhibition concentrations (MICs) ranged from 25 mg/mL to 12.5 mg/mL. This is worthy of further exploration by pharmacological industries in the formulation of potent broad spectrum antibiotics for combating the present health challenge due to antimicrobial resistance.

scholarly journals Comparative Study of Leaf and Rootstock Aqueous Extracts of Foeniculum vulgare on Chemical Profile and In Vitro Antioxidant and Antihyperglycemic Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Karima Sayah ◽  
Nasreddine El Omari ◽  
Mourad Kharbach ◽  
Abdelhakim Bouyahya ◽  
Rabie Kamal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Leaf Extract ◽  
Quinic Acid ◽  
Leaf Extracts ◽  
Foeniculum Vulgare ◽  
Main Compound ◽  
Phenolic Components ◽  
Antidiabetic Effects

Foeniculum vulgare is a medicinal plant used in Moroccan folk medicine to treat several diseases such as diabetes. The aim of this study was to determine the phenolic bioactive compounds and to evaluate the antioxidant and antihyperglycemic activities of Foeniculum vulgare leaf and rootstock extracts. Phenolic compounds of F. vulgare rootstock and leaf extracts were determined using HPLC-DAD-QTOFMS analysis. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radicals. Moreover, the in vitro antihyperglycemic effects were tested by measuring the inhibition of α-amylase and α-glucosidase activities. HPLC-DAD-QTOFMS analysis identified thirty-two phenolic components in both leaf and rootstock extracts. Caffeic acid, quinic acid, and chlorogenic acid were the major compounds of F. vulgare leaf extract (FVLE), while the main compound of F. vulgare rootstock extracts (FVRE) was quinic acid. In the DPPH assay, F. vulgare leaf extract showed important antioxidant activity (IC50 = 12.16 ± 0.02 μg/mL) than F. vulgare rootstock extract (IC50 = 34.36 ± 0.09 μg/mL). Moreover, fennel leaf extracts revealed also the most powerful antioxidant activity (IC50 = 22.95 ± 0.4 μg/mL) in the ABTS assay. The in vitro antihyperglycemic activity showed that F. vulgare rootstock extract exhibited a remarkable inhibitory capacity (IC50 = 194.30 ± 4.8 μg/mL) of α-amylase compared with F. vulgare leaf extract (IC50 = 1026.50 ± 6.5 μg/mL). Furthermore, the inhibition of α-glucosidase was more importantly with F. vulgare rootstock (IC50 of 165.90 ± 1.2 μg/mL) than F. vulgare leaf extracts (203.80 ± 1.3 μg/mL). The funding of this study showed that F. vulgare rootstock and leaf extracts presented several phenolic compounds and showed important antioxidant and antidiabetic effects. We suggest that the identified molecules are responsible for the obtained activities. However, further studies focusing on the isolation and the determination of antioxidant and antidiabetic effects of F. vulgare rootstock and leaf main compounds are required.

scholarly journals Pharmacognostic, Physicochemical and Anti-Oxidant Screening of Solenostemma Argel’s Leaf Extract

2020 ◽  
Vol 5 (9) ◽  
pp. 1202-1206
Author(s):  
Mehad Mustafa Adam Khamiss ◽  
Prakash C. Choudhary
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
New Approach ◽  
Anti Oxidant ◽  
Lead Generation ◽  
Prominent Response ◽  
Tribal Region ◽  
Physical And Chemical ◽  
Ethanolic Leaf Extract

The present work deals with the identification of new approach towards the lead generation in medicinal chemistry and authenticating the reports present on the use of this Sudanese medicinal plant Solenostemma argel in the tribal region of Sudan. The pharmacognostic screening showed its physical and chemical composition with identification of active ingredients. The antioxidant activity was carried out by DPPH and nitric oxide scavenging method. In which ethanolic leaf extract showed prominent response which may be due to the promising presence of flavonoids and related poly phenolic compounds in it

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND ESTIMATION OF TOTAL PHENOL AND FLAVONOID CONTENT OF ETHANOLIC LEAF EXTRACT OF IRIS KASHMIRIANA

2017 ◽  
Vol 10 (8) ◽  
pp. 309
Author(s):  
Shah Khalid ◽  
Aparna Alia ◽  
Shrivastava Pn ◽  
Muzafar Akbar Rather ◽  
Muzafar Ah Sheikh
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Reducing Power ◽  
Total Phenol ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Wide Range ◽  
Ethanolic Leaf Extract ◽  
In Vitro Antioxidant Activity

Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated.Methods: Total phenol content (TPC) was determined by Folin–Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay.Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25±0.57 μg/100 μg gallic acid equivalents and TFC of 33.61±3.37 μg/100 μg rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant.Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases.

scholarly journals Evaluation of antioxidant property and anticancer prospective of the leaf extract and biofractions of Bauhinia foveolata Dalzell – A native of the Indian Western Ghats

2021 ◽  
Vol 12 (3) ◽  
pp. 1886-1894
Author(s):  
Liesl Maria Fernandes e Mendonça ◽  
Arun B. Joshi ◽  
Anant V. Bhandarkar ◽  
Himanshu Joshi
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Gallic Acid ◽  
Leaf Extract ◽  
Antioxidant Property ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Significant Activity

The current study was designed to evaluate the antioxidant and anticancer potential of ethanolic leaf extract of Bauhinia foveolata  Dalzell. (EEBF) and its toluene, ethyl acetate and methanolic biofractions viz., TFBF, EFBF and MFBF. Phytoconstituents were screened by adopting established procedures. Total phenolic and flavonoid content were assessed spectrophotometrically. In vitro antioxidant activity was assayed using DPPH (2,2-diphenyl-1-picrylhydrazyl), hydrogen peroxide and nitric oxide as free radicals, whereas anticancer activity was evaluated using sulforhodamine B assay.  EEBF showed maximum phenolic content of 49.12±0.31 mg GAE/g and flavonoidal content of 28.75±0.42 mg QUE/g, than its biofractions. EEBF showed considerable antioxidant activity with IC50=19.04±0.24 μg/mL and IC50=65.85±1.22 μg/mL when compared to the standards Ascorbic acid (IC50=12.06±0.05 μg/mL) and Gallic acid (IC50=64.65±0.72  μg/mL) in DPPH and nitric oxide scavenging assays, respectively. MFBF showed significant activity with IC50=26.76±0.75 μg/mL in hydrogen peroxide scavenging assay compared to the standard Gallic acid (IC50=76.60±1.31 μg/mL).  TFBF showed favourable growth inhibition of MCF-7 cells  with GI50=73.5±11.96 µg/mL when compared to other samples screened (GI50>80 μg/mL) as against the standard Adriamycin (GI50<10 μg/mL) in SRB assay. The therapeutic virtues of EEBF and MFBF as free radical scavengers and TFBF as an antiproliferative may be attributed to the phenolics, flavonoids, steroids and triterpenoids present.

scholarly journals Phytochemical Investigation and Antioxidant Activity of Leaf Extract of Withania somnifera from Konso, South Ethiopia

2018 ◽  
Vol 34 (4) ◽  
pp. 1824-1831 ◽  
Author(s):  
Tesfaye Tebeka Simur
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Leaf Extract ◽  
Withania Somnifera ◽  
Reducing Power ◽  
Dry Weight ◽  
Leaf Extracts ◽  
South Ethiopia ◽  
Total Antioxidant

Withania somnifera is endemic to Ethiopia where its leaf and root is traditionally used for the treatment of malaria in south Ethiopia. The main purpose of this study was to investigate phytochemicals and antioxidant activity of the leaf extract of Withania somnifera. The anti-oxidant potential and total antioxidant potential was determined by using reducing power determination method and phosphomolb date method respectively. As per the phytochemical tests, the methanol extract revealed that presence of tannins, phenolics, flavonoids, saponins and, alkaloids while all are absent in hexane extract. The antioxidant capacity of methanol, acetone and hexane leaf extracts of Withania somnifera were found to be 494.088±0.230, 104.190±0.020 and 3.413±0.023 mg of ascorbic acid per 10 mg of dry weight of antioxidant in the reducing power, respectively and it’s total antioxidant capacity of these extracts were 45.41±0.018, 8.70±0.004 and 0.38±0.002mg of ascorbic acid per 10 mg of dry weight of the crude extract. Thus, the results of the present investigation infer that this plant extracts possess potent antioxidant. Therefore it is strongly recommended to isolate the antioxidant components.

Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

2020 ◽  
Vol 16 ◽  
Author(s):  
Maibam Beebina Chanu ◽  
Biseshwori Thongam ◽  
Khumukcham Nongalleima ◽  
Hans Raj Bhat ◽  
Surajit Kumar Ghosh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Quercus Serrata ◽  
Control Group ◽  
Blood Cell Count ◽  
Toxicological Profile

Background: Quercus serrata Murray leaves have been used traditionally in the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study had been reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study was aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28-days. Control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum, the blood-cell count was done using whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, DPPH free-radical scavenging assay were performed to check the antioxidant activity of the extract. Results: There were no significant alterations in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with IC50 4.48±0.254 µg/mL, in-vitro reducing power activity with IC50121.65±0.320 µg/mL and nitric oxide scavenging activity IC50 106.43±0.338 µg/mL. Conclusion: The above study showed that standardized methanolic extract of Quercus serrata leaves was safe after subacute oral administration in rats and has good antioxidant potential.

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