Antioxidant activity of alstonia Angustifolia ethanolic leaf extract

ABSTRACTObjective: The present study was planned to compare antioxidant activity in vitro and field grown Withania somnifera was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) assays. Medicinal plants are a major source of phytochemicals used for the treatments ofhuman diseases. W. somnifera has anti-inflammatory, antioxidant, and antimicrobial properties.Methods: Antioxidant activity and phytochemical contents in W. somnifera were determined spectrophotometrically.Results: The results of antioxidant activity of field grown ethanolic leaf extract of W. somnifera showed maximum inhibition of 72.08% and 77.85%in DPPH (50 µg/ml) and NO (100 µg/ml) scavenging assays, respectively. Field grown ethanolic leaf extract of W. somnifera showed maximumconcentrations of phenolics, flavonoids, and carotenoids, as active phytochemicals, determined spectrophotometrically, which were found as676.5 µg/ml, 557.5 µg/ml, and 469 µg/ml, respectively, as compared to in vitro plant extracts.Conclusions: This study demonstrated that antioxidant activity and phytochemical contents of field grown ethanolic leaf extract of W. somnifera werefound to be comparatively higher than in vitro plant extracts. Leaf extracts of W. somnifera are a potential source of antioxidants and could preventmany free radical-related diseases.Keywords: Carotenoids content, 1-diphenyl-2-picrylhydrazyl scavenging assay, Flavonoids content, Nitric oxide radical scavenging assay, Phenoliccontent.

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Pharmacognostic, Physicochemical and Anti-Oxidant Screening of Solenostemma Argel’s Leaf Extract

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20sep780 ◽

2020 ◽

Vol 5 (9) ◽

pp. 1202-1206

Author(s):

Mehad Mustafa Adam Khamiss ◽

Prakash C. Choudhary

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Leaf Extract ◽

New Approach ◽

Anti Oxidant ◽

Lead Generation ◽

Prominent Response ◽

Tribal Region ◽

Physical And Chemical ◽

Ethanolic Leaf Extract

The present work deals with the identification of new approach towards the lead generation in medicinal chemistry and authenticating the reports present on the use of this Sudanese medicinal plant Solenostemma argel in the tribal region of Sudan. The pharmacognostic screening showed its physical and chemical composition with identification of active ingredients. The antioxidant activity was carried out by DPPH and nitric oxide scavenging method. In which ethanolic leaf extract showed prominent response which may be due to the promising presence of flavonoids and related poly phenolic compounds in it

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EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND ESTIMATION OF TOTAL PHENOL AND FLAVONOID CONTENT OF ETHANOLIC LEAF EXTRACT OF IRIS KASHMIRIANA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i8.18268 ◽

2017 ◽

Vol 10 (8) ◽

pp. 309

Author(s):

Shah Khalid ◽

Aparna Alia ◽

Shrivastava Pn ◽

Muzafar Akbar Rather ◽

Muzafar Ah Sheikh

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Reducing Power ◽

Total Phenol ◽

Flavonoid Content ◽

Dpph Assay ◽

Wide Range ◽

Ethanolic Leaf Extract ◽

In Vitro Antioxidant Activity

Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated.Methods: Total phenol content (TPC) was determined by Folin–Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay.Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25±0.57 μg/100 μg gallic acid equivalents and TFC of 33.61±3.37 μg/100 μg rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant.Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases.

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Phytochemical composition, in vitro antioxidant activity and acute toxicity of Irvingia gabonensis (O’Rorke) baill ethanolic leaf extract

International Journal of Biological Research ◽

10.14419/ijbr.v4i1.5939 ◽

2016 ◽

Vol 4 (1) ◽

pp. 36 ◽

Cited By ~ 2

Author(s):

Efosa Ewere ◽

Emmanuel Uka ◽

Usunomena Usunobun

Keyword(s):

Antioxidant Activity ◽

Body Weight ◽

Acute Toxicity ◽

Leaf Extract ◽

Phenolic Content ◽

Reducing Power ◽

Total Phenolic ◽

Phytochemical Composition ◽

Irvingia Gabonensis ◽

Ethanolic Leaf Extract

<p>This study was aimed at determining the phytochemical composition, antioxidant effect and acute toxicity of Irvingia gabonensis (O’Rorke) baill (IG) ethanolic leaf extract. Qualitative phytochemcal analysis was carried out on the ethanolic leaf extract using standard procedures. Different concentrations of the plant extract (20 µg/ml- 100µg/ml) were used to assess its effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical activity, It's reducing power and it total phenolic content. Lorke’s method of acute toxicity was adopted for the acute toxicity study. Results obtained showed the presence of saponins, tannins, flavonoids, cardiac glycosides, steroids, and carbohydrates in the leaf extract. The ethanolic leaf extract of IG significantly (P< 0.05) inhibited the activity of DPPH when compared with the vitamin C standard. IG leaf extract also showed a higher reducing power as compared with the Vitamin C standard. The total phenolic content of IG ethanolic leaf extract was however, significantly (P<0.05) lower than that of gallic acid, the standard used. Futhermore, the LD50 of the ethanolic leaf extract was found to be above 5000mg/kg body weight. Irvingia gabonensis (O’Rorke) baill ethanolic leaf extract is a rich source of important phytochemicals and possesses a high antioxidant activity. Also, the administration of the ethanolic leaf extract in wistar rats is safe up to a dose of 5000mg/kg body weight.</p>

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