scholarly journals Effects of pelvic floor bioelectrical stimulation on frozen embryo transfer patients with thin endometrium

2019 ◽  
Vol 17 ◽  
pp. 205873921882286
Author(s):  
Bing Yuan ◽  
Hong Zheng ◽  
Minling Xu ◽  
Xiaoli Mu ◽  
Fengcai Huang
Keyword(s):  
Pelvic Floor ◽  
Embryo Transfer ◽  
Embryo Implantation ◽  
Implantation Rate ◽  
Frozen Embryo Transfer ◽  
Control Group ◽  
Observation Group ◽  
Thin Endometrium ◽  
Significant Difference ◽  
Artificial Cycle

This study was to observe the effects of pelvic floor bioelectrical stimulation on pregnancy outcome and serum estradiol (E2) and progesterone (P) levels in frozen embryo transfer patients with thin endometrium. 120 cases frozen embryo transfer patients with thin endometrium in our hospital from March 2016 to April 2017 were selected. These patients received artificial cycle replacement plan before embryo transfer. According to whether pelvic floor bioelectrical stimulation therapy was accepted, 120 cases of thin endometrial frozen embryo transfer were divided into control group and observation group. 50 cases of the control group received artificial cycle to prepare for embryo transplantation, while 70 cases of observation group received bioelectrical stimulation as intervention treatment on the basis of artificial cycle. The intima thickness, subintimal hemodynamic parameters, embryo implantation rate, and clinical pregnancy rate were compared between the two groups before and after treatment. There was no significant difference in endometrial thickness between the two groups on the 10th day of menstruation ( P > 0.05). The thickness of endometrium and the index of intima growth in the observation group were significantly higher than those in the control group ( P < 0.05). The pulsatility index, resistance index, and systolic and diastolic blood flow velocity ratio of subintimal blood flow in the observation group were significantly higher than those in the control group ( P < 0.05). Before treatment, there was no significant difference in serum E2 and P between the two groups ( P > 0.05). After treatment, the improvement of serum E2 and P in the observation group was significantly better than that of the control group ( P < 0.05). The embryo implantation rate (25.7%) in the observation group was significantly higher than that in the control group (17.0%) ( P < 0.05). The clinical pregnancy rate (47.1%) in the observation group was significantly higher than that in the control group (30%) ( P < 0.05). In conclusion, the intervention of pelvic floor bioelectrical stimulation can improve the blood perfusion of thin endometrium, improve the endometrial receptivity, increase the implantation rate of frozen embryo transfer in patients with thin endometrium, and improve the level of serum estradiol and progesterone.

scholarly journals The effect of catheter rotation during its withdrawal on frozen thawed embryo-transfer cycles outcomes: A Case-control study

2019 ◽  
Author(s):  
Maryam Eftekhar ◽  
Lida Saeed ◽  
Masrooreh Hoseini
Keyword(s):  
Embryo Transfer ◽  
Case Control Study ◽  
Implantation Rate ◽  
Case Control ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Control Group ◽  
Significant Difference ◽  
Control Study ◽  
Frozen Thawed Embryo Transfer

Background: Embryo transfer (ET) is the last and the most clinical process in assisted reproductive technology cycle. It has been suggested that cervical mucus interacts with an adequate embryo transfer in different ways. A few studies showed that catheter rotation could discharge mucus entrapped in the embryo to neutralize embryo displacement. Objective: The aim of this present study was to compare the outcome of frozen embryo transfer (FET) based on catheter rotation during withdrawal. Materials and Methods: In this case-control study, the clinical documents of 240 women who experienced frozen embryo transfer cycles were reviewed. The subjects were divided into two groups (n = 120/each), including A) the rotation treatment group (360°) that underwent ET using catheter rotation and B) the control group including the subjects who experienced ET with no catheter rotation. Clinical and chemical pregnancies and implantation rates were compared between two groups. Results: Results showed that there is no significant difference between the basic clinical and demographic features of both groups (p > 0.05). A significant difference was observed in terms of the rate of chemical pregnancy between groups (21.7% vs 43.3%, p = 0.001 respectively). In addition, the rate of clinical pregnancy was significantly higher in study group than the control (33.35% vs 14.2%, p = 0.002, respectively). Conclusion: Our results demonstrated that catheter rotation during withdrawal increased the implantation rate and clinical pregnancy.

scholarly journals Granulocyte Colony-Stimulating Factor Combined With Transcutaneous Electrical Acupoint Stimulation in Treatment of Unresponsive Thin Endometrium in Frozen Embryo Transfer Cycles

2021 ◽  
Vol 3 ◽  
Author(s):  
Linjiang Song ◽  
Qinxiu Zhang ◽  
Shaomi Zhu ◽  
Xudong Shan
Keyword(s):  
Embryo Transfer ◽  
Endometrial Thickness ◽  
Embryo Implantation ◽  
Implantation Rate ◽  
Granulocyte Colony Stimulating Factor ◽  
Thin Endometrium ◽  
Treatment Groups ◽  
Stimulating Factor ◽  
Embryo Implantation Rate ◽  
Acupoint Stimulation

Objective: This trial was designed to assess the treatment effects of granulocyte colony-stimulating factor (G-CSF) and transcutaneous electrical acupoint stimulation (TEAS) on thin endometrium in frozen-thawed embryo transfer (FET) cycles.Methods: Ninety-nine patients with previous cancellations of embryo transfer were included, 56 of whom were prospectively treated with intrauterine perfusion of G-CSF in subsequent FET cycles. The selected patients were randomized into the G-CSF perfusion only group and the G-CSF perfusion combined with TEAS group. The other 43 patients were retrospectively included as controls.Results: Compared to previous cycles, endometrial thickness was statistically significantly increased in the two treatment groups (5.97 ± 0.60, 7.52 ± 0.56, 6.14 ± 0.52, and 7.66 ± 0.44; P = 0.00 and 0.00, respectively). The increases in endometrial thickness suggested that no statistically significant difference was found between the two treatment groups. The G-CSF with TEAS group suggested a higher embryo implantation rate than the G-CSF perfusion only and control groups (33.33 and 29.1% and 33.33 and 17.39%; P = 0.412 and 0.091, respectively). The G-CSF combined with TEAS group demonstrated nominally higher clinical and ongoing pregnancy rates than the G-CSF perfusion-only group and controls, though, the difference was not statistically significant.Conclusion: G-CSF has a potential role in improving endometrium thickness in patients with thin unresponsive endometrium in FET treatment cycles. In addition, when combined with TEAS, G-CSF perfusion treatment also improves the embryo implantation rate; however, randomized controlled trials are highly demanded to provide high-grade evidence regarding clinical pregnancy rate after G-CSF perfusion treatment.

scholarly journals Effects of Laser Assisted Hatching on Pregnancy Outcomes of Frozen Embryo Transfer Cycles

2020 ◽  
Vol 5 (9) ◽  
pp. 1223-1223
Author(s):  
Dr. Narmadha. R ◽  
Dr . Manjula ◽  
Dr. N.Sanjeeva Reddy ◽  
Dr. Sindhuja. N.S
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Zona Pellucida ◽  
Implantation Rate ◽  
Frozen Embryo Transfer ◽  
Control Group ◽  
Assisted Hatching ◽  
Younger Women ◽  
The Mean ◽  
Baseline Characteristics

The implantation of the embryo into the uterus requires hatching from its zona pellucida (ZP). The inability of the embryo to break its zona pellucida is considered as a factor for implantation failure. Assisted hatching (AH) is performed to make it easier for natural hatching to occur, also providing early embryoendometrium contact, which favors the embryos implantation into the uterus. To evaluate the effect of laser assisted hatching (LAH) on pregnancy rate in frozen embryo transfer cycle.  Materials & methods: In a prospective observational study a total of 80 patients who underwent frozen embryo transfer(FET) cycles were included in the study. Patients were divided into Laser assisted hatching group (LAH) and no LAH group. In the LAH group, zona thinning was done with the help of laser just prior to the embryo transfer. In the control group no hatching was done before transfer. The main outcome measures were pregnancy rate & implantation rate. The baseline characteristics of the 80 patients included in the study ie.,LAH group (n=40) vs no LAH group (n=40),the mean age ( 30.855.4 vs 333.9),mean BMI (25.64.1 vs 27.94.5),duration of infertility ( 6.184.1 vs 8.534.7) , number of embryos transferred (2.750.8 vs 2.70.72) respectively. Younger women < 30 years in no LAH group had higher pregnancy rate compared to the LAH group. Between the two groups, patients between 31-35 years in LAH group had higher pregnancy rate compared to no LAH group. The pregnancy rate (42.85% vs 54.5%) in LAH group of women >35years found lower than the no LAH group. LAH seems to be beneficial in women between 31- 35 years of age group, but LAH does not seem to be beneficial in women > 35 years of age. There is slight increase in pregnancy rates with laser assisted zona thinning in frozen transfer cycle, but it is not statistically significant.

The Influence of the Pelvic Floor Muscle Massage on Pregnancy Outcome in Patients Treated with Frozen-Thawed Embryo Transfer

2021 ◽  
Vol 7 (5) ◽  
pp. 1842-1852
Author(s):  
Longying Shen ◽  
Chang Liu
Keyword(s):  
Pelvic Floor ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Endometrial Thickness ◽  
Pelvic Floor Muscle ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Observation Group ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Objective: To research the influence of the pelvic floor muscle massage on pregnancy outcome of patients treated with frozen-thawed embryo transfer (FET). Methods: We choose 120 patients who will have the frozen-thawed embryo transfer after it is unfreezed, and they are departed into two groups averagely, 60 of observation group and 60 of control group. Both groups start taking estradiol valerate twice a day on the third day of the menstrual cycle, 3mg one time. 14 days later, all patients need to inject progesterone except taking estradiol valerate. The observation group: Starting the pelvic floor muscle massage from the end of menstruation till the day of injecting progesterone, 15-20 minutes, 50 HZ, the acupoints are huiyin and baliao. The contrast group doesn't have any other therapeutic measures. We will transfer the high-quality embryo on the suitable day. We contrast the basic conditions, the embryo, the endometrial thickness and types of uterine endometrium. To analyze the endometrial blood type, PI, RI, S/D, biochemical pregnancy rate, clinical pregnancy rate and early abortion rate. Results: There are no statistical result in transferring conditions, the endometrial thickness and types of uterine endometrium. After the treatment, we find that: 1. in the observation group, after the treatment of pelvic floor muscle massage ,the endometrial blood of the menstrual 17th day compared with the menstrual 9th day, type I decreases clearly, type If and B increase evidently(p<0.05);2. in the observation group after the treatment of pelvic floor muscle massage, we get that the indexes RI, PI and S/D of the menstrual 17th day compared with the menstrual 9th day , reduced remarkably(p<0.05);3 the observation group of patients after treatment, the menstrual 17th day endometrial blood flow decreased significantly than control group in blood flow I type, type If + B increased significantly,(p < 0.05) ;4. The observation group of patients after treatment, PI, RI and S/D of the menstrual 17th days was lower than control group significantly (P < 0.0S);S. in the observation group of patients after treatment ,the endometrial thickness compared with the control group was no statistically different (p>0.05), but the endometrial thickness of the observation group was higher than the control group;6.the implantation rate and clinical pregnancy rate are higher in the observation group than those in the control group. Conclusion: The pelvic floor muscle massage improves the endometrial thickness and endometrial blood conditions, so as to increase the clinical pregnancy rate of patients with frozen-thawed embryo transfer (FET).

scholarly journals Effect of a single dose of ibuprofen lysinate before embryo transfer on pregnancy rates in cows

Reproduction ◽  
2001 ◽  
pp. 151-154 ◽  
Author(s):  
M Elli ◽  
B Gaffuri ◽  
A Frigerio ◽  
M Zanardelli ◽  
D Covini ◽  
...  
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Embryo Transfer ◽  
Embryo Implantation ◽  
Pregnancy Rates ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Significant Difference ◽  
Enzyme Isoforms ◽  
And Control

Embryo implantation is a critical step in both cows and humans. The use of ibuprofen lysinate to enhance implantation has been investigated in cattle with the specific aim of improving pregnancy rates after embryo transfer. In this study, heifers (n = 100) were assigned randomly to one of two groups: one group was treated i.m. with 5 mg ibuprofen lysinate kg(-1) body weight 1 h before embryo transfer and a control group received vehicle only. A single embryo was transferred into each recipient cow. There was a significant difference in the number of pregnancies after embryo transfer between cows in the treated (41 of 50; 82%) and control (28 of 50; 56%) groups (P < 0.05). These data indicate that ibuprofen lysinate may be an effective adjunctive treatment for assisted reproduction in cattle. Further studies are needed to clarify whether this effect is associated with the reduction of cyclooxygenase enzyme isoforms during embryo transfer or whether other mechanisms are involved.

scholarly journals Evaluating of the Pregnancy Rate of Freezing Embryo Transfer in the Presence or Absence of GnRH Agonist

2021 ◽  
Vol 23 (6) ◽  
pp. 818-827
Author(s):  
Leila Naserpoor ◽  
◽  
Katayoun Berjis ◽  
Rahil Jannatifar ◽  
◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Gnrh Agonist ◽  
Assisted Reproductive Technologies ◽  
Implantation Rate ◽  
Study Groups ◽  
Reproductive Technologies ◽  
Abortion Rate ◽  
Frozen Embryo Transfer ◽  
Significant Difference

Background and Aim: Applying Assisted Reproductive Technologies (ARTs) is increasing. A critical step in ART is the frozen embryo transfer, in which the endometrium thickness has great significance in the outcome. In this case, the frozen embryo will be transferred during the next cycle. There are several ways to prepare an endometrium for transmitting embryos; however, choosing the best method remains debated. The present study aimed to evaluate the pregnancy rate of frozen embryo transfer in the presence or absence of GnRH agonists. Methods & Materials: A retrospective analysis was conducted on 146 consecutive patients attending Qom’s infertility treatment center from 2015 to 2017; these subjects were candidates for the transfer cycle of the frozen-thawed embryo and randomly assigned to receive either protocol with or without GnRH agonist. Clinical features, implantation rate, pregnancy rate (chemical & clinical), and abortion rate were assessed. Ethical Considerations: This study was approved by the Research Ethics Committee of the Academic Center for Education, Culture, and Research of Mashhad University (Code: IR.ACECR.JDM.REC.1398.001). Results: There was no significant difference in baseline and clinical characteristics, implantation rate, pregnancy rate (chemical & clinical), and abortion rate between the study groups of endometrial preparations with or without GnRH agonist (P<0.05). Conclusion: In this study, pregnancy outcome was similar in both study groups; thus, this method is recommended as an endometrial preparation without GnRH agonist.

scholarly journals Should rescue ICSI be re-evaluated considering the deferred transfer of cryopreserved embryos in in-vitro fertilization cycles? A systematic review and meta-analysis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Alessio Paffoni ◽  
Marco Reschini ◽  
Valerio Pisaturo ◽  
Cristina Guarneri ◽  
Simone Palini ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Implantation Rate ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Rescue Icsi

Abstract Background Total fertilization failure represents a particularly frustrating condition for couples undergoing in vitro fertilization. With the aim of reducing the occurrence of total fertilization failure, intracytoplasmic sperm injection (ICSI) has become the first choice over conventional in vitro fertilization (IVF) procedures although evidence of improved results is still debated and its use in couples without male factor infertility is not recommended. Among the strategies potentially useful to promote the use of conventional IVF, we herein call attention to the late rescue ICSI, which consists in performing ICSI after 18–24 h from conventional insemination on oocytes that show no signs of fertilization. This treatment has however been reported to be associated with a low success rate until recent observations that embryos derived from late rescue ICSI may be transferred after cryopreservation in a frozen-thawed cycle with improved results. The aim of the present study was to assess whether frozen embryos deriving from rescue ICSI performed about 24 h after conventional IVF may represent a valuable option for couples experiencing fertilization failure. Methods A systematic review on the efficacy of late rescue ICSI was performed consulting PUBMED and EMBASE. Results Including twenty-two original studies, we showed that clinical pregnancy rate per embryo transfer and implantation rate obtainable with fresh embryo transfers after rescue ICSI are not satisfactory being equal to 10 and 5%, respectively. The transfer of cryopreserved rescue ICSI embryos seems to offer a substantial improvement of success rates, with pregnancy rate per embryo transfer and implantation rate equal to 36 and 18%, respectively. Coupling rescue ICSI with frozen embryo transfer may ameliorate the clinical pregnancy rate for embryo transfer with an Odds Ratio = 4.7 (95% CI:2.6–8.6). Conclusion Results of the present review support the idea that r-ICSI coupled with frozen embryo transfer may overcome most of the technical and biological issues associated with fresh transfer after late r-ICSI, thus possibly representing an efficient procedure for couples experiencing fertilization failure following conventional IVF cycles. Trial registration Prospero registration ID: CRD42021239026.

scholarly journals Luteogenesis and Embryo Implantation Are Enhanced by Exogenous hCG in Goats Subjected to an Out-of-Season Fixed-Time Artificial Insemination Protocol

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 429
Author(s):  
Jorge A. Bustamante-Andrade ◽  
César A. Meza-Herrera ◽  
Rafael Rodríguez-Martínez ◽  
Zurisaday Santos-Jimenez ◽  
Oscar Ángel-García ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
Embryo Implantation ◽  
Implantation Rate ◽  
Fixed Time ◽  
Efficiency Index ◽  
Control Group ◽  
Condition Score ◽  
Interesting Approach

The aim of this study was to evaluate the possible effect of two doses of hCG (100 and 300 IU) applied at two different times (7 and 14 d) after a fixed-time artificial insemination protocol (FTAI) upon some variables involved in the embryonic implantation rate in goats during the natural deep anestrous season (April, 25° north). The experimental units considered crossbred, multiparous, anovulatory goats (n = 69, Alpine, Saanen, Nubian x Criollo), with average body weight (43.6 ± 5.7 kg) and body condition score (1.86 ± 0.28 units) located in northern–semiarid Mexico (25° N, 103° W). Once the goat’s anestrus status was confirmed, goats were subjected to an estrus induction protocol. Upon estrus induction confirmation, goats (n = 61) were subjected to a FTAI procedure. Immediately after the FTAI, the goats were randomly distributed to five experimental groups: (1). G100-7 (n = 13) 100 IU, hCG 7 d post-FTAI, (2). G100-14 (n = 12) 100 IU hCG, 14 d post-FTAI, (3). G300-7 (n = 12) 300 IU, hCG, 7 d post-FTAI, (4). G300-14 (n = 12) 300 IU hCG 14 d post-FTAI, and (5). Control group, CONT (n = 12) 0.5 mL saline, 7 and 14 d post-FTAI. The response variables conception rate (39.36 ± 0.23), fertility rate (27.96%), prolificacy rate (1.1 ± 0.29 kids), ovulation rate (0.74 ± 0.20 corpus luteum) corpus luteum diameter (10.15 ± 0.59 mm), embryo number (1.58 ± 0.20), and embryo implantation rate (48.96%), did not differ between treatments. However, while the variables fecundity rate (67%), embryo efficiency index-1 (33.99 ± 0.20%), and embryo efficiency index-2 (27.94 ± 0.30%) were favored by the G300-14 treatment, the corpus luteum area was favored (p < 0.05) by both G300-7 (113.30 ± 0.19 mm2) and G300-14 (103.04 ± 0.17 mm2). Such reproductive strategy emerges as an interesting approach, not only to enhance the out-of-season reproductive outcomes, but also to boost one of the main rulers defining the global reproductive efficiency of a heard, namely, the embryo implantation efficiency.

scholarly journals Natural Cycle Results in Lower Implantation Failure than Ovarian Stimulation in Advanced-Age Poor Responders Undergoing IVF: Fertility Outcomes from 585 Patients

2021 ◽  
Author(s):  
Maria Paola De Marco ◽  
Giulia Montanari ◽  
Ilary Ruscito ◽  
Annalise Giallonardo ◽  
Filippo Maria Ubaldi ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Ovarian Stimulation ◽  
Embryo Implantation ◽  
Implantation Rate ◽  
Poor Responder ◽  
Advanced Age ◽  
Poor Responders ◽  
Natural Cycle ◽  
Significant Difference ◽  
Over 40 Years

AbstractTo compare pregnancy rate and implantation rate in poor responder women, aged over 40 years, who underwent natural cycle versus conventional ovarian stimulation. This is a retrospective single-center cohort study conducted at the GENERA IVF program, Rome, Italy, between September 2012 and December 2018, including only poor responder patients, according to Bologna criteria, of advanced age, who underwent IVF treatment through Natural Cycle or conventional ovarian stimulation. Between September 2012 and December 2018, 585 patients were included within the study. Two hundred thirty patients underwent natural cycle and 355 underwent conventional ovarian stimulation. In natural cycle group, both pregnancy rate per cycle (6.25 vs 12.89%, respectively, p = 0.0001) and pregnancy rate per patient101 with at least one embryo-transfer (18.85 vs 28.11% respectively, p = 0.025) resulted significant reduced. Pregnancy rate per patient managed with conventional ovarian stimulation resulted not significantly different compared with natural cycle (19.72 vs 15.65% respectively, p = 0.228), but embryo implantation rate was significantly higher in patients who underwent natural cycle rather than patient subjected to conventional ovarian stimulation (13 vs 8.28% respectively, p = 0.0468). No significant difference could be detected among the two groups in terms of abortion rate (p = 0.2915) or live birth pregnancy (p = 0.2281). Natural cycle seems to be a valid treatment in patients over 40 years and with a low ovarian reserve, as an alternative to conventional ovarian stimulation.

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