Comparative antioxidant potential and total flavonoids in different extracts of pericarp and pulp of Garcinia cowa Roxb.

2011 ◽  
Vol 1 (4) ◽  
pp. 178-181
Author(s):  
A. K. Chaurasia ◽  
B. J. Gogoi ◽  
P. Chattopadhyay ◽  
H. K. Gogoi ◽  
Lokendra Singh
Keyword(s):  
Antioxidant Activity ◽  
Beta Carotene ◽  
Total Flavonoid ◽  
Fresh Mass ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Garcinia Cowa

The antioxidant and total flavonoid content (TFC) of solvent extracts of pericarpand pulp of Garcinia cowa Roxb. were evaluated. The antioxidant activitywas evaluated using 2, 2 Ã¢â‚¬Âdi phenyl‐1‐picrylhydrazyl (DPPH) free radicalscavenging assay and beta carotene linoleate bleaching ( βCL) assay. Ascorbicacid and butylated hydroxyl toluene (BHT) were used as the standards. Theethyl acetate extract of the pulp showed the highest inhibition (89.5 ±0.81%) of DPPH radical, along with the highest antioxidant activity (75.1 ±0.60%) in βCL assay and the highest total flavonoid content 67.4 ± 1.41 mgcatechin equivalent (CE)/100g fresh mass at 1000ppm concentration.

scholarly journals Antioxidant Effects of Ponciri Fructus Bioconversion Extracts as Cosmetic Ingredients

2021 ◽  
Vol 19 (3) ◽  
pp. 477-485
Author(s):  
Gyu Bin Kong ◽  
Oh Hyeok Kwon
Keyword(s):  
Antioxidant Activity ◽  
Poncirus Trifoliata ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Alcoholic Extract ◽  
Total Flavonoid Content ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Antioxidant Effects

Purpose: This study was conducted to investigate the possible use of Ponciri fructus bioconversion extracts as cosmetic ingredients.Method: Total polyphenol, total flavonoid content, and antioxidant effects of Poncirus trifoliata through the ability of diphenylpicrylhydrazyl (DPPH) radical scavenging were used for antioxidant activity determination. The extracts of Poncirus trifoliata were analyzed using an ultraviolet–visible spectrophotometerResults: The total phenolic content was 62.5±4.19 gallic acid equivalent (GAE) mg/g in the case of alcohol extracts and 82.8±2.55 GAE mg/g for other bioconversion extracts. Therefore, total flavonoids content was found to have increased by 32.4%. The total flavonoid content of ethanol extracts was 30.1±3.15 mg/g, whereas that of other bioconversion extracts was 39.5±35.1 mg/g. Therefore, total flavonoids increased by 31.2%. From DPPH radical results, the alcoholic extract increased from 65.5% to 75.5% after bioconversion. Thus, the bioconversion extracts increased by 10% compared with ethanol extracts.Conclusion: Poncirus trifoliata has excellent antioxidant effects, and it showed increased antioxidant activity after the bioconversion, thereby demonstrating its functional bioutility. Therefore, it can be used as an antioxidant material in the cosmetic and food industry.

scholarly journals Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

2021 ◽  
Vol 8 (3) ◽  
pp. 1-4
Author(s):  
Nurlinda Nurlinda ◽  
Virsa Handayani ◽  
Faradiba Abdul Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Caesalpinia Sappan ◽  
Antimicrobial Treatments

AbstractBiancaea sappan  (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

scholarly journals Antioxidant Activity of Dry Birch (Betula Pendula) Leaves Extract

Folia Medica ◽  
2018 ◽  
Vol 60 (4) ◽  
pp. 571-579 ◽  
Author(s):  
Dimitar Penkov ◽  
Velichka Andonova ◽  
Delian Delev ◽  
Ilia Kostadinov ◽  
Margarita Kassarova
Keyword(s):  
Antioxidant Activity ◽  
Betula Pendula ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Albino Rats ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Abstract Background: Betula pendula is widespread in Europe and Asia. It has been used in traditional medicine since ancient times. Birch leaf extracts are known to exhibit a number of pharmacological activities. Antioxidant activity has also been reported. Aim: The aim of this work was to investigate the antioxidant activity of a dry leaf extract from Betula pendula Roth. Materials and methods: The total flavonoid content was determined. Some of the most commonly used methods were applied to evaluate the antioxidant capacity of the extract in vitro and in vivo. The ability of the extract to scavenge DPPH free radicals was evaluated by the method described by Brand-Williams with suitable modifications. ABTS decolorization assay was also applied. The in vivo assay was performed after acute and chronic administration of the extract into white albino rats, in a dose of 100 and 500 mg/kg bw. The antioxidant potential of the plasma was determined using FRAP reagent. Results: A total flavonoid content of 42.5 mg/g was found, expressed as quercetin. The antioxidant activity against ABTS was concentration and time dependent. For example the concentration of 200 μg/ml led to 70.95% – 99.46% scavenging activity. DPPH scavenging activity was found to be about 98% at a concentration of 80 μg/ml. The extract possesses antioxidant potential, comparable with that of Trolox, in acute application. In chronic application, poorer results are observed, probably due to biotransformation and elimination processes. Conclusion: Dried birch leaf extract has a relatively high antioxidant potential and could be used as a natural source of antioxidants.

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

scholarly journals Spectrophotometric determination of sun protective factor (SPF) and antioxidant potential of different solvent extracts of graptophyllum pictum

2019 ◽  
Vol 10 (SPL1) ◽  
Author(s):  
Noor Diyana Ibrahim ◽  
Wan Nur Farihah Che Mustafa ◽  
Mahendran Sekar ◽  
Khor Poh Yen ◽  
Seow Lay Jing
Keyword(s):  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Uv Spectrophotometry ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Natural substances extracted from plants have been gaining attraction as protective agents due to their safety and responsible for multiple biological effects on skins. The present study was to determine the total phenolic and flavonoid contents, Sun Protection Factor (SPF), and antioxidant potential of different solvent extracts of Graptophyllum pictum leaves. The dried powdered leaves were extracted by the cold maceration method, using three different solvents, i.e., methanol, ethanol, and water. The extracted leaves was tested for antioxidant activity using of DPPH (1,1-diphenyl-2-picryl-hydrazyl). Total phenolic content was determined using the Follin-Ciocalteu and for total flavonoid content with used the aluminium chloride. The SPF of all extracts were analyzed by ultraviolet (UV) spectrophotometry. The total phenolic content is the highest in methanol extract (377.61±0.31 µg/ml), and total flavonoid content is the highest in ethanol extract (158.06 ± 0.18 µg/ml). For DPPH free radical scavenging activity, ethanolic extract showed the maximum percentage of inhibition, and for SPF, methanol extract had displayed the highest. The study recommends that methanol is a good solvent for the extraction of sun-protective constituents from G. pictum.

scholarly journals KANDUNGAN SENYAWA FLAVONOID PADA DAUN MELINJO (Gnetum gnemon L.) BERDASARKAN PERBEDAAN TEMPAT TUMBUH

2017 ◽  
Vol 3 (2) ◽  
pp. 142-147
Author(s):  
Mersy T Tanamal ◽  
Pamella Mercy Papilaya ◽  
Alwi Smith
Keyword(s):  
Natural Resources ◽  
Total Flavonoid ◽  
Flavonoid Compound ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Gnetum Gnemon ◽  
The Village

Background: The village of Latuhalat and Kayu Putih village is a place that is on the beach and in the mountains. Potential natural resources are quite a lot of plants melinjo (Gnetum gnemon L). This study aims to determine the total compound of flavonoid content of leaf melinjo (Gnetum gnemon L) based on place difference. Method: The method used in this study using the method of Chang et al. The solvent used for diluting the melinjo extract was methanol solvent, then measured using a UV-VIS spectrophotometer. Result: Result of analysis of flavonoid compound content, Latuhalat Village has average total flavonoid content of13.080% and average total flavonoid content in Kayu Putih Village equal to 17.028%. Kayu Putih Village has more flavonoid compound than Latuhalat Village. Conclusion: Total levels of melinjo leaf flavonoids (Gnetum gnemon L) in Kayu Putih Village were more than total flavonoids of leaf melinjo (Gnetum gnemon L) in Latuhalat Village.

scholarly journals Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Aatika Sikandar ◽  
Mengyue Zhang ◽  
Yuanyuan Wang ◽  
Xiaofeng Zhu ◽  
Xiaoyu Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Penicillium Chrysogenum ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Pharmaceutical Industries

Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.

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