scholarly journals KANDUNGAN SENYAWA FLAVONOID PADA DAUN MELINJO (Gnetum gnemon L.) BERDASARKAN PERBEDAAN TEMPAT TUMBUH

2017 ◽  
Vol 3 (2) ◽  
pp. 142-147
Author(s):  
Mersy T Tanamal ◽  
Pamella Mercy Papilaya ◽  
Alwi Smith
Keyword(s):  
Natural Resources ◽  
Total Flavonoid ◽  
Flavonoid Compound ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Gnetum Gnemon ◽  
The Village

Background: The village of Latuhalat and Kayu Putih village is a place that is on the beach and in the mountains. Potential natural resources are quite a lot of plants melinjo (Gnetum gnemon L). This study aims to determine the total compound of flavonoid content of leaf melinjo (Gnetum gnemon L) based on place difference. Method: The method used in this study using the method of Chang et al. The solvent used for diluting the melinjo extract was methanol solvent, then measured using a UV-VIS spectrophotometer. Result: Result of analysis of flavonoid compound content, Latuhalat Village has average total flavonoid content of13.080% and average total flavonoid content in Kayu Putih Village equal to 17.028%. Kayu Putih Village has more flavonoid compound than Latuhalat Village. Conclusion: Total levels of melinjo leaf flavonoids (Gnetum gnemon L) in Kayu Putih Village were more than total flavonoids of leaf melinjo (Gnetum gnemon L) in Latuhalat Village.

Comparative antioxidant potential and total flavonoids in different extracts of pericarp and pulp of Garcinia cowa Roxb.

2011 ◽  
Vol 1 (4) ◽  
pp. 178-181
Author(s):  
A. K. Chaurasia ◽  
B. J. Gogoi ◽  
P. Chattopadhyay ◽  
H. K. Gogoi ◽  
Lokendra Singh
Keyword(s):  
Antioxidant Activity ◽  
Beta Carotene ◽  
Total Flavonoid ◽  
Fresh Mass ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Garcinia Cowa

The antioxidant and total flavonoid content (TFC) of solvent extracts of pericarpand pulp of Garcinia cowa Roxb. were evaluated. The antioxidant activitywas evaluated using 2, 2 Ã¢â‚¬Âdi phenyl‐1‐picrylhydrazyl (DPPH) free radicalscavenging assay and beta carotene linoleate bleaching ( βCL) assay. Ascorbicacid and butylated hydroxyl toluene (BHT) were used as the standards. Theethyl acetate extract of the pulp showed the highest inhibition (89.5 ±0.81%) of DPPH radical, along with the highest antioxidant activity (75.1 ±0.60%) in βCL assay and the highest total flavonoid content 67.4 ± 1.41 mgcatechin equivalent (CE)/100g fresh mass at 1000ppm concentration.

scholarly journals Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi Kosterm.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Alifni Adha Bakti ◽  
Liling Triyasmono ◽  
Muhammad Ikhwan Rizki
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Natural Resources ◽  
Experimental Research ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Anti Oxidant

ABSTRAK Bahan alam dapat dijadikan bahan obat baru karena mengadung metabolit sekunder. Di Indonesia terdapat lebih kurang 30.000 jenis tumbuh-tumbuhan yang mengandung metabolit sekunder, lebih kurang 7.500 jenis diantaranya termasuk tanaman berkhasiat obat. Salah satunya adalah tanaman kasturi (Mangifera casturi Kosterm.). Tanaman M. casturi merupakan tumbuhan khas Kalimantan Selatan yang hanya dimanfaatkan oleh masyarakat untuk dikonsumsi, tidak untuk pengobatan. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dan aktivitas antioksidan dari ekstrak etanol daun M. casturi. Penelitian ini bersifat non-eksperimental. Sampel yang digunakan adalah daun M. casturi yang berasal dari Kabupaten Banjar, Kalimantan Selatan. Penentuan Kadar flavonoid total dilakukan secara spektrofotometri UV-Vis dengan pereaksi kompleks AlCl3 sedangkan aktivitas antioksidan ditentukan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil penelitian diperoleh kadar flavonoid total ekstrak etanol daun M. casturi sebesar 9,31 ± 0,08 %b/b dan aktivitas antioksidan dengan nilai IC50 sebesar 34,558 ppm sehingga termasuk dalam kategori antioksidan yang sangat aktif. Kata kunci: Antioksidan, DPPH, Flavonoid Total, Mangifera casturi. ABSTRACT Natural resources can be used as the new medicine ingridients because it has second metabolite. In Indonesia, there are more than 30.000 kinds of plants that contain second metabolite, more or less than 7.500 kinds of those are medicinal plants. One of those plants is Kasturi (Mangifera casturi Kosterm.). M. casturi is a typical plant from South Kalimantan that be used as a food not as a medicine. The purpose of this research is to determine the total of flavonoid content and anti-oxidant activity from ethanol extract of M. casturi leaves. This study is a non-experimental research. Sample which used in this research is M. casturi Leaves from Banjar Region, South Kalimantan. The research for total Flavonoid content is done by UV-Vis spectrophotometric with AlCl3 reagent complex while the anti-oxidant activity is determined by DPPH (2,2-difenil-1-pikrilhidrazil) method. The result of this research are extract of M. casturi leaves obtains 9,31 ± 0,08 %b/b of total flavonoid and the antioxidant activity result with IC50 value is 34,558 ppm, so it can be categorized as a very active anti-oxidant. Keywords: Antioxidant, DPPH, Total Flavonoid, Mangifera casturi.

scholarly journals Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

2021 ◽  
Vol 8 (3) ◽  
pp. 1-4
Author(s):  
Nurlinda Nurlinda ◽  
Virsa Handayani ◽  
Faradiba Abdul Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Caesalpinia Sappan ◽  
Antimicrobial Treatments

AbstractBiancaea sappan  (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

scholarly journals Induction of flavonoid biosynthesis by in vitro cultivation of Astragalus glycyphyllos L.

Pharmacia ◽  
2020 ◽  
Vol 67 (2) ◽  
pp. 95-99
Author(s):  
Pavlinka Popova ◽  
Yancho Zarev ◽  
Aleksandar Shkondrov ◽  
Ilina Krasteva ◽  
Iliana Ionkova
Keyword(s):  
Plant Hormones ◽  
Suspension Cultures ◽  
Flavonoid Biosynthesis ◽  
Total Flavonoid ◽  
In Vitro Cultivation ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Establishment of in vitro cultures from Astragalus glycyphyllos, determination of biomass and analysis of total flavonoids, rutin and camelliaside A were performed. To increase flavonoid production various combinations of plant hormones and light/dark regimen were investigated. Suspension cultures with exogenous quercetin were evaluated for possible increase in flavonoid production. Shoots, calli and suspensions were successfully established. Rutin and camelliaside A were proved in highest amount in shoots. Calli, cultivated on modified G48 medium, with double amount of Ca2+ and Mg2+, achieved higher total flavonoid content (2.37 and 2.03 mg/g DW). Suspensions cultures, cultivated on modified G48 medium with 10, 20 and 30 mg/mL quercetin achieved higher total flavonoid content (0.09, 0.10 and 0.13 mg/mg DW). Biotransformation of quercetin to isoquercitrin was achieved. The highest concentration of isoquercitrin (56.73 ng/mg DW) was observed on suspensions cultures cultivated on modified G48 medium, with 20 mg/mL quercetin.

scholarly journals The Existence Of the Betel Leaf (Piper betle L.) is in the Tasawuf Prespective

2019 ◽  
Vol 2 (1) ◽  
pp. 42-46
Author(s):  
Resty Rahmatika
Keyword(s):  
Natural Resources ◽  
Traditional Medicine ◽  
Raw Materials ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Piper Betle ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Betel Leaf

Plants are one of the richest natural resources in Indonesia and contain active chemicals.These plants have the potential to become raw materials for traditional medicine. One of them isbetel plant. The use of Betel leaves, especially in Java, is generally accompanied by specialconditions or criteria, namely: betel leaf which 'meets ros-e'. 'Ros' comes from the Javanese languagewhich means vein, in this case it is a vein of leaves. 'Temu' comes from the Javanese language whichmeans to meet, meet. Betel rose meeting is betel which leaves veins (lateral nerves) meet theposition of the segment; so that the shape is symmetrical. Meanwhile, phytochemical laboratory testresults for total flavonoid content of betel leaves and non-temurose leaves were 53,522 and 43,041ppm. These values include high levels (> 50 ppm) and moderate levels (between> 10 - 50 ppm). Thehigher the level of flavonoids, the more likely the antioxidant potential will be. In theimplementation of riyadhoh, making betel leaves meet rose as a medium as a form of philosophy thatis betel or tell me means ngangsu kaweruh (studying), whereas meet rose can be interpretedas meeting taste.

scholarly journals Antioxidant Effects of Ponciri Fructus Bioconversion Extracts as Cosmetic Ingredients

2021 ◽  
Vol 19 (3) ◽  
pp. 477-485
Author(s):  
Gyu Bin Kong ◽  
Oh Hyeok Kwon
Keyword(s):  
Antioxidant Activity ◽  
Poncirus Trifoliata ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Alcoholic Extract ◽  
Total Flavonoid Content ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Antioxidant Effects

Purpose: This study was conducted to investigate the possible use of Ponciri fructus bioconversion extracts as cosmetic ingredients.Method: Total polyphenol, total flavonoid content, and antioxidant effects of Poncirus trifoliata through the ability of diphenylpicrylhydrazyl (DPPH) radical scavenging were used for antioxidant activity determination. The extracts of Poncirus trifoliata were analyzed using an ultraviolet–visible spectrophotometerResults: The total phenolic content was 62.5±4.19 gallic acid equivalent (GAE) mg/g in the case of alcohol extracts and 82.8±2.55 GAE mg/g for other bioconversion extracts. Therefore, total flavonoids content was found to have increased by 32.4%. The total flavonoid content of ethanol extracts was 30.1±3.15 mg/g, whereas that of other bioconversion extracts was 39.5±35.1 mg/g. Therefore, total flavonoids increased by 31.2%. From DPPH radical results, the alcoholic extract increased from 65.5% to 75.5% after bioconversion. Thus, the bioconversion extracts increased by 10% compared with ethanol extracts.Conclusion: Poncirus trifoliata has excellent antioxidant effects, and it showed increased antioxidant activity after the bioconversion, thereby demonstrating its functional bioutility. Therefore, it can be used as an antioxidant material in the cosmetic and food industry.

scholarly journals Effect of Pectin Biodegradation with Aspergillus niger on Total Flavonoid Content of Citrus limon L.)

2019 ◽  
Vol 4 (2) ◽  
pp. 194-203
Author(s):  
Muhammad Yusuf Abduh ◽  
Wahyu Zuliansyah ◽  
Lestari Aprina ◽  
Natalie Arazella
Keyword(s):  
Aspergillus Niger ◽  
Dry Weight ◽  
Total Flavonoid ◽  
Flavonoid Compound ◽  
Citrus Limon ◽  
Lower Grade ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Cultivation Time ◽  
Lemon Peel

Lemon peel and leaf  as well as lower grade of lemon contain a considerable amount of flavonoid which can be extracted to produce a valuable product. The presence of pectin in lemon hinders the extraction of flavonoid compound. This research was conducted to examine the effect of pectin biodegradation of lemon leaf, peel, and lower grade fruit using solid-state fermentation towards the yield of pectin, crude flavonoid, and total flavonoid content. Fermentation was carried out with the help of Aspergillus niger at 30℃ for 3, 5, 7, and 9 days of cultivation time. Pectin was extracted from the fermentation broth using citric acid. Crude flavonoid was extracted using maceration and stirred about 500 rpm at 50℃ for 40 minutes. Total flavonoid content was analyzed using a spectrophotometer. The optimum cultivation time of Aspergillus niger with leaf and peel was 9 days with a pectin yield from lemon leaf was 0.43% dry weight and the pectin yield from lemon peel was 0.04% dry weight. As for the lower grade fruit, a cultivation time for 7 days showed an optimal result with a pectin yield of 0.08% dry weight. The total flavonoid content in the leaf increased up to 94.3% (1.06 mg of the quercetin equivalents per g of substrate) whereas the total flavonoid content in the peel and lower grade fruit increased up to 42% (0.12 mg of the quercetin equivalents per g of substrate) and 48% (0.021 mg of the quercetin equivalents per g of substrate), respectively. These results highlighted the potential valorization of Citrus limon L. as an alternative source for producing flavonoid in food and pharmaceutical industry.  

scholarly journals ANALISIS KORELASI ANTARA FLAVONOID TOTAL DENGAN AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI DAUN GEDI HIJAU (Abelmoschus manihot L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 591
Author(s):  
Josua A.T. Suoth ◽  
Sri Sudewi ◽  
Defny S. Wewengkang
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content

One chemichal component that is often reported to have pharmacological activity against treatment or prevention of disease is flavonoids which can be found in gedi hijau leaves. Staphylococcus aureus is the main cause of infection in humans which can cause various severe infections. This study aims to determine the total flavonoids content, minimum inhibitory concentration (MIC). To determine the correlation between total flavonoids and antibacterial activity of extract and fractions (Abelmoschus manihot L.), The method of this study was done by obtaining the results of total flavonoid content, testing the antibacterial activity by dilution using a positive control of ciprofloxacin. The results showed the highest total flavonoid content in ethyl acetate fraction, and the best antibacterial activity in ethyl acetate fraction. Correlation between total flavonoid content from extract and fraction (Abelmoschus manihot L.) with antibacterial activity against Staphylococcus aureus bacteria on ethyl acetate fraction, n-hexane fraction, and ethanol extract at a concentration of 1000 µg / mL had good values with kneeling significance values according to 0.012 (p <0.05), 0.034 (p <0.05), and 0.023 (p <0.05) with total flavonoid content affecting how much antibacterial activity is 40% for ethyl acetate fraction, 35% fraction n -heksane and 33% ethanol extract. Keywords         : Antibacterial, Total Flavonoids, Staphylococcus aureus, Abelmoschus manihot L. ABSTRAK Salah satu komponen kimia yang sering dilaporkan memiliki aktivitas farmakologi terhadap pengobatan atau pencegahan penyakit adalah flavonoid yang dapat ditemukan pada daun gedi hijau. Staphylococcus aureus merupakan kuman penyebab utama infeksi pada manusia yang dapat menyebabkan berbagai infeksi berat. Penelitian ini bertujuan menentukan kandungan flavonoid total, aktivitas antibakteri dan korelasi antara flavonoid total dengan aktivitas antibakteri dari ekstrak dan fraksi (Abelmoschus manihot L.). Metode penelitian ini dilakukan dengan mendapatkan hasil kandungan flavonoid total, uji aktivitas antibakteri dengan cara dilusi menggunakan kontrol positif ciprofloxacin. Hasil penelitian didapatkan kandungan flavonoid total tertinggi pada fraksi etil asetat, dan aktivitas antibakteri paling bagus pada fraksi etil asetat. Korelasi antara kandungan flavonoid total dari ekstrak dan fraksi (Abelmoschus manihot L.) dengan aktivitas antibakteri terhadap bakteri Staphylococcus aureus pada fraksi etil asetat, fraksi n-heksane, dan ekstrak etanol pada konsentrasi 1000 µg/mL memiliki nilai yang baik baik dengan nilai signifikansi bertutut-turut 0,012 (p <0,05), 0,034  (p <0,05), dan 0,023 (p <0,05) dengan kandungan flavonoid total mempengaruhi seberapa besar aktivitas antibakteri yaitu 40% untuk fraksi etil asetat, 35% fraksi n-heksane dan 33% ekstrak etanol.Kata kunci          : Antibakteri, Flavonoid Total, Staphylococcus aureus, Abelmoschus manihot L.

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

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