PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT STUDIES OF TERMINALIA BELLIRICA HYDROALCOHOLIC EXTRACT

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

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Evaluation of Phyto Chemical Biochemical and In Vitro Antioxidant Potential of Angelica Glauca Grown at High Altitude Areas of Western Himalayas

Defence Life Science Journal ◽

10.14429/dlsj.6.15770 ◽

2021 ◽

Vol 6 (2) ◽

pp. 117-121

Author(s):

Swati Arya ◽

H.K. Pandey ◽

A. Singh ◽

H.S. Meena ◽

M. Bala

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Biochemical Parameter ◽

Antioxidant Potential ◽

Phytochemical Analysis ◽

Root Extract ◽

Western Himalayas ◽

Hydroalcoholic Extract ◽

Angelica Glauca

Angelica glauca Edgew is an important medicinal and aromatic herb (family Apiaceae). The roots of A. glauca commonly used as spices by local peoples. In the present study, the phytochemical constituents, biochemical parameters and, in-vitro antioxidant activity of A. glauca roots collected from the Himalayan region have been studied. For preliminary phytochemical analysis, the hydroalcoholic and aqueous root extract of Angelica glauca were screened for the presence of carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The biochemical parameter and in-vitro antioxidant potential of Angelica glauca were analysed by using standard methods. Phytochemical analysis of extract showed major classes of phytochemicals constituents such as carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The results from the current study demonstrated that A.glauca roots contained carbohydrate (21±0.72 %), crude protein (12.7±0.31 %), total ash (3.86±0.034 %), dietary fiber (18.9±0.14 %), total fat (4.5±0.38 %) and ascorbic acid (68.5±0.19mg/100g). The hydroalcoholic extract showed the highest quantity of total phenol, total flavonoids, and total tannin content compared to aqueous extract. The hydroalcoholic extract exhibited high DPPH radical scavenging activity (IC50=68.1±0.34 µg/ml). The result showed that A.glauca roots have high nutritional and antioxidant potential. Hence the plant can be used as a nutraceutical and natural antioxidant.

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Phytochemical analysis, antioxidant, antidiabetic and anti-inflammatory activity of bark of Gardenia latifolia

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1.2196 ◽

2019 ◽

Vol 9 (1) ◽

pp. 141-145

Author(s):

Farheen Ansari ◽

Salaj Khare ◽

B. K. Dubey ◽

Amit Joshi ◽

Amit Jain ◽

...

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Inflammatory Activity ◽

Phytochemical Analysis ◽

Dpph Radical ◽

Hydroalcoholic Extract ◽

Radical Method ◽

Anti Inflammatory

Gardenia latifolia (Rubiaceae) is commonly known as Indian boxwood or Ceylon boxwood, is a densely foliaceous small tree that occurs throughout the greater parts of Indian common in deciduous forests along the streams. The stem bark and fruits are reported to be used in the treatment of various ailments such as snake bite, skin diseases, stomach pains, caries in humans and ephemeral fever in live stocks. Many beneficial medicinal products are used to treat various serious diseases and disorders like diabetes, cancer and cardiovascular diseases without side effects. Hence our study focused to investigate the phytochemical analysis, quantification of bioactive compounds, in vitro free radical scavenging activity (DPPH radical method), anti-inflammatory activity (Carrageenan induced hind paw oedema ), in vitro anti-diabetic activity by enzyme inhibition activity of bark of Gardenia latifolia (hydroalcoholic extract) which has boundless medicinal properties. The results of this study showed the evidence that the extracts when tested for their phytochemicals and free radical scavenging activity were found to have considerable antioxidant potential. This plant also exhibit better in vitro enzyme inhibitory activity and anti-inflammatory activity. The results of this study indicate that the hydroalcoholic extract of Gardenia latifolia bark has significant pharmacological properties. Keywords: Gardenia latifolia, Phytochemical analysis, DPPH radical method, Anti-inflammatory activity, Anti-diabetic activity

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Atividade antimicrobiana e análise fitoquímica preliminar do extrato vegetal de alho no controle de fungos fitopatogênicos

Revista Verde de Agroecologia e Desenvolvimento Sustentável ◽

10.18378/rvads.v12i1.4406 ◽

2017 ◽

Vol 12 (1) ◽

pp. 161

Author(s):

Nadine Cunha Costa ◽

Aloisio Freitas Chagas Junior ◽

Antônio Carlos Costa Ramos ◽

Layssah Passos Soares ◽

Gessiel Newton Scheidt

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Fungi ◽

Alternative Methods ◽

Phytochemical Analysis ◽

Curvularia Lunata ◽

Fusarium Subglutinans ◽

Hydroalcoholic Extract ◽

Plant Disease Control ◽

Phytochemical Profile

As potencialidades de utilização de métodos alternativos no controle de doenças de plantas são incessantemente estudadas e avaliadas por vários pesquisadores no mundo inteiro, com o objetivo de minimizar o impacto ambiental através da utilização de produtos naturais. Este trabalho teve como objetivo traçar o perfil fitoquímico preliminar verificando a atividade antimicrobiana in vitro do extrato hidroalcóolico de Allium sativum L. contra Bipolaris spp., Curvularia lunata e Fusarium subglutinans através do mecanismo de pulverização na Torre de Potter. Observou-se na análise fitoquímica a presença de taninos hidrolisáveis, saponinas e flavonóides. O extrato possuiu atividade antimicrobiana sobre todos os fungos fitopatogênicos testados, com destaque para os fungos Curvularia lunata e Fusarium subglutinans nas concentrações de 40 a 50%, no entanto, o fungo Bipolaris spp. mostrou-se mais resistente com sensibilidade apenas na concentração máxima de 50%.Antimicrobial activity and analysis phytochemical preliminary extract vegetable garlic in fungi phytopathogenic controlAbstract: The potential use of alternative methods in plant disease control are constantly studied and evaluated by several researchers worldwide with the aim of minimizing the environmental impact through the use of natural products. This study aimed to trace the preliminary phytochemical profile and check the in vitro antimicrobial activity of garlic hydroalcoholic extract in different concentrations against Bipolaris spp., Curvularia lunata and Fusarium subglutinans through the spray mechanism in the Potter Tower. It was observed in the presence of phytochemical analysis hydrolysable tannins, saponins and flavonoids. The extract possessed antimicrobial activity against all pathogenic fungi tested, especially the fungi Curvularia lunata and Fusarium subglutinans in concentrations of 40 to 50%, however, the fungus Bipolaris spp. it proved to be tougher with sensitivity only at the maximum concentration of 50%.

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QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

FLORA AND FAUNA ◽

10.33451/florafauna.v24i2pp350-354 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

GITA MISHRA ◽

HEMESHWER KUMAR CHANDRA ◽

NISHA SAHU ◽

SATENDRA KUMAR NIRALA ◽

MONIKA BHADAURIA

Keyword(s):

Reducing Sugars ◽

Antioxidant Properties ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Qualitative And Quantitative ◽

The Family ◽

Ethanolic Extracts ◽

Antioxidant Evaluation ◽

In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

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Phytochemical Analysis and In Vitro Antibacterial Activity of Soymida febrifuga (Roxb.) Juss. and Hemidesmus indicus (L.)

Indian Journal Of Applied Research ◽

10.15373/2249555x/dec2013/14 ◽

2011 ◽

Vol 3 (12) ◽

pp. 57-59 ◽

Cited By ~ 1

Author(s):

K. Riazunnisa K. Riazunnisa ◽

◽

U. Adilakshmamma U. Adilakshmamma ◽

C. Habeeb khadri

Keyword(s):

Antibacterial Activity ◽

Phytochemical Analysis ◽

Hemidesmus Indicus ◽

In Vitro Antibacterial Activity

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Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

International Journal of Pharmaceutical and Phytopharmacological Research ◽

10.24896/eijppr.2016616 ◽

2017 ◽

Vol 6 (1) ◽

pp. 34

Author(s):

Michael Russelle Alvarez ◽

Paolo Robert Bueno ◽

Raymond Oliver Cruz ◽

Richard Macapulay ◽

Francis Jayson Vallesfin ◽

...

Keyword(s):

Crude Extract ◽

Carica Papaya ◽

Uv Light ◽

Digestive Enzyme ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Salivary Amylase ◽

Garcinia Mangostana ◽

Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

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Preliminary Phytochemical Analysis and Anti-Bacterial Activity of Cucurbita Maxima Seed Oil – An In Vitro Study

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.sp1.405 ◽

2020 ◽

Vol 12 (sp1) ◽

Keyword(s):

Seed Oil ◽

In Vitro Study ◽

Bacterial Activity ◽

Vitro Study ◽

Phytochemical Analysis ◽

Cucurbita Maxima

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Tamarix articulata (T. articulata) - An Important Halophytic Medicinal Plant with Potential Pharmacological Properties

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190318120103 ◽

2019 ◽

Vol 20 (4) ◽

pp. 285-292 ◽

Cited By ~ 5

Author(s):

Abdullah M. Alnuqaydan ◽

Bilal Rah

Keyword(s):

Medicinal Plant ◽

Biological Activities ◽

Wide Spectrum ◽

Biological Properties ◽

In Vivo Model ◽

Drug Candidate ◽

Phytochemical Analysis ◽

Pharmacological Properties

Background:Tamarix Articulata (T. articulata), commonly known as Tamarisk or Athal in Arabic region, belongs to the Tamaricaece species. It is an important halophytic medicinal plant and a good source of polyphenolic phytochemical(s). In traditional medicines, T. articulata extract is commonly used, either singly or in combination with other plant extracts against different ailments since ancient times.Methods:Electronic database survey via Pubmed, Google Scholar, Researchgate, Scopus and Science Direct were used to review the scientific inputs until October 2018, by searching appropriate keywords. Literature related to pharmacological activities of T. articulata, Tamarix species, phytochemical analysis of T. articulata, biological activities of T. articulata extracts. All of these terms were used to search the scientific literature associated with T. articulata; the dosage of extract, route of administration, extract type, and in-vitro and in-vivo model.Results:Numerous reports revealed that T. articulata contains a wide spectrum of phytochemical(s), which enables it to have a wide window of biological properties. Owing to the presence of high content of phytochemical compounds like polyphenolics and flavonoids, T. articulata is a potential source of antioxidant, anti-inflammatory and antiproliferative properties. In view of these pharmacological properties, T. articulata could be a potential drug candidate to treat various clinical conditions including cancer in the near future.Conclusion:In this review, the spectrum of phytochemical(s) has been summarized for their pharmacological properties and the mechanisms of action, and the possible potential therapeutic applications of this plant against various diseases discussed.

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