Prevalence and antibiotic susceptibility of ear pathogens isolated from patients in Tripoli, north of Lebanon

Background. Urinary tract infection (UTI) is a severe public health problem. However, infected patients are usually treated empirically without preceding culture or antibiotics susceptibility testing, which may increase the antibiotic resistance level. The aim of this study is to determine the prevalence and antibiotic susceptibility patterns of common bacterial uropathogens isolated in Akkar governorate, North Lebanon. Methods. Spot midstream from urine samples from 9662 patients presenting UTI symptoms who came to Youssef Hospital Center located in Akkar governorate, were collected in sterile plastic cups. Culture, identification and antibiotic susceptibility testing were performed through conventional tools according to the manufacturer’s recommended procedures and the recommendations of the European Committee on Antimicrobial Susceptibility Testing. Results. Overall, a total of 1009 bacterial uropathogens were isolated. Escherichia coli was predominant and represented 72.5% of all isolates , followed by Klebsiella pneumoniae (8.2%), Enterococcus spp. (5.5%), Pseudomonas aeruginosa (4.5%), Proteus spp. (3%), Enterobacter spp. (2%), Staphylococcus aureus (2%), Streptococcus agalactiae (1.6%), Staphylococcus saprophyticus (0.4%), Acinetobacter baumannii (0.2%) and Providencia rettgeri (0.1%). Moreover, the mean antibiotic resistance rates of isolates was relatively high, but similar to previous investigations reported in our country. Conclusion. To our knowledge, this is the first investigation reporting epidemiological data regarding the prevalence and antibiotic susceptibility patterns of uropathogens isolated from patients in Akkar governorate. Our data indicated the urgent need of a strategic plan to tackle antibiotic resistance, particularly in deprived regions with poor healthcare structures such as Akkar governorate. DOI: http://dx.doi.org/10.3823/801

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Prevalence and antibiotic susceptibility patterns of bacteria causing urinary tract infections in Youssef Hospital Center: first report from Akkar governorate, North of Lebanon

The International Arabic Journal of Antimicrobial Agents ◽

10.3823/0802 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Monzer Hamze ◽

Marwan Osman ◽

Hassan Mallat ◽

Elie Bou Raad

Keyword(s):

Antibiotic Resistance ◽

Urinary Tract ◽

Antibiotic Susceptibility ◽

Susceptibility Testing ◽

Published Data ◽

Resistance Level ◽

Antibiotic Susceptibility Patterns ◽

Tract Infections ◽

Susceptibility Patterns ◽

Hospital Center

Background. Urinary tract infection (UTI) is common infection feature worldwide. Infected patients are usually treated empirically treated without culture or antibiotics susceptibility testing, and which may lead to increase antibiotic resistance level. This study aims to determine the prevalence and antibiotic susceptibility patterns of common uropathogenic bacteria isolated at Youssef Hospital Center, Akkar governorate, North of Lebanon. Methods. Spot midstream of urine samples from 9662 patients with UTI symptoms who came for medical investigation to Youssef Hospital Center located in Akkar governorate. Urine specimens were collected in sterile plastic bottles. Culture, identification and antibiotic susceptibility testing were performed using conventional tools according to the recommendations of the European Committee on Antimicrobial Susceptibility Testing. Results. A total of 1009 bacterial uropathogens were isolated. Escherichia coli was most presented (72.5%) of all isolates, followed by Klebsiella pneumoniae (8.2%), Enterococcus spp. (5.5%), Pseudomonas aeruginosa (4.5%), Proteus spp. (3%), Enterobacter spp. (2%), Staphylococcus aureus (2%), Streptococcus agalactiae (1.6%), Staphylococcus saprophyticus (0.4%), Acinetobacter baumannii(0.2%) and Providencia rettgeri (0.1%). Moreover, the mean antibiotic resistance rates of isolates was relatively high, but comparable to previously published data in Lebanon. Conclusion. To the best of our knowledge, this is the first investigation reporting epidemiological data regarding the prevalence and antibiotic susceptibility patterns of bacterial uropathogens isolated from patients in the Akkar governorate. Our data indicated the urgent need of a strategic plan to tackle antibiotic resistance, particularly in deprived regions with poor healthcare centers. Keywords: Uropathogens, Epidemiology, Antibiotic susceptibility, Risk factors, Akkargovernorate, North Lebanon.

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One year of Neisseria gonorrhoeae isolates in Sweden: the prevalence study of antibiotic susceptibility shows relation to the geographic area of exposure

International Journal of STD & AIDS ◽

10.1258/0956462021924730 ◽

2002 ◽

Vol 13 (2) ◽

pp. 109-114 ◽

Cited By ~ 21

Author(s):

Torsten Berglund ◽

Magnus Unemo ◽

Per Olcén ◽

Johan Giesecke ◽

Hans Fredlund

Keyword(s):

Neisseria Gonorrhoeae ◽

Antibiotic Susceptibility ◽

Epidemiological Data ◽

Geographic Area ◽

Risk Of Infection ◽

First Choice ◽

Choice Of Treatment ◽

Antibiotic Susceptibility Patterns ◽

One Year ◽

Susceptibility Patterns

The aim of this study was to compare epidemiological data with antibiotic susceptibility patterns, so as to characterize the risk of infection with a highly resistant Neisseria gonorrhoeae strain. N. gonorrhoeae strains isolated in Sweden from February 1998 through January 1999 were tested for antibiotic susceptibility. Epidemiological data were received from each clinician reporting a case of gonorrhoea and these data were linked to the N. gonorrhoeae strains. A total of 348 N. gonorrhoeae isolates, representing 89% of all Swedish cases diagnosed during the 12-month period, were tested for antibiotic susceptibility. Of all isolates, 24% were β-lactamase-producing, and 18% had decreased susceptibility to ciprofloxacin (MIC>0.064 mg/l). All isolates were fully susceptible to ceftriaxone and spectinomycin. More than 99% of the isolates were fully susceptible to azithromycin. The antibiotic susceptibility varied with the places where patients were exposed to infection. When exposed in Asia, 63% of the isolates showed reduced susceptibility to ciprofloxacin, compared with 0-8.5% of the isolates from patients exposed in other places (RR=8.5, P<0.001). Ciprofloxacin cannot be recommended as the first choice of treatment if the place of exposure was in Asia.

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Comparison of Antibiotic Susceptibility Profiles and Molecular Typing Patterns of Clinical and Environmental Salmonella enterica Serotype Newport

Journal of Food Protection ◽

10.4315/0362-028x-69.4.749 ◽

2006 ◽

Vol 69 (4) ◽

pp. 749-756 ◽

Cited By ~ 12

Author(s):

APARNA TATAVARTHY ◽

KEALY PEAK ◽

WILLIAM VEGUILLA ◽

FRANK REEVES ◽

ANDREW CANNONS ◽

...

Keyword(s):

Antibiotic Resistance ◽

Molecular Typing ◽

Salmonella Enterica ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Source Tracking ◽

Resistance Testing ◽

Salmonella Newport ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

The genus Salmonella is composed of more than 2,400 serotypes, many of which cause enteric diseases in humans and animals. Several Salmonella serotypes are multidrug resistant, and there is evidence of the clonal spread of these strains from animals to humans. Salmonella enterica serotype Newport is one of the serotypes that increasingly present a multidrug-resistant phenotype. Source tracking and antibiotic resistance testing are important considerations for identifying the outbreak strain. The first goal of this study was to examine the antibiotic susceptibility patterns of clinical and environmental Salmonella Newport isolates from various geographic locations and to compare the discriminatory ability of two DNA fingerprinting techniques. The second goal was to determine whether the antibiotic resistance profiles and typing patterns correlated. Thirty Salmonella Newport isolates, including environmental and human clinical strains, were subjected to pulsed-field gel electrophoresis (PFGE), ribotyping, and antibiotic susceptibility testing. Eighty percent of the isolates showed total or intermediate resistance to one or more drugs; 75% of the isolates were multidrug resistant. Ribotyping with the EcoRI enzyme and PFGE with the XbaI enzyme each divided the isolates into 14 groups. Cluster analysis based on antibiotic susceptibility patterns generated 23 profiles. The susceptible and resistant isolates were not differentiated on the basis of either of the molecular typing techniques. Hence, no correlation was observed between the antibiotic resistance profiles and the DNA subtyping patterns. In conclusion, ribotyping is as discriminatory as PFGE and, when used in combination with antibiotic resistance profiles, provides a powerful tool for the source tracking of Salmonella Newport.

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Antibiotic Susceptibility Patterns of Bacteria Isolated from Sachet-packaged Water Sold in Uyo Metropolis, Akwa Ibom State, Nigeria

International Journal of Pathogen Research ◽

10.9734/ijpr/2019/v3i130085 ◽

2019 ◽

pp. 1-11

Author(s):

Uduak S. Umoessien ◽

Ukponobong E. Antia ◽

Mary Christopher ◽

Etanguno E. Owowo

Keyword(s):

Antibiotic Susceptibility ◽

World Health ◽

Diffusion Method ◽

Bacillus Sp ◽

Routine Monitoring ◽

Antibiotic Susceptibility Patterns ◽

Antibiotics Susceptibility ◽

Health Organization ◽

Susceptibility Patterns ◽

Sachet Water

Aims: This study was aimed at determining the antibiotic susceptibility patterns of bacteria isolated from sachet water sold in Uyo metropolis, Akwa Ibom State, Nigeria. Study Design: Sachet water was randomly sampled in Uyo Metropolis. Place and Duration of Study: Department of Microbiology, Akwa Ibom State University, Nigeria, between June and November 2018. Methodology: Six different brands of sachets water sold and consumed in Uyo metropolis were studied for their physical and microbiological qualities. Thirty (30) sachets water from the six (6) different brands respectively, were serially diluted and cultured on Nutrient agar, Eosin Methylene Blue agar, MacConkey agar and Salmonella-Shigella agar, while Muller Hinton agar was used for sensitivity test. Suspensions of purified isolates were standardized with 0.5 McFarland turbidity standard and were subjected to antibiotics susceptibility testing using Agar Diffusion method. Results: The bacterial counts obtained ranged from 2.0 x 101 cfu/ml to 1.34 x 102 cfu/ml. Species isolated from the samples analysed included: Klebsiella sp., Escherichia sp., Staphylococcus sp., Salmonella sp., Pseudomonas sp., Citrobacter sp., Bacillus sp. Bacillus sp. Was susceptible to all the antibiotics tested against it except streptomycin while Staphylococcus sp was resistant to gentamicine and ampiclox but susceptible to other antibiotics. All the gram negative isolates were susceptible to tarivia and peflacine but completely resistant to nalidixic acid. Klebsiella sp. was most resistant (70%) of all the isolates, these was closely followed bt Escherichia sp. and Salmonella sp. at 60% resistance. Some of the sachet water brands from bacteriological standpoints did not meet the World Health Organization Standard for portable water. Conclusion: This study indicted sub-standard packaged waters as a vehicle for the spread of antibiotic resistant bacterial pathogens, and this poses a high risk to public health. Hence, routine monitoring of producers of sachet water should been enforced.

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Epidemiology and Antibiotic Susceptibility Patterns of Carbapenem Resistant Gram Negative Bacteria Isolated from Two Tertiary Care Hospitals in North Lebanon

The International Arabic Journal of Antimicrobial Agents ◽

10.3823/823 ◽

2018 ◽

Vol 8 (2) ◽

Author(s):

Monzer Hamze

Keyword(s):

Antibiotic Susceptibility ◽

Susceptibility Testing ◽

Tertiary Care ◽

Resistant Bacteria ◽

Major Public Health Problem ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Carbapenem Resistant ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

Background. Antimicrobial resistance is a major public health problem worldwide. Numerous epidemiological studies reported that Lebanon is affected with high levels of antibiotic resistance. The aim of this study is to determine the prevalence and antibiotic susceptibility patterns of carbapenem resistant Gram negative bacteria in North Lebanon during the period 2015-2017. Methods. Carbapenem resistant Gram negative bacteria were isolated from patients referring to Nini hospital and Youssef hospital center. Identification and antibiotic susceptibility testing were performed through conventional tools according to the manufacturer’s recommended procedures and the recommendations of the European Committee on Antimicrobial Susceptibility Testing, respectively. Results. Overall, a total of 290 carbapenem resistant Gram negative bacteria were isolated. Escherichia coli was predominant and represented 39.3% of all isolates, followed by Pseudomonas aeruginosa (24.8%), Acinetobacter baumannii (22.8%), Klebsiella spp. (8.6%), Enterobacter spp. (6.6%), Pantoea spp. (1%), and Proteus vulgaris (0.3%). Our findings showed an alarming increase in the prevalence of carbapenem resistant bacteria every year. On the other hand, colistin, tigecycline, amikacin and fosfomycin remain the most effective agents against carbapenem resistant Gram negative bacteria. Conclusion. This study provided important new laboratory data that could support specialists in infectious diseases in North Lebanon to take the appropriate decision in the treatment of patients at risk for infections with carbapenem resistant Gram negative germs.

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Bacteriological Profiles and Antibiotic Susceptibility Patterns in Complicated Bacterial Infections of the Ears, Nose and Throat

Revista de Chimie ◽

10.37358/rc.19.12.7770 ◽

2020 ◽

Vol 70 (12) ◽

pp. 4425-4431

Keyword(s):

Antibiotic Resistance ◽

Surgical Treatment ◽

Antibiotic Susceptibility ◽

Bacterial Infections ◽

Middle Ear Cholesteatoma ◽

Life Threatening ◽

Antibiotic Susceptibility Patterns ◽

Cervical Ganglia ◽

Susceptibility Patterns ◽

Nose And Throat

The objective of this study was to identify and map bacteriological profiles and antibiotic susceptibility patterns of complicated bacterial infections in the otorhinolaryngological (ENT) spectrum. Antibiotic resistance was another goal of our study. We defined complicated bacterial infections as: acute bacterial pathology of the ears, nose and throat with complications that required immediate surgical approach, chronic diseases of the ears, nose and throat that required surgical treatment due to ongoing bacterial infections, middle ear cholesteatoma with complications, complicated suppurated tonsillitis in children, intratonsillar abscess, parapharyngeal space abscess, cellulitis of the neck, face and/or involving cervical ganglia and neoplasms of the pharynx or larynx with an acute ongoing bacterial superinfection. The study was carried out over a period of 12 months, on a total number of 82 patients (children and adults), admitted in our department for the complicated bacterial diseases listed above. For the ENT spectrum, we uncovered that more than 50% of complicated bacterial infections were caused by 4 pathogens: Pseudomonas species, Staphylococcus aureus non-MRSA and MRSA species and Streptococcus species (other than Beta-haemolytic Group A Streptococci and Streptococcus pneumoniae). Almost two thirds of all complicated bacterial infections are complicated cases of sinusitis or mastoiditis that require immediate surgical treatment due to possible life-threatening complications. Keywords: complicated bacterial infection, bacteriological profiles, antibiotic resistance, bacterial culture, bacterial antibiotic susceptibility

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Exposure to Biocides Leads to Ampicillin Resistance in Clinically Isolated Enterococcus faecalis: A Preliminary Study

10.21203/rs.3.rs-22769/v1 ◽

2020 ◽

Author(s):

Amirah Fatihah Md Nor ◽

Nur Ainaa Nabihah Mohd Ghazali ◽

Rosni Ibrahim ◽

Siti Khadijah Adam ◽

Azmiza Syawani Jasni

Keyword(s):

Antibiotic Resistance ◽

Enterococcus Faecalis ◽

Antibiotic Susceptibility ◽

Pcr Amplification ◽

Clinical Settings ◽

Subinhibitory Concentration ◽

Ampicillin Resistance ◽

Nosocomial Pathogens ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

Abstract BackgroundAntibiotic resistance is one of the most urgent threats to the public health globally. The extensive use of biocides in clinical settings poses risk for emergence and spread of antibiotic resistance among nosocomial pathogens. This study aimed to investigate the effect of biocides exposure on the antibiotic susceptibility patterns, morphology and ultrastructure of clinically isolated Enterococcus faecalis.MethodsThe antibiotic susceptibility patterns of E. faecalis following biocides exposure were studied using disk diffusion and broth microdilution methods. Polymerase chain reaction (PCR) amplification was performed to confirm the ampicillin resistance genes. The effect on the morphological and ultrastructural of E. faecalis was characterized using transmission and scanning electron microscopy. All descriptive data from this study were presented as percentages.ResultsExposure to subinhibitory concentration of hydrogen peroxide increased the MIC of E. faecalis EF2 and developed resistance to ampicillin. However, the exposure did not exhibit any effects to the ultrastructure and morphology of E. faecalis. Molecular analysis of E. faecalis EF2 and ampicillin resistant EF2 which was referred as EF2r in this study showed that both isolates contained pbp4 gene. Sequence analysis revealed that there was no mutation detected within the pbp4 gene thus suggesting that ampicillin resistance observed following exposure to subinhibition concentration of biocides did not cause any mutation within the pbp4 gene of E. faecalis EF2r.ConclusionsThis study demonstrated that exposure to subinhibitory concentration of biocides leads to reduced antibiotic susceptibility of the clinical E. faecalis. This investigation provides an insight into the event that could lead to the development and possible dissemination of antibiotic resistance determinants among nosocomial pathogens.

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