Chemical Composition, Antibacterial and in vitro Anti-Inflammatory Potentials of Essential Oils from Different Plant Parts of Moringa oleifera Lam

Background: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted. Objective: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol. Material and Methods: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME). Results: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and β-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 μg/mL), which is lower than standard ascorbic acid (IC50 2.58 μg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 μg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 μg/mL, ME 36.44 μg/mL, and sodium diclofenac 22.76 μg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil. Conclusion: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.

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Bioaccessibility, antioxidant activity and modulation effect on gut microbiota of bioactive compounds from Moringa oleifera Lam. leaves during digestion and fermentation in vitro

Food & Function ◽

10.1039/c9fo00793h ◽

2019 ◽

Vol 10 (8) ◽

pp. 5070-5079 ◽

Cited By ~ 9

Author(s):

Zuman Dou ◽

Chun Chen ◽

Xiong Fu

Keyword(s):

Antioxidant Activity ◽

Gut Microbiota ◽

Bioactive Compounds ◽

Moringa Oleifera ◽

Modulation Effect ◽

Gastrointestinal Digestion ◽

Colonic Fermentation ◽

Moringa Oleifera Lam

This study aims to investigate the bioaccessibility, bioactivity and gut microbiota modulation effect of Moringa oleifera Lam. leaves after in vitro gastrointestinal digestion and colonic fermentation.

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Chemical composition and anti-inflammatory activities of the essential oils fromAcacia mearnsiide Wild

Natural Product Research ◽

10.1080/14786419.2014.983504 ◽

2014 ◽

Vol 29 (12) ◽

pp. 1184-1188 ◽

Cited By ~ 8

Author(s):

Opeyemi N. Avoseh ◽

Ope-oluwa O. Oyedeji ◽

Kayode Aremu ◽

Benedicta N. Nkeh-Chungag ◽

Sandile P. Songca ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Anti Inflammatory

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Suppression of tumor necrosis factor-alpha-induced neutrophil adherence responses by essential oils

Mediators of Inflammation ◽

10.1080/09629350310001633342 ◽

2003 ◽

Vol 12 (6) ◽

pp. 323-328 ◽

Cited By ~ 39

Author(s):

Shigeru Abe ◽

Naho Maruyama ◽

Kazumi Hayama ◽

Hiroko Ishibashi ◽

Shigeharu Inoue ◽

...

Keyword(s):

Essential Oils ◽

Tumor Necrosis ◽

Neutrophil Activation ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Neutrophil Adherence ◽

Factor Alpha ◽

Anti Inflammatory ◽

Necrosis Factor

Background:In aromatherapy, essential oils are used as anti-inflammatory remedies, but experimental studies on their action mechanisms are very limited.Aims:To assess their anti-inflammatory activities, effects of essential oils on neutrophil activation were examinedin vitro.Methods:Neutrophil activation was measured by tumor necrosis factor-alpha (TNF-α)-induced adherence reaction of human peripheral neutrophils.Results:All essential oils tested at 0.1% concentration suppressed TNF-α-induced neutrophil adherence, and, in particular, lemongrass, geranium and spearmint oils clearly lowered the reaction even at 0.0125%. Similar inhibitory activities for the neutrophil adherence were obtained by their major constituent terpenoids: citral, geraniol, citronellol and carvone. In contrast, very popular essential oils, tea tree oil and lavender oil, did not display the inhibitory activity at the concentration.Conclusion:Thus, some essential oils used as anti-inflammatory remedies suppress neutrophil activation by TNF-α at a low concentration (0.0125-0.025%)in vitro.

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Modulatory effect of Syzygium aromaticum and Pelargonium graveolens on oxidative stress and inflammation

10.1101/362426 ◽

2018 ◽

Author(s):

Ilias Marmouzi ◽

El Mostafa Karym ◽

Rachid Alami ◽

Meryem El Jemli ◽

Mourad Kharbach ◽

...

Keyword(s):

Oxidative Stress ◽

Essential Oils ◽

Toxic Effect ◽

Tetrahymena Pyriformis ◽

Syzygium Aromaticum ◽

Minimal Risk ◽

Pelargonium Graveolens ◽

Anti Inflammatory

AbstractBackgroundTherapy combination is defined as disease treatment with two or more medication to acheive efficacy with lower doses or lower toxicity. Regarding its reported toxicities and efficacy, the Essential Oils (EOs) from Syzygium aromaticum (SA) and Pelargonium graveolens (PG) were combined for in vitro and in vivo assays and toxicities.MethodsThe Essential Oils and mixture were tested for in vivo/in vitro antioxidant and anti-inflammatory activities. The assays included the animal model of acute inflammation (carrageenan model), the protective effect on H2O2/Sodium nitroprissude induced stress in Tetrahymena pyriformis, and the in vitro antioxidant assays.ResultsThe chemical analysis of the investigated Oils has lead to the identification of Eugenol (74.06%), Caryophyllene (11.52%) and Carvacrol acetate (7.82%) as the major element in SA; while PG was much higher in Citronellol (30.77%), 10-epi-γ-Eudesmol (22.59%), and Geraniol (13.95%). In our pharmacological screening of samples, both Oils demonstrated good antioxidant effects. In vivo investigation of the antioxidant activity in the protozoa model (T. pyriformis) demonstrated a lesser toxic effect of EOs mixture with no significant differences when oxidative stress markers and antioxidant enzymes (MDA, SOD and CAT) were evaluated. On the other hand the in vivo model of inflammatory response to carrageenan demonstrated a good inhibitory potential of both EOs. The EOs Mixture demonstrated equivalent bioactivity with lower toxic effect and minimal risk for each compound.ConclusionsThe results from this study indicate that EOs mixture from SA and PG demonstrated promising modulatory antioxidant/anti-inflammatory effect, which suggest an efficient association for therapy.

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Phytochemical screening and in vitro antibacterial activity of Moringa oleifera (Lam.) leaf extract

Archives of Agriculture and Environmental Science ◽

10.26832/24566632.2018.030406 ◽

2018 ◽

Vol 3 (4) ◽

pp. 367-372

Author(s):

S.P.K. Malhotra ◽

T.K. Mandal

Keyword(s):

Antibacterial Activity ◽

Leaf Extract ◽

Moringa Oleifera ◽

Phytochemical Screening ◽

In Vitro Antibacterial Activity ◽

Moringa Oleifera Lam

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Trichomes Morphology and Essential Oils Characterization of Field-Growing and In Vitro Propagated Plants of Lavandula pedunculata

Microscopy and Microanalysis ◽

10.1017/s143192760808971x ◽

2008 ◽

Vol 14 (S3) ◽

pp. 148-149 ◽

Cited By ~ 4

Author(s):

M. Zuzarte ◽

A.M. Dinis ◽

C. Cavaleiro ◽

J. Canhoto ◽

L. Salgueiro

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oils ◽

Vegetative Propagation ◽

Leaf Trichomes ◽

Economic Exploitation ◽

Selection Of

The selection of native Lavandula species and their economic exploitation have increased in the last few years. Micropropagation techniques have been used as an alternative for vegetative propagation allowing the multiplication of selected genotypes and chemotypes. Our previous studies showed that the essential oils of Lavandula pedunculata have an important antifungal activity against dermatophyte strains. Therefore, a new line of investigation concerning the in vitro culture of this species is justified. In the present study we compare the morphology of the leaf trichomes and the chemical composition of their essential oils in both field-growing and in vitro propagated plants.

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