scholarly journals Draft genome sequence of first Vibrio diabolicus in Mexico strain InDRE-D1-M1, an emergent threat

2021 ◽  
Vol 15 (11) ◽  
pp. 1750-1754
Author(s):  
Elena Berenice Nava-Soberanes ◽  
Edgar Ruben Mendieta-Condado ◽  
Fabiola Garcés-Ayala ◽  
Adnan Araiza-Rodríguez ◽  
Pablo Aguilera-Perez ◽  
...  
Keyword(s):  
Complete Genome ◽  
Draft Genome ◽  
Molecular Techniques ◽  
Closely Related Species ◽  
Environmental Bacterium ◽  
Raw Shrimp ◽  
The City ◽  
Marine Environmental ◽  
Total Coverage ◽  
Vibrio Spp

Introduction: The complete genome of the marine environmental bacterium Vibrio diabolicus isolated from raw shrimp in the city of Guadalajara in the state of Jalisco in Mexico is reported here. Methodology: Vibrio spp. it was isolated and identified using standard microbiological and molecular techniques. Whole genome sequencing was performed using the Miseq system (Illumina, USA). Results: The Multi Locus Sequence Typing profile of the isolated Vibrio bacteria coincided only with 4 specific loci (atpA, gyrB, pyrH and recA) and with a total coverage of the species belonging to Vibrio spp. Analysis of the complete genome of the Vibrio isolate and other closely related species, using the genomic fingerprints of the Virtual Analysis Method for PHylogenomic fingerprint estimation (VAMPHyRe) software, revealed the clustering of this species among the clade Vibrio diabolicus. The antibiogram revealed that this strain of Vibrio diabolicus is resistant to ampicillin, which is consistent with the bioinformatic finding of the β-lactamase enzyme that hydrolyzes carbenicillin class A. Conclusions: This study demonstrated that the environmental marine bacterium Vibrio diabolicus contains carrier genes associated with pathogenicity and ecological function, which could represent a threat to public health.

scholarly journals Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

2020 ◽  
Vol 8 (6) ◽  
pp. 895 ◽  
Author(s):  
Saïd Oulghazi ◽  
Mohieddine Moumni ◽  
Slimane Khayi ◽  
Kévin Robic ◽  
Sohaib Sarfraz ◽  
...  
Keyword(s):  
Species Diversity ◽  
Complete Genome ◽  
Draft Genome ◽  
Epidemiological Studies ◽  
Gene Marker ◽  
Oxford Nanopore ◽  
Causative Agents ◽  
Genome Analyses ◽  
Oxford Nanopore Technologies

Dickeya and Pectobacterium pathogens are causative agents of several diseases that affect many crops worldwide. This work investigated the species diversity of these pathogens in Morocco, where Dickeya pathogens have only been isolated from potato fields recently. To this end, samplings were conducted in three major potato growing areas over a three-year period (2015–2017). Pathogens were characterized by sequence determination of both the gapA gene marker and genomes using Illumina and Oxford Nanopore technologies. We isolated 119 pathogens belonging to P. versatile (19%), P. carotovorum (3%), P. polaris (5%), P. brasiliense (56%) and D. dianthicola (17%). Their taxonomic assignation was confirmed by draft genome analyses of 10 representative strains of the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was observed. In tuber rotting assays, D. dianthicola isolates were more aggressive than Pectobacterium isolates. The complete genome sequence of D. dianthicola LAR.16.03.LID was obtained and compared with other D. dianthicola genomes from public databases. Overall, this study highlighted the ecological context from which some Dickeya and Pectobacterium species emerged in Morocco, and reported the first complete genome of a D. dianthicola strain isolated in Morocco that will be suitable for further epidemiological studies.

scholarly journals Whole-Genome Sequences of Two Closely Related Bacteria, Actinomyces sp. Strain Chiba101 and Actinomyces denticolens DSM 20671T

2017 ◽  
Vol 5 (14) ◽  
Author(s):  
Yu Kanesaki ◽  
Taichiro Ishige ◽  
Yuriko Sekigawa ◽  
Tomoko Kobayashi ◽  
Yasushi Torii ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Whole Genome ◽  
Genome Sequences ◽  
High Quality ◽  
Content Type

ABSTRACT Actinomyces sp. strain Chiba101, isolated from an arthritic leg joint of a pig raised in Japan, is a bacterium closely related to Actinomyces denticolens. Here, we deciphered the complete genome sequence of Actinomyces sp. Chiba101 and the high-quality draft genome sequence of A. denticolens DSM 20671T.

scholarly journals Draft Genome Sequence of “Candidatus Spirobacillus cienkowskii,” a Pathogen of Freshwater Daphnia Species, Reconstructed from Hemolymph Metagenomic Reads

2018 ◽  
Vol 7 (22) ◽  
Author(s):  
Luana Bresciani ◽  
Leandro N. Lemos ◽  
Nina Wale ◽  
Jonathan Y. Lin ◽  
Alexander T. Strauss ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Draft Genome ◽  
Gc Content ◽  
Bacterial Pathogen ◽  
Bacterial Motility ◽  
Draft Genome Sequence ◽  
Daphnia Species ◽  
Red Color

We report here the near-complete genome sequence of “Candidatus Spirobacillus cienkowskii,” a spiral-shaped, red-pigmented uncultivated bacterial pathogen of Daphnia spp. The genome is 2.74 Mbp in size, has a GC content of 32.1%, and contains genes associated with bacterial motility and the production of carotenoids, which could explain the distinctive red color of hosts infected with this pathogen.

scholarly journals Major Release of 161 Whole-Genome Sequences from the International Pseudomonas Consortium Database

2019 ◽  
Vol 8 (13) ◽  
Author(s):  
Julie Jeukens ◽  
Jean-Guillaume Emond-Rheault ◽  
Luca Freschi ◽  
Irena Kukavica-Ibrulj ◽  
Roger C. Levesque
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Draft Genome ◽  
Opportunistic Pathogen ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Environmental Bacterium

Pseudomonas aeruginosa is an environmental bacterium and opportunistic pathogen. Here, we present draft genome sequences for 161 isolates from diverse clinical and environmental sources.

scholarly journals Draft Genome of the Macadamia Husk Spot Pathogen, Pseudocercospora macadamiae

2020 ◽  
Vol 110 (9) ◽  
pp. 1503-1506
Author(s):  
Olufemi A. Akinsanmi ◽  
Lilia C. Carvalhais
Keyword(s):  
Plant Disease Resistance ◽  
Plant Disease ◽  
De Novo ◽  
Draft Genome ◽  
Gc Content ◽  
Disease Development ◽  
Closely Related Species ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
The Family

Pseudocercospora macadamiae causes husk spot in macadamia in Australia. Lack of genomic resources for this pathogen has restricted acquiring knowledge on the mechanism of disease development, spread, and its role in fruit abscission. To address this gap, we sequenced the genome of P. macadamiae. The sequence was de novo assembled into a draft genome of 40 Mb, which is comparable to closely related species in the family Mycosphaerellaceae. The draft genome comprises 212 scaffolds, of which 99 scaffolds are over 50 kb. The genome has a 49% GC content and is predicted to contain 15,430 protein-coding genes. This draft genome sequence is the first for P. macadamiae and represents a valuable resource for understanding genome evolution and plant disease resistance.

Occurrence and Characterization of Escherichia coli O157 and Other Serotypes in Raw Meat Products in Morocco

2008 ◽  
Vol 71 (10) ◽  
pp. 2082-2086 ◽  
Author(s):  
LUCIANO BENEDUCE ◽  
GIUSEPPE SPANO ◽  
ARI Q. NABI ◽  
FRANCESCO LAMACCHIA ◽  
SALVATORE MASSA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Susceptibility Testing ◽  
Meat Products ◽  
Molecular Techniques ◽  
Escherichia Coli O157 ◽  
Raw Meat ◽  
E Coli ◽  
Meat Samples ◽  
The City

In this study, 100 raw meat samples were collected from 15 local Moroccan butcheries in five different areas of the city of Rabat during a period of 4 months. Overall, 7 of 15 butcheries from three areas of the city yielded strains of Escherichia coli O157. Single isolates from 9 (9%) of 100 raw meat samples were biochemically and serologically confirmed as E. coli O157. Using molecular techniques, two strains were positive for the Shiga toxin, with two additional strains containing an attaching-effacing gene. All potentially virulent serotypes isolated from these meat samples showed distinct pulsed-field gel electrophoresis profiles. Based on antibiotic susceptibility testing, more than 70% of the isolates were resistant to ampicillin and clavulanic acid–amoxicillin. Moreover, one strain was resistant to more than three antibiotics. Our study represents the first survey of E. coli O157 and related serotypes in raw meat products in Morocco.

scholarly journals Draft Genome Sequence of Rhodococcus ruber Strain P25, an Active Polychlorinated Biphenyl Degrader

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Ekaterina S. Shumkova ◽  
Björn E. Olsson ◽  
Anna V. Kudryavtseva ◽  
Elena G. Plotnikova
Keyword(s):  
Polychlorinated Biphenyls ◽  
Genome Sequence ◽  
Aromatic Compounds ◽  
Polychlorinated Biphenyl ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Rhodococcus Ruber ◽  
The City ◽  
Halogenated Aromatic Compounds

We report the 5,728,255-bp draft genome sequence of Rhodococcus ruber P25, isolated from a soil polluted with halogenated aromatic compounds in the city of Perm, Russia. The strain degrades polychlorinated biphenyls and a broad range of aromatic compounds. It possesses genes that mediate the degradation of biphenyls/polychlorinated biphenyls, naphthalene, and monoaromatic compounds.

Comparison of the Microbial Quality of Raw Shrimp from China, Ecuador, or Mexico at Both Wholesale and Retail Levels

1994 ◽  
Vol 57 (2) ◽  
pp. 150-153 ◽  
Author(s):  
THOMAS M. BERRY ◽  
DOUGLAS L. PARK ◽  
DONALD V. LIGHTNER
Keyword(s):  
Microbial Quality ◽  
Resistance Patterns ◽  
Plate Counts ◽  
Contamination Levels ◽  
Frozen Shrimp ◽  
Source Countries ◽  
Antibiotic Resistance Patterns ◽  
Raw Shrimp ◽  
Vibrio Spp

Aerobic plate counts (APC), Listeria spp., and Vibrio spp. and antibiotic resistance patterns of Vibrio spp. were determined for imported shrimp from China, Ecuador, and Mexico obtained from wholesale/frozen and retail/previously frozen markets. Statistically significant differences in APC were observed among source countries and wholesale/frozen versus retail/previously frozen samples. Wholesale/frozen shrimp products were consistently excellent quality with respect to APC; higher contamination levels were observed in retail/previously frozen samples. Listeria spp. and L. monocytogenes were isolated from 16.7 and 6.7% of shrimp samples, respectively. Vibrio spp. were present in 63.3% of the samples, more often isolated from shrimp from Mexico or China than Ecuador. The majority of isolates were identified as V. parahaemolyticus (36.7%), V. alginolyticus (26.7%), or V. vulniftcus (16.7%), and 53.7% were resistant to at least one antibiotic. These data reveal that important differences in microbial quality occur in raw shrimp products as a function of source and between retail and Wholesale products.

scholarly journals Complete Genome Sequence of Campylobacter concisus ATCC 33237T and Draft Genome Sequences for an Additional Eight Well-Characterized C. concisus Strains

2017 ◽  
Vol 5 (29) ◽  
Author(s):  
Angela J. Cornelius ◽  
William G. Miller ◽  
Albert J. Lastovica ◽  
Stephen L. W. On ◽  
Nigel P. French ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Draft Genome ◽  
Strain Atcc ◽  
Genome Sequences ◽  
Campylobacter Concisus ◽  
Content Type

ABSTRACT We report the complete genome sequence of the Campylobacter concisus type strain ATCC 33237 and the draft genome sequences of eight additional well-characterized C. concisus strains. C. concisus has been shown to be a genetically heterogeneous species, and these nine genomes provide valuable information regarding the diversity within this taxon.

scholarly journals Complete Genome Sequence of a Shiga Toxin-Producing Escherichia coli O26:H11 Strain (Sequence Type 21) and Two Draft Genome Sequences of Listeria monocytogenes Strains (Clonal Complex 1 [CC1] and CC59) Isolated from Fresh Produce in Germany

2020 ◽  
Vol 9 (49) ◽  
Author(s):  
Gregor Fiedler ◽  
Jan Kabisch ◽  
Erik Brinks ◽  
Sabrina Sprotte ◽  
Christina Boehnlein ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Shiga Toxin ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Clonal Complex ◽  
Draft Genome ◽  
Sequence Type ◽  
Genome Sequences ◽  
Content Type

ABSTRACT The complete genome sequence of a Shiga toxin-producing Escherichia coli (STEC) O26:H11 strain, MBT-5 (sequence type 21 [ST21], stx1a, stx2a, eae, ehxA), and two draft genome sequences of Listeria monocytogenes strains MBT-6 and MBT-7 belonging to the virulent sequence types 1 (ST1, clonal complex 1 [CC1]) and 59 (ST59, CC59), respectively, were determined. The strains were isolated in 2015 from ready-to-eat mixed greens in Germany.

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