scholarly journals The Effect of Brown Rice on Superoxide Dismutase Level and Non-alcoholic Fatty Liver in an Sprague–Dawley Rat Model of High-fat High-fructose Diet-induced Obesity

2020 ◽  
Vol 8 (A) ◽  
pp. 873-880
Author(s):  
Inggita Kusumastuty ◽  
Dian Handayani ◽  
Shafira Hanifa ◽  
Melinda Lisan ◽  
Etik Sulistyowati
Keyword(s):  
Superoxide Dismutase ◽  
Fatty Liver ◽  
Rat Model ◽  
Brown Rice ◽  
Diet Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rat ◽  
High Fructose

BACKGROUND: Obesity can cause oxidative stress due to the release of free radical components or reactive oxygen species. The accumulation of excess fat in obesity also causes the occurrence of non-alcoholic fatty liver. Brown rice is a functional food with higher fiber, vitamin, mineral, and antioxidant contents than those in white rice. AIM: This study was aimed to evaluate the effect of brown rice on the superoxide dismutase (SOD) level and non-alcoholic fatty liver in obese Sprague–Dawley rats. METHODS: An experimental study using a post-test-only control group design was carried out on a Sprague–Dawley rat model of obesity-induced by a high-fat high fructose (HFHF) diet. Sample selection was done through simple random sampling; rats were divided into five groups, namely the normal diet group (K−), the HFHF diet group (K+), and HFHF diet groups with the addition of brown rice dose I (P1), dose II (P2), and dose III (P3). The measured variable was the SOD level measured using the spectrophotometric method and the calculation of the percentage of fatty liver cells on the results of a hematoxylin-eosin liver scan of a rat’s right lobe. RESULTS: The dose of brown rice was not affected body weight of the rats significantly compared to the control groups. Then, there were significant differences in the SOD level and fatty liver in at least two groups (p = 0.01 and p = 0.001). CONCLUSION: This study concludes that the administration of brown rice contributes to the increasing SOD level and decreasing fatty liver in obese model rats.

scholarly journals Alleviating effect of Ficus racemosa in high-fat-high-fructose diet-induced non-alcoholic fatty liver disease

2021 ◽  
Vol 65 ◽  
pp. 12-20
Author(s):  
Nilay D. Solanki ◽  
Kirti Vadi ◽  
Sandip Patel
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Lipid Profile ◽  
Fatty Liver Disease ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
Serum Leptin ◽  
High Fructose ◽  
Ficus Racemosa ◽  
Alcoholic Fatty Liver Disease

Objectives: Non-alcoholic fatty liver disease (NAFLD) is one of the chronic liver diseases. Ficus racemosa has been used for many years in the Ayurvedic medicine system and is closely related with the management of metabolic conditions. The study investigated alleviating effects of methanolic extract of F. racemosa (FRM) bark on high-fat-high-fructose (HFHF) diet -induced NAFLD. Materials and Methods: HFHF-based model was developed for a period of 10 weeks. In treatment groups, FRM (100 mg/kg, 200 mg/kg, and 400 mg/kg) and atorvastatin (20 mg/kg) were administered for 6 weeks after initiating HFHF diet and continued for another 4 weeks. Liver functions test, lipid profile, serum leptin, and antioxidant parameters and histopathology were evaluated. Results: Elevated liver enzymes, lipid markers, and leptin were observed, with significant reduction in antioxidants in disease control rats. FRM treatment significantly improved serum aminotransferase activities, lipid profile, and oxidative changes and brought back to normal. Liver histopathology showed the fatty modifications induced by the HFHF diet, and reduction in fatty changes was observed due to FRM. Significant decline in serum leptin was observed with high-dose FRM. Conclusion: FRM showed positive effects in the reversal of NAFLD and different polyphenolic compounds in the plant were responsible for the proven action.

scholarly journals Antrodan Alleviates High-Fat and High-Fructose Diet-Induced Fatty Liver Disease in C57BL/6 Mice Model via AMPK/Sirt1/SREBP-1c/PPARγ Pathway

2020 ◽  
Vol 21 (1) ◽  
pp. 360 ◽  
Author(s):  
Charng-Cherng Chyau ◽  
Hsueh-Fang Wang ◽  
Wen-Juan Zhang ◽  
Chin-Chu Chen ◽  
Shiau-Huei Huang ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Mice Model ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
High Fructose Diet ◽  
High Fructose ◽  
Ant Control ◽  
Alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) and -steatohepatitis (NASH) imply a state of excessive fat built-up in livers with/or without inflammation and have led to serious medical concerns in recent years. Antrodan (Ant), a purified β-glucan from A. cinnamomea has been shown to exhibit tremendous bioactivity, including hepatoprotective, antihyperlipidemic, antiliver cancer, and anti-inflammatory effects. Considering the already well-known alleviating bioactivity of A. cinnamomea for the alcoholic steatohepatitis (ASH), we propose that Ant can be beneficial to NAFLD, and that the AMPK/Sirt1/PPARγ/SREBP-1c pathways may be involved in such alleviations. To uncover this, we carried out this study with 60 male C57BL/6 mice fed high-fat high-fructose diet (HFD) for 60 days, in order to induce NAFLD/NASH. Mice were then grouped and treated (by oral administration) as: G1: control; G2: HFD (HFD control); G3: Ant, 40 mgkg (Ant control); G4: HFD+Orlistat (10 mg/kg) (as Orlistat control); G5: HFD+Ant L (20 mg/kg); and G6: HFD+Ant H (40 mg/kg) for 45 days. The results indicated Ant at 40 mg/kg effectively suppressed the plasma levels of malondialdehyde, total cholesterol, triglycerides, GOT, GPT, uric acid, glucose, and insulin; upregulated leptin, adiponectin, pAMPK, Sirt1, and down-regulated PPARγ and SREBP-1c. Conclusively, Ant effectively alleviates NAFLD via AMPK/Sirt1/CREBP-1c/PPARγ pathway.

scholarly journals Cynanchum atratum Alleviates Non-Alcoholic Fatty Liver by Balancing Lipogenesis and Fatty Acid Oxidation in a High-Fat, High-Fructose Diet Mice Model

Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 23
Author(s):  
Jing-Hua Wang ◽  
Seung-Ju Hwang ◽  
Dong-Woo Lim ◽  
Chang-Gue Son
Keyword(s):  
Fatty Acid ◽  
Fatty Liver ◽  
Fatty Acid Oxidation ◽  
The Body ◽  
Acid Oxidation ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
High Fructose Diet ◽  
High Fructose ◽  
Tnf Α

Cynanchum atratum, a medicinal herb, is traditionally used as an antidote, diuretic, and antipyretic in eastern Asia. The current study aimed to investigate the anti-fatty liver capacity of the ethanol extract of Cynanchum atratum (CAE) using a 10-week high-fat, high-fructose diet mouse model. A six-week treatment of CAE (from the fifth week) significantly attenuated the weights of the body, liver, and mesenteric fat without a change in diet intake. CAE also considerably restored the alterations of serum aminotransferases and free fatty acid, fasting blood glucose, serum and hepatic triglyceride, and total cholesterol, as well as platelet and leukocyte counts. Meanwhile, CAE ameliorated hepatic injury and lipid accumulation, as evidenced by histopathological and immunofluorescence observations. Additionally, CAE significantly lowered the elevation of hepatic TNF-α, the TNF-α/IL-10 ratio, fecal endotoxins, and the abundance of Gram-negative bacteria. Hepatic lipogenesis and β-oxidation-related proteins and gene expression, including PPAR-α, SREBP-1, SIRT1, FAS, CTP1, etc., were normalized markedly by CAE. In particular, the AMPK, a central regulator of energy metabolism, was phosphorylated by CAE at an even higher rate than metformin. Overall, CAE exerts anti-hepatic steatosis effects by reducing lipogenesis and enhancing fatty acid oxidation. Consequently, Cynanchum atratum is expected to be a promising candidate for treating chronic metabolic diseases.

scholarly journals High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat

Adipocyte ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Cláudia Marques ◽  
Manuela Meireles ◽  
Sónia Norberto ◽  
Joana Leite ◽  
Joana Freitas ◽  
...  
Keyword(s):  
Rat Model ◽  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Sprague Dawley Rat

scholarly journals High-Fructose, High-Fat Diet Alters Muscle Composition and Fuel Utilization in a Juvenile Iberian Pig Model of Non-Alcoholic Fatty Liver Disease

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4195
Author(s):  
Heather C. Spooner ◽  
Stefani A. Derrick ◽  
Magdalena Maj ◽  
Rodrigo Manjarín ◽  
Gabriella V. Hernandez ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Liver Disease ◽  
Fatty Liver ◽  
High Fat Diet ◽  
Fatty Liver Disease ◽  
Fuel Utilization ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
High Fructose ◽  
Alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) is a serious metabolic condition affecting millions of people worldwide. A “Western-style diet” has been shown to induce pediatric NAFLD with the potential disruption of skeletal muscle composition and metabolism. To determine the in vivo effect of a “Western-style diet” on pediatric skeletal muscle fiber type and fuel utilization, 28 juvenile Iberian pigs were fed either a control diet (CON) or a high-fructose, high-fat diet (HFF), with or without probiotic supplementation, for 10 weeks. The HFF diets increased the total triacylglycerol content of muscle tissue but decreased intramyocellular lipid (IMCL) content and the number of type I (slow oxidative) muscle fibers. HFF diets induced autophagy as assessed by LC3I and LC3II, and inflammation, as assessed by IL-1α. No differences in body composition were observed, and there was no change in insulin sensitivity, but HFF diets increased several plasma acylcarnitines and decreased expression of lipid oxidation regulators PGC1α and CPT1, suggesting disruption of skeletal muscle metabolism. Our results show that an HFF diet fed to juvenile Iberian pigs produces a less oxidative skeletal muscle phenotype, similar to a detraining effect, and reduces the capacity to use lipid as fuel, even in the absence of insulin resistance and obesity.

scholarly journals Effects of Dietary Brown Rice on Carcass Composition and Nitric Oxide (NOx) Metabolite Levels in High-Fat High-Fructose Diet-induced Sprague Dawley Rats as Obesity Model

2020 ◽  
Vol 1665 ◽  
pp. 012007
Author(s):  
Dian Handayani ◽  
Ahmad Ramadhan ◽  
Risma Debby Anindyanti ◽  
Alma Maghfirotun Innayah ◽  
Etik Sulistyowati ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Brown Rice ◽  
Carcass Composition ◽  
Sprague Dawley ◽  
High Fat ◽  
High Fructose Diet ◽  
Sprague Dawley Rats ◽  
High Fructose

scholarly journals Improvement of Hypertriglyceridemia by Roasted Nelumbinis folium in High Fat/High Cholesterol Diet Rat Model

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3859
Author(s):  
Hye Yoom Kim ◽  
Mi Hyeon Hong ◽  
Kwan Woo Kim ◽  
Jung Joo Yoon ◽  
Jung Eun Lee ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Rat Model ◽  
Vascular Function ◽  
Inhibitory Effect ◽  
Diet Group ◽  
Epididymal Adipose Tissue ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Sprague Dawley ◽  
High Fat

Hypertriglyceridemia is a condition characterized by high triglyceride levels and is a major risk factor for the development of cardiovascular diseases. The present study was designed to investigate the inhibitory effect of roasted Nelumbinis folium (RN), which is a medicinal substance produced by heating lotus leaves, on lipid metabolism in high fat/cholesterol (HFC) diet-induced hypertriglyceridemia. Except for those in the control group, Sprague–Dawley rats were fed an HFC diet for four weeks to induce hypertriglyceridemia. During the next nine weeks, the control, regular diet; HFC, HFC diet, FLU, fluvastatin (3 mg/kg/day); RNL, RN (100 mg/kg/day); RNH, RN (200 mg/kg/day) were orally administered together with the diet, and the experiments were conducted for a total of 13 weeks. The weight of the epididymal adipose tissue, liver, and heart of rats in the HFC diet group significantly increased compared to those in the control group but improved in the RN-treated group. It was also confirmed that vascular function, which is damaged by an HFC diet, was improved after RN treatment. The levels of insulin, glucose, triglycerides, total cholesterol, and low-density lipoprotein increased in the HFC diet group compared to those in the control group, while the administration of RN attenuated these parameters. In addition, the administration of RN significantly reduced the gene expression of both LXR and SREBP-1, which indicated the inhibitory effect of the biosynthesis of triglycerides caused by RN. The results indicated that RN administration resulted in an improvement in the overall lipid metabolism and a decrease in the concentration of triglycerides in the HFC diet-induced rat model of hypertriglyceridemia. Therefore, our findings suggest that the RN can be a candidate material to provide a new direction for treating hypertriglyceridemia.

scholarly journals Beneficial Effects of Lactobacillus plantarum Strains on Non-Alcoholic Fatty Liver Disease in High Fat/High Fructose Diet-Fed Rats

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 542 ◽  
Author(s):  
Eun-Jung Park ◽  
You-Suk Lee ◽  
Sung Min Kim ◽  
Gun-Seok Park ◽  
Yong Hyun Lee ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Lactobacillus Plantarum ◽  
Fatty Liver Disease ◽  
Fecal Microbiota ◽  
Antioxidant Enzyme Activities ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
High Fructose ◽  
Alcoholic Fatty Liver Disease

Emerging evidence suggests that probiotics are beneficial in non-alcoholic fatty liver disease (NAFLD). This study aimed to explore the effects of two Lactobacillus plantarum strains, ATG-K2 and ATG-K6 (isolated from Korean fermented cabbage), in a rat model of high fat/high fructose (HF/HF) diet-induced NAFLD. Rats with NAFLD were randomized into four groups (HF/HF diet control, (HC); HF/HF diet with silymarin, (PC); HF/HF diet with ATG-K2, (K2); and HF/HF diet with ATG-K6, (K6)) with healthy rats on a normal diet serving as the negative control. After treatment, histopathological and biochemical analyses of the blood and liver tissue were conducted. In addition, fecal microbiota was analyzed using the MiSeq platform. Compared with HC rats, K2 and K6 rats experienced significantly lower body weight gain, displayed decreased hepatic lipid accumulation, had lower serum levels of aspartate aminotransferase and alanine aminotransferase, and showed increased antioxidant enzyme activities. Moreover, de novo lipogenesis-related genes were downregulated following K2 and K6 administration. The fecal microbiota of K2 and K6 rats contained a higher proportion of Bacteriodetes and a lower proportion of Fimicutes than that of HC rats. Taken together, our results suggest that L. plantarum strains ATG-K2 and ATG-K6 are potential therapeutic agents for NAFLD.

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