Relationship between CD 163 Tumor-Associated Macrophages and Colorectal-Cancer Stem Cell Markers

BACKGROUND: Colorectal-cancer stem cells (CR-CSCs) represent a specific subpopulation of colorectal cancer (CRC) cells, which are characterized by the expression of CD133 and CD166. Tumor-associated macrophages (TAMs), found near CSCs may represent polarized macrophages, which are characterized by CD163 expression. In most tumors, TAMs may promote aggressive tumor development, leading to poor prognoses. AIM: The aim of this study was to determine whether any association exists between CD163 expression in TAMs and CD133 and CD166 expression in CR-CSCs. METHODS: This study used a cross-sectional design that was conducted at the General Hospital and affiliates in Medan, from September 2018 to July 2019. CRC tissues were collected from colonoscopy biopsies and surgical resections performed on CRC patients, who fulfilled all necessary inclusion and exclusion criteria and provided informed consent. Subjects were divided into high- and low-CD163-level groups. We analyzed the expression levels of CD163, CD133, and CD166 using immunohistochemical (IHC) assays. RESULTS: A total of 118 CRC patients were enrolled in this study, of whom 58.5% were male. No significant differences in hemoglobin, leukocyte, or platelet levels were observed between high- and low-level CD163 expression. We didn’t find any significant association of CD163 TAM with CRC histological grade and TNM stagings. Significant associations were found between the CD 163 expression level and the CD133 expression level (p < 0.001) and between the CD 163 expression level and the CD166 expression level (p< 0.001). Increased TAM levels of CD163 was associated with 2.770-fold and 2.616-fold increased risks of elevated CD133 and CD166 levels, respectively. CONCLUSION: An association was found between the expression levels of CD163 in TAMs and the expression levels of CD133 and CD166 in CR-CSCs.

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ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro

10.31083/jomh.2021.072 ◽

2021 ◽

Keyword(s):

Colorectal Cancer ◽

Stem Cells ◽

Tumor Development ◽

Stem Cell Markers ◽

Activating Transcription Factor 3 ◽

Cancer Stem Cell Markers ◽

Cellular Context ◽

Activating Transcription Factor ◽

Sw620 Cells

Background and objective: Activating transcription factor 3 (ATF3) plays a crucial role in regulating tumor development depending on the cellular context or cancer cell type. However, the effect of ATF3 on stem cells-like features in colorectal cancer (CRC) has yet to be elaborated. Methods: In this study, we overexpressed ATF3 in SW620 CRC cells to investigate its effects on stem cells-like features. Results: Our results indicated that overexpressing ATF3 inhibited the proliferation, invasion, migration, and sphere formation capacity of SW620 CRC cells. ATF3 overexpression also decreased the size of tumorspheres and reduced expression of the cancer stem cell markers CD44 and CD133 in SW620 cells. Conclusion: In summary, our study revealed that ATF3 suppresses CRC growth and stem cells-like features. ATF3 is considered a potential target in CRC therapy.

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Faculty Opinions recommendation of ILK Expression in Colorectal Cancer Is Associated with EMT, Cancer Stem Cell Markers and Chemoresistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733254991.793550602 ◽

2018 ◽

Author(s):

Avri Ben-Ze'ev

Keyword(s):

Colorectal Cancer ◽

Stem Cell ◽

Cancer Stem Cell ◽

Stem Cell Markers ◽

Cell Markers ◽

Cancer Stem Cell Markers

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N-Glycomic and Transcriptomic Changes Associated with CDX1 mRNA Expression in Colorectal Cancer Cell Lines

Cells ◽

10.3390/cells8030273 ◽

2019 ◽

Vol 8 (3) ◽

pp. 273 ◽

Cited By ~ 5

Author(s):

Stephanie Holst ◽

Jennifer Wilding ◽

Kamila Koprowska ◽

Yoann Rombouts ◽

Manfred Wuhrer

Keyword(s):

Colorectal Cancer ◽

Mrna Expression ◽

Cell Lines ◽

Cancer Cell ◽

Tumor Development ◽

Cancer Cell Lines ◽

Expression Levels ◽

Nuclear Factors ◽

Homeobox Protein ◽

Regulated Gene Expression

The caudal-related homeobox protein 1 (CDX1) is a transcription factor, which is important in the development, differentiation, and homeostasis of the gut. Although the involvement of CDX genes in the regulation of the expression levels of a few glycosyltransferases has been shown, associations between glycosylation phenotypes and CDX1 mRNA expression have hitherto not been well studied. Triggered by our previous study, we here characterized the N-glycomic phenotype of 16 colon cancer cell lines, selected for their differential CDX1 mRNA expression levels. We found that high CDX1 mRNA expression associated with a higher degree of multi-fucosylation on N-glycans, which is in line with our previous results and was supported by up-regulated gene expression of fucosyltransferases involved in antenna fucosylation. Interestingly, hepatocyte nuclear factors (HNF)4A and HNF1A were, among others, positively associated with high CDX1 mRNA expression and have been previously proven to regulate antenna fucosylation. Besides fucosylation, we found that high CDX1 mRNA expression in cancer cell lines also associated with low levels of sialylation and galactosylation and high levels of bisection on N-glycans. Altogether, our data highlight a possible role of CDX1 in altering the N-glycosylation of colorectal cancer cells, which is a hallmark of tumor development.

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Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM, and ALDH1 in colorectal cancer

British Journal of Cancer ◽

10.1038/sj.bjc.6605762 ◽

2010 ◽

Vol 103 (3) ◽

pp. 382-390 ◽

Cited By ~ 167

Author(s):

A Lugli ◽

G Iezzi ◽

I Hostettler ◽

M G Muraro ◽

V Mele ◽

...

Keyword(s):

Colorectal Cancer ◽

Stem Cell ◽

Cancer Stem Cell ◽

Prognostic Impact ◽

Stem Cell Markers ◽

Cell Markers ◽

Cancer Stem Cell Markers

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Rationales for the Use of Cancer Stem Cells Markers in the Staging of Papillary Thyroid Carcinoma

Journal of Oncology ◽

10.1155/2019/1659654 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Noah Abd-Alkader Mahmood ◽

Amer Talib Tawfeeq ◽

Israa Mhdi Al-Sudani ◽

Zaynab Saad Abd-Alghni

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Histopathological Examination ◽

Standard Procedure ◽

Tumor Stage ◽

Needle Aspiration ◽

Stem Cell Markers ◽

Papillary Thyroid ◽

Expression Levels ◽

Cancer Stem Cell Markers

Fine needle aspiration biopsy (FNAB) is a standard procedure for the detection of thyroid nodules malignancy, yet 10-25% of the sample diagnosed may go undetermined or suspicious. The utility of cancer stem cell markers (CSCM) as a differential diagnosis molecular marker in nodules of suspicious decision in FNAB was hypothesized. Papillary thyroid carcinoma (PTC) and thyroid fibroadenoma (TFA) samples were selected to test the hypothesis. The samples employed in this study were from patients who had thyroid hyperplasia and a suspicious or undetermined diagnosis by FNAB. The patient underwent a successful thyroidectomy at Al-Yarmouk Teaching Hospital in Baghdad between January 2015 and December 2017. All nodule samples underwent a systematic histopathological examination after resection. Tumors diagnosed as PTC and those diagnosed as fibroadenoma (TFA) were selected for this study. Collectively 39 PTC and 11 TFA nodules were included. Quantitative reverse transcriptase real-time PCR (qRT-PCR) and immunohistochemistry (IHC) were used to determine levels of mRNA and proteins of CSCM ALDH1A1, CD44, ABCG2, and Oct3/4 in both types of tumors were used. This study revealed that the expression levels of CSCM were significantly increased in PTC tissues when compared to benign tissues and the positive correlation was found between the CSCM expression levels and tumor stage, size, and gender. In conclusion, for a more precise diagnosis, we suggest these markers be included in what is currently available to characterize malignancy from what is not in thyroid cancer, as well as for the staging process of PTC.

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Colon cancer stem cell markers CD44 and CD133 in patients with colorectal cancer and synchronous hepatic metastases

International Journal of Oncology ◽

10.3892/ijo.2015.2844 ◽

2015 ◽

Vol 46 (4) ◽

pp. 1582-1588 ◽

Cited By ~ 45

Author(s):

FEIFENG JING ◽

HUN JIN KIM ◽

CHANG HYUN KIM ◽

YOUNG JIN KIM ◽

JAE HYUK LEE ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Stem Cell ◽

Cancer Stem Cell ◽

Hepatic Metastases ◽

Stem Cell Markers ◽

Cell Markers ◽

Cancer Stem Cell Markers ◽

Colon Cancer Stem Cell

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Identification of differentially expressed circular RNAs in human colorectal cancer

Tumor Biology ◽

10.1177/1010428317694546 ◽

2017 ◽

Vol 39 (3) ◽

pp. 101042831769454 ◽

Cited By ~ 31

Author(s):

Peili Zhang ◽

Zhigui Zuo ◽

Wenjing Shang ◽

Aihua Wu ◽

Ruichun Bi ◽

...

Keyword(s):

Colorectal Cancer ◽

Receiver Operating Characteristic ◽

Operating Characteristic ◽

Circular Rna ◽

Expression Level ◽

Circular Rnas ◽

Human Colorectal Cancer ◽

Expression Levels ◽

Normal Tissues ◽

Node Metastasis

Circular RNA, a class of non-coding RNA, is a new group of RNAs and is related to tumorigenesis. Circular RNAs are suggested to be ideal candidate biomarkers with potential diagnostic and therapeutic implications. However, little is known about their expression in human colorectal cancer. In our study, differentially expressed circular RNAs were detected using circular RNA array in paired tumor and adjacent non-tumorous tissues from six colorectal cancer patients. Expression levels of selected circular RNAs (hsa_circRNA_103809 and hsa_circRNA_104700) were measured by real-time polymerase chain reaction in 170 paired colorectal cancer samples for validation. Statistical analyses were conducted to investigate the association between hsa_circRNA_103809 and hsa_circRNA_104700 expression levels and respective patient clinicopathological features. Receiver operating characteristic curve was constructed to evaluate the diagnostic values. Our results indicated that there were 125 downregulated and 76 upregulated circular RNAs in colorectal cancer tissues compared with normal tissues. We also first demonstrated that the expression levels of hsa_circRNA_103809 ( p < 0.0001) and hsa_circRNA_104700 ( p = 0.0003) were significantly lower in colorectal cancer than in normal tissues. The expression level of hsa_circRNA_103809 was significantly correlated with lymph node metastasis ( p = 0.021) and tumor-node-metastasis stage ( p = 0.011), and the expression level of hsa_circRNA_104700 was significantly correlated with distal metastasis ( p = 0.036). The area under receiver operating characteristic curves of hsa_circRNA_103809 and hsa_circRNA_104700 were 0.699 ( p < 0.0001) and 0.616 ( p < 0.0001), respectively. In conclusion, these results suggest that hsa_circRNA_103809 and hsa_circRNA_104700 may be potentially involved in the development of colorectal cancer and serve as potential biomarkers for the diagnosis of colorectal cancer.

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