scholarly journals Mixture design applied to the study of bioethanol production from cheese whey and corn steep liquor

2016 ◽  
Vol 7 (3) ◽  
pp. 150 ◽  
Author(s):  
Fabiane Oliveira Farias ◽  
Aline Alberti ◽  
Alessandro Nogueira ◽  
Ivo Mottin Demiate
Keyword(s):  
Cheese Whey ◽  
Raw Materials ◽  
Corn Steep Liquor ◽  
Lactic Acid Production ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Design Tool ◽  
Fermentation Efficiency ◽  
Steep Liquor ◽  
By Products

Apart from creating many positive impacts, the growth of agribusiness has also generates concerns about the production of large amounts of undervalued by-products. Cheese whey and corn steep liquor (from corn flour production, a typical Brazilian food) are among these by-products that can be potential raw materials for obtaining new products such as bioethanol. The objective of this study was to apply an experimental design tool to examine different worts with cheese whey and corn steep liquor for the production of bioethanol. The worts containing 100 % corn steep liquor, and 25 % cheese whey with 75 % corn steep liquor supplemented with glucose were considered the best for the production of bioethanol, with fermentation efficiency reaching around 90 % for both of them. The use of pasteurization and antibiotics was effective in controlling lactic acid production, which results from bacterial contamination. The use of antibiotics showed advantages by reducing the maximum growth rate of yeast, which was reflected in higher rates of fermentation efficiency. The results obtained allowed reaching a high level of ethanol productivity. 

scholarly journals Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 365 ◽  
Author(s):  
Nathália S. A. A. Marques ◽  
Israel G. Sales da Silva ◽  
Davi L. Cavalcanti ◽  
Patrícia C. S. V. Maia ◽  
Vanessa P. Santos ◽  
...  
Keyword(s):  
Canola Oil ◽  
Raw Materials ◽  
Corn Steep Liquor ◽  
Mucor Circinelloides ◽  
Artemia Salina ◽  
Engine Oil ◽  
Extreme Conditions ◽  
Linear Quadratic ◽  
Statistical Validation ◽  
Steep Liquor

The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. In the present work, an economically attractive medium composed of corn steep liquor and waste cooking oil was formulated to maximize the production of bioemulsifier by Mucor circinelloides UCP0001. A central rotational composite design was applied to statistical validation of the production. The emulsifying properties, stability under extreme conditions, its toxicity character, and the characterization of the bioemulsifier were determined. The best condition for biomolecule synthesis occurred in the assay 2 containing 4% of corn steep liquor and 3% waste soybean oil and exhibited 100% emulsification index for canola oil and petroleum, as well as excellent emulsifying activity for canola oil and burned engine oil. The nutritional factors studied showed statistical relevance, since all linear, quadratic effects and their interactions were significant. The bioemulsifier showed 2.69 g/L yield and the chemical character of the molecule structure was identified by FT-IR (Fourier Transform Infrared) spectroscopy. The bioemulsifier showed no toxicity to Artemia salina and Chlorella vulgaris. Stable emulsions were obtained under extreme conditions of temperature, pH, and salinity. These findings contribute to understanding of the relationship between production, physical properties, chemical composition, and stability of bioemulsifier for their potential applications in biotechnology, such as bioremediation of hydrocarbon-contaminated soil and water.

Highly efficient l-lactic acid production from xylose in cell recycle continuous fermentation using Enterococcus mundtii QU 25

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17659-17668 ◽  
Author(s):  
Mohamed Ali Abdel-Rahman ◽  
Yukihiro Tashiro ◽  
Takeshi Zendo ◽  
Kenji Sakai ◽  
Kenji Sonomoto
Keyword(s):  
Lactic Acid ◽  
Continuous Fermentation ◽  
Corn Steep Liquor ◽  
Lactic Acid Production ◽  
High Concentration ◽  
Cell Recycle ◽  
Enterococcus Mundtii ◽  
Cell Recycling ◽  
Steep Liquor ◽  
Feeding Medium

We report an effective cell recycling continuous fermentation of xylose to l-lactic acid with high concentration, productivity, and yield using strain QU 25. pH was found to affect the yield and corn steep liquor as feeding medium enhanced the yield.

scholarly journals Biohydrogen Production From Agro-industrial Wastes Using Clostridium Beijerinckii and Isolated Bacteria as Inoculum

2021 ◽  
Author(s):  
Walter José Martinez-Burgos ◽  
Jair Rosário do Nascimento Junior ◽  
Adriane Bianchi Pedroni Medeiros ◽  
Leonardo Wedderhoff Herrmann ◽  
Eduardo Bittencourt Sydney ◽  
...  
Keyword(s):  
Amino Acid ◽  
Organic Acid ◽  
Cheese Whey ◽  
Soft Drink ◽  
Corn Steep Liquor ◽  
Clostridium Beijerinckii ◽  
Industrial Wastes ◽  
Biohydrogen Production ◽  
Fermentation Time ◽  
Steep Liquor

Abstract The search for renewable and sustainable sources of energy has been one of the main goals of society in recent years, especially to reduce the environmental impacts of fossil fuels. One promising alternative is the production of hydrogen, which does not emit greenhouse gases and can be produced from agro-industrial wastes. The Clostridium genus is recorded as having high hydrogen yields compared to other genus, with several producing species. The objective of this work was to evaluate biohydrogen production potential of four agro-industrial residues, which were soft drink wastewater, corn steep liquor, cheese whey, and expired Guaraná soft drink, using one model strain Clostridium beijerinckii ATCC 8260 and newly isolated Clostridium butyricum DEBB-B348. The agro-industrial wastes were characterised in terms of monosaccharide, organic acid, amino acid, cation, and anion concentrations and compared to the literature. After performing subsequent experimental designs, the significant factors were cheese whey concentration, corn steep liquor concentration, and fermentation time for C. beijerinckii, and corn steep liquor concentration and fermentation time for C. butyricum (p ≤ 0.05), with an R2 of 0.950 and 0.895, respectively. The maximum hydrogen volume production was 18.5 ± 1.68 mL and 27.4 ± 1.84 mL for each strain, respectively. The C. butyricum 16s rRNA gene phylogenetic tree and the carbohydrate, organic acid, and amino acid kinetics of the optimum medium are also presented. These results indicate a potential hydrogen production process utilising less expensive substrates, proposing more proper disposal for agro-industrial wastes and using an isolated strain with high yield.

scholarly journals PENGARUH PERTUMBUHAN DAN EFISIENSI PAKAN IKAN NILA GIFT (Oreochromis niloticus) DENGAN PROPORSI PROTEIN PAKAN YANG BERBEDA

2019 ◽  
Author(s):  
titin liana febriyanti
Keyword(s):  
Growth Rate ◽  
Oreochromis Niloticus ◽  
Feed Efficiency ◽  
Raw Materials ◽  
Economic Value ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Fish Feed ◽  
Randomized Design ◽  
High Feed

Tilapia (Oreochromis niloticus) has economic value due to high consumer demand, relatively affordable price, favorable taste and high tolerance to the environment. Constraints faced by farmers is the high feed prices because raw materials such as fish meal is still imported. So need to find alternative raw materials such as golden snail flour which has a cheap price. The purpose of this study is to analyze the proportion of the right golden snail flour in the fish feed so as to provide maximum growth rate for the seeds of tilapia gift. The experimental design used was Completely Randomized Design (RAL) consisting of 5 treatments with 3 replications. The parameters observed were growth rate and feed efficiency. The experimental results showed that the C treatment (0.19 g/day) was the highest growth rate and the highest feed efficiency in treatment C was 88.03%. Keywords: tilapia, mas snail flour, growth rate, and feed efficiency Abstrak Ikan nila (Oreochromis niloticus) memiliki nilai ekonomis karena permintaan konsumen yang tinggi, harga yang relatif terjangkau, rasa yang digemari dan memiliki toleransi yang tinggi terhadap lingkungan. Kendala yang dihadapi petani adalah harga pakan yang tinggi karena bahan baku seperti tepung ikan masih di impor. Maka perlu mencari bahan baku alternatif seperti tepung keong mas yang memiliki harga yang murah.Tujuan penelitian ini adalah menganalisis proporsi tepung keong mas yang tepat dalam pakan ikan sehingga mampu memberikan laju pertumbuhan yang maksimal bagi benih ikan nila gift. Rancangan percobaan yang digunakan ialah Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dengan 3 ulangan. Parameter yang diamati adalah laju pertumbuhan dan efisiensi pakan. Hasil percobaan menunjukkan bahwa perlakuan C (0,19 g/hari) merupakan laju pertumbuhan yang tertinggi dan efesiensi pakan tertinggi pada perlakuan C yaitu 88,03 %. Kata Kunci: ikan nila, tepung keong mas, laju pertumbuhan, dan efisiensi pakan

scholarly journals New Recombinant Escherichia coli Strain Tailored for the Production of Poly(3-Hydroxybutyrate) from Agroindustrial By-Products

2006 ◽  
Vol 72 (6) ◽  
pp. 3949-3954 ◽  
Author(s):  
Pablo I. Nikel ◽  
Alejandra de Almeida ◽  
Evelia C. Melillo ◽  
Miguel A. Galvagno ◽  
M. Julia Pettinari
Keyword(s):  
Corn Steep Liquor ◽  
Constitutive Expression ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Coli Strain ◽  
Physical Analysis ◽  
Lactose Repressor ◽  
Expression Of Genes ◽  
Steep Liquor ◽  
By Products

ABSTRACT A recombinant E. coli strain (K24K) was constructed and evaluated for poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the control of the lactose operator. K24K does not produce the lactose repressor, ensuring constitutive expression of genes involved in lactose transport and utilization. PHB was efficiently produced by the recombinant strain grown aerobically in fed-batch cultures in a laboratory scale bioreactor on a semisynthetic medium supplemented with the agroindustrial by-products. After 24 h, cells accumulated PHB to 72.9% of their cell dry weight, reaching a volumetric productivity of 2.13 g PHB per liter per hour. Physical analysis of PHB recovered from the recombinants showed that its molecular weight was similar to that of PHB produced by Azotobacter sp. strain FA8 and higher than that of the polymer from Cupriavidus necator and that its glass transition temperature was approximately 20�C higher than those of PHBs from the natural producer strains.

NUTRIENT REQUIREMENTS AND GROWTH CONDITIONS FOR PRODUCTION OF LACTASE ENZYME BY SACCHAROMYCES FRAGILIS1

1970 ◽  
Vol 33 (10) ◽  
pp. 451-455 ◽  
Author(s):  
W. L. Wendorff ◽  
C. H. Amundson ◽  
N. F. Olson
Keyword(s):  
Growth Factors ◽  
Cheese Whey ◽  
Corn Steep Liquor ◽  
Growth Conditions ◽  
Nutrient Requirements ◽  
Lactase Activity ◽  
Optimum Conditions ◽  
Factors Affecting ◽  
Lactose Concentration ◽  
Steep Liquor

Lactase enzyme was produced by Saccharomyces fragilis NRRL Y-1109 grown in deproteinized Cheddar cheese whey. Nutrients and growth conditions required for maximum lactase production were determined. Lactose concentration, sources of growth factors; temperature of incubation, and pH of the whey were the primary factors affecting lactase production. Increased levels of lactose in whey stimulated the yeast to produce higher levels of lactase activity per gram of dried yeast. Corn steep liquor was the best source of added growth factors. Yeast grown under optimum conditions, pH 4.0 to 4.7 and 28 C, yielded 175 units per gram of yeast and 1300 units per liter of whey.

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