scholarly journals First successful hybridization experiment between native European weatherfish (Misgurnus fossilis) and non-native Oriental weatherfish (M. anguillicaudatus) reveals no evidence for postzygotic barriers

NeoBiota ◽  
2021 ◽  
Vol 69 ◽  
pp. 29-50
Author(s):  
Josef Wanzenböck ◽  
Mathias Hopfinger ◽  
Sylvia Wanzenböck ◽  
Lukas Fuxjäger ◽  
Hans Rund ◽  
...  
Keyword(s):  
Divergence Time ◽  
Standard Metabolic Rate ◽  
Hybridization Experiment ◽  
F1 Hybrids ◽  
Hybrid Vigour ◽  
Freshwater Species ◽  
Physiological Basis ◽  
Ploidy Levels ◽  
Reproductive Isolation Mechanisms ◽  
Misgurnus Fossilis

The European weatherfish Misgurnus fossilis (Linnaeus, 1758) is a threatened freshwater species in large parts of Europe and might come under pressure from currently establishing exotic weatherfish species. Additional threats might arise if those species hybridize which has been questioned in previous research. Regarding the hybridization of M. fossilis × M. anguillicaudatus (Cantor, 1842), we demonstrate that despite the considerable genetic distance between parental species, the estimated long divergence time and different ploidy levels do not represent a postzygotic barrier for hybridization of the European and Oriental weatherfish. The paternal species can be easily differentiated based on external pigment patterns with hybrids showing intermediate patterns. No difference in standard metabolic rate, indicating a lack of hybrid vigour, renders predictions of potential threats to the European weatherfish from hybridization with the Oriental weatherfish difficult. Therefore, the genetic and physiological basis of invasiveness via hybridization remains elusive in Misgurnus species and requires further research. The existence of prezygotic reproductive isolation mechanisms and the fertility of F1 hybrids remains to be tested to predict the potential threats of globally invasive Oriental weatherfish species.

scholarly journals Are genetically distinct lizard species able to hybridize? A review

2015 ◽  
Vol 61 (1) ◽  
pp. 155-180 ◽  
Author(s):  
Jitka Jančúchová-Lásková ◽  
Eva Landová ◽  
Daniel Frynta
Keyword(s):  
Reproductive Isolation ◽  
Genetic Divergence ◽  
Divergence Time ◽  
Distinct Species ◽  
F1 Hybrids ◽  
Lizard Species ◽  
And Bisexual ◽  
Reproductive Isolation Mechanisms ◽  
Cyt B Gene ◽  
The Relationship

Abstract Animal species are delimited by reproductive isolation mechanisms (RIMs). Postzygotic RIMs are mainly products of genetic differences and thus their strength increases with elapsed divergence time. The relationship between postzygotic reproductive isolation and genetic divergence, however, differs considerably among major clades of vertebrates. We reviewed the available literature providing empirical evidence of natural and/or experimental hybridization between distinct species of lizards (squamates except snakes). We found that hybridization events are widely distributed among nearly all major lizard clades. The majority of research focuses on parthenogenetic species and/or polyploid hybrids in families Lacertidae, Teiidae and Gekkonidae. Homoploid bisexual hybrids are mainly reported within Lacertidae and Iguania groups. As a proxy of genetic divergence of the hybridizing taxa we adopted nucleotide sequence distance (HKY85) of mitochondrial cyt b gene. The upper limit of genetic divergence was similar with regard to both parthenogenetic and bisexual hybrids. Maximum values of these distances between hybridizing species of lizards approached 18%?21%, which is comparable to or even exceeds the corresponding values reported for other principal clades of vertebrates. In spite of this, F1 hybrids are typically at least partially fertile in lizards and thus genetic in-trogression between highly divergent species is possible. The relationship between the genetic distance and hybrid fertility was not found.

Photoinhibition in indica and japonica subspecies of rice (Oryza sativa) and their reciprocal F1 hybrids

2001 ◽  
Vol 28 (4) ◽  
pp. 299 ◽  
Author(s):  
Demao Jiao ◽  
Benhua Ji
Keyword(s):  
Protein Synthesis ◽  
Oryza Sativa ◽  
Xanthophyll Cycle ◽  
Intrinsic Factor ◽  
D1 Protein ◽  
Physiological Role ◽  
F1 Hybrids ◽  
Protein Synthesis Inhibitor ◽  
Physiological Basis ◽  
Maternal Line

Photoinhibitory performance and its physiological basis have been studied in Oryza sativa L. subspecies indica and japonica, and their reciprocal F1 hybrids. The results demonstrate that the japonica ssp. was usually more tolerant to photoinhibition, indicated by higher maintaining capacity of D1 protein (less degradation), higher induced superoxide dismutase (SOD) activity and longer duration of the activity. Compared with japonica, the indica ssp. was more sensitive to photoinhibition, and exhibited more degradation of D1 protein and a much larger xanthophyll pool. A statistically significant positive correlation exists between D1 protein content and Fv/Fm, PSII activity and Pn during photoinhibition (r2 = 0.98, 0.93, 0.95, respectively, P < 0.01). This result further supported the hypothesis that D1 protein encoded by plastid genes might play an important physiological role in the mechanism of photoinhibition. This hypothesis is also enhanced by the fact that the capacity of D1 protein synthesis mediated the components and cycle of xanthophyll and non-photochemical quenching in treatment with streptomycin, a D1 protein synthesis inhibitor, while the xanthophyll cycle had a photoprotective role for D1 protein and Fv/Fm in treatment with DDT, a xanthophyll cycle inhibitor. The photoinhibition of reciprocal F1 hybrids between japonica and indica is mainly intermediate, but somewhat inclined to the maternal line. This demonstrates that the basic feature of photoinhibition was controlled by the interaction of an intrinsic factor, D1 protein encoded by plastid genes, with the xanthophyll cycle, as well as SOD, controlled by nuclear genes. Since the response to photoinhibitory treatment of indica–japonica F1 hybrids seems to depend on female parents, we propose to select and use photoinhibition-tolerant varieties as female parents for generation of photoinhibition-tolerant hybrids.

Physiological basis of metabolic trade-offs between growth and performance among different strains of rainbow trout

2016 ◽  
Vol 73 (10) ◽  
pp. 1493-1506 ◽  
Author(s):  
David Allen ◽  
Jordan Rosenfeld ◽  
Jeffrey Richards
Keyword(s):  
Rainbow Trout ◽  
Metabolic Rate ◽  
Energy Content ◽  
Environmental Gradients ◽  
High Growth ◽  
Growth Efficiency ◽  
Standard Metabolic Rate ◽  
Physiological Basis ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Trade Offs

Adaptive trade-offs define the trait combinations that differentiate taxa and allow coexistence along environmental gradients. To understand the physiological trade-offs associated with growth, we examined relationships among metabolic rate, digestive capacity, tissue energy content, and growth in juveniles of three strains of rainbow trout (Oncorhynchus mykiss) that differ in growth. Fish were reared under satiation, 1% of body mass per day, and complete food deprivation treatments to assess differences in performance and adaptive trade-offs along a gradient of resource availability. The fast-growing hatchery strain had higher standard metabolic rate (SMR), lower aerobic scope, and potentially lower maximum metabolic rates, suggesting that high growth trades off against a reduced capacity to do metabolic work. Trout with high growth rates also generally had larger gastrointestinal tracts, maximum food consumption, and growth efficiency. Results demonstrate (i) higher SMR of fast growers appears to be related to a greater investment in high-maintenance digestive tissue that supports rapid growth; (ii) growth appears to trade off against active metabolism; and (iii) selection on growth involves a suite of integrated physiological and anatomical traits that are affected by both genotype and environment (ration).

scholarly journals Gene and Transposable Element Expression Evolution Following Recent and Past Polyploidy Events in Spartina (Poaceae)

2021 ◽  
Vol 12 ◽  
Author(s):  
Delphine Giraud ◽  
Oscar Lima ◽  
Mathieu Rousseau-Gueutin ◽  
Armel Salmon ◽  
Malika Aïnouche
Keyword(s):  
Gene Expression ◽  
Transposable Element ◽  
Divergence Time ◽  
Reticulate Evolution ◽  
F1 Hybrids ◽  
Polyploid Evolution ◽  
Gene Expression Dynamics ◽  
Expression Evolution ◽  
Weak Expression ◽  
Spartina Versicolor

Gene expression dynamics is a key component of polyploid evolution, varying in nature, intensity, and temporal scales, most particularly in allopolyploids, where two or more sub-genomes from differentiated parental species and different repeat contents are merged. Here, we investigated transcriptome evolution at different evolutionary time scales among tetraploid, hexaploid, and neododecaploid Spartina species (Poaceae, Chloridoideae) that successively diverged in the last 6–10 my, at the origin of differential phenotypic and ecological traits. Of particular interest are the recent (19th century) hybridizations between the two hexaploids Spartina alterniflora (2n = 6x = 62) and S. maritima (2n = 6x = 60) that resulted in two sterile F1 hybrids: Spartina × townsendii (2n = 6x = 62) in England and Spartina × neyrautii (2n = 6x = 62) in France. Whole genome duplication of S. × townsendii gave rise to the invasive neo-allododecaploid species Spartina anglica (2n = 12x = 124). New transcriptome assemblies and annotations for tetraploids and the enrichment of previously published reference transcriptomes for hexaploids and the allododecaploid allowed identifying 42,423 clusters of orthologs and distinguishing 21 transcribed transposable element (TE) lineages across the seven investigated Spartina species. In 4x and 6x mesopolyploids, gene and TE expression changes were consistent with phylogenetic relationships and divergence, revealing weak expression differences in the tetraploid sister species Spartina bakeri and Spartina versicolor (&lt;2 my divergence time) compared to marked transcriptome divergence between the hexaploids S. alterniflora and S. maritima that diverged 2–4 mya. Differentially expressed genes were involved in glycolysis, post-transcriptional protein modifications, epidermis development, biosynthesis of carotenoids. Most detected TE lineages (except SINE elements) were found more expressed in hexaploids than in tetraploids, in line with their abundance in the corresponding genomes. Comparatively, an astonishing (52%) expression repatterning and deviation from parental additivity were observed following recent reticulate evolution (involving the F1 hybrids and the neo-allododecaploid S. anglica), with various patterns of biased homoeologous gene expression, including genes involved in epigenetic regulation. Downregulation of TEs was observed in both hybrids and accentuated in the neo-allopolyploid. Our results reinforce the view that allopolyploidy represents springboards to new regulatory patterns, offering to worldwide invasive species, such as S. anglica, the opportunity to colonize stressful and fluctuating environments on saltmarshes.

scholarly journals Heterotic Effects in Fls and Inbreeding Depression in F2 Hybrids of Sunflower

2015 ◽  
Vol 58 (1) ◽  
pp. 1-10
Author(s):  
Shahnaz Memon ◽  
Muhammad Jurial Baloch ◽  
Gul Muhammad Baloch ◽  
Muhammad Ibrahim Keerio
Keyword(s):  
Plant Height ◽  
Inbreeding Depression ◽  
Seed Yield ◽  
Block Design ◽  
Oil Yield ◽  
F1 Hybrids ◽  
Hybrid Vigour ◽  
Head Diameter ◽  
Days To Maturity ◽  
F2 Hybrids

Genetically diverse female lines of sunflower were crossed with male testers to get heterotic hybrids. Studies were carried-out during 2008-2010 at Experiment filed of Agriculture Research Institute, Tandojam, Sindh, Pakistan. Six female lines like T-4-0319, PAC-0505, HO-I, Hysun-33, Peshawar-93 and CMS-03 and three testers i.e., PAC-0306, PAC-64-A and SF-187 were crossed in a line ´ tester mating design, thus 18 F1 and F2 hybrids were developed for evaluation of heterosis and inbreeding depression for days to initial flowering, days to maturity, leaves/plant, plant height (cm), head diameter (cm), 1000-achene weight (g), seed yield kg/ha and oil yield kg/ha. The experiment was conducted in a randomised complete block design with four replications. The analysis of variance revealed significant differences among parents, F1s and F2 hybrids for all the traits studied. The existence of significant genetic variability among the plant traits is particularly useful because variations in these traits would allow further improvement in sunflowerseed yield and oil traits. The F1 hybrids HO-I ´ PAC-0306 and HO-I PA ´ C-64-A exhibited desirable negative mid and better parent heterosis for days to initial flowering, days to maturity and plant height. These hybrids also manifested desirable positive heterotic effects for leaves/plant, head diameter, 1000-achene’s weight, seed yield and oil yield. Inbreeding depression for phenological, seed yield and oil traits  showed that desirable high inbreeding depression was observed in hybrids HO-I ´ P´AC-64-A, HO-I ´ PAC-0306 and HO-I ´ SF-187 for days to initial flowering, similarly T-4-0319 ´ PAC-0306, PAC-0505 ´ SF-187 and HO-I ´ PAC-64-A explicated maximum but rewarding inbreeding depression for days to maturity. The F2 hybrids Hysun-33 ´ SF-187 and Peshawer-93 ´ PAC-64-A may be the most desirable ones in the sense that they recorded comparatively moderate inbreeding depression with enough number of leaves to be productive if F2 hybrids are to be exploited for hybrid vigour. Low inbreeding depression for various traitsindicated that such hybrids some how favour the usefulness of F2 hybrids in sunflower. 

scholarly journals Effects of osmotic and thermal shock on the invasive aquatic mudsnail Potamopyrgus antipodarum: mortality and physiology under stressful conditions

NeoBiota ◽  
2020 ◽  
Vol 54 ◽  
pp. 1-22
Author(s):  
Esteban M. Paolucci ◽  
Erik V. Thuesen
Keyword(s):  
Environmental Temperature ◽  
High Salinity ◽  
Standard Metabolic Rate ◽  
Potamopyrgus Antipodarum ◽  
Enzymatic Activities ◽  
Freshwater Species ◽  
Metabolic Rates ◽  
Acclimation Period ◽  
Physiological Tolerances ◽  
Harsh Conditions

Invasive freshwater species, such as the exotic mollusc Potamopyrgus antipodarum (New Zealand mudsnail), can frequently survive under harsh conditions, including brackish and hypoxic environments. We experimentally assessed the effects of osmotic (0, 10, 20, 25 and 30 psu) and thermal (20 °C) shock on mortality, activity and physiology of P. antipodarum collected at Capitol Lake, Olympia, Washington, USA, during winter and spring seasons when environmental temperature was 5 and 10 °C respectively. We measured standard metabolic rate and enzymatic activities (malate dehydrogenase, lactate dehydrogenase, alanopine dehydrogenase) in snails after a 10-day acclimation period at high salinity. Significantly higher mortalities were observed at higher salinities; the strongest effects occurred on snails collected at the end of winter, and exposed to 30 psu and 20 °C (100% mortality in 3 days). When snails were collected during the spring, 100% mortality was observed after 40 days at 30 psu and 20 °C. Standard metabolic rates were significantly lower when snails were exposed to salinities of 25 and 30 psu, even after 10 days of acclimation. Enzymatic activities showed small but significant declines after 10 days at 30 psu reflecting the declines observed in overall metabolism. The physiological tolerances to temperature and salinity displayed by this population of P. antipodarum make its eradication from Capital Lake difficult to achieve.

scholarly journals Mitotic and meiotic studies on two species of Ocimum (Lamiaceae) and their F1 hybrids

2017 ◽  
Vol 23 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Johnson Ademola Idowu ◽  
Matthew Oziegbe
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Interspecific Variation ◽  
F1 Hybrids ◽  
Root Tips ◽  
Flower Buds ◽  
Ploidy Levels ◽  
Ocimum Species ◽  
Karyotype Formula ◽  
Standard Techniques

AbstractOcimum L. species are important aromatic and medicinal plants. Many researchers have observed complexity in their chromosome numbers and ploidy levels. We studied the somatic and germline chromosomal features and behaviour of Ocimum basilicum L., two variants of O. canum Sims (‘c1’ and ‘c2’) and their F1hybrids. Chromosomes from root tips and flower buds were investigated using standard techniques; karyograms were formed and analysed. A chromosome number of 2n = 4x = 52 was observed in O. basilicum and O. canum ‘c1’ and their F1hybrid. One of the variants, O. canum ‘c2’ had a chromosome number of 2n = 2x = 24 and its intraspecific hybrid O. canum ‘c2’ × O. canum ‘c1’ had a chromosome number of 2n = 38. These Ocimum species and their F1hybrids showed different karyotype formula, but their chromosomes were mostly metacentric (174) and submetacentric (36) with few subtelocentric (8). Based on pairing configuration, O. basilicum is an allotetraploid plant, O. canum ‘c1’ is an autotetraploid plant and the O. canum ‘c1’ is a diploid. The F1 hybrids showed higher frequency of meiotic abnormalities than the parents. The study showed intraspecific and interspecific variation in chromosome numbers and pairing patterns, but the chromosomes of the Ocimum species were similar in their centromeric positions.

scholarly journals HYBRID VIGOUR EFFECT IN SORGHUM SELECTION

2019 ◽  
pp. 15-24
Author(s):  
O. P. Kibalnik
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
First Generation ◽  
Abiotic Factors ◽  
F1 Hybrids ◽  
Hybrid Vigour ◽  
Parental Form ◽  
Average Value ◽  
Dominant Genes ◽  
Inflorescence Length

The authors see application of hybrid vigour in the hybrids obtained on the basis of mother lines with cytoplasmic male sterility as a promising way of sorghum selection. The selection is aimed at increasing sorghum productivity. Hybrid vigour is observed in the first generation of hybrids which was caused by interaction among genes (dominance and epistasis) and additive effect of dominant genes. Its effect is weakened in further generations. The paper explores the effect of heterosis of F1 hybrids obtained on the basis of CMC lines with A1, A2, A3, A4, M-35-1A, 9E types of sterility and productive pollinator varieties which are resistant to biotic and abiotic factors in the region. The experiment was carried out on the pilot plots of Research and Technological Institute of Sorghum and Corn “Rossorgo” in 2015-2016. The area of the plot was7.7 m2; frequency observed was three times; landfill location was random. The density of new hybrids, standards and parental forms was 100 thousand plants pro a hectare. The frequency of pure (superior to the superior parental form), hypothetical (superior to the average value of parental forms) and competitive (excess of the sign of hybrid F1 over the released hybrid) heterosis in terms of: plant height, inflorescence length, grain mass per panicle and 1000 grains, yield. Heterosis was more frequent in terms of plant height, inflorescence length, weight of 1000 grains, and less frequently (mainly in 2016) in terms of yield and weight of grain from a panicle. The authors indicate the prospective combinations of crosses with low effect of competitive heterosis in plant height and the highest yield as: A1 O-Yang 1/Avance, A1 O-Yang 1/Topaz, A3 Feterita 14/Mercury, A4 KP 70/Volzhskoe 4. These hybrids form the grain yield of 4.09-9.15 t/ha (2015-2016) and are characterized by the effect of competitive heterosis in2016 interms of grain yield from 2.1 to 71.4%. Hybrids A1 O-Yang 1/Volzhskoe 4 and A2 KVV 114/Avance differ in competitive heterosis by weight of 1000 grains (51.7-60.0%) and grains from one panicle (5.8-52.9%). The hybrids outlined are expected to be relevant for further tests.

scholarly journals Evaluation of the courtship and of the hybrid male sterility among Drosophila buzzatii cluster species (Diptera, Drosophilidae)

2002 ◽  
Vol 62 (4a) ◽  
pp. 601-608 ◽  
Author(s):  
L. P. de B. MACHADO ◽  
J. P.de CASTRO ◽  
L. MADI-RAVAZZI
Keyword(s):  
Reproductive Isolation ◽  
Male Sterility ◽  
F1 Hybrids ◽  
Interspecific Crosses ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Drosophila Buzzatii ◽  
Repleta Group ◽  
Reproductive Isolation Mechanisms ◽  
Isolation Mechanisms

In the Drosophila repleta group the establishment of subgroups and complexes made on the basis of morphological and cytological evidences is supported by tests of reproductive isolation. Among species in the repleta group, the buzzatii cluster, due to its polymorphism and polytipism, is an excellent material for ecological and speciation studies. Some interspecific crosses involving Drosophila seriema, Drosophila sp. B, D. koepferae and D. buzzatii strains were completely sterile while others involving strains from these species produced F1 hybrids that did not yield F2. In the present work, data on courtship duration and copula occurrence obtained in the analysis of flies from parental sterile crosses and on spermatozoon mobility observed in F1 hybrids that did not yield F2 are presented. Copula did not occur during one hour of observation and the spermatozoon also did not show mobility at any of the analyzed stages (3, 7, 9 and 10 days old). There was a high variation in courtship average duration and in the percentage of males that courted the females. The reproductive isolation mechanisms indicated by these observations were pre and post-zygotic, as supported by the absence of copula and male sterility. Data obtained also showed the occurrence of different degrees of reproductive compatibility among the strains classified as the same species but from distinct geographic localities.

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