scholarly journals Survival of the copepod Mesocyclops longisetus during simulations of transport from hatchery to target areas for biological control of mosquito larvae

2020 ◽  
Vol 37 ◽  
pp. 1-4
Author(s):  
Aline Nunes-Silva ◽  
Isabela de Lima-Keppe ◽  
Robson V. de Souza ◽  
Gilmar Perbiche-Neves
Keyword(s):  
Biological Control ◽  
Mortality Rate ◽  
Aedes Aegypti ◽  
Mosquito Larvae ◽  
Transportation Time ◽  
Transportation Times

Copepods have been successfully used in many countries for the biological control of larvae of mosquitoes that vector diseases. In Brazil, this line of research has been focused on the use of the copepod Mesocyclops longisetus (Thiébaud, 1914) for the biological control of the mosquito Aedes aegypti (Linnaeus, 1792). The transportation of the copepods from the place where they are reared to where they will be used often involves long distances for extended periods of time. This study assesses the survivorship of M. longisetus during simulation of transport under different conditions. Different loading densities (20, 30, 40, 80, and 120 ind.L) and stirring times (30 minutes, one hour, two hours, and four hours) were tested. Survivorship was high, with 75% of the results equal or higher than 90% survival. Reduced mortality was observed when transportation time was up to 120 minutes and densities were up to 40 ind.L. In higher densities or longer transportation times, the mortality rate was significantly affected.

scholarly journals Uji Efektivitas Bacillus thuringiensis var. Israelensis yang Telah Kedaluwarsa Terhadap Larva Nyamuk Aedes aegypti

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Novia Kurnia Sari ◽  
E Setyaningrum ◽  
Emantis Rosa
Keyword(s):  
Bacillus Thuringiensis ◽  
Mortality Rate ◽  
Aedes Aegypti ◽  
Contact Time ◽  
Positive Control ◽  
Negative Control ◽  
Natural Sciences ◽  
Effective Concentration ◽  
Mosquito Larvae ◽  
Main Factor

ABSTRAKPenelitian ini bertujuan untuk mengetahui konsentrasi efektif dan mortalitas Bti kedaluwarsa terhadap larva nyamuk Ae. aegypti. Penelitian ini dilaksanakan pada bulan Desember 2018 di Laboratorium Zoologi II, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung. Rancangan penelitian yaitu RAL (Rancangan Acak Lengkap) menggunakan Bti kedaluwarsa sebagai faktor utama dengan 6 taraf konsentrasi : 0 ppm (kontrol positif), 50 ppm, 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm dan Bti normal 20 ppm sebagai kontrol negatif yang terdiri dari 4 ulangan. Parameter yang di uji adalah tingkat mortalitas larva Ae. aegypti. Data di olah dengan uji ANOVA dan dilanjut uji BNT pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa konsentrasi Bti kedaluwarsa 300 ppm merupakan konsentrasi Bti kedaluwarsa yang paling tinggi dan lamanya waktu kontak yang paling cepat menyebabkan kematian larva Ae. aegypti hingga 100%. Kesimpulannya Bti kedaluwarsa masih efektif dan memiliki pengaruh terhadap kematian larva Ae. aegypti. Kata kunci : Ae. aegypti , Bacillus thuringiensis var. Israelensis, Mortalitas ABSTRACTThis study aims to determine the effective concentration and mortality of Bti who have expired mosquito larvae Ae. aegypti. This research was conducted in December 2018 at the Zoology Laboratory II, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This research was conducted with RAL (Complete Random Design) using Bti expiration as the main factor with 6 levels of concentration: 0 ppm (positive control), 50 ppm, 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm and normal Bti 20 ppm as a negative control consisting of 4 replications. As a parameter is the mortality rate of larvae Ae. aegypti. Data was analyzed using ANOVA test and continued with LSD test at the 5% level. The results showed that the Bti concentration expiry of 300 ppm the highest expiration Bti concentration and the longest contact time to cause death to the larvae Ae. aegypti up to 100%. In conclusion, the Bti expired has an effective and influence on the death of the larvae Ae. aegypti. Keywords: Ae. aegypti, Bacillus thuringiensis var. Israelensis, Mortality

Larvicidal Activity of Entomopathogenic Nematodes Isolated from Mexico against Aedes aegypti (Diptera: Culicidae)

2021 ◽  
Vol 56 (1) ◽  
pp. 12-23
Author(s):  
Diego Treviño-Cueto ◽  
Sergei A. Subbotin ◽  
Sergio R. Sanchez-Peña
Keyword(s):  
Biological Control ◽  
Aedes Aegypti ◽  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Horizontal Transmission ◽  
Fourth Instar ◽  
Mosquito Larvae ◽  
Biological Control Agents ◽  
Median Lethal Concentration ◽  
Mosquito Management

Abstract Entomopathogenic nematodes (EPNs) are widely used agents of biological control, mainly targeting soil-inhabiting insect pests. Reports indicate that these terrestrial EPNs are also able to infect the aquatic larvae of mosquitoes. We isolated EPN strains (Heterorhabditis bacteriophora Poinar and Steinernema carpocapsae [Weiser]) from local soils at Saltillo, Coahuila state, Mexico. EPNs from these strains were produced in the laboratory in yellow mealworm (Tenebrio molitor L.) larvae, and their pathogenicity as infective juveniles (IJs) was tested against larvae of the yellow fever mosquito Aedes aegypti (L.) Third- and fourth-instar mosquito larvae were exposed to four concentrations of IJs (25, 50, 100, and 200 IJ/larva) of five strains of local EPNs in laboratory assays. All strains of EPN caused lethal infections in larvae (3–100%); in particular, strain M5 of S. carpocapsae caused 100% mortality at the 200 IJ/larva concentration, with a median lethal concentration (LC50) of 42 IJ/larva (LC90 = 91 IJ/larva). Strain M18 of H. bacteriophora caused 73% mortality at 200 IJ/larva, with an LC50 = 72 and LC90 = 319 IJ/larva. IJs were produced by all strains in mosquito larvae, with a range of 66–239 IJ/mosquito larva (inoculated at 100 IJ/larva) across strains, suggesting that horizontal transmission might occur in the field. This represents the first report of native EPN strains from Mexico exhibiting pathogenicity against mosquito larvae. Native EPN strains should be further evaluated as potential biological control agents in mosquito management.

Survival of Larvivorous Fish Used for Biological Control of Aedes aegypti Larvae in Domestic Containers With Different Chlorine Concentrations

2009 ◽  
Vol 46 (4) ◽  
pp. 841-844 ◽  
Author(s):  
Luciano Pamplona de Góes Cavalcanti ◽  
Francisco José de Paula ◽  
Ricardo José Soares Pontes ◽  
Jorg Heukelbach ◽  
José Wellington de Oliveira Lima
Keyword(s):  
Biological Control ◽  
Aedes Aegypti ◽  
Larvivorous Fish

scholarly journals Observations on the predatory potential of Lutzia fuscana on Aedes aegypti larvae: implications for biological control (Diptera: Culicidae)

2016 ◽  
Vol 48 (2) ◽  
pp. 137
Author(s):  
Soujita Pramanik ◽  
Sampa Banerjee ◽  
Soumyajit Banerjee ◽  
Goutam K. Saha ◽  
Gautam Aditya
Keyword(s):  
Biological Control ◽  
Aedes Aegypti ◽  
Biological Control Agent ◽  
Instar Larva ◽  
Control Agent ◽  
The Body ◽  
Larval Habitats ◽  
Larval Stages ◽  
Augmentative Release ◽  
Predatory Potential

Among the natural predators, larval stages of the mosquito <em>Lutzia fuscana (</em>Wiedemann, 1820) (Diptera: Culicidae) bear potential as a biological control agent of mosquitoes. An estimation of the predatory potential of the larva of <em>L. fuscana</em> against the larva of the dengue vector <em>Aedes aegypti</em> (Linnaeus, 1762) (Diptera: Culicidae) was made to highlight its use in vector management. Laboratory experiments revealed that the larva of<em> L</em>. <em>fuscana</em> consumes 19 to 24 <em>A. aegypti</em> larvae per day, during its tenure as IV instar larva. The consumption of <em>A. aegypti</em> larvae was proportionate to the body length (BL) and body weight (BW) of the predatory larva<em> L. fuscana</em> as depicted through the logistic regressions: y = 1 / (1 + exp(-(-2.09 + 0.35*BL))) and y = 1 / (1 + exp(-(0.4+ 0.06*BW))). While the prey consumption remained comparable among the days, the net weight gained by the <em>L</em>. <em>fuscana</em> larva showed a decreasing trend with the age. On the basis of the results, it is apparent that the larva of the mosquito <em>L. fuscana</em> can be used in the regulation of the mosquito <em>A. aegypti</em> through augmentative release, particularly, in the smaller mosquito larval habitats.

scholarly journals LARVICIDAL ACTIVITY OFTITHONIA DIVERSIFOLIALEAF EXTRACT ONPERI-URBAN MOSQUITO LARVEA INABAKALIKI; ALTERNATIVE TO INSECTICIDE DEVELOPMENT.

2015 ◽  
Vol 7 (1) ◽  
pp. 1225-1228
Author(s):  
UHUO CA ◽  
OKEREKE CN ◽  
NWELE ED ◽  
OGBONNA S.U ◽  
NWANCHOR K.C ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Adverse Effects ◽  
Bioactive Compounds ◽  
Larvicidal Activity ◽  
Urban Areas ◽  
Insect Pests ◽  
Anopheles Mosquito ◽  
Mosquito Population ◽  
Mosquito Larvae ◽  
Dose Dependent

The bioassay activitiesofTithonia diversifolia leave extract was conducted on the larvae of Anopheles mosquito collected at peri-urban areas of Abakaliki Ebonyi State, using the concentrations of the extract in dilutions at 50/100ml, 40/100ml, 30/100ml and 20/100ml introduced with 10 Anopheles mosquito larvae each in four replicates and allowed for 3hrs. Mean mortality rate of the larvae were observed after the first hour, thus 30%, 10%, 05% and 0% respectively while in the 2nd hour were 60%, 40%, 20% and 10% and in the 3rd hour were 80%, 60%, 50% & 30% respectively. The result thus revealed that the treatment is dose dependent and that the studied specie has some bioactive compounds that can be exploited for insect pests control hence observed to be sensitive in anopheles mosquito larvae. Therefore Tithonia diversifolialeaf extract could be used as a bioassay for the control of mosquito due to its active properties as this has exhibited adverse effects on the larvae thereby reducing the mosquito population and thus reducing the malarious infection associated with the bite of mosquito.

scholarly journals Effects of two formulations based on Bacillus thuringiensis subsp. israelensis on Aedes aegypti mosquito larvae under laboratory conditions

2008 ◽  
Vol 23 (4) ◽  
pp. 251-258
Author(s):  
Milenka Peric ◽  
Mirjana Prijovic ◽  
Goran Andric
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Aqueous Suspension ◽  
Pure Water ◽  
Clay Content ◽  
Experimental Period ◽  
Mosquito Larvae ◽  
Water Dispersible ◽  
Bacillus Thuringiensis Subsp ◽  
Zero Time

Toxicity and persistence of two formulations based on Bacillus thuringiensis subsp. israelensis applied to Aedes aegypti mosquito larvae were tested under laborabory conditions. The formulations were: a) water dispersible granules (product VectoBac WDG), and b) aqueous suspension (product VectoBac 12AS). The effects of both products on mosquito larvae were tested immediately after their dilution in pure water (zero time) and in 1-, 2-, 8- and 13-day old solutions. The two products were also tested in mixtures of water and clay at a rate of 0.5 g clay/150 ml water immediately after product dilution, and in one-day old solutions containing 0.1 and 0.05 g of clay in the same amount of water. The product VectoBac WDG was persistent and highly effective against Ae. aegypti larvae in pure water after the longest experimental period of 13 days, and significantly more effective than VectoBac 12AS at equal rates of application. The effectiveness of VectoBac 12AS weakened significantly after 8 and 13 days of treatment, compared to the effects at zero time and in 1- and 2-day old solutions. High clay content in water significantly reduced the larvicidal effectiveness of both products, indicating its possible compromising role during product application in practice.

scholarly journals Bioefficacy of some insect growth regulators and plant extracts against mosquito larvae of Aedes aegypti

2019 ◽  
Vol 6 (1) ◽  
pp. 001-006
Author(s):  
Mohamd R. Al-Zahrani ◽  
Jazem A. Mahyoub ◽  
Khalid M. Al-Ghamdi ◽  
Habeeb M. Al-Solami
Keyword(s):  
Aedes Aegypti ◽  
Growth Regulators ◽  
Plant Extracts ◽  
Mosquito Larvae ◽  
Insect Growth Regulators ◽  
Insect Growth

scholarly journals The Toxicity of Srikaya Seed Granules (Annona squamosa L.) with Different Heating Temperatures Against the Larva of Aedes aegypti L.

BIOEDUKASI ◽  
2020 ◽  
pp. 1
Author(s):  
Rima Gloria Purwanto ◽  
Dwi Wahyuni ◽  
Joko Waluyo
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Heating Temperature ◽  
Probit Analysis ◽  
Mosquito Larvae ◽  
Annona Squamosa ◽  
Forest Region ◽  
Randomized Design ◽  
Significant Difference ◽  
Lower Temperature

Abstract: Aedes aegypti L. is a mosquito carrying dengue virus that causes dengue fever, especially in Southeast Asia which is a tropical rain forest region which is a habitat for mosquito growth. Aedes aegypti L. mosquito control is carried out by chemical means of fogging and using abate but this control actually causes Aedes aegypti L. mosquitoes to become resistant or immune to chemical drugs so that a new breakthrough is created by making bioinsecticides biologically to eradicate the Aedes aegypti L. mosquito. with characteristics that are environmentally friendly and do not kill non-target animals and cause the Aedes aegypti L. mosquito not to become resistant. To be able to realize this desire so that the sugar cane granules extract of Annona squamosa L. containing active compounds in the form of annonain and squamosin so that they are toxic to Aedes aegypti L. mosquito larvae. Further research is to test the heating temperature level of the granules of Annona squamosa L. extract temperature of 40 ° C and 60 ° C which is more deadly of Aedes aegypti L. mosquito larvae. This research method uses a completely randomized design with four repetitions. The temperature of 60 ° C uses concentrations of 1 ppm, 6 ppm, 12 ppm, 18 ppm, 24 ppm and 30 ppm. As for the temperature of 40 ° C using concentrations of 1 ppm, 7 ppm, 14 ppm, 21 ppm, 28 ppm, and 35 ppm, each temperature compared with aquadest and abate. Data analyzed using probit analysis to determine the LC50, then followed by a statistical test paired sample T-test with SPSS to find out a significant difference between heating temperatures of 40 ° C to 60 ° C. These results then show that a higher temperature of 60 ° C has a higher level of toxicity compared to a lower temperature of 40 ° C.

scholarly journals Glyphosate impairs learning in Aedes aegypti mosquito larvae at field-realistic doses

2018 ◽  
Vol 221 (20) ◽  
pp. jeb187518 ◽  
Author(s):  
Hugo Baglan ◽  
Claudio R. Lazzari ◽  
Fernando J. Guerrieri
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Larvae
Sign in / Sign up

Export Citation Format

Share Document