Probiotic Bifidobacterium lactis, anti-oxidant vitamin E/C and anti-inflammatory dha attenuate lung inflammation due to pm2.5 exposure in mice

2019 ◽  
Vol 10 (1) ◽  
pp. 69-75 ◽  
Author(s):  
C. Panebianco ◽  
F. Bou Nasser Eddine ◽  
G. Forlani ◽  
G. Palmieri ◽  
L. Tatangelo ◽  
...  
Keyword(s):  
Air Pollution ◽  
Vitamin C ◽  
Lung Inflammation ◽  
Expression Profiles ◽  
In Vivo Model ◽  
Docosahexanoic Acid ◽  
Pollution Effects ◽  
Bifidobacterium Lactis ◽  
Anti Oxidant ◽  
Anti Inflammatory

The incidence of asthma and allergic diseases of the airways is constantly increasing, both in the industrialised and developing countries, due to harmful and excessive quantities of air pollution. Although some studies have shown an effect of dietary supplementation of specific nutrients (especially with anti-oxidant and anti-inflammatory properties) in reducing airways inflammatory response, the results are not yet conclusive and the science is still at its infancy. Our hypothesis is that combining such nutrients could provide more benefits than using them alone. The aim of the research project proposed here is to investigate whether specific combinations of nutrients (docosahexanoic acid, vitamin C and E, and Bifidobacterium lactis strain BB-12®, included in an engineered diet) can act synergistically to reduce inflammation given by high level of air pollution. Beside the role of docosahexanoic acid, vitamins C and E on airways inflammatory disease, no study examined the effect of the supplementation of this probiotic strain in pathological conditions caused by air pollution so far. Herein we used a well-established in vivo model for the study of pollution effects, which consists in female BALB/c mice receiving by pharyngeal aspiration either a sham or a particulate matter with diameter <2.5 μm (PM 2.5) containing aerosol. Before treatment, mice were fed either a chow or a supplemented diet. By performing histological analyses and gene expression profiles on lung sections and serum measurement of the cytokine interleukin 10, we found that a specific combination of all the aforementioned nutrients rather than nutrients alone had a synergistic protective effect against PM2.5-induced inflammation. In conclusion, our study support that a supplemental nutritional intervention based on a combination of the probiotic B. lactis BB-12, the anti-oxidant vitamin C and E, and the anti-inflammatory docosahexanoic acid represents a rational option for alleviating air pollution-related lung inflammation.

Genome-wide transcriptional profiling of mononuclear phagocytes recruited to mouse lungs in response to alveolar challenge with the TLR2 agonist Pam3CSK4

2009 ◽  
Vol 297 (4) ◽  
pp. L608-L618 ◽  
Author(s):  
Maciej Cabanski ◽  
Jochen Wilhelm ◽  
Zbigniew Zasłona ◽  
Mirko Steinmüller ◽  
Ludger Fink ◽  
...  
Keyword(s):  
Lung Inflammation ◽  
Expression Profiles ◽  
Dynamic Change ◽  
Gene Expression Profiles ◽  
Mononuclear Phagocytes ◽  
Mrna Levels ◽  
Time Points ◽  
Proinflammatory Mediators ◽  
Anti Inflammatory

Compared with the Toll-like receptor 4 (TLR4) ligand LPS restricted to Gram-negative bacteria, few studies have addressed induction of lung inflammation and concomitant leukocyte recruitment in response to TLR2 ligands. This study is the first report showing that selective TLR2 stimulation by its ligand Pam3-Cys-Ser-Lys-Lys-Lys-Lys-OH (Pam3CSK4) within the alveolar compartment promoted lung inflammation in mice and induced the migration of circulatory immune cells including mononuclear phagocytes into the inflamed alveolar space. By using the transgenic CX3CR1+/GFP mouse strain for high-purity sorting of circulating and alveolar recruited mononuclear phagocytes together with SMART preamplification and whole genome oligonucleotide microarray techniques, we found that alveolar trafficking of mononuclear phagocytes was associated with profound changes of their gene expression profiles (∼900 differentially regulated genes postrecruitment). In particular, alveolar recruited mononuclear phagocytes showed upregulated transcripts of genes encoding cytokines/chemokines and pattern recognition receptor (PRR)-associated molecules. Notably, we observed a dynamic change of the genetic program of recruited mononuclear phagocytes obtained from bronchoalveolar lavage fluid at different time points (24 vs. 48 h) post-Pam3CSK4 challenge. In early alveolar recruited mononuclear phagocytes, mRNA levels of both proinflammatory (e.g., TNF-α, CCL2, and IL-6) and central anti-inflammatory/ proresolution [e.g., IL-1-receptor antagonist (IL-1RN), CD200 receptor (CD200R), IL-1 receptor-associated kinase (IRAK-M), IL-10, and Bcl-2-associated X protein (Bax)] mediators were found to be highly upregulated simultaneously. In corresponding cells recruited until later time points, transcript levels of anti-inflammatory/proresolution molecules persisted at the same level, whereas mRNA levels of proinflammatory mediators were found to decline. Collectively, our in vivo study identifies genetic programs by which alveolar recruited mononuclear phagocytes may contribute to the development and termination of pneumonia caused by Gram-positive bacteria.

scholarly journals Nutraceutical Analysis of Marticaria recutita (Chamomile) Dried Leaves and Flower Powder and Comparison between Them

2018 ◽  
Vol 10 (2) ◽  
pp. 111
Author(s):  
Ekta Singh Chauhan ◽  
Jaya Aishwarya
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Nutrient Content ◽  
Different Types ◽  
Anti Oxidant ◽  
Chamaemelum Nobile ◽  
Anti Inflammatory

<p>Chamomile is known as German Chamomile (Marticaria recutita) and Roman Chamomile (Chamaemelum nobile) a very famous daisy plant. The work mainly focuses on the nutraceuticals potential of Chamomile leaf and flower of this plant. The nutrient contains of the leaf and flower power was determined by various methods. The phytochemicals screening of the leaf and flower aqueous extract was performed by the different procedure. Leaf of this plant is rich in carbohydrate, protein, fat and also rich in vitamin C, iron, zinc and calcium. Whereas flower is rich in moisture and fiber as compared to leaf. The aqueous extract of leaf of Chamomile showed the presence of steroids, terpenoids, flavonoids, tannins and saponins and flower were lacked in alkaloids, saponins, gelatin and phenolic compounds. The results record that leaf and flowers powder contains different types of nutrients and phytochmicals in it.<strong> </strong>Chamomile is rich in different bioactive compounds, antioxidant and phytochemicals; carries many pharmacological and traditional properties. Leaves, flowers and stems of Chamomile are used as anti-oxidant, analgesic, anti-viral, anti-inflammatory, anti-septic, anti-diabetic, anti-proliferative, anti-bacterial activities and many more diseases.<strong> </strong>This paper put a light on nutrient content and phytochemical properties of Chamomile leaf and flower.</p>

scholarly journals FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 126-131
Author(s):  
ND Rekha ◽  
Dattatri K. Nagesha ◽  
PH Rajasree ◽  
N Shruthi
Keyword(s):  
Zeta Potential ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vivo Model ◽  
Particle Size Range ◽  
Nitric Oxide Radical ◽  
Anti Oxidant ◽  
Anti Inflammatory

The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene.  Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent. Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).

scholarly journals Endogenous itaconate is not required for particulate matter-induced NRF2 expression or inflammatory response

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Kaitlyn A Sun ◽  
Yan Li ◽  
Angelo Y Meliton ◽  
Parker S Woods ◽  
Lucas M Kimmig ◽  
...  
Keyword(s):  
Air Pollution ◽  
Particulate Matter ◽  
Inflammatory Response ◽  
Lung Inflammation ◽  
Mitochondrial Enzyme ◽  
Nrf2 Activation ◽  
Anti Inflammatory ◽  
Nrf2 Expression

Particulate matter (PM) air pollution causes cardiopulmonary mortality via macrophage-driven lung inflammation; however, the mechanisms are incompletely understood. RNA-sequencing demonstrated Acod1 (Aconitate decarboxylase 1) as one of the top genes induced by PM in macrophages. Acod1 encodes a mitochondrial enzyme that produces itaconate, which has been shown to exert anti-inflammatory effects via NRF2 after LPS. Here, we demonstrate that PM induces Acod1 and itaconate, which reduced mitochondrial respiration via complex II inhibition. Using Acod1-/- mice, we found that Acod1/endogenous itaconate does not affect PM-induced inflammation or NRF2 activation in macrophages in vitro or in vivo. In contrast, exogenous cell permeable itaconate, 4-octyl itaconate (OI) attenuated PM-induced inflammation in macrophages. OI was sufficient to activate NRF2 in macrophages; however, NRF2 was not required for the anti-inflammatory effects of OI. We conclude that the effects of itaconate production on inflammation are stimulus-dependent, and that there are important differences between endogenous and exogenously-applied itaconate.

scholarly journals Endogenous itaconate is not required for particulate matter-induced NRF2 expression or inflammatory response

2020 ◽  
Author(s):  
Kaitlyn A. Sun ◽  
Yan Li ◽  
Angelo Y. Meliton ◽  
Parker S. Woods ◽  
Lucas M. Kimmig ◽  
...  
Keyword(s):  
Air Pollution ◽  
Particulate Matter ◽  
Inflammatory Response ◽  
Rna Sequencing ◽  
Mitochondrial Respiration ◽  
Lung Inflammation ◽  
Mitochondrial Enzyme ◽  
Nrf2 Activation ◽  
Anti Inflammatory ◽  
Nrf2 Expression

ABSTRACTParticulate matter (PM) air pollution causing significant cardiopulmonary mortality via macrophage-driven lung inflammation; however, the mechanisms are not completely understood. RNA-sequencing demonstrated Acod1 (Aconitate decarboxylase 1) as one of the top genes induced by PM in macrophages. Acod1 encodes a mitochondrial enzyme that produces itaconate, which has been shown to exert anti-inflammatory effects via NRF2 after LPS. Here, we demonstrate that PM induces Acod1 and itaconate, which reduced mitochondrial respiration via complex II inhibition. Using Acod1-/- macrophages, we found that Acod1/endogenous itaconate was not required for PM-induced inflammation or NRF2 activation. In contrast to endogenous itaconate, exogenous cell permeable form of itaconate (4-octyl itaconate (OI)) attenuated the PM-induced inflammation and activated NRF2 but NRF2 was not required for the anti-inflammatory effects of OI. We conclude that the effects of itaconate production on inflammation are stimulus-dependent, and that there are important differences between endogenous and exogenously-applied itaconate.

scholarly journals C-peptide, a novel inhibitor of lung inflammation following hemorrhagic shock

2011 ◽  
Vol 300 (5) ◽  
pp. L730-L739 ◽  
Author(s):  
Ranjit S. Chima ◽  
Timberly LaMontagne ◽  
Giovanna Piraino ◽  
Paul W. Hake ◽  
Alvin Denenberg ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Hemorrhagic Shock ◽  
Plasma Levels ◽  
Lung Inflammation ◽  
Biological Effects ◽  
Ischemia Reperfusion ◽  
In Vivo Model ◽  
Inflammatory Signaling ◽  
C Peptide ◽  
Anti Inflammatory

C-peptide is a 31-amino acid peptide cleaved from proinsulin during insulin synthesis. Initially thought to be inert, C-peptide may modulate the inflammatory response in the setting of endotoxemia and ischemia reperfusion. However, the spectrum of its biological effects is unclear. We hypothesized that exogenous administration of C-peptide would modulate pro- and anti-inflammatory signaling pathways and thereby attenuate lung inflammation in an in vivo model of hemorrhagic shock. Hemorrhagic shock was induced in male Wistar rats (aged 3–4 mo) by withdrawing blood to a mean arterial pressure of 50 mmHg. At 3 h after hemorrhage, rats were rapidly resuscitated by returning their shed blood. At the time of resuscitation and every hour thereafter, animals received C-peptide (280 nmol/kg) or vehicle parenterally. Animals were euthanized at 1 and 3 h after resuscitation. C-peptide administration at resuscitation following hemorrhagic shock ameliorated hypotension and blunted the systemic inflammatory response by reducing plasma levels of IL-1, IL-6, macrophage inflammatory protein-1α, and cytokine-induced neutrophil chemoattractant-1. This was associated with a reduction in lung neutrophil infiltration and plasma levels of receptor for advanced glycation end products. Mechanistically, C-peptide treatment was associated with reduced expression of proinflammatory transcription factors activator protein-1 and NF-κB and activation of the anti-inflammatory transcription factor peroxisome proliferator-activated receptor-γ. Our data suggest that C-peptide ameliorates the inflammatory response and lung inflammation following hemorrhagic shock. These effects may be modulated by altering the balance between pro- and anti-inflammatory signaling in the lung.

scholarly journals Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 139 ◽  
Author(s):  
Vafa Baradaran Rahimi ◽  
Hassan Rakhshandeh ◽  
Federica Raucci ◽  
Benedetta Buono ◽  
Reza Shirazinia ◽  
...  
Keyword(s):  
Lung Injury ◽  
Lung Inflammation ◽  
Transforming Growth Factor ◽  
White Blood Cells ◽  
Portulaca Oleracea ◽  
Inflammatory Activity ◽  
Protective Agent ◽  
Tnf Α ◽  
Anti Oxidant ◽  
Anti Inflammatory

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are classified as two lung complications arising from various conditions such as sepsis, trauma, and lung inflammation. Previous studies have shown that the extract of the leaves of Portulaca oleracea (PO) possesses anti-inflammatory and anti-oxidant activities. In the present study, the effects of PO (50–200 mg/kg) and dexamethasone (Dexa; 1.5 mg/kg) on lipopolysaccharide (LPS)-induced ALI were investigated. Subsequentially, the lung wet/dry ratio; white blood cells (WBC); levels of nitric oxide (NO); myeloperoxidase (MPO); malondialdehyde (MDA); thiol groups formation; super oxide dismutase (SOD) and catalase (CAT) activities; and levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, IL-10, prostaglandin E2 (PGE2), and transforming growth factor (TGF)-β in the broncho alveolar lavage fluid (BALF) were evaluated in order to demonstrate the anti-oxidant and anti-inflammatory activity of PO. Our results show that PO suppresses lung inflammation by the reduction of IL-β, IL-6, TNF-α, PGE2, and TGF-β, as well as by the increase of IL-10 levels. We also found that PO improves the level of WBC, MPO, and MDA, as well as thiol group formation and SOD and CAT activities, compared with the LPS group. The results of our investigation also show that PO significantly decreased the lung wet/dry ratio as an index of interstitial edema. Taken together, our findings reveal that PO extract dose-dependently displays anti-oxidant and anti-inflammatory activity against LPS-induced rat ALI, paving the way for rational use of PO as a protective agent against lung-related inflammatory disease.

Consumption of high-dose vitamin C (1250 mg per day) enhances functional and structural properties of serum lipoprotein to improve anti-oxidant, anti-atherosclerotic, and anti-aging effects via regulation of anti-inflammatory microRNA

2015 ◽  
Vol 6 (11) ◽  
pp. 3604-3612 ◽  
Author(s):  
Seong-Min Kim ◽  
So-Mang Lim ◽  
Jeong-Ah Yoo ◽  
Moon-Jea Woo ◽  
Kyung-Hyun Cho
Keyword(s):  
Vitamin C ◽  
Structural Properties ◽  
Serum Lipoprotein ◽  
High Dose ◽  
Aging Effects ◽  
Male Smoker ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
High Dose Vitamin

Increase of apoA-I in HDL and enhancement of anti-atherosclerotic activity by high-dose vitamin C consumption, especially in the male smoker group.

scholarly journals Identification of triptolide, a natural diterpenoid compound, as an inhibitor of lung inflammation

2010 ◽  
Vol 298 (6) ◽  
pp. L830-L836 ◽  
Author(s):  
Gary W. Hoyle ◽  
Christine I. Hoyle ◽  
Jing Chen ◽  
Weiyuan Chang ◽  
Ronald W. Williams ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Substance P ◽  
Lung Injury ◽  
Signaling Pathway ◽  
Lung Inflammation ◽  
A549 Cells ◽  
In Vivo Model ◽  
Anti Inflammatory ◽  
Stimulation Of

Inflammation is associated with various pulmonary diseases and contributes to the pathogenesis of acute lung injury. We previously identified a proinflammatory signaling pathway triggered by G protein-coupled receptors (GPCRs) in which stimulation of Gq-coupled GPCRs results in activation of the transcription factor NF-κB. Because damage to the lung causes the release of multiple mediators acting through Gq-coupled GPCRs, this signaling pathway is likely to contribute to inflammatory processes in the injured lung. In an effort to identify novel inhibitors of lung inflammation, the National Institutes of Health Clinical Collection, a library of 446 compounds, was screened for inhibitory activity toward production of IL-8 induced by stimulation of the Gq-coupled tachykinin 1 receptor with substance P in A549 cells. Twenty-eight compounds that significantly inhibited substance P-induced IL-8 production were identified. The most potent inhibitor was triptolide, a diterpenoid compound from Tripterygium wilfordii Hook F, a vine used in traditional Chinese medicine for the treatment of autoimmune diseases. Triptolide inhibited IL-8 production induced by substance P with an IC50 of 2.3 × 10−8 M and inhibited NF-κB activation in response to an agonist of the protease-activated receptor 2 with an IC50 of 1.4 × 10−8 M. Anti-inflammatory effects of triptolide were assessed in vivo using a chlorine gas lung injury model in mice. Triptolide inhibited neutrophilic inflammation and the production of KC (Cxcl1) in the lungs of chlorine-exposed mice. The results demonstrate that triptolide exhibits anti-inflammatory activity in cultured lung cells and in an in vivo model of acute lung injury.

Pharmacologically potentials of different substituted coumarin derivatives

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Benzene Ring ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Pharmacological Properties ◽  
Coumarin Derivatives ◽  
Wide Range ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Coumarin Compounds

Coumarin and its derivatives are widely spread in nature. Coumarin goes to agroup as benzopyrones, which consists of a benzene ring connected to a pyronemoiety. Coumarins displayed a broad range of pharmacologically useful profile.Coumarins are considered as a promising group of bioactive compounds thatexhibited a wide range of biological activities like anti-microbial, anti-viral,antiparasitic, anti-helmintic, analgesic, anti-inflammatory, anti-diabetic, anticancer,anti-oxidant, anti-proliferative, anti-convulsant, and antihypertensiveactivities etc. The coumarin compounds have immense interest due to theirdiverse pharmacological properties. In particular, these biological activities makecoumarin compounds more attractive and testing as novel therapeuticcompounds.

Sign in / Sign up

Export Citation Format

Share Document