Aflatoxin B1 contamination of feedstuff on a dairy farm in Northern Peru and aflatoxin M1 concentrations in raw milk

2021 ◽  
pp. 1-6
Author(s):  
I. Salazar ◽  
I. López ◽  
P. Glorio-Paulet ◽  
C. Gomez
Keyword(s):  
Soybean Meal ◽  
Dairy Farm ◽  
Maximum Level ◽  
Raw Milk ◽  
Aflatoxin Contamination ◽  
Aflatoxin M1 ◽  
Significant Difference ◽  
Northern Peru ◽  
Contamination Levels ◽  
Aflatoxin B

Research regarding aflatoxin contamination levels in Peru is limited, although aflatoxin M1 (AFM1) and aflatoxin B1 (AFB1) require surveillance because of their toxicity. European regulations state that the harmonised maximum level (ML) is 5 μg/kg for AFB1 in feedstuffs and 0.05 μg/kg for AFM1 in milk. Our study aimed to determine the annual variation levels of AFB1 in ingredients used in feedstuffs for dairy cows and those of AFM1 in milk at a typical intensive dairy farm in Northern Peru. For 1 year, milk (n=529) and feedstuff samples (n=235) were collected and aflatoxin levels were determined using a lateral flow immunoassay. We found that 16% of milk samples had AFM1 contamination above the ML. AFM1 level was significantly higher (P<0.05) in December (end of spring) than that in all other months. Throughout the year, the most used feedstuffs were maize, soybean meal and whole soybean. Among the maize samples (n=77), 2.59% had an AFB1 level above the ML, whereas 45% had an AFB1 level below the ML. On the other hand, neither the soybean meal (n=69) nor whole soybean samples (n=64) had an AFB1 level above the ML, 46.4 and 20%, respectively. In 50% (n=10) of cottonseed meal samples, AFB1 level was above the ML; in 20% of wheat middling samples, it was above the ML. Cottonseed and wheat middling samples were used for 2 and 5 months, respectively. AFB1 level in feedstuff showed a significant difference in December (P<0.05) compared with other months, specifically for maize and soybean meal. As the AFM1 level in milk results from AFB1 contaminated feedstuff, our results emphasise the need to implement specific quality measures to reduce contamination.

Collaborative Study of the Impedance Method for Examining Raw Milk Samples

1984 ◽  
Vol 47 (9) ◽  
pp. 707-712 ◽  
Author(s):  
R. FIRSTENBERG-EDEN
Keyword(s):  
Collaborative Study ◽  
Raw Milk ◽  
Plate Count ◽  
Impedance Method ◽  
Standard Plate Count ◽  
Milk Samples ◽  
Automated Method ◽  
Significant Difference ◽  
Standard Plate ◽  
Contamination Levels

The impedance method is a rapid automated method for determining bacteriological contamination levels. A collaborative study was done to establish the reproducibility of the impedance method in predicting counts of raw milk. Frozen and unfrozen raw milk samples, with counts in the range of 9 × 104 to 4 × 107 CFU/ml, were sent to six laboratories to be examined by the standard plate count method (SPC) and by the impedance method which produced Bactometer-predicted counts (BPC). The impedance results showed less variability than SPC among laboratories in all three trials. The variance between split samples was also smaller for the impedance method than for SPC. However, the variance between duplicate plates of the same sample was significantly smaller for SPC than for BPC. In one trial, the means of BPC and SPC were not significantly different, whereas in another trial there was a significant difference of ca. log10 0.27 between the means of the two methods. However, in this trial the extreme differences between laboratories counting the same sample were log10 0.42.

scholarly journals Iodine content in bulk tank milk samples in relation to dairy farm size

2014 ◽  
Vol 83 (10) ◽  
pp. S9-S13 ◽  
Author(s):  
Lenka Vorlová ◽  
Lucia Hodulová ◽  
Ivana Borkovcová ◽  
Hana Přidalová ◽  
Romana Kostrhounová ◽  
...  
Keyword(s):  
Dairy Farm ◽  
Iodine Content ◽  
Dairy Farms ◽  
Raw Milk ◽  
Farm Size ◽  
Milk Samples ◽  
Significant Difference ◽  
Organic Dairy ◽  
Bulk Tank ◽  
Organic Dairy Farms

The aim of this study was to compare the iodine content in raw milk from organic and conventional dairy farms of different sizes. Milk samples were collected between 2012 and 2013, and the iodine content was determined by a Sandell-Kolthoff reaction after dry alkaline digestion of the milk samples. Comparing the iodine content in raw milk samples from small sized dairy farms (116.76 ± 46.29 μg/l) and large sized dairy farms (173.70 ± 35.42 μg/l), a significant difference in iodine content was observed (P ≤ 0.05). The lowest values were found in small and medium dairy farms, 45.30 μg/l and 40.46 μg/l, respectively. High variability (112.92 ± 94.74 μg/l) in the iodine content was detected in raw milk from medium sized dairy farms. When considering milk samples from organic dairy farms (119.29 μg /l ± 40.37) vs. conventional dairy farms (136.55 μg/l ± 42.91), no significant difference was detected. These results indicate higher iodine content in milk from large dairy farms regardless of conventional or organic farming methods.

scholarly journals Occurrence and seasonal variation of aflatoxin M1 in raw cow milk collected from different regions of Algeria

2020 ◽  
Vol 13 (3) ◽  
pp. 433-439 ◽  
Author(s):  
Sarah Mohammedi-Ameur ◽  
Mohammedi Dahmane ◽  
Carlo Brera ◽  
Moustafa Kardjadj ◽  
Meriem Hind Ben-Mahdi
Keyword(s):  
Animal Feed ◽  
Control Program ◽  
Raw Milk ◽  
Aflatoxin Contamination ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Survey Period ◽  
Lactating Cows ◽  
The Usa

Background and Aim: Aflatoxins are metabolites of molds that exert potentially toxic effect on animals and humans. This study aimed to investigate the occurrence of aflatoxin M1 (AFM1) in raw cow milk collected during 1 year (2016-2017) from different regions of Algeria and risk factors associated with the contamination. Materials and Methods: During the survey period, 84 samples of raw milk were collected in three regions of Algeria (northeast, north center, and northwest) during four seasons. AFM1 levels were analyzed by competitive enzyme-linked immunosorbent assay. Results: AFM1 was detected in 39 (46.43%) samples (total mean concentration, 71.92 ng/L; range, 95.59-557.22 ng/L). However, the AFM1 levels exceeded the maximum tolerance limit set by the Food and Drug Administration in the USA (500 ng/L) in only 1 sample (1.19%). Statistical analysis revealed significant differences (p<0.005) between AFM1 levels in milk samples collected in the spring and autumn. The mean AFM1 levels in samples collected in the spring were significantly higher than those in samples collected in autumn. Conclusion: The survey indicates that farmers involved in milk production should be made aware of the adverse effects of aflatoxin contamination in animal feed. A systematic control program of supplementary feedstuff for lactating cows should be introduced by the public health authorities.

Number of export almond lots rejected in the EU due to USA sampling plans and aflatoxin contamination levels among lots tested

2010 ◽  
Vol 3 (2) ◽  
pp. 157-168 ◽  
Author(s):  
T. Whitaker ◽  
A. Slate ◽  
J. Adams ◽  
T. Birmingham
Keyword(s):  
Aflatoxin Contamination ◽  
Sampling Plan ◽  
Contamination Level ◽  
The European Union ◽  
Sampling Plans ◽  
Aflatoxin Concentration ◽  
The Usa ◽  
Contamination Levels ◽  
Aflatoxin B ◽  
The Eu

In March 2010, the European Union (EU) modified their aflatoxin limits and sampling plans for almonds, pistachios, and hazelnuts to closely resemble that developed by Codex in 2008. To assist the USA almond industry evaluate how to modify their aflatoxin sampling plan to minimise USA lots rejected by the EU at import, studies were conducted to: (a) develop a model to predict the number of USA almond lots rejected at origin and at destination by various Codex style aflatoxin-sampling plans used by the USA where accept/reject limits vary from EU limits of 8 ng/g aflatoxin B1 / 10 ng/g total aflatoxins (AFT) to 8, 6, 4, 2, and 0.5 ng/g AFT; (b) determine the effect of the level of aflatoxin contamination among lots tested in the USA on the number of lots accepted and rejected at origin and at destination; and (c) develop a method based upon lots rejected in the USA to predict the lots rejected several months later in the EU. For a given USA crop contamination level, as the accept/reject limits of the USA sampling plan decreases from 8 to 0.5 ng/g AFT, the number of lots rejected in the USA increases, the average aflatoxin concentration among all lots accepted for export decreases, the number of lots rejected in the EU decreases, and the average aflatoxin concentration among lots accepted in the EU decreases. For a given USA aflatoxin sampling plan used at origin, an increase in the USA crop aflatoxin contamination level increases the number of lots rejected at origin, increases the average aflatoxin concentration in USA lots accepted for export, increases the number of lots rejected in the EU, and increases the average aflatoxin concentration among lots accepted in the EU. Graphical techniques were developed to use the percentage of lots rejected in the USA to predict the percentage of USA lots rejected by the EU.

scholarly journals MC-Media Pad SA (Sanita-kun SA) for the Enumeration of Staphylococcus aureus in a Variety of Foods

2018 ◽  
Vol 101 (2) ◽  
pp. 456-467
Author(s):  
Hajime Teramura ◽  
Gail Betts ◽  
Yi Chen ◽  
Michael Brodsky ◽  
Yvonne Salfinger
Keyword(s):  
Staphylococcus Aureus ◽  
Incubation Temperature ◽  
Reference Method ◽  
Raw Milk ◽  
Sample Volume ◽  
Acceptance Criterion ◽  
Significant Difference ◽  
The Matrix ◽  
The Difference ◽  
Contamination Levels

Abstract MC-Media Pad SA (formerly known as Sanita-kun SA) is a dry rehydratable film medium for the enumeration of Staphylococcus aureus. The performance of the method in a variety of foods was compared with that of ISO 6888-1:1999, Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and Other Species) - Part 1: Technique Using Baird–Parker Agar Medium. The validated matrixes included pastrami, a sliced cooked chicken roll, cooked prawns, cold-smoked salmon, pasta salad, sandwich spread, fresh uncooked pasta, infant cereal, custard, and raw-milk Brie cheese. In the matrix study, five replicates at each of three contamination levels were tested as paired test portions. Across all matrixes, the difference in mean log10 values ranged from –0.32 to 0.10, which was within the acceptable range of –0.50 to 0.50. Thus, all 10 matrixes met the acceptance criterion at all concentration levels. Further, only two matrixes, cooked prawns and raw-milk Brie cheese, had 95% confidence limits outside the –0.50 to 0.50 criterion, and these were at the lowest concentration level for each matrix. The candidate method sr varied from 0.03 to 0.22 log10 CFU/g. This compares favorably with the reference method SD, which ranged from 0.06 to 0.30 log10 CFU/g. The candidate and reference methods detected 51 of 53 inclusivity strains, with both methods not detecting the same two strains. The candidate method did not detect any of the 32 exclusivity strains, whereas the reference method did not detect 30 of the 32 exclusivity strains; the 2 strains detected by the reference method were S. delphini and S. hyicus, both developing atypical colonies on Baird–Parker plates. The product consistency study demonstrated no significant difference between lots of product and supported the 1 year shelf life. Robustness testing yielded no significant differences when small variations were made in sample volume, incubation temperature, and incubation time. Thus, the data show equivalent or better performance of the Sanita-kun SA/MC-Media Pad SA method compared with the International Organization for Standardization reference method, in support of AOAC Performance Tested MethodSM certification.

Aflatoxin M1 contamination level in Iranian milk and dairy products: a systematic review and meta-analysis

2020 ◽  
Vol 13 (1) ◽  
pp. 67-82 ◽  
Author(s):  
S. Hamzeh Pour ◽  
S. Mahmoudi ◽  
S. Masoumi ◽  
S. Rezaie ◽  
A. Barac ◽  
...  
Keyword(s):  
Systematic Review ◽  
Dairy Products ◽  
Meta Analysis ◽  
Raw Milk ◽  
Aflatoxin M1 ◽  
Contamination Level ◽  
Livestock Feeds ◽  
Aflatoxin B ◽  
Mean Concentration ◽  
Milk And Dairy Products

Aflatoxin M1 is a derivate of aflatoxin B1 and an important contaminant of milk and dairy products. This systematic review and meta-analysis was conducted on relevant Persian and English original articles in national and international databases with no time limits until 1 January 2018. In total 605 articles were found among which 70 articles met the inclusion criteria for meta-analysis. The prevalence (95% confidence interval (CI)) and mean concentration (95% CI) of aflatoxin M1 was found to be 64% (53-75%) and 39.7 ng/l (31.9-47.4 ng/l) in raw milk, 95% (89-98%) and 62.3 ng/l (40.6-84 ng/l) in pasteurised milk, 71% (56-84%) and 60.1 ng/l (30.9-89.3 ng/l) in sterilised milk, 59% (20-93%) and 5.5 ng/l (3.3-7.7 ng/l) in breast milk and 72% (61-81%) and 82.3 ng/kg (63.7-100.9 ng/kg) in dairy products. In general, 9% (4-16%) of milks and 10% (4-17%) of dairy products had aflatoxin M1 in concentrations exceeding the permitted level of Iranian standards (500 ng/l). Based on the maximum permitted aflatoxin M1 concentration in standards of Europe (50 ng/l), these percentages increase to 25% (18-32%) for milks and 18% (9-29%) for dairy products. According to the results, further control and preventive measures should be applied on livestock feeds because decreased aflatoxin B1 contamination at this level results in decreased aflatoxin M1 in milk and dairy products.

scholarly journals Surveillance of Aflatoxin and Microbiota Related to Brewer's Grain Destined for Swine Feed in Argentina

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Gisela A. Gerbaldo ◽  
Carina M. Pereyra ◽  
Lilia R. Cavaglieri ◽  
Francisco Ruiz ◽  
Liliana Pascual ◽  
...  
Keyword(s):  
Storage Conditions ◽  
Fungal Species ◽  
Aflatoxin Contamination ◽  
Swine Production ◽  
Natural Contamination ◽  
Contamination Levels ◽  
Aflatoxin B ◽  
Prevalent Species ◽  
Regular Practice

Córdoba province in the center of Argentina is an important area of swine production. The use of industry by-product (brewer's grain) as feedstuff for swine is a regular practice and increases animal performance on these animals production. The occurrence of aflatoxin contamination is global, causing severe problems especially in developing countries. No reports on aflatoxin B1production, micoflora, and potential aflatoxin B1producing microorganism from brewer's grain are available. The aims of this study were (1) to isolate the microbiota species from brewer's grain, (2) to determine aflatoxin B1natural contamination levels, and (3) to determine the ability ofAspergillussectionFlaviisolates to produce aflatoxinsin vitro. Physical properties, total fungal counts, lactic acid bacteria, and fungal genera distribution were determined on this substrate. In 65% of the samples, fungal counts were higher than recommended by GMP, and lactic bacterium counts ranged from1.9×105to4.4×109 CFUg−1.Aspergillusspp. prevailed over other fungal genera.Aspergillus flavuswas the prevalent species followed byA. fumigatus. Aflatoxin B1levels in the samples were higher than the recommended limits (20 ng g−1) for complementary feedstuffs. SeveralAspergillussectionFlavistrains were able to produce aflatoxin B1  in vitro. Inadequate storage conditions promote the proliferation of mycotoxin-producing fungal species. Regular monitoring of feeds is required in order to prevent chronic and acute toxic syndromes related to this kind of contamination.

Carry-over of aflatoxin from cows' food to milk

1992 ◽  
Vol 55 (2) ◽  
pp. 163-168 ◽  
Author(s):  
A. Veldman ◽  
J. A. C. Meijs ◽  
G. J. Borggreve ◽  
J. J. Heeres-van der Tol
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Daily Intake ◽  
Maximum Level ◽  
Experimental Period ◽  
Raw Milk ◽  
Lactation Period ◽  
Dairy Foods ◽  
Carry Over ◽  
Aflatoxin B

AbstractTwo experiments were made. In experiment 1, dairy cows in early lactation (2 to 4 weeks) and dairy cows in late lactation (34 to 36 weeks) were given aflatoxin B, (AFBt)at a level of 34 to 39 fig/day by feeding them contaminated compound food. The aflatoxin Mrcontent(AFMj) of raw milk was determined four times during the experimental period of 14 days. The milk yield was measured daily.The carry-over rate of aflatoxin was proportionately 0·062 and 0·018 for cows in early and late lactation respectively. This difference was not only due to milk production level but possibly also associated to AFB, liver metabolism.In experiment 2, eight high (40 kg milk per day) and eight low (16 kg milk per day) milk yielding cows, carry-over of AFB1 from compound food into AFM, in milk was measured at different levels ofAFB7 intake ranging from 7 to 57 fig/day. Independent of AFBj-intake, high-producing dairy cows had a higher carry-over rate than low producing animals (proportionately 0·038 v. 0·025). Carry-over ofAFB1 to AFM, was linearly correlated with milk yield. Variations in the level of AFM1 in milk of individual cows were not due to variations in milk yield. The relationship between AFB2 intake per day and AFM^content in milk per kg could be described by the formula: AFMj (ng/kg milk) = 119 AFB, intake (fig per cow per day) + 1·9. This means that in order to produce milk with less than 0·05 fig AFM, per kg milk the average daily individual intake in a herd should be limited to 40 fig AFB7 per day.As dairy cows in their early to mid lactation period consume large quantities of compound food, a maximum level of AFB3 in dairy foods has to be set at an acceptable daily intake (ADI) value to guarantee that AFM1 levels in milk do not exceed tolerable levels.

scholarly journals Occurrence and Exposure Assessment of Aflatoxin B1 in Omena (Rastrineobola argentea) from Kenya

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Esther Marijani ◽  
Harrison Charo-Karisa ◽  
Emmanuel Kigadye ◽  
Sheila Okoth
Keyword(s):  
Health Risk ◽  
Maximum Level ◽  
Exposure Dose ◽  
Aflatoxin Contamination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Consumption ◽  
Fish Feed ◽  
Potential Health ◽  
Feed Production ◽  
Aflatoxin B

Omena (Rastrineobola argentea) is the most consumed fish species in Kenya. In this study, we assessed the occurrence of aflatoxin B1 (AFB1) in Omena and the potential health risk of AFB1 to Kenyan consumers of this fish. A total of 74 samples comprising Omena intended for human consumption and fish feed production were analyzed in this study. Aflatoxin levels in Omena were determined using the enzyme-linked immunosorbent assay (ELISA). Omena intended for fish feed production was most contaminated with a mean concentration of 46.93 μg·kg−1 (2.24–115.23 μg·kg−1) compared with Omena intended for human consumption (mean = 19.42 μg·kg−1, range = 2.01–49.30 μg·kg−1). Thirty-five positive samples (83.3%) exceeded the maximum level permitted (5 μg·kg−1) by the East Africa Community standard for food used for human consumption. The exposure dose of AFB1 from consuming Omena was estimated to be 1.34 ng·kg−1 BW day−1, and margin of exposure (MoE) value for AFB1 was found to be 126.3, which indicates health risk to Omena consumers. The results suggest that the current situation of aflatoxin contamination in Omena has an adverse effect on the health of the consumers as well as the animals. Therefore, more surveys are needed to understand the scope and extent of aflatoxin contamination in Omena.

Risk associated with the intake of aflatoxin M1 from milk in Iran

2019 ◽  
Vol 12 (2) ◽  
pp. 191-200 ◽  
Author(s):  
A. Fooladi Moghaddam ◽  
M. Rychlik ◽  
H. Hosseini ◽  
B. Janat ◽  
H. Yazdanpanah ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Maximum Level ◽  
Iranian Population ◽  
Aflatoxin M1 ◽  
International Agency ◽  
Management Processes ◽  
Heat Treated ◽  
Current Maximum ◽  
Local Exposure ◽  
Aflatoxin B

Aflatoxin M1 is an oxidative metabolite of aflatoxin B1 formed in liver and excreted into milk, urine and faeces of dairy cattle and other mammalian species. The International Agency for Research on Cancer classified aflatoxin M1 in Group 2B because of its potential to get bioactivated to a mutagen analogous to aflatoxin B1. Risk assessments are undertaken to guide food regulators and scientists in risk management processes, such as the legislative levels or guideline targets for mycotoxins in food supplies. Using existing international resources for hazard data and local exposure data, and based on cancer potency as the endpoint, the risk of exposure to aflatoxin M1 in milk for the Iranian population was calculated considering various scenarios. During 2014-2015, 518 samples were collected from the market and tested for aflatoxin M1 contamination by HPLC-FLD. The most probable scenarios calculated as mean occurrence multiplied by the mean consumption in consumers of milk, and for maximum level allowed for aflatoxin M1 in milk with 99 percentiles of milk consumption showed the risk of 0.08 and 0.72 additional liver cancer cases per year for the Iranian population, respectively. Thus, our study reveals a low risk and that the current maximum limit of 100 ng/l for aflatoxin M1 in milk, heat treated milk and flavoured milk is sustainable.

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