scholarly journals Effects of Methanol Extract of Morus alba on Hematological Parameters and Liver Histology of African Catfish (Clarias gariepinus)

2012 ◽  
Vol 11 (17) ◽  
pp. 3202-3205
Author(s):  
H.M. Daud ◽  
A. Sheikhlar ◽  
A.R. Alimon ◽  
C.R. Saad
2018 ◽  
Vol 14 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Nur Hidayahanum Hamid ◽  
Hassan Mohd Daud ◽  
Prapansak Srisapoome ◽  
Hasliza Abu Hassim ◽  
Md Sabri Mohd Yusoff ◽  
...  

Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African catfish, Clarias gariepinus during pre- and post-challenge with aquatic pathogen, Aeromonas hydrophila. The probiotics were previously isolated from vegetable wastes (mung bean sprouts, Vigna radiate and cucumber, Cucumis sativus) which have been fermented for 7 days. The experimental fish (270 tails) with an average weight of 5.13 ± 1.03 g were distributed and divided randomly  into  i) control (30 tails), fed with commercial diet ii) E1 (30 tails), fed with  diets  supplemented  with  108 CFU/ml  of  E. faecium isolated from fermented cucumber, iii) E2 (30 tails), fed with 108 CFU/ml  of  E. faecium isolated from fermented mung bean sprouts. The feeding trial was conducted for 50 days. All experimental groups were then challenged with A. hydrophila (1.5 × 106 CFU/mL) via intraperitoneal injection on day 51. Prior to challenge, blood samples were collected from five fish randomly selected from each group on the day 51 (pre-challenge).  After 72 hours of post-challenge, blood samples were again collected from five fish from each groups. The hematological parameters such as total erythrocyte count  (RBC),  total  leucocyte  count  (WBC),  packed cell volume (PCV),  hemoglobin  (Hb), the derived blood indices of mean corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) were examined.  Hematological profiles of pre- and post-challenge infected juvenile catfish were compared with the control groups. The RBC, Hb, WBC, PCV, MCV and MCHC of fish fed with probiotics showed higher significant difference (P<0.05) as compared to control groups during pre- and post-challenge of pathogen. The high level of RBC and WBC during pre- and post-challenge showed the capability of the probiotics to improve the immune response of juvenile African catfish and thus increased the fish disease resistance against A. hydrophila infection. The result suggested that E. faecium could be used effectively as a probiotic in aquaculture.


2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Atefeh Sheikhlar ◽  
Abd Razk Alimon ◽  
Hassan Daud ◽  
Chee R. Saad ◽  
Carl D. Webster ◽  
...  

Two experiments were simultaneously conducted withMorus alba(white mulberry) foliage extract (MFE) as a growth promoter and treatment ofAeromonas hydrophilainfection in separate 60 and 30 days trail (Experiments 1 and 2, resp.) in African catfish (Clarias gariepinus). In Experiment 1, four diets, control and control supplemented with 2, 5, or 7 g MFE/kg dry matter (DM) of diet, were used. In Experiment 2, fish were intraperitoneally infected withAeromonas hydrophilaand fed the same diets as experiment 1 plus additional two diets with or without antibiotic. Results of experiment 1 showed that growth was unaffected by dietary levels of MFE. Treatments with the inclusion of MFE at the levels of 5 and 7 g/Kg DM had no mortality. Red blood cells (RBC), albumin, and total protein were all higher for the treatments fed MFE (5 and 7 g/Kg DM). Results of experiment 2 showed RBC, hemoglobin, hematocrit, globulin, albumin, and total protein improved with the increase in MFE in the infected fish. The dietary MFE at the level of 7 g/kg DM reduced mortality rate. In conclusion, MFE at the level of 7 g/kg DM could be a valuable dietary supplement to cure the infected fish.


2021 ◽  
Vol 37 (2) ◽  
pp. 117-126
Author(s):  
Abdel-Hay Abdel-Hay ◽  
Monira Elsawy ◽  
Wasseem Emam ◽  
Wael Eltras ◽  
Radi Mohamed

This study contributes data on haematological and biochemical parameters of African catfish, Clarias gariepinus. It employed a 3 ? 2 factorial design with three ponds of different water depth (0.5, 1 and 1.5 m) and two types of feed (floating and sinking). Twelve earthen ponds (1 m x 2 m) were stocked with 16 fingerlings catfish each (mean weight ~100g) and their blood parameters were monitored over 12 weeks. Differences in hematological parameters related to water depth were mostly significant, and better results were recorded in fish reared in shallower water ponds. Feed type showed improved hematological parameters with using of sinking diet. Most biochemical parameters showed significant differences in pond waters depth and feed type with better results coincided with rearing fish in shallower water depth and with sinking feed. Conclusively, culturing Catfish in shallow ponds (0.5 m) and use of sinking feed improve physiological response and health condition.


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