scholarly journals Toxicological evaluation of methanol extract of Khaya senegalensis leaves in African catfish Clarias gariepinus

2015 ◽  
Vol 19 (3) ◽  
pp. 557
Author(s):  
OA Abui ◽  
MM Motouke
2015 ◽  
Vol 2 (3) ◽  
pp. 475-482
Author(s):  
Matouke Matouke Moise ◽  
Obadiah Audu Abui

Haematological and histopatholoical effects of methanolic extract of K. senegalensis leaves was investigated on Clarias gariepinus over a period of 96h exposure. The median lethal concentration of the extract was 199.69mg/L. The extract caused decreased in total erythrocytes (TEC) and Packed Cell volume (PCV) respectively and increased of total leukocytes (TLC). Histopathological lesions in the liver, cytoplasmic degeneration, less intracellular space, mild necrosis, sinusoidal blood congestion and marked blood congestion in hepathocytes were recorded. However the severity but not the type of lesions was concentration-dependent. Though, the degree of tissue change (DTC) varied with the methonolic extract used. There was significant association (P<0.05) between the DTC and K. senegalensis methanolic concentration. The cumulative DTC indicated a moderate damage in the liver. The extract was considered toxic to the exposed fish and therefore deleterious on the organs of C. gariepinus.Res. Agric., Livest. Fish.2(3): 475-482, December 2015


Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.


2010 ◽  
Vol 9 (1) ◽  
Author(s):  
Lutfi Lutfi

<p><em>Effect of four types of diluents</em><em>s</em><em> and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. </em><em>The steps in preparation the 16 treatments combination of the diluents</em><em> </em><em>are </em><em>preparation of diluents, mixing </em><em>diluents </em><em>with DMSO, packing </em><em>of semen </em><em>in</em><em>to</em><em> 0.3-ml straw, equilibration of </em><em>semen </em><em>at 4 °C for 30 minutes, freezing </em><em>of </em><em>semen in nitrogen vapor liquid </em><em>at </em><em>a height of 6.5 cm for 10 minutes</em><em>,</em><em> and subsequent</em><em>ly</em><em> storage of </em><em>semen </em><em>in liquid nitrogen (-196</em><em> <sup>0</sup></em><em>C) for further analy</em><em>sis</em><em> </em><em>of </em><em>post-thawing motility (PTM). </em><em>The result showed that t</em><em>he highest level of motility of spermatozoa </em><em>was </em><em>in </em><em>treatment </em><em>P</em><em><sub>1</sub></em><em>D</em><em><sub>15 </sub></em><em>(45.7 ± 4.3%) and the lowest </em><em>was </em><em>in </em><em>treatment </em><em>P<sub>2</sub>D<sub>20</sub> (14.5 ± 13.2%). The best diluent </em><em>in </em><em>this observation </em><em>was </em><em>diluents containing NaCl, KCl, CaCl<sub>2</sub> and NaHCO<sub>3</sub>. The best concentration </em><em>was </em><em>DMSO 15%. While the best interaction between the concentration of DMSO diluents is P<sub>1</sub>D<sub>15</sub> treatments containing NaCl, KCl, CaCl <sub>2</sub> and NaHCO<sub>3</sub> with a combination of 15% DMSO concentration. </em><em>The </em><em>conclusion </em><em>of the research is that </em><em>diluents containing NaCl, KCl, CaCl<sub>2</sub> and NaHCO<sub>3</sub> with a combination of 15% DMSO concentration</em><em> can be used in </em><em>cryopreservation of African catfish semen</em><em>.</em></p>


2012 ◽  
Vol 16 (2) ◽  
pp. 83-98 ◽  
Author(s):  
Shadia Kadry ◽  
Afnan Amer ◽  
Mohamed Marzouk ◽  
Magdy Hanna ◽  
Amal Azmy ◽  
...  

2021 ◽  
Vol 93 ◽  
pp. 102147
Author(s):  
Erkan Gümüş ◽  
Adil Yılayaz ◽  
Mahir Kanyılmaz ◽  
Bahar Gümüş ◽  
Murat Balaban

2011 ◽  
Vol 19 (6) ◽  
pp. 1025-1034 ◽  
Author(s):  
Ali Karami ◽  
Annie Christianus ◽  
Hadi Zokaeifar ◽  
Khairul Zamri Saad ◽  
Fahmi T. J. Imraan ◽  
...  

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