In vitro Effect of Terminalia arjuna Bark Extract on Antioxidant Enzyme Catalase

2007 ◽  
Vol 2 (8) ◽  
pp. 698-708 ◽  
Author(s):  
T.N. Padma Sree ◽  
S. Krishna Kumar . ◽  
A. Senthilkumar . ◽  
Gopala Krishna Aradh . ◽  
Sathyanarayana N. Gu .
Keyword(s):  
Antioxidant Enzyme ◽  
Bark Extract ◽  
Terminalia Arjuna ◽  
Vitro Effect ◽  
Antioxidant Enzyme Catalase

scholarly journals INHIBITORY EFFECT OF A STANDARDIZED HYDROETHANOLIC EXTRACT OF TERMINALIA ARJUNA BARK ON ALPHA-AMYLASE ENZYMEINHIBITORY EFFECT OF A STANDARDIZED HYDROETHANOLIC EXTRACT OF TERMINALIA ARJUNA BARK ON ALPHA-AMYLASE ENZYME

2018 ◽  
Vol 11 (4) ◽  
pp. 366 ◽  
Author(s):  
Sushant A Shengule ◽  
Sanjay Mishra ◽  
Shweta Bodhale
Keyword(s):  
Inhibitory Activity ◽  
High Performance ◽  
Soxhlet Extraction ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Terminalia Arjuna ◽  
Hydroethanolic Extract

 Objective: The present study was initiated to screen the hydroethanolic bark extract for α-amylase inhibitory activity and standardization of the Terminalia arjuna for polyphenolic phytochemicals using high-performance liquid chromatography-photo diode array (HPLC-PDA) method.Methods: The T. arjuna bark sample was extracted with ethanol: water (70:30 v/v) using Soxhlet extraction. A Dionex P680 HPLC system was used to acquire chromatograms. The screening of extract of T. arjuna bark has performed for in vitro α-amylase inhibitory assay. Each experiment was repeated 3 times. All values were expressed mean ± standard deviation.Results: The content of arjunetin, arjungenin, gallic acid, ellagic acid, and quercetin was 0.47, 8.22, 2.443, 7.901, and 3.20 mg/g, respectively, in a hydroethanolic extract of T. arjuna. The hydroethanolic extract of T. arjuna bark and acarbose has shown an inhibitory activity with an IC50 value 145.90 and 62.35 μg/mL, respectively.Conclusion: The hydroethanolic extract T. arjuna bark demonstrates α-amylase inhibitory activity due to a synergistic effect of the phytochemical constituents present in it. This study suggests that one of the mechanisms of this plant for antidiabetic activity is through the inhibition of α-amylase enzyme.

scholarly journals TERMINALIA ARJUNA TRANSDERMAL MATRIX FORMULATION CONTAINING DIFFERENT POLYMER COMPONENTS

2019 ◽  
pp. 266-270 ◽  
Author(s):  
DINANATH GAIKWAD ◽  
NAMDEO JADHAV
Keyword(s):  
Ex Vivo ◽  
Skin Irritation ◽  
Research Work ◽  
Bark Extract ◽  
Terminalia Arjuna ◽  
Diffusion Study ◽  
Matrix Formulation ◽  
Transdermal Patches ◽  
In Vitro Diffusion

Objective: The objective of this research work was to prepare a transdermal matrix formulation containing different polymer components for topical delivery. Methods: Terminalia arjuna bark extract loaded transdermal patches were prepared using solvent casting technique with different amount of chitosan and Eudragit RL 100 batches were prepared according to 32 factorial designs. Results: The transdermal patches prepared were evaluated for different physicochemical properties, determination of drug content, in vitro diffusion study, ex vivo study, skin irritation study, and stability study. Infrared studies indicate the absence of chemical interaction or any changes in the chemical composition of extract during the preparation of transdermal patch. In vitro diffusion study and ex vivo diffusion study of optimized batch S3 showed drug releases to 74.56–69.12%, respectively, up to 12 h. Skin irritation study indicates that the extract and excipients used in the patch do not show any irritating effect on the skin. All the prepared transdermal matrix formulations were found to be stable on storage. Conclusion: It can be concluded that prepared matrix formulation containing different polymer components can be used for transdermal delivery for the treatment of chronic ailments such as cardiovascular disorder.

scholarly journals In vitro effect of pine bark extract added to fruit juices on gutinflammation after gastrointestinal digestion

2013 ◽  
Vol 72 (OCE1) ◽  
Author(s):  
C. Frontela-Saseta ◽  
R. López-Nicolás ◽  
C. A. González-Bermúdez ◽  
P. Peso-Echarri ◽  
L. Fernández-Palacios ◽  
...  
Keyword(s):  
Fruit Juices ◽  
Pine Bark ◽  
Bark Extract ◽  
Gastrointestinal Digestion ◽  
Vitro Effect

scholarly journals HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC STEM BARK EXTRACT OF KNEMA ATTENUATA (HOO K. F. AND THOMSON) WARB

2020 ◽  
pp. 78-82
Author(s):  
SUPRIYA RAJA H.
Keyword(s):  
Cell Line ◽  
Antioxidant Defense ◽  
Serum Levels ◽  
Stem Bark ◽  
Hepatoprotective Activity ◽  
Bark Extract ◽  
Control Group ◽  
Stem Bark Extract ◽  
Vitro Effect

Objective: To investigate the hepatoprotective activity of ethanolic stem bark extract (ESBE) of Knema attenuata against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar rats using both in vivo and in vitro models. Methods: Animals were treated orally with ESBE (250 mg kg-1 and 500 mg kg-1) once daily for 6 d and CCl4 on the 4th d. On the 7th d, animals were sacrificed and the blood samples were collected to measure the serum levels of biochemical parameters, whereas the liver homogenates were utilized for estimating the antioxidant defense. The hepatoprotective efficacy of the extract was further ensured in vitro using human liver hepatocellular carcinoma (HepG2) cell line against CCl4 induced toxicity. The cell line viability was determined using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: ESBE effectively reduced (p<0.001) the elevated serum levels of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and Alkaline phosphatase (ALP) when compared to the toxicant control group. ESBE 500 mg kg-1significantly raised the antioxidant defense (p<0.0001) by reducing the malondialdehyde (MDA) level and enhancing hepatic reduced glutathione (GSH) level in comparison to the CCl4 control group. The in vitro effect was investigated using CCl4 exposed HepG2 cells. Pretreatment with ESBE showed a dose-dependent increase in percentage cell viability ranged between 44 to 57% at 12.5-100 μg ml-1concentrations (p<0.001, when compared to the control cells).  Conclusion: Present study confirms the hepatoprotective activity of the stem bark extract of K. attenuata against CCl4‑induced liver damage.

scholarly journals Nanosuspension enhances dissolution rate and oral bioavailability of Terminalia arjuna bark extract in vivo and in vitro

2020 ◽  
Vol 10 (4) ◽  
pp. 164
Author(s):  
Fatiqa Zafar ◽  
Nazish Jahan ◽  
Khalil-Ur-Rahman ◽  
MuhammadRafique Asi ◽  
Waseeq-Ul-Islam Zafar
Keyword(s):  
Dissolution Rate ◽  
Oral Bioavailability ◽  
Bark Extract ◽  
Terminalia Arjuna

Terminalia arjuna induced testicular assault through Leydig cell derangement: an in vitro approach

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Arnab Banerjee ◽  
Bithin Kumar Maji ◽  
Alok Chattopadhyay
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Inflammatory Responses ◽  
Bark Extract ◽  
Terminalia Arjuna ◽  
Dependent Manner ◽  
Duration Of Treatment ◽  
Medicinal Uses ◽  
Dose Dependent

Abstract Objectives Terminalia arjuna (TA) has been widely used as folk medicine since ancient times. Apart from its therapeutic properties it also has anti-spermatogenic activity, but its effectiveness and mode of action on male gonadal activity remains to be revealed. Methods TA bark extract was dissolved in 1% dimethyl sulfoxide and applied in primary Leydig cell culture from rat testis in dose dependent manner. After 24 h of treatment cellular toxicity marker, cytokines, steroid 5-alpha-reductase 1 (SRD5A1) and androgen receptor (AR) were measured. Results Present study first proposed the cytotoxic impacts of TA on Leydig cells via leakage of lactate dehydrogenase and inflammatory responses by altering the pro- and anti-inflammatory cytokines in dose-dependent-manner. Furthermore, down regulation of SRD5A1 and AR indicated inhibition of normal steroidogenesis which well corroborated with cytotoxity and inflammation related parameters to promote damage of Leydig cell function. Nevertheless, it can be speculated that the inhibition of normal steroidogenesis and thereby spermatogenesis induced by TA is possibly due to the presence of β-sitosterol. Conclusions Therefore, the dose and duration of treatment should be carefully monitored in cases of its medicinal uses and longer duration should be avoided to protect reproductive health and fertility.

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