Effects of Biofloc Application on Survival Rate, Growth Performance and Specific Growth Rate of Pacific Whiteleg Shrimp, Penaeus vannamei Culture in Closed Hatchery System

Fulfill needs of Siamese gourami still rely on the catch from nature, so the population in nature has become decreased. The increase of Siamese gourami fish production from culture is still constrained by the survival rate and slow growth rate in the seed phase. The purpose of this research was to determine the effect of Siamese gourami stocking density on the application of optimum temperature and salinity to growth performance for optimization of production capacity. Application of optimum temperature and salinity refers to the results of previous research, i.e., temperature of 28 °C and salinity 3 ‰. Siamese gourami seed with length of 2.96 ± 0.42 cm and weight of 5.27 ± 0.10 g used in the research. The experimental design used completely randomized design with four treatments, i.e. 1 fish L-1, 2 fish L-1, 3 fish L-1 and 4 fish L-1. The results showed that there were an effect of different stocking density on absolute growth weight and length, survival rate, specific growth rate and productivity. This is shown in the C treatment which growth was 4.95 ± 0.037 g, length 3.51 ± 0.01 cm, survival rate 93.47%, specific growth rate 1.22% and fish productivity of 625.76 g.

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Efek nisbah kelamin jantan dan betina yang berbeda terhadap kinerja pertumbuhan yuwana ikan nila biru, Oreochromis aureus (Steindachner1864) [Effect of different male and female sex ratio on growth of juvenile blue tilapia Oreochromis aureus (Steindachner 1864)]

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v17i1.304 ◽

2017 ◽

Vol 17 (1) ◽

pp. 55

Author(s):

Adam Robisalmi ◽

Priadi Setyawan ◽

Bambang Gunadi

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Growth Performance ◽

Specific Growth ◽

Feed Conversion ◽

Oreochromis Aureus ◽

Male And Female ◽

C 50 ◽

Blue Tilapia

Blue tilapia is one strain of tilapia potential to be developed. This fish has highly tolerant of low temperatures and salinity. In the development of aquaculture of tilapia, male monosex give higher production than mix male-female culture. This activity aims to determine the growth performance of juvenile blue tilapia reared by different ratio of male and female. The study was conducted for 90 days at the Research Institute for Fish Breeding Sukamandi. The fish used are blue tilapia stocking with the initial weight 32.32±2.34 g. Rearing juvenile of blue tilapia using net 2x1 m2 with stocking density 30 fish per net. The treatment is a difference in the ratio of male and female i.e A (100% male), B (75% male: 25% female), C (50% male: 50% female), D (25% male: 75% female), and E (100% female). The fish were fed with formulated food (32% protein) twice daily as much as 5 % of the biomass. The parameters observed were absolute growth, specific growth rate, daily growth rate, feed conversion ratio and survival rate. The results showed A, blue tilapia with 100% male had highest growth performance, longest and the highest weight i.e 8.33±0.67cm and 136.50± 11.92g; with highest specific growth rate, food conversion and survival rate, i,e 1.76±0.05 weight day-1, 1.52±0,20 and 96.66%. While the lowest growth and feed conversion was D, fish mix culture of blue tilapia (25% male: 75% female). Blue tilapia male 100% monosex is recommended to use in aquaculture because it showed the highest growth with lower feed conversion. AbstrakIkan nila biru (Oreochromis aureus) merupakan satu jenis ikan yang potensial untuk dikembangkan. Ikan ini memiliki keunggulan toleran terhadap suhu rendah dan salinitas tinggi. Dalam perkembangan budi daya, populasi ikan nila tung-gal kelamin jantan diyakini memberikan hasil produksi lebih baik dibandingkan kelamin campuran. Kegiatan ini bertu-juan untuk mengevaluasi performa pertumbuhan yuwana ikan nila biru yang dipelihara dengan nisbah kelamin jantan dan betina berbeda. Penelitian dilakukan di Balai Penelitian Pemuliaan Ikan Sukamandi selama 90 hari. Ikan yang di-gunakan adalah ikan nila biru dengan bobot awal tebar 32,32±2,34 g. Yuwana nila biru dipelihara di hapa berukuran 2x1 m2 dengan padat tebar 30 ekor per hapa. Perlakuan adalah perbedaan nisbah kelamin jantan dan betina yaitu A (100% jantan), B (75% jantan : 25% betina), C (50% jantan : 50% betina), D (25% jantan : 75% betina) dan E (100% betina). Selama pemeliharaan ikan diberi pakan berkadar protein 32% dengan frekuensi dua kali sehari sebanyak 5% dari bobot ikan. Parameter yang diamati meliputi pertumbuhan mutlak, laju pertumbuhan spesifik, laju pertumbuhan harian, nisbah konversi pakan, dan sintasan. Hasil penelitian menunjukkan populasi ikan nila biru yang dipelihara 100% jantan mempunyai performa pertumbuhan panjang dan bobot tertinggi sebesar 8,33±0,67cm dan 136,50±11,92 g dengan laju pertumbuhan spesifik yang tinggi sebesar 1,76±0,05% bobot hari-1, konversi pakan 1,52±0,20 dan sintasan 96,66%, sedangkan pertumbuhan terendah pada populasi ikan nila biru campuran (25% jantan : 75% betina). Pengguna-an ikan nila biru monoseks jantan 100% dianjurkan untuk digunakan dalam kegiatan budi daya karena mempunyai pertumbuhan tertinggi dengan konversi pakan yang rendah.

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Growth performance of bonylip barb Osteochilus vittatus (Valenciennes, 1842) fed on combination of Lemna (Lemna perpussila Torr) and commercial diet

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v21i2.576 ◽

2021 ◽

Vol 21 (2) ◽

pp. 151-165

Author(s):

Djamhuriyah S Said ◽

Novi Mayasari ◽

Dwi Febrianti ◽

Tjandra Chrismadha

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Growth Performance ◽

Specific Growth ◽

Commercial Diet ◽

Alternative Feed ◽

Commercial Feed ◽

Absolute Growth ◽

C 50

Bonylip barb (Osteochilus vittatus Valenciennes, 1842) is a native fish of Indonesia's inland waters which has become a cultivated fish commodity. However, production tends to decrease due to high feed costs. The alternative feed types need to be found to anticipate the problem. Lemna perpusilla Torr is one of macrophytes plant which potentially used as an alternative feed due to high protein content. This study aims to evaluate the growth performance of fish fed with a combination of Lemna and commercial feed. As much of each 14 fishes were rearing in a 45x45x40 cm3 aquarium and fed for 84 days or 12 weeks. There are 5 combinations of feed, i.e feed A (100% commercial feed), B (75% commercial feed + 25% Lemna), C (50% commercial feed + 50% Lemna), D (25% commercial feed + 75% Lemna), and E (100% Lemna). The fishes were fed by commercial feed as much as 3% of the total fish, while administering Lemna at 30% of body weight. Growth performances were measured i.e standard and total length, absolute growth, specific growth rate and survival rate. The results showed that fish was able to growth using combination of commercial feed and Lemna. Data analysis was conducted descriptively that showed that fish fed by feed C (50% commercial feed + 50% Lemna) produced the highest length and weight, absolute growth and specific growth rate compared to other treatments. The highest survival rate was at fish fed by feed E (100% Lemna). The use of Lemna as an alternative feed is expected to reduce dependence on commercial feed.

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Growth Performance of Hypophthalmichthys molitrix and Barbodes gonionotus Fingerlings by Feeding Microalgae Cultured on Fertilizer Factory Effluent

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v45i4.7328 ◽

1970 ◽

Vol 45 (4) ◽

pp. 315-322

Author(s):

MA Toyub ◽

MI Miah ◽

MAB Habib

Keyword(s):

Growth Rate ◽

Weight Gain ◽

Survival Rate ◽

Specific Growth Rate ◽

Growth Performance ◽

Specific Growth ◽

Silver Carp ◽

Scenedesmus Obliquus ◽

Hypophthalmichthys Molitrix ◽

Control Survival

Growth performance of Hypophthalmichthys molitrix (Silver carp) and Barbodes gonionotus (Sar puti) fingerlings were studied separately in aquaria by feeding cultured microalgae (Chlorella ellipsoidea, Scenedesmus obliquus and Spirulina platensis) in different combinations for a period of 21 days. Fertilizer factory effluent was used to grow these microalgae. Significantly (p<0.05) higher weight gain of H. molitrix (81.16%), was in the treatment T2 (Rearing by feeding 100% S. platensis) and the minimum percentage (- 4.29%) was obtained in the control (fed on fishmeal and mustered oil cake). Specific growth rate (SGR, %/day) of H. molitrix were determined - 0.21 to 2.84 for all the treatments which was significantly (p<0.05) higher in T2. Survival rate of H. molitrix was 25.90 to 77.80%. In the case of B. gonionotus, significantly (p<0.05) higher weight gain (149.10%) was observed in t2 (Rearing by feeding 100% S. platensis). The SGR (%/day) of B. gonionotus were ranged from 2.25 to 4.33 which was significantly (p<0.05) higher in t2 than all other treatment except control. Survival rate of B. gonionotus was ranged from 97.78% to 100%. The reared fingerlings were found nutritionally rich. Key words: Fingerlings; Microalgae; Growth performance; Specific growth rate. DOI: 10.3329/bjsir.v45i4.7328 Bangladesh J. Sci. Ind. Res. 45(4), 315-322, 2010

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PEMANFAATAN Spirulina platensis UNTUK MENINGKATKAN KINERJA PERTUMBUHAN DAN KETAHANAN TUBUH IKAN ZEBRA (Danio rerio)

ZIRAA'AH MAJALAH ILMIAH PERTANIAN ◽

10.31602/zmip.v46i2.4187 ◽

2021 ◽

Vol 46 (2) ◽

pp. 211

Author(s):

Lukman Anugrah Agung ◽

Muh. Herjayanto ◽

Eltis Panca Ningsih ◽

Edo Ahmad Solahudin ◽

Esa Rama Widiyawan

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Growth Performance ◽

Experimental Research ◽

Danio Rerio ◽

Spirulina Platensis ◽

Specific Growth ◽

Stress Test ◽

Low Ph

The purpose of this research is to evaluate the effect of different concentration of Spirulina platensis supplementation on artificial feed for zebrafish (Danio rerio) to increase growth performance and immunity from environmental pressure. The experimental research was carried out from October until November 2020. The purpose of this research is to evaluate the effect of S. platensis different concentration supplementation on artificial feed for zebrafish (Danio rerio) to increase growth performance and immunity from environmental pressure. The experimental research was carried out from October 2020 until November 2020 at Aquaculture Laboratory, Universitas Sultan Ageng Tirtayasa, Serang, Banten. This research used a complete randomized design (CRD) consisted of 4 treatments and 3 replications. Control was commercial pellet which no S. platensis enriched (A), pellet enriched with 3% of S.platensis (B), pellet enriched with 6% of S. platensis(C), and last treatment were pellet enriched with 9% of S.platensis (D). The results showed that treatment C was the best dosage of S. platensis for zebrafish rearing for 40 days both growth and immunity performance. Absolute length, Specific growth rate of length, Absolute weight, Specific growth rate of weight were significantly different, but survival rate were not significantly different which were 0.646±0.112g, 0.714±0.10g, 0.102±0.020%, 1.422±0.215%, 100% respectively. Immunity performance was shown by the stress test at low pH. It was also significantly different when exposed to pH at 5 ppt among the treatment. Treatment C was the highest survival rate which was 63.33±5.77% after stress test at low pH.

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Effects of pH on physiological parameters of blood and growth performance of Asian

The Journal of Agriculture and Development ◽

10.52997/jad.11.01.2019 ◽

2019 ◽

Vol 18 (01) ◽

pp. 89-97

Author(s):

Tuan V. Vo

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Growth Performance ◽

Specific Growth ◽

Physiological Parameters ◽

Water Levels ◽

Daily Weight Gain ◽

Effects Of Ph ◽

Set Up

Effects of pH on blood physiological parameters and growth performance of Asian bumblebee catfish (Pseudomystus siamensis) were carried out in laboratory conditions. The experiment was set up in 8 weeks at different pH water levels. The results showed that the cumulative mortality of Asian bumblebee catfish (4 - 6 g/fish) in 24 hpi was 100% at pH = 11, 70.83% at pH = 10 and 62.5% at pH = 3. No mortality of fish was observed at pH = 4, 5, 6, 7, 8, 9 at 24 hpi. The high content of glucose was recorded in fish cultured in water with low (4.02 mmol/L at pH = 3) and high (3.22 mmol/L at pH = 10) pH levels. After 8 weeks of culture, the highest content of glucose was observed at pH = 8 (1.10 mmol/L). The daily weight gain and specific growth rate in weight of fish were 0.02 - 0.08 g/day and 0.35 - 0.99%/day, respectively. The daily length gain specific growth rate in length were 0.02 - 0.04 cm/day and 0.22 - 0.53%/day. The highest survival rate of fish was recorded at pH = 6 (95.96%) and the lowest survival rate of fish was recorded at pH = 8 (60%).

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ANALISIS KARAKTER REPRODUKSI DAN PERFORMA BENIH HIBRID IKAN NILA PANDU F6 DENGAN IKAN NILA NILASA (Oreochromis niloticus) Analysis of the Character Reproduction and Seed Hybrid Performance 0f Tilapia Fish F6 Pandu with Nilasa Tilapia (Oreochromis niloticus)

SAINTEK PERIKANAN Indonesian Journal of Fisheries Science and Technology ◽

10.14710/ijfst.12.1.19-23 ◽

2017 ◽

Vol 12 (1) ◽

pp. 19

Author(s):

Dio Patria Yustysi ◽

Fajar Basuki ◽

Titik Susilowati ◽

Tristiana Yuniarti

Keyword(s):

Water Quality ◽

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Egg Size ◽

Specific Growth ◽

Fish Farming ◽

Hatching Rate ◽

Feed Conversion

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance

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PERTUMBUHAN DAN SINTASAN HIDUP IKAN NILA DENGAN PENAMBAHAN TEPUNG WORI (Ormocarpum cochinchinense) FERMENTASI DALAM PAKAN

Jurnal Ilmiah Tindalung ◽

10.54484/jit.v7i2.370 ◽

2021 ◽

Vol 7 (2) ◽

pp. 1-6

Author(s):

Numisye Iske Mose ◽

Jetti Treslah Saselah

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Specific Growth ◽

Fish Growth ◽

Utilization Efficiency ◽

Fish Feed ◽

Randomized Design ◽

Leaf Powder ◽

Feed Utilization Efficiency

Tanaman wori merupakan salah satu tanaman kelompok fabaceace yang tumbuh melimpah di Sangihe dan sangat potensial digunakan sebagai salah satu sumber pakan ikan. Kadar protein daun wori adalah 25,53% sehingga berpotensi menjadi protein nabati bagi ikan. Kendala utama yang ditemui ketika memanfaatkan daun tanaman wori adalah kandungan karbohidrat yang tinggi yaitu 48,51% sehingga dapat mempengaruhi proses pencernaan ikan, penyerapan nutrisi yang berujung pada rendahnya pertumbuhan ikan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung daun wori femerntasi terhadap laju pertumbuhan spesifik dan sintasan hidup ikan nila. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Perlakuan yang dilakukan terdiri dari tiga perlakuan dan tiga kali ulangan yaitu kadar tepung daun wori tanpa fermentasi A (kontrol) 10%, kemudian B tepung daun wori fermentasi (10%), dan C tepung daun wori fermentasi (20%). Laju pertumbuhan spesifik dan efisiensi pakan diuji menggunakan ANOVA sedangkan sintasan hidup akan dianalisis secara deskriptif. Hasil penelitian ini menunjukan bahwa penambahan tepung wori fermenasi dalam pakan dapat meningkatkan nilai laju pertumbuhan spesfik ikan nila dan efisiensi pemanfaatan pakan dengan nilai tertinggi pada perlakuan C yaitu SGR 1,94%/hari. Sementara itu, untuk semua perlakuan nilai sintasan hidup sebesar 100%. The wori plant is one of the plants of the fabaceace group that grows abundantly in Sangihe and has potential to be used as a source of fish feed. The protein content of wori leaves is 25.53%, so it has the potential to become vegetable protein for fish. The main obstacle encountered when utilizing wori plant leaves is the high carbohydrate content of 48.51% so that it can affect the digestion process of fish, nutrient absorption which lead to low fish growth. The purpose of this study was to determine the effect of wori femerntation leaf meal on the specific growth rate and survival of tilapia. The experimental design used was a completely randomized design (CRD). The treatments consisted of three treatments and three replications, namely the content of fermented wori leaf powder, A (control) 10%, then B fermented wori leaf powder (10%), and C fermented wori leaf powder (20%). Specific growth rate and feed efficiency were tested using ANOVA, while survival rate was analyzed descriptively. The results of this study indicate that the addition of fermented wori powder in the feed can increase the value of the specific growth rate of tilapia and feed utilization efficiency with the highest value in treatment C, namely SGR 1.94% / day. Meanwhile, for all treatments the survival rate was 100%.

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Pengaruh Pemberian Probiotik Berbeda dalam Sistem Akuaponik terhadap Laju Pertumbuhan dan Survival Rate Ikan Lele (Clarias sp.)

Journal of Aquaculture Science ◽

10.31093/joas.v1i1.1 ◽

2017 ◽

Vol 1 (1) ◽

Author(s):

Ayu Herdianti Primashita ◽

Boedi Setya Rahardja ◽

Prayogo .

Keyword(s):

Water Quality ◽

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Water Use ◽

Specific Growth ◽

Final Conclusion ◽

Randomized Design ◽

Business Efficiency ◽

Range Test

Catfish (Clarias sp.) is a freshwater fish that is widely cultivated because it is a leading commodity. Increased production of catfish resulted in extra area of cultivated land and water use. The technology suitable to be applied is aquaponic system. In addition to saving land and water use, Aquaponic is also improving business efficiency through the utilization of nutrients from food remains and fish metabolism. Water quality is an important factor for the success of cultivation. Maintenance catfish with the addition of probiotics in aquaponic system can be a solution for maintaining water quality, because it contains bacteria that can increase nitrite to nitrate changes that can be utilized for growth of plants and do not poison the fish. The purpose of this study was to determine the effect of different probiotics in the aquaponic system towards growth rate and survival rate of catfish. The method that used in this research is experimental with completely randomized design (four treat and five repeated) are P0 (control), P1 (probiotic A), P2 (probiotic B) and P3 (probiotic C). Analysis of data processed using Analysis of Variance. If there are significant differences then continued Duncan's Multiple Range Test. The results showed that the giving of probiotics in the aquaponic system is significantly different (p <0.05). The final conclusion is that the addition of probiotics in aquaponic system affect the growth rate and survival rate of catfish. The highest specific growth rate (0.025%/ day) and the highest survival rate (77.8%) contained in the P2 treatment. The lowest specific growth rate (0.019% /day) and the lowest survival rate (64.4%) contained in P0 treatment (control). Keywords : Aquaponic, Catfish, Probiotic, Growth Rate, Survival Rate, Clarias sp.

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APLIKASI TEPUNG TESTIS SAPI YANG MENGANDUNG rGH DALAM PAKAN BUATAN TERHADAP RASIO JENIS KELAMIN, PERTUMBUHAN DAN KELULUSHIDUPAN IKAN NILA (Oreochromis niloticus)

Pena Akuatika Jurnal Ilmiah Perikanan dan Kelautan ◽

10.31941/penaakuatika.v17i2.656 ◽

2018 ◽

Vol 17 (2) ◽

Author(s):

Rachmat Nurul Huda ◽

Titik Sulistiowati ◽

Tristiana Yuniarti

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Sex Ratio ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Specific Growth ◽

Sex Reversal ◽

Average Weight ◽

Fish Feed ◽

Male Sex

ABSTRAKBudidaya ikan nila (Oreochromis niloticus) terus dikembangkan hingga saat ini. Berbagai teknologi dalam bidang perikanan telah diterapkan, salah satunya yaitu sex reversal dalam hal ini jantanisasi. Ikan nila jantan lebih cepat pertumbuhannya dari pada ikan nila betina sehingga dapat meningkatkan hasil produksi. Tujuan penelitian ini yaitu untuk mengetahui pengaruh pemberian tepung testis sapi dan dosis terbaik tepung testis sapi dalam pakan yang mengandung rGH terhadap rasio jenis kelamin, pertumbuhan dan kelulushidupan ikan nila. Bahan uji yang digunakan adalah larva ikan nila dengan stadia larva yang berumur 7 hari setelah lepas kuning telur dan memiliki bobot rata-rata 0,07 gram/ekor. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini yaitu dengan menambahkan tepung testis sapi dengan dosis pada perlakuan A (0%/kg pakan), perlakuan B (3%/kg pakan), perlakuan C (6%/kg pakan) dan, perlakuan D (9%/kg pakan). Pakan pada setiap perlakuan dalam penelitian ini telah ditambahkan rGH dengan dosis 2 mg/kg pakan. Data yang diamati meliputi rasio jenis kelamin, laju pertumbuhan spesifik (SGR), dan tingkat kelulushidupan (SR) ikan nila. Hasil penelitian menunjukan bahwa perlakuan C (6% tepung testis sapi/kg pakan) memberikan hasil terbaik dimana nilai rasio jenis kelamin jantan sebesar 74,44±5,09% dan laju pertumbuhan spesifik sebesar 9,48±0,10% bobot/hari. Kesimpulan yang diperoleh dalam penelitian ini yaitu adanya pengaruh nyata terhadap rasio jenis kelamin dan tingkat pertumbuhan spesifik, namun tidak berpengaruh nyata terhadap tingkat kelulushidupan ikan nila dengan pemberian dosis tepung testis sapi terbaik adalah 6% tepung testis sapi/kg pakan.Kata kunci: Sex reversal; Tepung testis sapi; Pertumbuhan, rGH; NilaABSTRACTTilapia fish (Oreochromis niloticus)cultivation still continue to be developed until nowdays. Many fisheries technology had been applied, and one of them is sex reversal which is masculinization. Male tilapia fish grows faster than the female one, so this can boost the produce of the fish. This research aims to know the effect of cow testicle flour addition and determine the best dosage on the fish feed which contains rGH towards the male-sex ratio, growth, and survival rate of tilapia fish. The material which used in this research was tilapia fish (O. niloticus) which under the larvae phase 7 days post the separated egg yolk which have average weight of 0,07 grams/each. This research used experimental methods with Completely Randomized Design of 4 treatments and 3 times of repetitions. The treatments in this research was addition of different dosage of bull testicle which treatment A (0%/kg fish feed),treatment B (3%/kg fish feed), treatment C (6%/kg fish feed) and, treatment D (9%/kg fish feed). The fish feed in every treatment had been added with 2mg rGH/kg fish feed. Data observation during this research were male-sex ratio, specific growth rate (SGR), and survival rate (SR) of tilapia fish. Results of the research showed that treatment C (6%/kg fish feed) gave the best result as male-sex ratio by 74,44±5,09% and specific growth rate by 9,48±0,10% weight/day. Conclusion obtained from this research was: there was different result towards the male-sex ratio and specific growth rate, but there was no difference between the treatments towards the survival rate, the best bull testicle flour dosage was 6%/kg fish feed. Keywords: Sex reversal, Bull testicle flour, Growth, rGH, Tilapia

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