Determination of Genetic Diversity inCoccinella septempunctataL. (Coleoptera: Coccinellidae) Associated with Alfalfa,Medicago sativaL., in Two Egyptian Oases, using RAPD-PCR

2014 ◽  
Vol 22 (1) ◽  
pp. 197-203
Author(s):  
G.M. El Bassiony ◽  
U.M.H.M. Abu El-Ghiet
Keyword(s):  
Genetic Diversity ◽  
Rapd Pcr

scholarly journals TANAMAN INDIKATOR DAN TEKNIK RAPD-PCR UNTUK PENENTUAN BIOTIPE BEMISIA TABACI GENNADIUS (HEMIPTERA: ALEYRODIDAE)

2011 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Purnama Hidayat ◽  
Noor Aidawati ◽  
Sri Hendrastuti Hidayat ◽  
Dewi Sartiami
Keyword(s):  
Genetic Diversity ◽  
Bemisia Tabaci ◽  
Virus Vector ◽  
Indicator Plant ◽  
Chilli Pepper ◽  
Genetic Types ◽  
Rapd Pcr ◽  
Major Pest ◽  
Biotype B

Indicator Plant and PCR-RAPD for Biotype Determination of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae).B. tabaci has been known world wide as a major pest and virus vector of horticulture. In Indonesia the presence of B.tabaci was reported since 1980 and its role as virus vector in tomato and chilli pepper has becoming more importantrecently. Genetic diversity of B. tabaci has been well recognized, but very little information available for diversity of B.tabaci in Indonesia. This research was conducted in Bogor, West Java from May 2004 to June 2005. The aim of thisresearch was to initiate basic information regarding genetic diversity of B. tabaci in Indonesia, particularly in Java Island.Whiteflies population collected from different crops, i.e. tomato, broccoli, chill pepper, eggplant, cucumber, soybean, andedamame, was evaluated using silverleaf-induction test, and RAPD-PCR. It was evidenced that only B. tabaci populationfrom broccoli was able to induce silverleaf. Two genetic types of B. tabaci, i.e. biotype B and non B, were identified basedon polymorphism character of DNA. Population from broccoli was belong to biotype B, whereas other populations fromtomato, chill pepper, eggplant, cucumber, soybean, and edamame were belong to biotype non B.

scholarly journals Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR

2021 ◽  
pp. 1-8
Author(s):  
Riva Hafidah ◽  
Ayi Yustiati ◽  
Yuniar Mulyani ◽  
Ibnu Bangkit Bioshina Suryadi
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Descriptive Analysis ◽  
Similarity Index ◽  
Quantitative Descriptive Analysis ◽  
Rapd Pcr ◽  
Result Show ◽  
Pcr Method ◽  
Quantitative Descriptive

This research aims to determine genetic diversity of four strains guppy, respectively are japan blue double sword (JBD), japan blue tiger double sword (JBTD), blue moscow (BM), and panda guppy (PG) with RAPD-PCR method. The obtained genetic diversity data is used as guide reference for hybridization between four strains. The research was conducted in September 2020 to April 2021 with explorative methods and in qualitative and quantitative descriptive analysis. The research was carried out in biotechnology Laboratory, Fishery and Marine Sciences Faculty and Central Laboratory,Padjadjaran University, Indonesia.Strains of JBD, JBTD, BM obtained fromCilengkrangSubdistrict, Bandung and PG strain obtained from Parung market, Bogor. Primary OPA-03 (AGTCAGCCAC) is used for standard parameters to interpret genetic diversity among four strains of guppy. Based on results, amplification with OPA-03 primary visualize 25 bands that include five polymorphic bands and 20 monomorphic bands. The phylogenetic tree result show that there are two relationship groups. The first group are JBD, JBTD, and BM with similarity index in the range of 80-89%The first group consist two sub groups of relationship. The first sub group are JBD and JBTD with similarity index of 89%. The second sub group is BM with similarity index of 80%. The second group isPG with similarity index of 65.5%.

scholarly journals Antibacterial Effects of Octenidine Dihydrochloride, and Benzalkanium Chloride on Acinetobacter Baumannii Strains Isolated from Clinical Samples and Determination of Genetic Diversity of Isolates by RAPD-PCR Method

2021 ◽  
Author(s):  
Azar Dokht Khosravi ◽  
Effat Abasi Montazeri ◽  
Seyedeh Roya Maki
Keyword(s):  
Genetic Diversity ◽  
Acinetobacter Baumannii ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Typing Method ◽  
Conventional Culture ◽  
Rapd Pcr ◽  
Octenidine Dihydrochloride ◽  
Pcr Method

Abstract Due to the emergence of antibiotic resistance in Acinetobacter baumannii which is one of the important causes of nosocomial infections, many problems have been raised in the successful treatment of patients with the subsequent mortality. So, the present study was performed to evaluate the antibacterial effect of Actinidine dehydrochloride, Actinisept, and Benzalkanium chloride against Acinetobacter baumannii strains isolated from clinical samples and to determine the genetic diversity of strains by RAPD-PCR. A total of 119 non-duplicate, suspected Acinetobacter baumannii isolates were collected and confirmed by conventional culture and biochemical tests and PCR technique. Susceptibility of the isolates to antibiotics was evaluated by standard Antibiotic susceptibility testing (AST). For antiseptics Octenidine dihydrochloride (OCT), Actinisept, and Benzalkonium chloride (BZK), Minimal inhibitory concentration (MIC) was assessed. The prevalence of Qac E and Qac delta E genes related to antiseptics was estimated by PCR. Finally, genetic diversity of strains was determined by RAPD-PCR. All 119 suspected isolates were confirmed as Acinetobacter baumannii using conventional tests and PCR. The isolates were mostly originated from blood samples. In AST, the lowest resistance was seen for ciprofloxacin and gentamicin. The MIC values were reported as OCT (15.26 µg) and BZK (640 µg). The antiseptic genes of qacE and qac ΔE1 were found to be present in 56 (47.05%) and 59 (49.57%) of isolates respectively. RAPD typing method revealed great diversity among A. baumannii isolates, with 37 clusters in isolates from ICU, of which 32 isolates were single and 5 were multiple. In conclusion, considering the increase of resistance to antiseptics, it is of importance to monitor the susceptibility of A. baumannii to antiseptics and to promote antiseptic stewardship in hospitals. Furthermore, in this study great diversity among A. baumannii was observed making it difficult to properly carry out infection control policies. analysis of RAPD-PCR typing results, and we found 37 clusters, among them 32 isolates were single and 5 were multiple. So, the method generated 37 RAPD type which shows great diversity among 57 out of 62 A. baumannii isolates at 80% cutoff.

scholarly journals Determination of Genetic Diversity and Paternity in the Gray-Tailed Vole (Microtus canicaudus) by RAPD-PCR

1998 ◽  
Vol 79 (2) ◽  
pp. 604-611 ◽  
Author(s):  
D. A. Gordon ◽  
D. L. Lattier ◽  
R. N. Silbiger ◽  
J. Torsella ◽  
J. O. Wolff ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Pcr

scholarly journals Genetic Similarity of Avena sativa L. Varieties as an Example of a Narrow Genetic Pool of Contemporary Cereal Species

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1424
Author(s):  
Magdalena Cieplak ◽  
Sylwia Okoń ◽  
Krystyna Werwińska
Keyword(s):  
Genetic Diversity ◽  
Avena Sativa ◽  
Genetic Similarity ◽  
Breeding Programs ◽  
Central European ◽  
Cereal Species ◽  
Genetic Pool ◽  
Selection Of ◽  
High Genetic Similarity

The assessment of the genetic diversity of cultivated varieties is a very important element of breeding programs. This allows the determination of the level of genetic differentiation of cultivated varieties, their genetic distinctiveness, and is also of great importance in the selection of parental components for crossbreeding. The aim of the present study was to determine the level of genetic diversity of oat varieties currently grown in Central Europe based on two marker systems: ISSR and SCoT. The research conducted showed that both these types of markers were suitable for conducting analyses relating to the assessment of genetic diversity. The calculated coefficients showed that the analyzed cultivars were characterized by a high genetic similarity. However, the UPGMA and PCoA analyses clearly indicated the distinctiveness of the breeding programs conducted in Central European countries. The high genetic similarity of the analyzed forms allow us to conclude that it is necessary to expand the genetic pool of oat varieties. Numerous studies show that landraces may be the donor of genetic variation.

ANALYSIS OF GENETIC DIVERSITY OF 26 CASSAVA VARIETIES (Manihot esculenta CRANTZ) WITH DIFFERENT RESPONSES TO WATERLOGGING CONDITIONS BY USING RAPD MARKERS

2021 ◽  
Vol 66 (3) ◽  
pp. 170-179
Author(s):  
Sengsoulichan Dethvongsa ◽  
Vu Nguyen Anh ◽  
Van Tran Khanh
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Flood Tolerance ◽  
Randomly Amplified Polymorphic Dna ◽  
Manihot Esculenta Crantz ◽  
Rapd Pcr ◽  
Cassava Production ◽  
Cassava Varieties

RAPD (Randomly Amplified Polymorphic DNA) is an indicator for high and stable polymorphism, widely used in the study of the diversity of cassava. In this paper, the results of using 20 polymorphic primers OPK combined with the establishment of the phylogenetic tree to analyze the genetic diversity of 26 cassava varieties with different responses to waterlogging conditions by using the RAPD-PCR technique were presented. The purpose of this experiment was to show the genetic relevance of the studied cassava varieties. The results showed that the flood tolerance of cassava was not related to the polymorphism and branching characteristics of the stem. This information may be use as a basis for selecting flood-tolerant cassava varieties for cassava production, as well as the basis for selecting genetically different parents for breeding.

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