scholarly journals Influence of Lactose Concentration in Bioethanol Production from Cheese Whey

2017 ◽  
Vol 39 (5) ◽  
pp. 533
Author(s):  
Cleidiane Samara Murari ◽  
Débora Cristina Moraes Niz da Silva ◽  
Bruna Lima da Silva ◽  
Vanildo Luiz Del Bianchi
Keyword(s):  
Alcoholic Fermentation ◽  
Cheese Whey ◽  
Ethanol Yield ◽  
Oxygen Demand ◽  
Bioethanol Production ◽  
Organic Substances ◽  
Promising Alternative ◽  
Renewable Source ◽  
Initial Concentration ◽  
Lactose Concentration

The present study aimed the utilization of the cheese whey as substrate for ethanol production by Kluyveromyces marxianus.  Was studied the effect of the initial concentration of cheese whey (M1 57,6 g L-1; M2 45,6 g L-1; M3 32,5 g L-1 e M4 18,8 g L-1) on the alcoholic fermentation. After sterilization, the medium were incubated at 30ºC for 48 hours, performing analysis of lactose, proteins, ethanol, cell growth and chemical oxygen demand. According to the results, the medium M1 (the highest concentration) showed a higher production and productivity of ethanol 16.9 g L-1 and 1.26 g L.h-1, respectively, and also obtained the highest production and productivity of cell of 5.8 g L-1 and 0.40 g L.h-1, respectively. However, in terms of ethanol yield, the most satisfactory result was obtained with the M3 medium with 82.30% in 12 hours of fermentation. The organic substances content has been reduced in relation to COD in the medium M4 in 82.28%, representing a promising alternative for valorization of cheese whey as an effective alternative to obtain a renewable source of biofuel.

scholarly journals Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis

2016 ◽  
Vol 2 (1) ◽  
pp. 1-12
Author(s):  
Inda Setyawati ◽  
Laksmi Ambarsari ◽  
Siti Nur'aeni ◽  
Suryani Suryani ◽  
Puspa Julistia Puspita ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Ethanol Yield ◽  
Renewable Resource ◽  
Bioethanol Production ◽  
Fermentation Inhibitors ◽  
Renewable Materials ◽  
Promising Alternative ◽  
Fermentation Time ◽  
Sugarcane Bagasse Hydrolysate

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.

scholarly journals The potentiality of bioethanol production from corn (Zea mays L.) as a renewable source

2020 ◽  
pp. 1-4
Author(s):  
M. N. Yesmin ◽  
M. A. K. Azad ◽  
M. Kamuruzzaman ◽  
S. Ali
Keyword(s):  
Zea Mays ◽  
Alcoholic Fermentation ◽  
Zea Mays L ◽  
Bioethanol Production ◽  
Distillation Process ◽  
Recent Past ◽  
Corn Flour ◽  
Yeast Saccharomyces Cerevisiae ◽  
Corn Production ◽  
Renewable Source

Corn (Zea mays L.)  is one of the versatile crop which is used as food, feed, fodder and in recent past as a source of bio-fuel. The sub-tropical climate is very favorable for corn cultivation. Traditionally, corn was grown in South and Southeast Asia primarily as a subsistence food crop. Worldwide it is being cultivated in over 170 countries representing an area of 185 million ha with a productivity of 5.62 t ha-1 (FAO, 2017). Out of world corn production of 1037 million MT, SAARC countries comprising of Afghanistan, Bangladesh, Bhutan, India, Maldives, Nepal, Pakistan and Sri Lanka represent 3.2 % with a productivity of 3.8 t ha-1. Among SAARC countries, the highest productivity of 6.9 t ha-1 is reported in Bangladesh. Corn can be an important renewable source for bioethanol production. This research was carried out to evaluate Bangladeshi Corn for optimum bioethanol production. A 100 g of corn flour was mixed with 300 ml distilled water was blended and sterilized. The experiment was conducted with a temperature of 30 oC, pH 6.0 and 20 % sugar concentration. For alcoholic fermentation, 200 ml yeast (Saccharomyces cerevisiae CCD) was added to make the total volume 500 ml. Addition of small amount of 1750 unit α-amylase enzyme to the substrate solution was found to enhance the fermentation process quicker. After 6-days of incubation time corn produces 63.57 ml of ethanol with 13.33 % (v/v) purity. The non-filtered solution produces comparatively more ethanol (63.57 ml with 13.33 % purity) than the filtered solution (53.66 ml with 10 % purity). The purity can be increased by re-distillation process.   

A simple test for monitoring biodegradable industrial pollution in a receiving stream

1999 ◽  
Vol 39 (10-11) ◽  
pp. 221-224 ◽  
Author(s):  
Jana Zagorc-Končan ◽  
J. Šömen
Keyword(s):  
Hazard Assessment ◽  
Industrial Pollution ◽  
Self Regulation ◽  
Oxygen Demand ◽  
Organic Pollution ◽  
Organic Substances ◽  
Simple Test ◽  
Industrial Pollutants ◽  
Model Substances ◽  
Receiving Stream

Microbial purification capacity is an important factor in natural self-regulation in water. Evaluating the fate of biodegradable organic pollution downstream from the discharge seems an appropriate way to follow the effect of pollution and its hazard assessment, which dictates the needed sanitation measures. We suggest a simple test for such monitoring. A modification of the additional oxygen demand test, standardised in Ausgewählte Methoden der Wasseruntersuchung, was applied in two river case studies. The additional oxygen demand is a measure of the capability and rate of biodegradation of known organic substance as well as of the amount and activity of heterotrophic organisms in the river. The original test using peptone and glucose as additional feedings of BOD samples was modified by the use of other organic biodegradable model substances characteristic for individual industrial pollutants. The test was found to be an excellent indicator of adapted microorganisms, which are essential for the biodegradation of the appointed organic substances downstream of their discharge into the receiving stream.

scholarly journals Saccharomyces cerevisiae Fermentation of 28 Barley and 12 Oat Cultivars

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 59
Author(s):  
Timothy J. Tse ◽  
Daniel J. Wiens ◽  
Jianheng Shen ◽  
Aaron D. Beattie ◽  
Martin J. T. Reaney
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Succinic Acid ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Cereal Crops ◽  
Fermentation Products ◽  
Barley Cultivars ◽  
Oat Cultivars

As barley and oat production have recently increased in Canada, it has become prudent to investigate these cereal crops as potential feedstocks for alcoholic fermentation. Ethanol and other coproduct yields can vary substantially among fermented feedstocks, which currently consist primarily of wheat and corn. In this study, the liquified mash of milled grains from 28 barley (hulled and hull-less) and 12 oat cultivars were fermented with Saccharomyces cerevisiae to determine concentrations of fermentation products (ethanol, isopropanol, acetic acid, lactic acid, succinic acid, α-glycerylphosphorylcholine (α-GPC), and glycerol). On average, the fermentation of barley produced significantly higher amounts of ethanol, isopropanol, acetic acid, succinic acid, α-GPC, and glycerol than that of oats. The best performing barley cultivars were able to produce up to 78.48 g/L (CDC Clear) ethanol and 1.81 g/L α-GPC (CDC Cowboy). Furthermore, the presence of milled hulls did not impact ethanol yield amongst barley cultivars. Due to its superior ethanol yield compared to oats, barley is a suitable feedstock for ethanol production. In addition, the accumulation of α-GPC could add considerable value to the fermentation of these cereal crops.

scholarly journals Enzymatic Process for Cystoseira barbata Valorization: Ethanol Production and Additional By-Products

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 741
Author(s):  
Doinita-Roxana Cioroiu Tirpan ◽  
Ancaelena Eliza Sterpu ◽  
Claudia Irina Koncsag ◽  
Alina Georgiana Ciufu ◽  
Tănase Dobre
Keyword(s):  
Ethanol Production ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Product Yield ◽  
Liquid Ratio ◽  
Experimental Conditions ◽  
Factors Affecting ◽  
Cystoseira Barbata ◽  
Main Factors ◽  
Process Factors

The aim of this study is to evaluate the potential of dried Cystoseira barbata alga for ethanol production through alcoholic fermentation. The influence of the main factors affecting the fermentation are studied in the frame of a 23 factorial experimental plan. The main factors influencing the process are the fermentation temperature (t from 25 °C to 35 °C), the solid to liquid ratio (S/L from 0.040 g/g to 0.080 g/g), and the cellulase ratio (R from 8 U/g d.m to 16 U/g d.m.). The maximum volatile compounds yield of 0.2808 g/g d.m and ethanol yield of 0.0158 g/g d.m were favored by the following experimental conditions: process temperature of 35 °C, solid to liquid ratio of 0.0415, and enzyme ratio of 16 U/g d.m. A statistical model was used to correlate the product yield with the process factors. Additionally, 19 interesting bioactive compounds were found in the enzymatic hydrolysis and alcoholic fermentation broths which seem likely to maintain natural defence mechanisms against diseases and physical disorders.

scholarly journals Hemicellulosic Bioethanol Production from Fast-Growing Paulownia Biomass

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 173
Author(s):  
Elena Domínguez ◽  
Pablo G. del Río ◽  
Aloia Romaní ◽  
Gil Garrote ◽  
Lucília Domingues
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Xylose Reductase ◽  
Energy Crop ◽  
Scheffersomyces Stipitis ◽  
Fractionation Process ◽  
Renewable Source ◽  
Fast Growing ◽  
Engineered Strain

In order to exploit a fast-growing Paulownia hardwood as an energy crop, a xylose-enriched hydrolysate was obtained in this work to increase the ethanol concentration using the hemicellulosic fraction, besides the already widely studied cellulosic fraction. For that, Paulownia elongata x fortunei was submitted to autohydrolysis treatment (210 °C or S0 of 4.08) for the xylan solubilization, mainly as xylooligosaccharides. Afterwards, sequential stages of acid hydrolysis, concentration, and detoxification were evaluated to obtain fermentable sugars. Thus, detoxified and non-detoxified hydrolysates (diluted or not) were fermented for ethanol production using a natural xylose-consuming yeast, Scheffersomyces stipitis CECT 1922, and an industrial Saccharomyces cerevisiae MEC1133 strain, metabolic engineered strain with the xylose reductase/xylitol dehydrogenase pathway. Results from fermentation assays showed that the engineered S. cerevisiae strain produced up to 14.2 g/L of ethanol (corresponding to 0.33 g/g of ethanol yield) using the non-detoxified hydrolysate. Nevertheless, the yeast S. stipitis reached similar values of ethanol, but only in the detoxified hydrolysate. Hence, the fermentation data prove the suitability and robustness of the engineered strain to ferment non-detoxified liquor, and the appropriateness of detoxification of liquor for the use of less robust yeast. In addition, the success of hemicellulose-to-ethanol production obtained in this work shows the Paulownia biomass as a suitable renewable source for ethanol production following a suitable fractionation process within a biorefinery approach.

scholarly journals The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers

2021 ◽  
Vol 11 (2) ◽  
pp. 510
Author(s):  
Elza R. Mikheeva ◽  
Inna V. Katraeva ◽  
Andrey A. Kovalev ◽  
Dmitriy A. Kovalev ◽  
Alla N. Nozhevnikova ◽  
...  
Keyword(s):  
Cheese Whey ◽  
Hydraulic Retention Time ◽  
Continuous Production ◽  
Oxygen Demand ◽  
Organic Load ◽  
Anaerobic Sludge ◽  
Acid Pretreatment ◽  
Methanogenic Activity ◽  
Start Up ◽  
Load Rate

This article presents the results of the start-up of continuous production of biohydrogen from cheese whey (CW) in an anaerobic filter (AF) and anaerobic fluidized bed (AFB) with a polyurethane carrier. Heat and acid pretreatments were used for the inactivation of hydrogen-scavengers in the inoculum (mesophilic and thermophilic anaerobic sludge). Acid pretreatment was effective for thermophilic anaerobic sludge to suppress methanogenic activity, and heat treatment was effective for mesophilic anaerobic sludge. Maximum specific yields of hydrogen, namely 178 mL/g chemical oxygen demand (COD) and 149 mL/g COD for AFB and AF, respectively, were obtained at the hydraulic retention time (HRT) of 4.5 days and organic load rate (OLR) of 6.61 kg COD/(m3 day). At the same time, the maximum hydrogen production rates of 1.28 and 1.9 NL/(L day) for AF and AFB, respectively, were obtained at the HRT of 2.02 days and OLR of 14.88 kg COD/(m3 day). At the phylum level, the dominant taxa were Firmicutes (65% in AF and 60% in AFB), and at the genus level, Lactobacillus (40% in AF and 43% in AFB) and Bifidobacterium (24% in AF and 30% in AFB).

scholarly journals Recombinant Diploid Saccharomyces cerevisiae Strain Development for Rapid Glucose and Xylose Co-Fermentation

Fermentation ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 59 ◽  
Author(s):  
Tingting Liu ◽  
Shuangcheng Huang ◽  
Anli Geng
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Xylose Isomerase ◽  
Cost Effective ◽  
Xylose Utilization ◽  
Yeast Strains ◽  
Multiple Copies ◽  
Biomass Sugars ◽  
Sugar Conversion

Cost-effective production of cellulosic ethanol requires robust microorganisms for rapid co-fermentation of glucose and xylose. This study aims to develop a recombinant diploid xylose-fermenting Saccharomyces cerevisiae strain for efficient conversion of lignocellulosic biomass sugars to ethanol. Episomal plasmids harboring codon-optimized Piromyces sp. E2 xylose isomerase (PirXylA) and Orpinomyces sp. ukk1 xylose (OrpXylA) genes were constructed and transformed into S. cerevisiae. The strain harboring plasmids with tandem PirXylA was favorable for xylose utilization when xylose was used as the sole carbon source, while the strain harboring plasmids with tandem OrpXylA was beneficial for glucose and xylose cofermentation. PirXylA and OrpXylA genes were also individually integrated into the genome of yeast strains in multiple copies. Such integration was beneficial for xylose alcoholic fermentation. The respiration-deficient strain carrying episomal or integrated OrpXylA genes exhibited the best performance for glucose and xylose co-fermentation. This was partly attributed to the high expression levels and activities of xylose isomerase. Mating a respiration-efficient strain carrying the integrated PirXylA gene with a respiration-deficient strain harboring integrated OrpXylA generated a diploid recombinant xylose-fermenting yeast strain STXQ with enhanced cell growth and xylose fermentation. Co-fermentation of 162 g L−1 glucose and 95 g L−1 xylose generated 120.6 g L−1 ethanol in 23 h, with sugar conversion higher than 99%, ethanol yield of 0.47 g g−1, and ethanol productivity of 5.26 g L−1·h−1.

Treatment of Alkaline Waste Water Generated by the Hydrolysis of Rice Husk

2013 ◽  
Vol 781-784 ◽  
pp. 2087-2090 ◽  
Author(s):  
Ludmila A. Zemnukhova ◽  
O.D. Arefieva ◽  
Anastasia A. Kovshun
Keyword(s):  
Waste Water ◽  
Electrochemical Oxidation ◽  
Rice Husk ◽  
Alkaline Hydrolysis ◽  
Waste Water Treatment ◽  
Oxygen Demand ◽  
Organic Substances ◽  
Silicon Compounds ◽  
Treatment Stage ◽  
Hydrolysis Of

This article shows the results of research on finding ways to purify waste water produced by alkaline hydrolysis of rice waste - rice husk. Two methods were used: baromembrane filtration and electrochemical oxidation of organic substances. It was found that in the results of baromembrane processing of hydrolyzate on the membranes mainly stay silicon compounds partially sorbing organic substances contained in the feedstock. Through the membranes there pass the major part of microbiologically oxidated organic matter, so its difficult to reach the required waste water treatment stage. The use of electrochemical oxidation of organic substances extracted by alkaline hydrolysis of rice husks into a solution allows more qualitatively treat the solution. A range of indicators (chemical oxygen demand, permanganate demand, polyphenols) evaluating the content of organic compounds in the desilicated solution showed that in order to remove them effectively enough electrolyte concentration of sodium chloride is 4 g L-1, formed by the desilication and dilution of the solution with the distilled water 1:10.

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