scholarly journals Enzymatic Process for Cystoseira barbata Valorization: Ethanol Production and Additional By-Products

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 741
Author(s):  
Doinita-Roxana Cioroiu Tirpan ◽  
Ancaelena Eliza Sterpu ◽  
Claudia Irina Koncsag ◽  
Alina Georgiana Ciufu ◽  
Tănase Dobre
Keyword(s):  
Ethanol Production ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Product Yield ◽  
Liquid Ratio ◽  
Experimental Conditions ◽  
Factors Affecting ◽  
Cystoseira Barbata ◽  
Main Factors ◽  
Process Factors

The aim of this study is to evaluate the potential of dried Cystoseira barbata alga for ethanol production through alcoholic fermentation. The influence of the main factors affecting the fermentation are studied in the frame of a 23 factorial experimental plan. The main factors influencing the process are the fermentation temperature (t from 25 °C to 35 °C), the solid to liquid ratio (S/L from 0.040 g/g to 0.080 g/g), and the cellulase ratio (R from 8 U/g d.m to 16 U/g d.m.). The maximum volatile compounds yield of 0.2808 g/g d.m and ethanol yield of 0.0158 g/g d.m were favored by the following experimental conditions: process temperature of 35 °C, solid to liquid ratio of 0.0415, and enzyme ratio of 16 U/g d.m. A statistical model was used to correlate the product yield with the process factors. Additionally, 19 interesting bioactive compounds were found in the enzymatic hydrolysis and alcoholic fermentation broths which seem likely to maintain natural defence mechanisms against diseases and physical disorders.

Fresh Ulva Lactuca Alcoholic Fermentation Products and yields

2018 ◽  
Vol 69 (5) ◽  
pp. 1226-1228
Author(s):  
Doinita Roxana Cioroiu ◽  
Claudia Irina Koncsag ◽  
Dragos Gudovan ◽  
Tanase Dobre
Keyword(s):  
Experimental Data ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Fermentation Broth ◽  
Ulva Lactuca ◽  
Regression Equations ◽  
Optimal Parameters ◽  
Fermentation Products ◽  
Factors Affecting ◽  
Process Factors

The optimization of the ethanol yield obtained by alcoholic fermentation of fresh Ulva lactuca in the presence of cellulase and Saccharomyces cerevisiae yeast was performed. Three main factors affecting the process were considered: the solid-to-liquid ratio (S), cellulase-to-algal biomass ratio (R), expressed as cellulase units (U) per gram of dry matter (DM), and the alcoholic fermentation temperature (t). The optimal parameters were: S=1/24 g/g, R=16 U/ g DM and t=35�C, when alcohol yield was 0.34 g/100 g fresh alga, within the range of other authors� experimental data. Over 200 compounds were identified in the distillate from the fermentation broth, recommending Ulva lactuca as a sustainable source of drugs, intermediates for biosyntheses and biomaterials. The yield of these compounds was 5.92 g/100 g fresh alga, in the same conditions. The yields of ethanol (0.013-0.023 g/g DM) and volatile compounds (0.199-0.398 g/g DM) were predicted depending on the process factors using regression equations obtained by ANOVA analysis of experimental data.

scholarly journals Fermentation of sucrose supplemented with monomeric sugars

1969 ◽  
Vol 74 (1) ◽  
pp. 45-50
Author(s):  
Angel Moret Figueroa ◽  
Carlos Basilio Reyes
Keyword(s):  
Ethanol Production ◽  
Zymomonas Mobilis ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Basic Medium ◽  
Alcohol Production ◽  
Caribbean Area ◽  
Final Yield ◽  
The Caribbean ◽  
Fermentation Period

Zymomonas mobilis is a bacterium which presents several advantages over yeast with respect to ethanol production. It ferments monosaccarides with higher yields and tolerates higher concentrations of ethanol and sugars. However, the alcoholic fermentation of sucrose, which is the most widely used sugar for alcohol production in the Caribbean area, is poor. Therefore, it is important to find how to increase ethanol yield by using Zymomonas mobilis with sucrose as starting material. Our experimental results demonstrated that the addition of monosaccharides to a basic medium containing 6% sucrose increased ethanol yield by at least 10%. The addition of 2% fructose at the beginning of the fermentation period increased ethanol yield from 87.4% to 100%, whereas the addition of 2% glucose produced a yield of 97.5%. When 1% of both monosaccharides was added the final yield was 99.8%.

scholarly journals Engineered Polyploid Yeast Strains Enable Efficient Xylose Utilization and Ethanol Production in Corn Hydrolysates

2021 ◽  
Vol 9 ◽  
Author(s):  
Lulu Liu ◽  
Mingjie Jin ◽  
Mingtao Huang ◽  
Yixuan Zhu ◽  
Wenjie Yuan ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Comprehensive Evaluation ◽  
Ethanol Yield ◽  
Xylose Utilization ◽  
Acid Pretreatment ◽  
Experimental Conditions ◽  
Total Sugars ◽  
Yeast Strains ◽  
Ethanol Yields ◽  
Substrate Concentrations

The reported haploid Saccharomyces cerevisiae strain F106 can utilize xylose for ethanol production. After a series of XR and/or XDH mutations were introduced into F106, the XR-K270R mutant was found to outperform others. The corresponding haploid, diploid, and triploid strains were then constructed and their fermentation performance was compared. Strains F106-KR and the diploid produced an ethanol yield of 0.45 and 0.48 g/g total sugars, respectively, in simulated corn hydrolysates within 36 h. Using non-detoxicated corncob hydrolysate as the substrate, the ethanol yield with the triploid was approximately sevenfold than that of the diploid at 40°C. After a comprehensive evaluation of growth on corn stover hydrolysates pretreated with diluted acid or alkali and different substrate concentrations, ethanol yields of the triploid strain were consistently higher than those of the diploid using acid-pretreatment. These results demonstrate that the yeast chromosomal copy number is positively correlated with increased ethanol production under our experimental conditions.

scholarly journals Transcriptional factor Cat8 is involved in regulation of xylose fermentation in engineered Saccharomyces cerevisiae

2018 ◽  
Vol 22 ◽  
pp. 329-334
Author(s):  
L. S. Dzanaieva ◽  
K. V. Dmytruk ◽  
A. A. Sybirny
Keyword(s):  
Ethanol Production ◽  
Alcoholic Fermentation ◽  
Xylose Fermentation ◽  
Ethanol Yield ◽  
Knock Out ◽  
Coding Regions ◽  
Gene Coding ◽  
Engineered Saccharomyces Cerevisiae ◽  
Deletion Cassette ◽  
Ethanol Synthesis

Aim. The aim of this work is the construction of cat8Δ strain on the base of xylose-fermenting S. cerevisiae strain and evaluation of the xylose fermentation rate. Methods. The CAT8 deletion cassette harboring natNT2 gene flanking with 5’ and 3’ non-coding regions of CAT8 gene has been constructed. After transformation by the cassette the cat8Δ strain was selected on the nourseothricin containing medium. Xylose fermentation experiments of constructed strain was performed in mineral medium supplemented with xylose under oxygen-limited conditions. Results. Xylose-fermenting cat8Δ S. cerevisiae strain has been constructed by homologous recombination of the CAT8 deletion cassette with target sequences in the genome of GS010 strain. The cat8Δ strain possessed increase in ethanol accumulation, ethanol yield, rate of ethanol production and productivity of ethanol synthesis relative to the parental GS010 strain for 9.5, 6, 20 and 12 %, respectively. Conclusions. The mutant of the xylose-fermenting S. cerevisiae strain with knock out of the CAT8 gene coding for transcriptional activator, has been constructed. The cat8Δ mutant showed 9.5 % increase in ethanol production from xylose relative to parental strain. Keywords: alcoholic fermentation, xylose, S. cerevisiae, Cat8.

Mollusk fauna associated with Cystoseira barbata (Stockhouse) C. Agardh, 1820 in the Sea of Marmara (Turkey)

2019 ◽  
Vol 48 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Banu Bitlis
Keyword(s):  
New Record ◽  
Sea Of Marmara ◽  
Factors Affecting ◽  
Number Of Species ◽  
Mollusk Fauna ◽  
Cystoseira Barbata ◽  
Wet Weight ◽  
Main Factors ◽  
Mytilaster Lineatus ◽  
Mollusk Species

Abstract Mollusk species associated with Cystoseira barbata were examined in the Sea of Marmara in 2012 at 14 sampling sites with a depth ranging from 0.2 to 0.5 m. A total of 18 468 specimens belonging to three classes (Polyplacophora, Gastropoda and Bivalvia) and 30 species were identified. Alvania mamillata Risso, 1826 is a new record for the marine mollusk fauna of the Sea of Marmara. The number of species at the sampling sites varied from 2 to 11 (400 cm–2), the density ranged between 758 and 63 083 ind. m–2, and wet weight between 0.4 and 34.3 g m–2. Gastropoda were the richest class, represented by 22 species and 7611 individuals. The families Rissoidae, Mytilidae and Pyramidellidae were represented by the largest number of species. The most dominant mollusk species in C. barbata facies were Mytilaster minimus (Poli, 1795) (37.77%), Rissoa splendida Eichwald, 1830 (27.20%), Mytilaster lineatus (Gmelin, 1791) (18.69%) and Bittium reticulatum (da Costa, 1778) (6.89%). However, the mollusks R. splendida (86%), M. lineatus (71%), M. minimus (69%) and Steromphala adansonii (Payraudeau, 1826) (67%) were characterized by the highest values of the frequency index. Canonical correspondence analysis showed that the main factors affecting the assemblages of mollusks were the content of total nitrogen, the maximum thallus height, algal wet weight and temperature

scholarly journals Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of l-Arabinose

2007 ◽  
Vol 73 (15) ◽  
pp. 4881-4891 ◽  
Author(s):  
H. Wouter Wisselink ◽  
Maurice J. Toirkens ◽  
M. del Rosario Franco Berriel ◽  
Aaron A. Winkler ◽  
Johannes P. van Dijken ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Alcoholic Fermentation ◽  
Plant Biomass ◽  
Ethanol Yield ◽  
Dry Weight ◽  
Pentose Phosphate ◽  
Anaerobic Growth ◽  
Evolutionary Engineering ◽  
First Case

ABSTRACT For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as l-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production, cannot ferment xylose and arabinose. Although metabolic and evolutionary engineering has enabled the efficient alcoholic fermentation of xylose under anaerobic conditions, the conversion of l-arabinose into ethanol by engineered S. cerevisiae strains has previously been demonstrated only under oxygen-limited conditions. This study reports the first case of fast and efficient anaerobic alcoholic fermentation of l-arabinose by an engineered S. cerevisiae strain. This fermentation was achieved by combining the expression of the structural genes for the l-arabinose utilization pathway of Lactobacillus plantarum, the overexpression of the S. cerevisiae genes encoding the enzymes of the nonoxidative pentose phosphate pathway, and extensive evolutionary engineering. The resulting S. cerevisiae strain exhibited high rates of arabinose consumption (0.70 g h−1 g [dry weight]−1) and ethanol production (0.29 g h−1 g [dry weight]−1) and a high ethanol yield (0.43 g g−1) during anaerobic growth on l-arabinose as the sole carbon source. In addition, efficient ethanol production from sugar mixtures containing glucose and arabinose, which is crucial for application in industrial ethanol production, was achieved.

Recent Rapid Climate Changes in Antarctic and their Influence on Low Diversity Ecosystems

2010 ◽  
Vol 17 (1) ◽  
pp. 17-30 ◽  
Author(s):  
Katarzyna J. Chwedorzewska
Keyword(s):  
Environmental Variables ◽  
Climate Changes ◽  
Biological Significance ◽  
Global Changes ◽  
Factors Affecting ◽  
Low Diversity ◽  
Main Factors ◽  
The Antarctic ◽  
High Diversity ◽  
Temperature Water

ABSTRACTThe geographic position, astronomic factors (e.g. the Earth’s maximum distance from the Sun during winter), ice cover and altitude are the main factors affecting the climate of the Antarctic, which is the coldest place on Earth. Parts of Antarctica are facing the most rapid rates of anthropogenic climate change currently seen on the planet. Climate changes are occurring throughout Antarctica, affecting three major groups of environmental variables of considerable biological significance: temperature, water, UV-B radiation.Low diversity ecosystems are expected to be more vulnerable to global changes than high diversity ecosystems

The Chemical Decontamination Process Intensification, using Ultrasounds

2008 ◽  
Vol 59 (9) ◽  
Author(s):  
Eugenia Pantru ◽  
Gheorghit Jinescu ◽  
Rozalia R�dulescu ◽  
Antoneta Filcenco Olteanu ◽  
Cosmin Jinescu
Keyword(s):  
Chemical Composition ◽  
Process Intensification ◽  
Sulphuric Acid Solution ◽  
Liquid Ratio ◽  
Polluted Soils ◽  
Chemical Decontamination ◽  
First Case ◽  
Ultrasound Field ◽  
Different Types ◽  
Main Factors

This paper presents an intensive procedure used for the decontamination of the soils, which were radioactively contaminated by uranium, due to the occurrence of some antropic accidents, in order to limit the area�s pollution. The procedure used for the chemical decontamination of the polluted soils was the washing one and the decontamination degree is comparatively presented depending on the ultrasounds� presence and absence. The lab testes were performed on five types of soils , which were characterized from the granulometric, structural and chemical composition viewpoint, all these aspects represent the main factors, which determine the applied decontamination procedure�s limits and performances correlated with its utilization costs. The decontamination procedure�s kinetics for each type of soils was analyzed, using successively three different types of reagents (water, 0.1 M sulphuric acid solution and chloro-sodic solution � 100 g/L sodium chloride + 10 g/L sodium carbonate in water) for a solid to liquid ratio of 1:2, during 2 h, at a temperature of 20oC in a mechanic stirring system respectively in ultrasounds field. It was observed that the decontamination degree increases with up to 15-20% in case of the ultrasound field utilization comparing to the first case.

Pitting Corrosion of Biomedical Titanium and Titanium Alloys: A Brief Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Yu-Wei Cui ◽  
Liang-Yu Chen ◽  
Xin-Xin Liu
Keyword(s):  
Corrosion Resistance ◽  
Pitting Corrosion ◽  
Potential Temperature ◽  
Growth Stages ◽  
Factors Affecting ◽  
Ti Alloys ◽  
Pitting Corrosion Resistance ◽  
Superior Mechanical Properties ◽  
Good Biocompatibility ◽  
Main Factors

Abstract:: Thanks to their excellent corrosion resistance, superior mechanical properties and good biocompatibility, titanium (Ti) and Ti alloys are extensively applied in biomedical fields. Pitting corrosion is a critical consideration for the reliability of Ti and Ti alloys used in the human body. Therefore, this article focuses on the pitting corrosion of Ti and Ti alloys, which introduces the growth stages of pitting corrosion and its main influencing factors. Three stages, i.e. (1) breakdown of passive film, (1) metastable pitting, and (3) propagation of pitting, are roughly divided to introduce the pitting corrosion. As reviewed, corrosive environment, applied potential, temperature and alloy compositions are the main factors affecting the pitting corrosion of Ti and Ti alloys. Moreover, the pitting corrosion of different types Ti alloys are also reviewed to correlate the types of Ti alloys and the main factors of pitting corrosion. Roughly speaking, β-type Ti alloys have the best pitting corrosion resistance among the three types of Ti alloys.

scholarly journals Measuring the Efficiency of Football Clubs Using Data Envelopment Analysis: Empirical Evidence From Spanish Professional Football

SAGE Open ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 215824402198925
Author(s):  
Isidoro Guzmán-Raja ◽  
Manuela Guzmán-Raja
Keyword(s):  
Data Envelopment Analysis ◽  
High Efficiency ◽  
Sport Performance ◽  
Data Envelopment ◽  
Professional Football ◽  
Efficiency Measure ◽  
Factors Affecting ◽  
Football Clubs ◽  
Main Factors ◽  
Using Data

Professional football clubs have a special characteristic not shared by other types of companies: their sport performance (on the field) is important, in addition to their financial performance (off the field). The aim of this paper is to calculate an efficiency measure using a model that combines performance (sport and economic) based on data envelopment analysis (DEA). The main factors affecting teams’ efficiency levels are investigated using cluster analysis. For a sample of Spanish football clubs, the findings indicate that clubs achieved a relatively high efficiency level for the period studied, and that the oldest teams with the most assets had the highest efficiency scores. These results could help club managers to improve the performance of their teams.

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