Optimization of Culture Conditions for Biosynthesis of Lipopeptide by Bacillus Subtilis with Starch

2013 ◽  
Vol 291-294 ◽  
pp. 225-229 ◽  
Author(s):  
Zong Ming Zheng ◽  
Ti Peng Wang ◽  
Qin Wu
Keyword(s):  
Bacillus Subtilis ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Ph Optimum ◽  
Linear Effect ◽  
Initial Ph ◽  
Culture Cycle ◽  
Optimization Of Culture Conditions ◽  
Fermentation Parameters ◽  
Main Effects

Optimization of culture conditions for lipopeptide production of lipopeptide by Bacillus subtilis NEL-01 was carried out in shaker flask batch fermentations using composite central design of response surface methodology (RSM). A five-level three-factor central composite design was employed to determine the maximum lipopeptide production at optimum levels for culture temperature, initial pH and culture cycle. Culture temperature and culture cycle showed the significant linear main effects, while pH had no significant linear effect. The production was also significantly affected by quadratic effect of culture temperature and initial pH. Optimum fermentation parameters were predicted at temperature, initial pH, and culture cycle of 34.81 °C, 7.33g/l, 49.26 h, respectively. The prediction lipopeptide yield was 1879.56 mg/l. The subsequent experiments confirmed the prediction.

scholarly journals Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient StrainBacillus subtilisUJS0717

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Zhuan Wei ◽  
Jue Zhou ◽  
WenJing Sun ◽  
FengJie Cui ◽  
QinHua Xu ◽  
...  
Keyword(s):  
Corn Starch ◽  
Corn Steep Liquor ◽  
Culture Conditions ◽  
Medium Composition ◽  
Production Performance ◽  
Raw Material ◽  
Initial Ph ◽  
Fermentation Parameters ◽  
Inoculum Volume ◽  
Starch Hydrolysate

D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability. The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design. The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3. These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.

scholarly journals The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

2017 ◽  
Vol 4 (10) ◽  
pp. 171012 ◽  
Author(s):  
Miao Ye ◽  
Linghong Sun ◽  
Ru Yang ◽  
Zaigui Wang ◽  
KeZong Qi
Keyword(s):  
Incubation Time ◽  
Bacillus Amyloliquefaciens ◽  
Egg Production ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Liquid Volume ◽  
Hatching Rate ◽  
Culture Temperature ◽  
Egg Weight ◽  
Initial Ph

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg −1 ) and experiment 2 (3 g kg −1 ). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious ( p  < 0.05), and the average egg weight was significantly increased in experiment 2 ( p  < 0.05).

Optimization of Fermentation Medium and Culture Conditions of Hydrocarbon-Degrading Bacterium Brevibacillus panacihumi W25

2014 ◽  
Vol 1010-1012 ◽  
pp. 69-72
Author(s):  
Xin Xin Wang ◽  
Chen Li ◽  
Li Bin Zhao ◽  
Liang Wu ◽  
Ping Zhou ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Polynomial Regression ◽  
Ph Value ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Degrading Bacterium ◽  
Initial Ph ◽  
Optimization Of Fermentation ◽  
First Time

Members belonging to the genus Brevibacillus had potentials in the bioremediation of hydrocarbon-contaminated soils. However, little is known about the fermentation medium and culture conditions of Brevibacillus panacihumi. The effect of concentrations of glucose and (NH4)2SO4, initial pH value and culture temperature on the biomass of B. panacihumi W25 was measured with single factor experiments, which was a hydrocarbon-degrading bacterium. Polynomial regression analysis showed optimal concentration of glucose and (NH4)2SO4, initial pH value and culture temperature were 45.2 g/L, 0.6 g/L, pH 7.3 and 30.3°C, respectively. The optimization of fermentation of B. panacihumi was reported for the first time.

scholarly journals Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

2021 ◽  
Vol 9 (6) ◽  
pp. 678
Author(s):  
Kaliyamoorthy Kalidasan ◽  
Nabikhan Asmathunisha ◽  
Venugopal Gomathi ◽  
Laurent Dufossé ◽  
Kandasamy Kathiresan
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Biomass Production ◽  
Petroleum Ether Extract ◽  
Antimicrobial Activities ◽  
Culture Conditions ◽  
Gold And Silver Nanoparticles ◽  
Optimization Of Culture Conditions ◽  
Antioxidant And Antimicrobial Activities

This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

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