Characterisation and In Vitro Bioactivity of UV-Treated Anodised Titanium

2015 ◽  
Vol 1125 ◽  
pp. 450-454
Author(s):  
Te Chuan Lee ◽  
M.F.M. Rathi ◽  
M.Y.Z. Abidin ◽  
Hasan Zuhudi Abdullah ◽  
Maizlinda Izwana Idris
Keyword(s):  
Surface Properties ◽  
Uv Light ◽  
Treatment Method ◽  
Ceramic Coatings ◽  
Light Treatment ◽  
Disodium Salt ◽  
X Ray ◽  
Ceramic Films ◽  
Titanium Foils

Anodic oxidation is an electrochemical method for the production of ceramic films on a metallic substrate. It has been widely used to deposit the ceramic coatings on the metals surface. Recently, ultraviolet (UV) light treatment is gaining recognition as a new potential surface treatment method. This study aims to investigate the effect of UV light treatment on the surface properties and in vitro bioactivity of anodised titanium. At first, the titanium foils were anodised in mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). Subsequently, the anodised titanium was pre-treated with UVA lamp (peak wavelength of 365 nm) and immersed in simulated body fluid (SBF). Field emission scanning electron microscopy (FESEM), X-ray diffractometer (XRD) and goniometer were used to characterise the surface properties, crystallinity and surface wettability of untreated titanium (UT), anodised titanium (AT) and UV-treated anodised titanium (UTAT). UTAT became more hydrophilic if compared to the UAT. The result of SBF showed that bone-like apatite was precipitated on the surface of UTAT. The results indicated that hydrophilic surface is able to accelerate the growth of bone-like apatite.

Biomimetic Bone-Like Apatite Coating on Anodised Titanium in Simulated Body Fluid under UV Irradiation

2017 ◽  
Vol 888 ◽  
pp. 251-255 ◽  
Author(s):  
T.C. Lee ◽  
Pramod Koshy ◽  
Hasan Zuhudi Abdullah ◽  
M.I. Idris
Keyword(s):  
Uv Irradiation ◽  
Uv Light ◽  
Light Treatment ◽  
Disodium Salt ◽  
Apatite Formation ◽  
Oh Groups ◽  
Uva Irradiation ◽  
Apatite Coating ◽  
Titanium Foils

Low temperature deposition techniques of bioceramics coatings are now being researched and developed to avoid deficiencies inherent in high temperature techniques. Biomimetic coatings is a solution-based method conducted at ambient temperature to deposit bioactive coatings on the surface. The current study aims to investigate the effect of ultraviolet (UV) irradiation on the coating of bone-like apatite on the anodised surface. High purity titanium foils were anodised with an applied voltage of 350 V, current density of 70 mA.cm-2 in mixture of 0.04 M β-glycerophosphate disodium salt pentahydrate (β-GP) and 0.4 M calcium acetate (CA) for 10 min. After anodic oxidation, UV light treatment was conducted in pH-adjusted distilled water for 12 hours with ultraviolet light A (UVA) irradiation. Subsequently, the UV-treated anodised titanium foils were soaked in SBF for 7 days with/without UVA irradiation. After SBF immersion for 7 days, anodised titanium with combination of UV light treatment and UV irradiation during in vitro testing was fully covered by highly crystalline bone-like apatite at maximal thickness of 2.8 μm. This occurred mainly due to the formation of large amounts of Ti-OH groups which act as nucleation sites for bone-like apatite. This study also revealed that UV irradiation during in vitro testing is superior in promoting growth of bone-like apatite compared to UV light treatment. The suggested mechanism for bone-like apatite formation on anodised titanium under different UV irradiation conditions is illustrated in this article. The findings of this study indicated that biomimetic bone-like apatite coating with assistance of UV irradiation is an effective method in accelerating the formation of bone-like apatite.

Effect of UV Wavelength on Apatite Formation of Anodised Titanium

2015 ◽  
Vol 1125 ◽  
pp. 465-469
Author(s):  
Te Chuan Lee ◽  
Hasan Zuhudi Abdullah ◽  
Maizlinda Izwana Idris
Keyword(s):  
Uv Light ◽  
Chemical Properties ◽  
Light Treatment ◽  
Disodium Salt ◽  
Light Illumination ◽  
Apatite Formation ◽  
Oh Groups ◽  
Novel Method ◽  
Titanium Foils

A novel method to accelerate the apatite formation on the anodised titanium is proposed in this article. The processing was composed of two steps which were UV light treatment after anodic oxidation, and UV light illumination during soaking in simulated body fluid (SBF). This study aims to investigate the effect of different UV wavelengths during SBF on the apatite formation of anodised titanium. The titanium foils were anodised in mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). Subsequently, the anodised titanium foils were pre-treated with UV light. In vitro was conducted by illuminating with different wavelengths of UV light (254nm and 365nm) in SBF. Field emission scanning electron microscopy (FESEM) and X-ray diffractometer (XRD) were used to characterise the surface morphology and crystallinity of anodised titanium. The results showed that donut-shaped pores with anatase/rutile phases were formed on the surface of anodised titanium. Apart from that, the UV light treatment did change the chemical properties of anodised titanium by producing more •OH groups. After UV light illumination in SBF for 1 week, the anodised titanium foils were fully covered by bone-like apatite.

Effect of Current Density on Anodised Titanium in Mixture of β-Glycerophosphate (β-GP) and Calcium Acetate (CA)

2015 ◽  
Vol 1087 ◽  
pp. 212-217 ◽  
Author(s):  
Hasan Zuhudi Abdullah ◽  
Te Chuan Lee ◽  
Maizlinda Izwana Idris ◽  
Charles Christopher Sorrell
Keyword(s):  
Current Density ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Ceramic Coatings ◽  
Disodium Salt ◽  
Calcium Acetate ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ceramic Films ◽  
Glancing Angle

Anodic oxidation is an electrochemical method for the production of ceramic films on a metallic substrate. It had been widely used to deposit the ceramic coatings on the metals surface. In this study, the surface morphology and crystallinity of titanium foil was modified by anodising in mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). The experiments were carried out at high voltage (350 V), different anodising time (1, 3, 5 and 10 min) and current density (10 and 20 mA.cm-2) at room temperature. Anodised titanium was characterised by using glancing angle X-ray diffraction (GAXRD), field emission scanning electron microscope (FESEM) and focused ion beam (FIB) milling. The result of the experiment show that colour, porosity, crystallinity and thickness of the titanium films depended strongly on the current density. More porous surface and large amount of anatase was produced at higher current density. FIB results indicated that the thickness of oxide layer increased as increasing of current density.

Effect of Ultrasonic Amplitude on Surface Properties of Anodised Titanium for Biomedical Application

2016 ◽  
Vol 840 ◽  
pp. 160-164 ◽  
Author(s):  
Te Chuan Lee ◽  
Mohd Hafifi Hafizat Mazlan ◽  
Mohamad Imran Abbas ◽  
Hasan Zuhudi Abdullah ◽  
Maizlinda Izwana Idris
Keyword(s):  
Surface Properties ◽  
Biomedical Application ◽  
Low Cost ◽  
Disodium Salt ◽  
Anode Surface ◽  
Simple Method ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ceramic Films ◽  
Titanium Foils

Anodic oxidation is an electrochemical method for the production of ceramic films on a metallic substrate. It is a simple and low cost method to produce bioactive material. This work describes the effect of ultrasonic amplitude on the surface properties of anodised titanium. Specifically, high purity titanium foils were anodised in mixture of 0.04 M β-glycerophosphate disodium salt pentahydrate (β-GP) and 0.4 M calcium acetate monohydrate (CA) at 350 V and 70 mA.cm-2 for 10 minutes. The ultrasonic amplitude was varied from 20-60 μm. Next, field emission scanning electron microscopy (FESEM) glancing angle X-ray diffractometer (GAXRD) and atomic force microscopy (AFM) were used to characterise the anodised titanium. The results showed that application of sonication is able to remove the entrapped bubbles on the anode surface and enhance the oxidation process. The pores size and surface roughness were increased as increasing of ultrasonic amplitude. At ultrasonic amplitude ≥ 50 μm, rutile TiO2 was formed on the surface of oxide layer. It was found that the sonication is a simple method to improve the surface properties of anodised titanium for implant applications.

Effect of UV Light Treatment Condition on Apatite Formation of Anodised Titanium

2015 ◽  
Vol 1125 ◽  
pp. 460-464
Author(s):  
Te Chuan Lee ◽  
Maizlinda Izwana Idris ◽  
Hasan Zuhudi Abdullah
Keyword(s):  
Treatment Condition ◽  
Uv Light ◽  
Light Treatment ◽  
Disodium Salt ◽  
Alkaline Condition ◽  
Light Illumination ◽  
Apatite Formation ◽  
Water Ph ◽  
Uv Lamp ◽  
Titanium Foils

A novel method to accelerate the apatite formation on the anodised titanium is proposed in this article. The processing was composed of two steps which were UV light treatment after anodic oxidation, and UV light illumination during simulated body fluid (SBF). This study aims to investigate the effect of UV light treatment condition on the apatite formation of anodised titanium. The titanium foils were anodised in mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). UV light treatment was conducted in pH-adjusted distilled water (pH 1, 4, 7 and 11) for 12 hours. Next, SBF was carried out by illuminating with UV lamp for 1 week. Anodised titanium foils were characterised by using field emission scanning electron microscopy (FESEM), X-ray diffractometer (XRD) and goniometer. The results showed that donut-shaped were formed on the surface of anodised titanium. The surface of anodised titanium became more hydrophilic after UV-treated in acidic and alkaline condition. After UV light illumination in SBF for 1 week, the anodised titanium foils which UV-treated in acidic condition presented more crystalline bone-like apatite on the surface.

Effect of Bath Temperature on Surface Properties of Anodised Titanium for Biomedical Application

2016 ◽  
Vol 840 ◽  
pp. 175-179 ◽  
Author(s):  
Te Chuan Lee ◽  
Mohd Hafifi Hafizat Mazlan ◽  
Mohamad Imran Abbas ◽  
Hasan Zuhudi Abdullah ◽  
Maizlinda Izwana Idris
Keyword(s):  
Surface Properties ◽  
Biomedical Application ◽  
Bath Temperature ◽  
Ceramic Coatings ◽  
Disodium Salt ◽  
Calcium Acetate ◽  
Titanium Foil ◽  
Acetate Monohydrate ◽  
X Ray ◽  
Glancing Angle

Anodic oxidation is an electrochemical method to deposit ceramic coatings on the metals substrate to improve the bioactivity. This study aims to investigate the effect of bath temperature on the surface properties of anodised titanium. High-purity titanium foil was modified by anodising in mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). The experiments were carried out at 350 V, 30 mA.cm-2 for 10 minutes at different bath temperature (4-100 °C). Field emission scanning electron microscopy (FESEM), glancing angle X-ray diffractometer (GAXRD) and goniometer were used to characterise the surface morphology, mineralogy and wettability of anodised titanium, respectively. The results showed that porosity and crystallinity of surface decreased as increasing of bath temperature. Interestedly, the α-tricalcium phosphate (α-TCP) was deposited on the samples which anodisation at higher bath temperature (≥ 60 °C) and resulted high hydrophilicity behaviour even the surface was found relatively smooth.

CONTROL OF SURFACE MODIFIED LAYER ON METALLIC BIOMATERIALS BY AN ADVANCED ELID GRINDING SYSTEM (EG-X)

2006 ◽  
Vol 20 (25n27) ◽  
pp. 3605-3610 ◽  
Author(s):  
MASAYOSHI MIZUTANI ◽  
JUN KOMOTORI ◽  
KAZUTOSHI KATAHIRA ◽  
HITOSHI OHMORI
Keyword(s):  
Oxide Layer ◽  
Surface Properties ◽  
Photoelectron Spectroscopy ◽  
Titanium Implants ◽  
X Ray ◽  
Elid Grinding ◽  
Modified Layer ◽  
Surface Modified ◽  
Grinding System

The biocompatibility of titanium implants with different surface properties is investigated. We prepared three types of specimens, one ground by the newly developed ELID grinding system, another ground by conventional ELID grinding, and the other polished by SiO 2 powder. These surfaces were characterized and, the number of cell and cytotoxicity in in-vitro were measured. Energy Dispersive X-ray Spectroscopy (EDS), X-ray Photoelectron Spectroscopy (XPS) and Transmission Electron Microscope (TEM) revealed that the modified ELID system can create a significantly thick oxide layer and a diffused oxide layer, and also can control the thickness of a modified layer. The results of cell number and cytotoxicity showed that the sample ground by the modified system had the highest biocompatibility. This may have been caused by improvement of chemical properties due to a surface modified layer. The above results suggest that this newly developed ELID grinding system can create the desirable surface properties. Consequently, this system appears to offer significant future promise for use in biomaterials and other engineering components.

Improved in vitro quality of stored red blood cells upon oxygen reduction prior to riboflavin/UV light treatment of whole blood

Transfusion ◽  
2019 ◽  
Vol 59 (10) ◽  
pp. 3197-3204
Author(s):  
Peter Schubert ◽  
Brankica Culibrk ◽  
Deborah Chen ◽  
Katherine Serrano ◽  
Elena Levin ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Oxygen Reduction ◽  
Whole Blood ◽  
Blood Cells ◽  
Uv Light ◽  
Light Treatment

scholarly journals In Vitro Properties of Manganese-Substituted Tricalcium Phosphate Coatings for Titanium Biomedical Implants Deposited by Arc Plasma

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4411 ◽  
Author(s):  
Inna V. Fadeeva ◽  
Vasilii I. Kalita ◽  
Dmitry I. Komlev ◽  
Alexei A. Radiuk ◽  
Alexander S. Fomin ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Plasma Deposition ◽  
L929 Cell ◽  
Octacalcium Phosphate ◽  
Ceramic Coatings ◽  
Physiological Solution ◽  
Human Tooth ◽  
Arc Plasma ◽  
X Ray

Bioactive manganese (Mn)-doped ceramic coatings for intraosseous titanium (Ti) implants are developed. Arc plasma deposition procedure is used for coatings preparation. X-ray Diffraction, Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy, and Electron Paramagnetic Resonance (EPR) methods are applied for coatings characterization. The coatings are homogeneous, composed of the main phase α-tricalcium phosphate (α-TCP) (about 67%) and the minor phase hydroxyapatite (about 33%), and the Mn content is 2.3 wt%. EPR spectroscopy demonstrates that the Mn ions are incorporated in the TCP structure and are present in the coating in Mn2+ and Mn3+ oxidation states, being aggregated in clusters. The wetting contact angle of the deposited coatings is suitable for cells’ adhesion and proliferation. In vitro soaking in physiological solution for 90 days leads to a drastic change in phase composition; the transformation into calcium carbonate and octacalcium phosphate takes place, and no more Mn is present. The absence of antibacterial activity against Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa bacteria strains is observed. A study of the metabolic activity of mouse fibroblasts of the NCTC L929 cell line on the coatings using the MTT (dye compound 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test demonstrates that there is no toxic effect on the cell culture. Moreover, the coating material supports the adhesion and proliferation of the cells. A good adhesion, spreading, and proliferative activity of the human tooth postnatal dental pulp stem cells (DPSC) is demonstrated. The developed coatings are promising for implant application in orthopedics and dentistry.

scholarly journals Copper-Chitosan Nanocomposite Hydrogels Against Aflatoxigenic Aspergillus flavus from Dairy Cattle Feed

2020 ◽  
Vol 6 (3) ◽  
pp. 112
Author(s):  
Kamel A. Abd-Elsalam ◽  
Mousa A. Alghuthaymi ◽  
Ashwag Shami ◽  
Margarita S. Rubina ◽  
Sergey S. Abramchuk ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antifungal Activity ◽  
Aspergillus Flavus ◽  
Uv Light ◽  
Peanut Meal ◽  
High Concentration ◽  
Fungal Cell ◽  
X Ray ◽  
Toxigenic Fungi

The integration of copper nanoparticles as antifungal agents in polymeric matrices to produce copper polymer nanocomposites has shown excellent results in preventing the growth of a wide variety of toxigenic fungi. Copper-chitosan nanocomposite-based chitosan hydrogels (Cu-Chit/NCs hydrogel) were prepared using a metal vapor synthesis (MVS) and the resulting samples were described by transmission electron microscopy (TEM), X-ray fluorescence analysis (XRF), and small-angle X-ray scattering (SAXS). Aflatoxin-producing medium and VICAM aflatoxins tests were applied to evaluate their ability to produce aflatoxins through various strains of Aspergillus flavus associated with peanut meal and cotton seeds. Aflatoxin production capacity in four fungal media outlets revealed that 13 tested isolates were capable of producing both aflatoxin B1 and B2. Only 2 A. flavus isolates (Af11 and Af 20) fluoresced under UV light in the A. flavus and parasiticus Agar (AFPA) medium. PCR was completed using two specific primers targeting aflP and aflA genes involved in the synthetic track of aflatoxin. Nevertheless, the existence of aflP and aflA genes indicated some correlation with the development of aflatoxin. A unique DNA fragment of the expected 236 bp and 412 bp bands for aflP and aflA genes in A. flavus isolates, although non-PCR fragments have been observed in many other Aspergillus species. This study shows the antifungal activity of Cu-Chit/NCs hydrogels against aflatoxigenic strains of A. flavus. Our results reveal that the antifungal activity of nanocomposites in vitro can be effective depending on the type of fungal strain and nanocomposite concentration. SDS-PAGE and native proteins explain the apparent response of cellular proteins in the presence of Cu-Chit/NCs hydrogels. A. flavus treated with a high concentration of Cu-Chit/NCs hydrogels that can decrease or produce certain types of proteins. Cu-Chit/NCs hydrogel decreases the effect of G6DP isozyme while not affecting the activity of peroxidase isozymes in tested isolates. Additionally, microscopic measurements of scanning electron microscopy (SEM) showed damage to the fungal cell membranes. Cu-Chit/NCS hydrogel is an innovative nano-biopesticide produced by MVS is employed in food and feed to induce plant defense against toxigenic fungi.

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