Determination Method and Extraction Process of Total Flavonoids from Leaves of Ilex Cornuta L.

2011 ◽  
Vol 236-238 ◽  
pp. 216-219
Author(s):  
Chun Tao Kuang ◽  
Xiang Zhou Li ◽  
Yan Li Han ◽  
Yong Jun Wu
Keyword(s):  
Regression Coefficient ◽  
Orthogonal Design ◽  
Extraction Process ◽  
Total Flavonoids ◽  
Determination Method ◽  
Relative Standard ◽  
Ilex Cornuta ◽  
Total Flavonoids Content ◽  
Solid Liquid ◽  
Important Medicinal Plant

Ilex Cornuta L is an important medicinal plant. Flavonoid compounds are its main biological compontents. AlCl3 method was identified as the appropriate determination method for total flavonoids content of extract obtained from the leaves of Ilex Cornuta L.. The proposed method was validated by the linearity, reproducibility, and recovery experiments. Good linearity was observed with the regression coefficient(R2= 0.999). The recovery rate was in the range of 94.9% and 110.0%, and the relative standard deviation was 2.8%, which indicated that the proposed method was credible. In addition, the extraction parameters including Ethanol concentration, solid-liquid ratio, extraction time and extraction times were optimized by means of an orthogonal design.

scholarly journals Optimization of Supercritical Extraction Conditions of Senna Alata and Evaluation of Biological Activity

2018 ◽  
Vol 7 (3.11) ◽  
pp. 94
Author(s):  
Istikamah Subuki ◽  
Aiman Nabilah Abdul Malek ◽  
Saidatul Husni Saidin ◽  
Mazura Md. Pisar
Keyword(s):  
Crude Extract ◽  
Extraction Process ◽  
Quadratic Model ◽  
Total Flavonoids ◽  
Inhibition Activity ◽  
Backward Elimination ◽  
Total Flavonoids Content ◽  
Senna Alata ◽  
Central Composite ◽  
Experimental Runs

Supercritical fluid extraction (SFE) offer faster extraction process, decreased solvent usage and more selectivity on desired compounds. In this present study, the influence of pressure (100, 200 and 300 bar) and temperature (40, 50 and 60˚C) on the Senna alata crude yield were investigated with fixed supercritical carbon dioxide (SC-CO2) at the flow rate of 35 g/min. The parameters were optimised and modelled using response surface methodology (RSM) and central composite design (CCD). The analysis of variance (ANOVA) experimental design consists of 13 experimental runs with 5 replicates at the central points. Well-fitting quadratic model were successfully established for crude extract through backward elimination. The optimum crude extract yield pointed out by RSM was at the pressure of 300 bar and temperature 40˚C respectively. Extraction yields based on SC-CO2 varied in the range of 0.28 to 3.62%. The highest hyaluronidase inhibition activity and total flavonoids content obtained by S.alata crude extracts were 41.19% and 52.53% w/w, respectively. SC-CO2 proves to have great potential for extraction of yield, hyaluronidase inhibition activity and total flavonoids content for S.alata.  

scholarly journals Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Bambang Kunarto ◽  
Ely Yuniarti Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Extraction Process ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain  bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

Study on Extraction of Total Flavonoids from Licorice with Ultrasonic Assistance and Antioxidant Activity

2012 ◽  
Vol 550-553 ◽  
pp. 1629-1632 ◽  
Author(s):  
Yu Hong Zou ◽  
Qiu Xia Han ◽  
Lin Guo
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Process ◽  
Free Radical Scavenger ◽  
Extraction Rate ◽  
Radical Scavenger ◽  
Total Flavonoids ◽  
Ultrasonic Power ◽  
Liquid Ratio ◽  
Solid Liquid

To optimize the extraction process of total flavonoids from RADIX RHIZOMA GLYCYRRHIZAE and Study on the Antioxidant Activity. Total flavonoids were extracted from RADIX RHIZOMA GLYCYRRHIZAE with ethanol by ultrasonic-assisted method, whose extraction process was optimized by orthogonal test design.The scavenging effect of pumpkin polysaccharide on OH and O2-were studied by salicylic acid and pyrogallic acid method. The optimum extraction process was as follows: ethanol concentration of 75%, solid-liquid ratio of 1:25,ultrasonic power of 350 W and ultrasonic duration of 25 min.Under the above conditions, the extraction rate of total flavonoids reached as high as 1.943%. The influencing order of each factor on extraction rate of polysaccharides was determined to be: ethanol concentration＞solid-liquid ratio＞ultrasonic power＞ultrasonic duration. When the solution concentration is 1.1296mg/ml, the scavenging rate of crude flavonoids on superoxide radical is 50%.Suggesting that it would be an effctive natural free radical scavenger. The study could provide a scientific basis for the further exploitation and utilization of RADIX RHIZOMA GLYCYRRHIZAE

scholarly journals Optimization of Rose Extraction Process by Orthogonal Design

2021 ◽  
pp. 498-515
Author(s):  
Xin Zhang, Dan Chen, Xiao Cao, Heng Li, Limin Wu
Keyword(s):  
Antioxidant Capacity ◽  
Extraction Method ◽  
Ethanol Concentration ◽  
Orthogonal Design ◽  
Water Extract ◽  
Extraction Process ◽  
Utilization Rate ◽  
Extraction Scheme ◽  
Solid Liquid ◽  
The Rose

To optimize the rose extraction process and evaluate the antioxidant properties of the extracts. The contents of gallic acid and rutin in rose extracts were used as indicators. According to the results of single-factor evaluation of the crushing degree of rose during extraction, extraction method, solid-liquid ratio of extraction and ethanol concentration. The three-factor and three-level L9 (33) orthogonal design optimized extraction scheme was established. The factor levels were as follows: solid-liquid ratio (1:8, 1:10, 1:12); Ethanol concentration of extractant (50%, 80%, 100%); Extraction method (ultrasonic treatment with ice for 30min, 3 times; Ultrasonic treatment with ice for 1h and 3 times; Reflux in water bath for 1h, 3 times); The DPPH in vitro antioxidant test was applied to compare the antioxidant capacity of the optimized extract and rose water extract. The best extraction scheme of rose was as follows: the ratio of solid to liquid was 1:10, the concentration of ethanol was 80%, and the extraction method was ultrasonic for 1 hour and repeated three times. In the antioxidant experiment, it can be found that the antioxidant capacity of the extract is significantly higher than that of the rose water extract. The optimized rose extract has a significantly higher antioxidant capacity than the rose water extract. The establishment of the extraction method significantly improves the utilization rate of antioxidant components in roses. It not only helps to improve the utilization rate of roses and avoids waste, but also helps the development and utilization of rose-related health products.

scholarly journals Optimization of extraction and quantification of Flavonoids from Averrhoa bilimbi fruits using RP-HPLC and its correlation between total flavonoids content against antimicrobial activity

2021 ◽  
Author(s):  
Tan Phat Chau ◽  
Muthusamy Muthusamy ◽  
Arunachalam Chinnathambi ◽  
Tahani Awad Alahmadi ◽  
Sowmya Kuppusamy
Keyword(s):  
Antimicrobial Activity ◽  
Total Flavonoids ◽  
Rp Hplc ◽  
Total Flavonoids Content

scholarly journals High-efficiency method for recycling lithium from spent LiFePO4 cathode

2020 ◽  
Vol 9 (1) ◽  
pp. 1586-1593
Author(s):  
Tingting Yan ◽  
Shengwen Zhong ◽  
Miaomiao Zhou ◽  
Xiaoming Guo ◽  
Jingwei Hu ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
High Efficiency ◽  
Optical Emission ◽  
Extraction Process ◽  
Emission Spectrometry ◽  
X Ray Diffraction ◽  
Inductively Coupled ◽  
Moisture Analysis ◽  
Lifepo4 Cathode ◽  
Solid Liquid

Abstract The extraction of Li from the spent LiFePO4 cathode is enhanced by the selective removal using interactions between HCl and NaClO to dissolve the Li+ ion while Fe and P are retained in the structure. Several parameters, including the effects of dosage and drop acceleration of HCl and NaClO, reaction time, reaction temperature, and solid–liquid ratio on lithium leaching, were tested. The Total yields of lithium can achieve 97% after extraction process that lithium is extracted from the precipitated mother liquor, using an appropriate extraction agent that is a mixture of P507 and TBP and NF. The method also significantly reduced the use of acid and alkali, and the economic benefit of recycling is improved. Changes in composition, morphology, and structure of the material in the dissolution process are characterized by inductively coupled plasma optical emission spectrometry, scanning electron microscope, X-ray diffraction, particle size distribution instrument, and moisture analysis.

Mathematical Modeling of Betanin Extraction from Red Beet (Beta vulgaris L.) by Solid–Liquid Method

2015 ◽  
Vol 11 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Honggao Xu ◽  
Qiang Peng ◽  
Fang Yuan ◽  
Yanxiang Gao
Keyword(s):  
Mathematical Modeling ◽  
Effective Diffusion ◽  
Spherical Model ◽  
Extraction Process ◽  
Extraction Temperature ◽  
Optimal Parameters ◽  
Practical Application ◽  
Sulfurous Acid ◽  
Red Beet ◽  
Solid Liquid

Abstract Effective diffusion coefficient (D-value) is an important parameter for the extraction process. In this study, a cylindrical model to estimate the D-value of betanin extraction from red beet roots was developed. The influence of extraction temperature, pH, cylindrical thickness and type of acids on the D-value was also investigated. The optimal parameters of betanin extraction were 30°C, pH 4.0 adjusted with sulfurous acid or acetic acid. D-values under the above conditions were 18.95×10−11 m2/s and 17.87×10−11 m2/s, respectively. The result showed that D-value elevated with the increase of cylindrical thickness, which was consistent with earlier conclusion from spherical model. The modeling may be useful for the investigation into extraction process and practical application.

scholarly journals Quantitative Determination of Azithromycin in Human Plasma by Liquid Chromatography–Mass Spectrometry and its Application in Pharmackokinetic Study

2012 ◽  
Vol 11 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Maizbha Uddin Ahmed ◽  
Mohammad Safiqul Islam ◽  
Tasmin Ara Sultana ◽  
AGM Mostofa ◽  
Muhammad Shahdaat Bin Sayeed ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Solid Phase ◽  
Internal Standard ◽  
Extraction Process ◽  
Serum Samples ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Linear Concentration Range ◽  
Linear Concentration

Azithromycin is an effective and well-known antimicrobial agent. In the present study, a simple, sensitive and specific LC/MS/MS method has been developed and validated for the quantification of Azithromycin in  human serum samples using Clarithromycin as internal standard. Azithromycin was extracted from biological matrix  by using solid phase extraction process. The chromatographic separation was performed on Luna C18 (3 ?, 2x150   mm) column with a mobile phase consisting of 35 mM ammonium acetate buffer (mobile phase-A) and acetonitrile  and methanol in ratio of 90:10 ( as mobile phase-B) at a flow rate of 0.25 mL/min. The method was validated over a  linear concentration range of 0.5?50.0 ng/mL and limit of quantification (LOQ) was 0.5 ng/mL with a coefficient of  correlation (r2) = 0.9998. The intra-day and inter-day precision expressed as relative standard deviation were 1.64% – 8.43% and 2.32% – 9.92%, respectively. The average recovery of azithromycin from serum was 98.11%. The method  was successfully applied to a pharmacokinetic study after oral administration of Azithromycin 200 mg/5 ml suspension in healthy Bangladeshi volunteers. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12488 Dhaka Univ. J. Pharm. Sci. 11(1): 55-63, 2012 (June)

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