Fabrication and Characterization of Core-Shell Structured Composite Nanofibrous Scaffolds by Coaxial Electrospinning

2011 ◽  
Vol 393-395 ◽  
pp. 754-757
Author(s):  
Qing Ye ◽  
Kui Hua Zhang ◽  
Sheng Bao Ke ◽  
Chao Liang Wang ◽  
Hong Yi Zhang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Mechanical Test ◽  
Ethanol Vapor ◽  
Random Coil ◽  
Coaxial Electrospinning ◽  
Core Shell ◽  
Nanofibrous Scaffolds ◽  
Transmission Electron ◽  
Β Sheet

Biodegradable core-shell structured nanofibrous scaffolds with silk fibroin (SF) and poly(L-lactic acid-co-ε-caprolactone) (P(LLA-CL)) blends as shell and bovine serum albumin (BSA)-containing SF as core were fabricated by coaxial electrospinning. Morphology and microstructure of the nanofibers were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results demonstrated that core-shell structured nanofibers were obtained, furthermore, the nanofibers became greatly uniform and the average diameters were thinner in comparison with only core BSA solution after adding 10-20 w/v% SF in core BSA solution. The 13C NMR clarified the SF conformation transformed from random coil to β-sheet when treated with 75 w/v % ethanol vapor. The mechanical test showed core-shell structured nanofibrous scaffolds possessed good mechanical properties but lower than SF/P(LLA-CL) blended nanofibrous scaffolds.

Influence of Post-Treatment with Methanol Vapor on the Properties of SF/P(LLA-CL) Nanofibrous Scaffolds

2011 ◽  
Vol 236-238 ◽  
pp. 2221-2224
Author(s):  
Kui Hua Zhang ◽  
Xiu Mei Mo
Keyword(s):  
Electrospun Nanofibers ◽  
Random Coil ◽  
Methanol Vapor ◽  
Nanofibrous Scaffolds ◽  
Nanofibrous Structure ◽  
Α Helix ◽  
Β Sheet ◽  
Ideal Method ◽  
C Nmr

In order to improve water-resistant ability silk fibroin (SF) and SF/P(LLA-CL) blended nanofibrous scaffolds for tissue engineering applications, methanol vapor were used to treat electrospun nanofibers. SEM indicated SF and SF/ P(LLA-CL) scaffolds maintained nanofibrous structure after treated with methanol vapor and possessed good water-resistant ability. Characterization of 13C NMR clarified methanol vapor induced SF conformation from random coil or α- helix to β-sheet. Moreover, treated SF/ P (LLA-CL) nanofibrous scaffolds still kept good mechanical properties. Methanol vapor could be ideal method to treat SF and SF/ P(LLA-CL) nanofibrous scaffolds for biomedical applications.

Fabrication and Characterization of Vitamin E TPGS Loaded Silk Fibroin/Hyaluronic Acid Nanofibrous Scaffolds

2013 ◽  
Vol 721 ◽  
pp. 274-277
Author(s):  
Li Li Ji ◽  
Qiao Ling Li ◽  
Zeng Hu Yang ◽  
Wei Jing Hu ◽  
Kui Hua Zhang
Keyword(s):  
Hyaluronic Acid ◽  
Vitamin E ◽  
Silk Fibroin ◽  
Ethanol Vapor ◽  
Random Coil ◽  
Burst Release ◽  
Nanofibrous Scaffolds ◽  
Vitamin E Tpgs ◽  
Polyethylene Glycol 1000 ◽  
Β Sheet

Vitamin E d-alpha-tocopheryl polyethylene glycol 1000 succinate (VE TPGS) loaded silk fibroin (SF)/ hyaluronic acid (HA) nanofibrous scaffolds were fabricated by means of electrospinning to biomimic the natural extracellular matrix. Scanning electronic microscopy (SEM) results indicated that electrospun VE TPGS loaded SF/HA nanofibers were ribbon-shaped, the width of nanofibers decreased slightly with the addition of VE TPGS to SF/HA blended solutions. Fourier transform infrared (FTIR) spectroscopy and Wide-angle X-ray diffraction (WAXD) curves revealed that VE TPGS did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. In vitro release studies confirmed VE TPGS had no obvious burst release and present good release behavior.

scholarly journals Core-shell nanofibers as drug delivery systems

2019 ◽  
Vol 69 (2) ◽  
pp. 131-153 ◽  
Author(s):  
Špela Zupančič
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery Systems ◽  
Protein Gene ◽  
Delivery Systems ◽  
Critical Parameters ◽  
Core Shell ◽  
Immiscible Polymer Blends ◽  
Transmission Electron ◽  
Structure And Activity

Abstract Core-shell nanofibers have grown in popularity over the last decade owing to their special features and their many applications in biomedicine. They can be produced by electrospinning of immiscible polymer blends or emulsions through a single nozzle or by electrospinning using a coaxial nozzle. Several of the electrospinning parameters allow great versatility for the compositions and diameters of core-shell nanofibers to be produced. Morphology of core-shell nanofibers can be investigated using transmission electron microscopy and, in some cases, scanning electron microscopy. Several studies have shown that core-shell nanofibers have some advantages over monolithic nanofibers, such as better drug, protein, gene or probiotic incorporation into the nanofibers, greater control over drug release, and maintenance of protein structure and activity during electrospinning. We herein review the production and characterization of core-shell nanofibers, the critical parameters that affect their development, and their advantages as delivery systems.

scholarly journals Synthesis and Characterization of Conducting PANDB/χ-Al2O3 Core-Shell Nanocomposites by In Situ Polymerization

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2787
Author(s):  
Cheng-Ho Chen ◽  
Ying-Chen Lin ◽  
Fu-Su Yen
Keyword(s):  
Electron Microscopy ◽  
In Situ Polymerization ◽  
Weight Ratio ◽  
Core Shell ◽  
Sem Images ◽  
Dodecylbenzenesulfonic Acid ◽  
Blend Film ◽  
Transmission Electron

Polyaniline doped with dodecylbenzenesulfonic acid/χ-aluminum oxide (PANDB/χ-Al2O3) conducting core-shell nanocomposites was synthesized via an in situ polymerization method in this study. PANDB was synthesized in the presence of dodecylbenzenesulfonic acid (DBSA), which functioned as a dopant and surfactant. The electrical conductivity of the conducting PANDB/χ-Al2O3 core-shell nanocomposite was approximately 1.7 × 10−1 S/cm when the aniline/χ-Al2O3 (AN/χ-Al2O3) weight ratio was 1.5. The transmission electron microscopy (TEM) results indicated that the χ-Al2O3 nanoflakes were thoroughly coated by PANDB to form the core-shell (χ-Al2O3-PANDB) structure. The TEM and field-emission scanning electron microscopy (FE-SEM) images of the conducting PANDB/χ-Al2O3 core-shell nanocomposites also indicated that the thickness of the PANDB layer (shell) could be increased as the weight ratio of AN/χ-Al2O3 was increased. In this study, the optimum weight ratio of AN/χ-Al2O3 was identified as 1.5. The conducting PANDB/χ-Al2O3 core-shell nanocomposite was then blended with water-based polyurethane (WPU) to form a conducting WPU/PANDB/χ-Al2O3 blend film. The resulting blend film has promising antistatic and electrostatic discharge (ESD) properties.

Preparation and Characterization of Platinum/Polyaniline Core-Shell Composite

2011 ◽  
Vol 347-353 ◽  
pp. 3302-3305
Author(s):  
Jing Na Zhu ◽  
Zhen Lu Shen ◽  
Wei Min Mo ◽  
Mei Chao Li
Keyword(s):  
Electron Microscopy ◽  
Interfacial Polymerization ◽  
Core Shell ◽  
The Core ◽  
Transmission Electron ◽  
Shell Composite ◽  
Synthesis Approach ◽  
Granular Morphology ◽  
Platinum Salt

Abstract. The core-shell composite of platinum/polyaniline (Pt@PAN) had been prepared by chemical synthesis approach. Reduction of the platinum salt in aqueous solution leaded to the formation of platinum nanoparticles, and then polyaniline were synthesized by interfacial polymerization to get Pt@PAN composite. Transmission electron microscopy of Pt@PAN showed Pt particles are uniform with spherical and granular morphology. Pt@PAN was also characterized by EDAX, XPS and FTIR.

Synthesis and Characterization of the Gold-SiO2 Core-Shell Nanoparticle on the X-Nanozeoliate Used for Immobilization of the Alkaline Protease Enzyme

2012 ◽  
Vol 326-328 ◽  
pp. 93-98
Author(s):  
Nazanin Farhadyar ◽  
Mirabdullah Seyed Sadjadi
Keyword(s):  
Electron Microscopy ◽  
Alkaline Protease ◽  
Immobilized Enzymes ◽  
Core Shell ◽  
Liquid Form ◽  
Protease Enzyme ◽  
Transmission Electron ◽  
Type Zeolite ◽  
Activity Of Enzymes

Immobilized enzymes enhance process robustness, allow longer duration of activity of enzymes, and re-use of the same enzymes in multiple cycles. Enzymes can be operated in the liquid form or immobilized on various supports. In this work, we prepared gold nanoparticle core-shell structure by assembling of the gold nanoparticles on the surface of amine-functionalized x-type zeolite and then used them for immobilization of the alkaline protease. Characterization of these assembled systems were carried out by UVvisible, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy dispersive analysis of X-ray (EDAX). Biocatalytic activity of the alkaline protease in this bioconjugate system was examined and showed an increase in comparing with the free enzyme in solution.

scholarly journals Doxycycline-Eluting Core-Shell Type Nanofiber-Covered Trachea Stent for Inhibition of Cellular Metalloproteinase and Its Related Fibrotic Stenosis

Pharmaceutics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 421 ◽  
Author(s):  
Baskaran ◽  
Ko ◽  
Davaa ◽  
Park ◽  
Jiang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Drug Loading ◽  
In Vitro Release ◽  
Coaxial Electrospinning ◽  
Core Shell ◽  
Transmission Electron ◽  
Electrospinning Method ◽  
Shell Forming ◽  
Tissue Models

In this study, we fabricated a doxycycline (doxy)-eluting nanofiber-covered endotracheal stent for the prevention of stent intubation-related tissue fibrosis and re-stenosis. The nanofiber was deposited directly on the outer surface of the stent using a coaxial electrospinning method to form a doxy-eluting cover sleeve. Poly(d,l-lactide) was used as the shell-forming polymer and dedicated drug release-control membrane. Polyurethane was selected as the drug-loading core polymer. The compositional ratio of the core to shell was adjusted to 1:0, 1:2, and 1:4 by changing the electro-spray rate of each polymeric solution and microscopic observation of nanofibers using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the fluorescence microscopy proved core-shell structure of nanofibers. The in vitro release study suggested that the release of doxy could be controlled by increasing the compositional ratio of the shell. The growth of HT1080 fibrosarcoma cells was inhibited by the 10% doxy-containing nanofiber. The real-time polymerase chain reaction (PCR) in HT1080 cells and xenografted tissue models indicated that the doxy-releasing nanofiber inhibited mRNA expression of metalloproteinases (MT1-MMP, MMP-2, and MMP-9). Overall, our study demonstrates that a doxy-eluting core-shell nanofiber stent can be successfully fabricated using coaxial electrospinning and displays the potential to prevent fibrotic re-stenosis, which is the most problematic clinical complication of tracheal stent intubation.

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