Preparation of Paclitaxel-Loaded Microspheres with Fe3O4 Nanoparticles and Study of their Target-Orientation and Sustained-Release Property

2012 ◽  
Vol 535-537 ◽  
pp. 521-524
Author(s):  
Sheng Cui ◽  
Ben Lan Lin ◽  
Xiao Dong Shen
Keyword(s):  
Sustained Release ◽  
Loading Rate ◽  
Drug Loading ◽  
Target Orientation ◽  
Mtt Method ◽  
Transmission Electron ◽  
Release Property ◽  
Mean Size ◽  
Magnetic Fe3o4 Nanoparticles ◽  
The Mean

Paclitaxel-loaded microspheres with magnetic Fe3O4 nanoparticles were synthesized with albumin as carrier. The morphology was characterized by high-resolution transmission electron microscopy (HRTEM). The drug-loading rate and entrapment rate of the microspheres were researched by ultraviolet and visible spectrophotometer. The inhibition action of taxol microspheres to growth of esophagus cancer Eca109 cell were examined by MTT method. The result shows that it is regular spherical. The mean size and size distribution of microspheres are 275.6nm and 144.3nm by Zetasizer3000. The drug-loading rate is 3.013% and the entrapment rate is 35.26%. It has excellent target-orientation and sustained-release property which can prevent the growth of Eca109 cell.

scholarly journals MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Haobo Huang ◽  
Jinfeng Zhu ◽  
Liping Fan ◽  
Qiuyan Lin ◽  
Danhui Fu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Bioinformatic Analysis ◽  
Mirna Microarray ◽  
Qrt Pcr ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean ◽  
Polymerase Chain

Purpose. To elucidate the microRNAs existent in exosomes derived from stored red blood cell (RBC) unit and their potential function. Materials and Methods. Exosomes were isolated from the supernatant derived from stored RBC units by sequential centrifugation. Isolated exosomes were characterized by TEM (transmission electron microscopy), western blotting, and DLS (dynamic light scattering). MicroRNA (miRNA) microarray was performed to detect the expression of miRNAs in 3 exosome samples. Results revealed miRNAs that were simultaneously expressed in the 3 exosome samples and were previously reported to exist in mature RBCs. Functions and potential pathways of some detected miRNAs were illustrated by bioinformatic analysis. Validation of the top 3 abundant miRNAs was carried out by qRT-PCR (quantitative reverse transcription‐polymerase chain reaction). Results. TEM and DLS revealed the mean size of the exosomes (RBC-derived) as 64.08 nm. These exosomes exhibited higher abundance of short RNA than the long RNA. 78 miRNAs were simultaneously detected in 3 exosome samples and mature RBCs. Several biological processes might be impacted by these miRNAs, through their target gene(s) enriched in a particular signalling pathway. The top 3 (abundant) miRNAs detected were as follows: miR-125b-5p, miR-4454, and miR-451a. qRT-PCR revealed higher abundance of miR-451a than others. Only miR-4454 and miR-451a abundance tended to increase with increasing storage time. Conclusion. Exosomes derived from stored RBC units possessed multiple miRNAs and, hence, could serve various functions. The function of exosomes (RBC-derived) might be implemented partly by the predominantly enriched miR-451a.

Surface Modification and Preparation of High Purity Nanometer Magnesium Hydroxide Using Impinging Stream

2011 ◽  
Vol 675-677 ◽  
pp. 847-851
Author(s):  
Jian Hai Zhao ◽  
Yan Ping Lai
Keyword(s):  
Surface Modification ◽  
Magnesium Hydroxide ◽  
High Purity ◽  
X Ray Diffraction ◽  
Control Factors ◽  
Operation Conditions ◽  
Crystallization Method ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean

High purity nanometer magnesium hydroxide is produced by impinging stream reaction crystallization method using bischofite as feedstock. Effects of operation conditions on the impinging stream of Mg (OH)2 are reported and the control factors of purity are confirmed. The morphology of the powder was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Result shows that purity of Mg (OH)2 can reach 99% and the mean size of crystal is 13.5nm. Surface modification of nano-sized magnesium hydroxide using four surface modifiers such as sodium steatite, sodium laurylsulfonate, sodium oleate and sodium silicate were investigated in this paper. The modified magnesium hydroxide has smaller particle size, larger powder contact angle, slower sedimentation velocity, the less in-oil capacity than unmodified sample

scholarly journals Curcumin-Loaded Mixed Micelles: Preparation, Characterization, and In Vitro Antitumor Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Suping Ji ◽  
Xiao Lin ◽  
Enjiang Yu ◽  
Chengyang Dian ◽  
Xiong Yan ◽  
...  
Keyword(s):  
Mixed Micelles ◽  
Drug Loading ◽  
Molecular State ◽  
X Ray Diffraction ◽  
X Ray ◽  
Release Property ◽  
Mean Size ◽  
Xrd Patterns ◽  
Mcf 7

The objective of this study was to prepare curcumin-loaded mixed Soluplus/TPGS micelles (Cur-TPGS-PMs) for oral administration. The Cur-TPGS-PMs showed a mean size of 65.54 ± 2.57 nm, drug encapsulation efficiency over 85%, and drug loading of 8.17%. The Cur-TPGS-PMs were found to be stable in various pH media (pH 1.2 for 2 h, pH 6.8 for 2 h, and pH 7.4 for 6 h). The X-ray diffraction (XRD) patterns illustrated that curcumin was in the amorphous or molecular state within PMs. The In vitro release test indicated that Cur-TPGS-PMs possessed a significant sustained-release property. The cell viability in MCF-7 cells was found to be relatively lower in Cur-TPGS-PM-treated cells as compared to free Cur-treated cells. CLSM imaging revealed that mixed micelles were efficiently absorbed into the cytoplasm region of MCF-7 cells. Therefore, Cur-TPGS-PMs could have the significant value for the chronic breast cancer therapy.

Preparation of Ultrafine Polyethylene-Silica Composite Particle with Core-Shell Structure

2012 ◽  
Vol 557-559 ◽  
pp. 554-557 ◽  
Author(s):  
Lei Zu ◽  
Shun Yu Han ◽  
Kai Gu ◽  
Xiu Guo Cui
Keyword(s):  
Shell Structure ◽  
Composite Particle ◽  
Sol Gel ◽  
Core Shell ◽  
Silica Composite ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean ◽  
Core Shell Structure ◽  
Electronic Microscope

A novel ultrafine polyethylene/silica composite particle with core-shell structure was prepared by the sol-gel method in the presence of the melt polyethylene emulsion. A series of samples with different polyethylene content were prepared to investigate the unique characteristics of this original composite particle. The core-shell structure and composition of the composite particle was proved by the transmission electron microscopy observation and Fourier transform infrared spectra. The composite particles possess a spherical morphology and the mean size is about 160nm, presented by the scanning electronic microscope observation and nanoparticle tracking analysis, respectively.

Synthesis of Ce0.8Gd0.05Y0.15O1.9 Nanopowders

2007 ◽  
Vol 336-338 ◽  
pp. 2005-2007
Author(s):  
Xiang Feng Guan ◽  
Zhi Hui Liu ◽  
He Ping Zhou
Keyword(s):  
Electron Microscopy ◽  
Solid Oxide Fuel Cells ◽  
Single Phase ◽  
Fluorite Structure ◽  
Solid Oxide ◽  
Xrd Pattern ◽  
X Ray ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean

Nanosized Ce0.8Gd0.05Y0.15O1.9 powders were synthesized using glycine nitrate process for use as the electrolyte of intermediate temperature solid oxide fuel cells. The powders were characterized by simultaneous thermogravimetry analysis (TGA) and differential thermal analysis (DTA), X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The XRD pattern indicated that the powders exhibited a single phase with cubic fluorite structure. The TEM studies showed that the calcined powders exhibited an almost spherical morphology and the mean size of the particles was 30nm, which is in agreement with the calculated result of XRD.

THE SIZE CONTROL OF UNIFORM NANOCRYSTALLINE Si GRAINS BY CONSTRAINED GROWTH MODEL

2005 ◽  
Vol 19 (15n17) ◽  
pp. 2751-2756 ◽  
Author(s):  
KUNJI CHEN ◽  
KAI CHEN ◽  
PEIGAO HAN ◽  
HECHENG ZOU ◽  
ZHONGYUAN MA ◽  
...  
Keyword(s):  
Size Control ◽  
Nanocrystalline Silicon ◽  
Annealing Conditions ◽  
Crystallization Method ◽  
Transmission Electron ◽  
Mean Size ◽  
Sublayer Thickness ◽  
The Mean ◽  
Size Controlled ◽  
Nanocrystalline Si

Size-controlled nanocrystalline silicon ( nc - Si ) has been prepared from a - SiN x / a - Si:H/a - SiN x ( 'a' standing for amorphous) sandwich structures by thermal annealing. Transmission electron microscope analyses show that the mean size and the grain size distribution (GSD) of the nc - Si are controlled by the annealing conditions and the a - Si sublayer thickness. Based on our theoretical model of constrained crystallization, we interpret the phenomena of the growth halt of nc - Si and higher crystallization temperature for the thinner a - Si sublayers. The experimental results show that constrained crystallization method is promising to achieve uniform and high density nc - Si array which can be used in the future nano-devices.

scholarly journals FABRICATION, CHARACTERIZATION, AND IN VITRO EVALUATION OF PEGYLATED GLYCERIDE LABRASOL® NANOSTRUCTURED LIPID CARRIER COMPOSITES OF METHOTREXATE: THE PATHWAY TO EFFECTIVE CANCER THERAPY

2019 ◽  
pp. 229-237 ◽  
Author(s):  
RADHARANI PANDA ◽  
KETOUSETUO KUOTSU
Keyword(s):  
Sustained Release ◽  
Drug Loading ◽  
In Vitro Release ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carrier ◽  
Transmission Electron Microscopy Analysis ◽  
Transmission Electron ◽  
Electron Microscopy Analysis ◽  
High Entrapment Efficiency

Objective: The objective of the current study is to optimize and evaluate the potential of polyethylene glycolylated (PEG) glyceride Labrasol® nanostructured lipid carrier (NLC) composites of methotrexate (MTX) to achieve enhanced sustained release delivery in cancer treatment. Materials and Methods: MTX-NLC was successfully prepared by hot melt emulsification and probe sonication method for spatial and controlled release of this therapeutic agent. Results: The solubility screening of MTX and lipids resulted in the selection of Monostearin as solid lipid, PEGylated glyceride Labrasol® and olive oil as liquid lipids for the formulation of MTX-loaded NLC composites. Particle size, zeta potential, and polydispersity index of both the composites were confirmed using dynamic light scattering, whereby Labrasol® MTX-NLC showed high entrapment efficiency and drug loading. A spherical particle shape with smooth surface of all the composites was confirmed from the scanning electron microscope and transmission electron microscopy analysis. Labrasol® MTX-NLC showed remarkably increased cytotoxic response, augmented cellular uptake, and low half maximal inhibitory concentration value in MCF-7 cells. In vitro release study confirmed that encapsulation of MTX in PEGylated glyceride Labrasol® MTX-NLC resulted in enhanced sustained release of MTX for a period of 48 h. Conclusion: The present study establishes that PEGylated glyceride Labrasol® MTX-NLC can be considered as a promising anticancer delivery system, thereby improving antitumor efficacy of the drug.

scholarly journals Development of Bioadhesive Microspheres for Oral Bioavailability Enhancement of Berberine Hydrochloride

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Ye Zhang ◽  
Hongming Liu
Keyword(s):  
Sustained Release ◽  
Drug Loading ◽  
Gastric Ulcers ◽  
Pharmacokinetic Studies ◽  
Sustained Delivery ◽  
Gelatin Microspheres ◽  
Bioavailability Enhancement ◽  
The Mean

The objective of this study was to innovatively prepare chitosan-coated alginate/gelatin BBH loaded microspheres and evaluate their pharmaceutical characteristics and pharmacokinetics. The bioadhesive microspheres were prepared using an emulsification technique. Three batches of microspheres were formed and their stability was evaluated. BBH loaded microspheres were almost spherical with shallow elevation on surfaces. The mean particle size of microspheres was 368.2 μm, drug loading was3.59±0.01%, andin situbioadhesion percentage was91.23%±8.2%and they achieved a sustained release with 71.29% for 8 hoursin vitro. Pharmacokinetic studies in rats indicated that the bioavailability of BBH microspheres was enhanced about 1.5-fold as compared with commercial tablets. BBH microspheres exhibited a sustained-release profile over 48 h. Thus, chitosan-coated alginate/gelatin BBH loaded microspheres which combined the advantages of alginate/gelatin microspheres and chitosan may be used as a sustained delivery system for BBH to treat duodenal and benign gastric ulcers.

scholarly journals Модификация структурно-морфологических свойств германия в многослойной нанопериодической структуре Al-=SUB=-2-=/SUB=-O-=SUB=-3-=/SUB=-/Ge с промежуточными слоями Si при отжиге

2021 ◽  
Vol 55 (10) ◽  
pp. 882
Author(s):  
М.В. Байдакова ◽  
Н.А. Берт ◽  
В.Ю. Давыдов ◽  
А.В. Ершов ◽  
А.А. Левин ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Heat Treatment ◽  
Annealing Temperature ◽  
Electron Beam Evaporation ◽  
X Ray Diffraction ◽  
Bimodal Size Distribution ◽  
X Ray ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean

Multi-layered nanosized Al2O3/Ge/Si structures manufactured by electron-beam evaporation and annealed at a temperature within the range 700−900◦C are examined using transmission electron microscopy, Raman spectroscopy and X-ray diffraction techniques. The periodic structure with a good layer planarity is confirmed to retain after heat treatment up to 900◦C. At an annealing temperature above 700◦C, nanocrystallites with a bimodal size distribution start to form within initially amorphous Ge layers, the mean size of small crystallites being determined by Ge layer thickness and annealing temperature. An essential loss of Ge from multi-layered structure after 900oC anneal and development of Ge1−x Six solid solution with x up to 0.07 in the nanocrystallites is revealed.

Konjac glucomannan nanocrystals prepared by acid hydrolysis

e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Jin Huang ◽  
Shanjun Gao ◽  
Xiaomeng Shen
Keyword(s):  
Acid Hydrolysis ◽  
Amorphous State ◽  
Konjac Glucomannan ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean ◽  
Hydrolysis Of ◽  
Oval Shape

AbstractKonjac Glucomannan (KGM) nanocrystals were prepared from the disruption of KGM by acid hydrolysis. Effect of hydrolysis time on the degree of hydrolysis of KGM and the size of nanocrystals were investigated. Results from Infrared spectroscopy (IR) showed that KGM nanocrystals were still composed of mannose and glucose like the raw KGM. Results from X-ray diffractometer (XRD) revealed that the amorphous state of raw KGM had changed into a typical β-type crystalline state of KGM nanocrystals. Results from transmission electron microscopy (TEM) indicated that irregularly oval shape nanocrystals with the mean size of 15-40 nm were observed.

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