Isolation and Characterization of a Strain of Acidithiobacillus ferrooxidans

An acidophilic strain was isolated from drainage in Shanxi coal mine, after 16SrDNA amplification and sequencing analysis, it was identified to be Acidithiobacillus ferrooxidans. The ferrous ion oxidation rate of At.f and pH value were studied during its growth, the results showed that, the strain entering into the exponential growth phase needed about 30 hours, and the oxidation rate was more than 99% after 60 hours. The pH value increased from 2.08 to 2.64 at first 30 hours and then decreased to 2.0, and finally maintained at 2.0 in the whole growth process. The isolated strain provided a potential alternative method to handle the steel-picking sulfuric acid waste liquor.

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Manipulating the Structure and Morphology of Cobalt-Boron Nanoparticles through a Chemical Approach

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.829.762 ◽

2013 ◽

Vol 829 ◽

pp. 762-766 ◽

Cited By ~ 1

Author(s):

Hooman Sabarou ◽

Abolghasem Ataie

Keyword(s):

Growth Process ◽

Ph Value ◽

Amorphous Structure ◽

Detailed Characterization ◽

Synthesis Mechanism ◽

Subsequent Formation ◽

Structure And Morphology ◽

Morphology And Structure ◽

Structure Phase

Cobalt-boron nanoparticles have been synthesized by a chemical reaction between NaBH4 and CoCl2.6H2O through manipulating pH value of the reaction mixture. The morphology, structure, phase composition, and thermal behavior have been examined via FESEM, TEM, XRD, EDS, and DSC techniques, respectively. It is demonstrated that the morphology and structure of ultimate nanoparticles completely depends on the pH value of reaction mixture. While the neutral pH value favors the smallest nanoparticles with a mean particle size of 50 nm and complete amorphous structure, the acidic condition promotes the growth process and the crystal structure. Furthermore, these nanoparticles transform into cobalt nanocrystallites after heated at 600°C, and retained the discrepancies in the morphology and the structure of the parent cobalt-boron nanoparticles. A detailed characterization of the nanoparticulates, discussions on the synthesis mechanism, and subsequent formation transformation have been provided.

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Preparation and Characterization of Cu-Coated Nano SiC Composite Particles

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.373-374.674 ◽

2008 ◽

Vol 373-374 ◽

pp. 674-677 ◽

Cited By ~ 2

Author(s):

Sheng Zhu ◽

Chao Li ◽

Jian Hua Du ◽

Wen Zheng Han

Keyword(s):

Reaction Mechanism ◽

Heterogeneous Nucleation ◽

Oxidation Rate ◽

Ph Value ◽

Composite Particles ◽

Nucleation Process ◽

Sic Particles ◽

Reaction Solution ◽

Reaction Speed

The Cu-coated nano SiC composite particles were prepared by heterogeneous nucleation method. For the heterogeneous nucleation process, the dispered nano SiC particles and the active Cu2O precipitation were both prefabricated. The composite particles were characterized by means of XRD, SEM, EDS, TEM and ED analysis technics. It is found that Cu layers are coated on the nano SiC particles, which are continuous and dense, and partly oxidized to Cu2O, and the oxidation rate can be reduced by improving experimental processes. The investigation on reaction mechanism shows the disproportionating reaction speed of Cu+ can be controlled by adjusting the pH value of reaction solution, which makes the degree of supersaturation of Cu crystallites accord with the conditions of heterogeneous nucleation.

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Isolation and Characterization of a Single-Stranded RNA Virus Infecting the Bloom-Forming Diatom Chaetoceros socialis

Applied and Environmental Microbiology ◽

10.1128/aem.02580-08 ◽

2009 ◽

Vol 75 (8) ◽

pp. 2375-2381 ◽

Cited By ~ 53

Author(s):

Yuji Tomaru ◽

Yoshitake Takao ◽

Hidekazu Suzuki ◽

Tamotsu Nagumo ◽

Keizo Nagasaki

Keyword(s):

Rna Virus ◽

Burst Size ◽

Host Cells ◽

Sequencing Analysis ◽

Virus Family ◽

Isolation And Characterization ◽

Ssrna Virus ◽

Rhizosolenia Setigera ◽

Virus Clone

ABSTRACT Diatoms are very significant primary producers in the world's oceans. Various environmental factors affect the depletion of diatom populations. The importance of viruses as a potential mortality source has recently been recognized. We isolated and characterized a new diatom virus (Chaetoceros socialis f. radians RNA virus [CsfrRNAV]) causing the lysis of the bloom-forming species Chaetoceros socialis Lauder f. radians (Schütt) Proschkina-Lavrenko. The virus infectious to C. socialis f. radians was isolated from water samples collected in Hiroshima Bay. Here we show the physiology, morphology, and genome characteristics of the virus clone. Virions were 22 nm in diameter and accumulated in the cytoplasm of the host cells. The latent period and the burst size were estimated to be <48 h and 66 infectious units per host cell, respectively. CsfrRNAV harbors a single-stranded RNA (ssRNA) genome and encodes at least three polypeptides of 32.0, 28.5, and 25.0 kDa. Sequencing analysis shows the length of the genome is 9,467 bases, excluding a poly(A) tail. The monophyly of CsfrRNAV and other diatom-infecting RNA viruses, Rhizosolenia setigera RNA virus and Chaetoceros tenuissimus RNA virus, was strongly supported by phylogenetic analysis based on the amino acid sequence of the RNA-dependent RNA polymerase domains. This suggested a new ssRNA virus family, Bacillariornaviridae. This discovery of CsfrRNAV may aid in further understanding the ecological dynamics of the C. socialis f. radians population in nature and the relationships between ssRNA diatom viruses and their hosts.

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Isolation and characterization of sodium alginate from brown algae Sargassum sp. for making tenggiri (Scomberomorus commerson) meatballs

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f070201 ◽

2011 ◽

Vol 7 (2) ◽

pp. 59-67

Author(s):

WIWIN DWI WARDANI ◽

KAWIJI KAWIJI ◽

GODRAS JATI MANUHARA

Keyword(s):

Sodium Alginate ◽

Brown Algae ◽

Ph Value ◽

Chemical Characteristic ◽

Isolation And Characterization ◽

Randomized Design ◽

Scomberomorus Commerson ◽

Water Rate ◽

Completely Randomized Design

Wardani WD, Kawiji, Manuhara GJ. 2009. Isolation and characterization of sodium alginate from brown algae Sargassum sp. for making tenggiri (Scomberomorus commerson) meatballs. Biofarmasi 7: 59-67. Brown algae Sargassum sp. widespread in territorial of Indonesia. Sargassum sp. can be extracted for the yield compound of sodium alginate that can be applied in making the tenggiri meatballs to take care the emulsion stability and to repair the properties of rheology. This research used a Completely Randomized Design (CRD) with one factor that was a variation of sodium alginate addition, i.e. F1 (0% alginate), F2 (0.25% alginate), F3 (0.5% alginate), F4 (0.75% alginate) and F5 (0.5% STPP). The result of research showed the characteristics of sodium alginate of seaweeds Sargassum sp. from the extraction result included: water rate [5.94% (wb)]; ash rate [19.62% (wb) and 20.86% (db)]; the pH value of condensation of sodium alginate 0.1% (9.07), the pH value of condensation of sodium alginate 0.2% (9.07), the pH value of condensation of sodium alginate 0.3% (9.06), water absorption (214.44%), and rendement (31.62%). The addition of sodium alginate in the making tenggiri meatballs improved the hardness and elasticity of meatballs. The highest hardness level of tenggiri meatballs was formula F4 (0.75% alginate) and the highest elasticity level of tenggiri meatballs was with an addition of sodium alginate formula F4. The result of organoleptic test indicated that an addition of sodium alginate tends to improve panelist pleasure to color, flavor, taste, elasticity and hardness of tenggiri meatballs. The tenggiri meatballs formula F4 represented the formula which the most liked by the panelists. The chemical characteristic of tenggiri meatballs formula F4 including: water rate (74.61%), ash rate (1.66%), protein rate (14.53%), fat rate (0.93%) and carbohydrate rate (8.26%).

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Isolation and characterization of Acidithiobacillus ferrooxidans strain QXS-1 capable of unusual ferrous iron and sulfur utilization

Hydrometallurgy ◽

10.1016/j.hydromet.2013.03.005 ◽

2013 ◽

Vol 136 ◽

pp. 51-57 ◽

Cited By ~ 13

Author(s):

Yanfei Zhang ◽

Yu Yang ◽

Jianshe Liu ◽

Guanzhou Qiu

Keyword(s):

Acidithiobacillus Ferrooxidans ◽

Ferrous Iron ◽

Isolation And Characterization

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Isolation and Characterization of a Rhizobium Bacterium Associated with the Toxic Dinoflagellate Gambierdiscus balechii

10.1101/789107 ◽

2019 ◽

Author(s):

Zhen Wu ◽

Xiaohong Yang ◽

Senjie Lin ◽

Wai Hin Lee ◽

Paul K.S. Lam

Keyword(s):

16S Rdna ◽

Net Primary Productivity ◽

Future Research ◽

Exponential Growth Phase ◽

Sequencing Analysis ◽

Nutrient Regeneration ◽

Toxic Dinoflagellate ◽

First Report ◽

Isolation And Characterization ◽

Encoding Gene

ABSTRACTAlgae-bacteria associations are increasingly being recognized to be important in shaping the growth of both algae and bacteria. Bacteria belonging to order Rhizobiales are important symbionts of legumes often developing as nodules on plant roots, but have not been widely documented in association with algae. Here, we detected, isolated, and characterized a Rhizobium species from the toxic benthic dinoflagellate Gambierdiscus culture. The sequence of 16S rDNA showed 99% identity with that of Rhizobium rosettiformans. To further characterize the bacterium, we amplified and sequenced a cell wall hydrolase (CWH)-encoding gene; phylogenetic analysis indicated that this sequence was similar to the homologs of Martellela sp. and Hoeflea sp, of order Rhizobiales. We performed PCR using nifH primers to determine whether this bacterium can fix N2; however, the results of sequencing analysis showed that it was closer to chlorophyllide a reductase-encoding gene (bchX), which is similar to nifH. Results of 16S rDNA qPCR showed that compared to that in the early exponential phase, the abundance of this bacterium increased during the late exponential growth phase of Gambierdiscus. When the dinoflagellate culture was subjected to N limitation, the abundance of the bacterium represented by both 16S rDNA and CWH increased. Based on these results and published literature, it is apparent that this Rhizobium bacterium benefits from the association with Gambierdiscus by hydrolyzing and utilizing the extracellular organic matter exudates released by the dinoflagellate. This is the first report of Rhizobium species being associated with dinoflagellates, which will shed light on the algae-bacteria relationships.IMPORTANCEPhytoplankton are the undisputed primary producers in the aquatic ecosystems and contribute approximately half of the global net primary productivity.Dinoflagellates are one of the most important phytoplankton in the marine ecosystems. Commonly, they do not exist autonomously in the marine environment but rather co-live with many bacteria that interact with dinoflagellates, producing a dynamic microbial ecosystem. Their interactions play a major role in important processes such as carbon fluxes and nutrient regeneration in the ocean, ultimately influencing the global carbon cycle and the climate. Hence, there is a need to understand the association and relationships between dinoflagellates and bacteria. Here, we tried to elucidate these interactions through isolating and characterizing a bacterium from a benthic toxic dinoflagellate culture. Our study is the first report of such bacterium being recorded to be associated with a dinoflagellate in this genus, providing new insights into the dinoflagellate-bacteria association for future research.

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ISOLASI DAN KARAKTERISASI KOLAGEN DARI KULIT IKAN PATIN (Pangasius sp.)

Jurnal Sains Natural ◽

10.31938/jsn.v8i1.106 ◽

2018 ◽

Vol 8 (1) ◽

pp. 8 ◽

Cited By ~ 2

Author(s):

Pipih Suptijah ◽

Dini Indriani ◽

Supriyono Eko Wardoyo

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Acid Composition ◽

Ph Value ◽

Helical Structure ◽

Color Analysis ◽

Alternative Source ◽

Isolation And Characterization ◽

Naoh Solution

Isolation and Characterization of Collagen from the Skin of Catfish (Pangasius sp.) Skin of catfish is one of aquatic by-products which could be used as an alternative source of collagen. This research is aimed to isolate and characterize collagen from skin of catfish. Methods of isolation of collagen included three stages, the first was deproteinization using NaOH solution with concentration of 0.05 M; 0.10 M; 0.15 M; 0.20 M for 12 hours, the second was soaking in CH3COOH solution with concentration of 0.05 M; 0.10 M; 0.15 M; and 0.20 M for 2 hours, and the third was extraction in water at a temperature of 40 0C for 2 hours; characterization of collagen was included chemical and physical properties. The results showed that the best extraction method ofcollagen from skin of catfish was soaking the skin in 0.05 M NaOH solution for 12 hours and soaking the skin in 0.05 M acetic acid for 2 hours. Extraction yields of collagen was 12.15%. Chemical characteristics included proximate and amino acid composition. Proximate value of collagen consisted of moisture was 6.55%, ash 1.80%, protein 64.74% and fat 8.85%. The major amino acid composition of collagen were glycine, proline, alanine, arginine and glutamate. Physical characteristics of collagen resulted from FTIR analysis showed amide A, amide B, amide I, amide II and amide III, triple helical structure of the amide I and amide III indicates that the compound produced was collagen; color analysis was 66.39%; thermal analysis showed a melting temperature peak was 154.47 0C and pH value was 5.34.Keywords : Catfish, isolation, characterization, collagen, skin ABSTRAK Kulit ikan patin merupakan salah satu limbah hasil perairan yang dapat digunakan sebagai sumber alternatif kolagen. Penelitian ini bertujuan untuk mengisolasi dan karakterisasi kolagen yang diperoleh dari kulit ikan patin. Isolasi kolagen yang dilakukan meliputi tiga tahap, yaitu tahap pertama adalah proses deproteinisasi menggunakan larutan NaOH dengan konsentrasi, yaitu 0,05 M; 0,10 M; 0,15 M; 0,20 M dan lama waktu perendaman selama 12 jam; tahap kedua, yaitu perendaman dalam larutan CH3COOH dengan empat konsentrasi CH3COOH yaitu 0,05 M; 0,10 M; 0,15 M; dan 0,20 M dan lama waktu perendaman selama 2 jam; dan tahap ketiga, yaitu ekstraksi dengan air pada suhu 40 0C selama 2 jam; serta karakterisasi kolagen yang dilakukan, meliputi sifat kimia dan fisik. Hasil penelitian menunjukkan bahwa metode ekstraksi kolagen dari kulit ikan patin terbaik diperoleh melalui proses perendaman kulit dalam larutan NaOH 0,05 M selama 12 jam dan perendaman kulit dalam asam asetat 0,05 M selama 2 jam. Rendemen serbuk kolagen yang dihasilkan sebesar 12,15 %. Karakteristik kimia meliputi proksimat dan komposisi asam amino. Nilai proksimat kolagen terdiri dari kadar air 6,55 %, abu 1,80 %, protein 64,74 % dan lemak 8,85 %. Komposisi asam amino yang dominan pada kolagen adalah glisina, prolina, alanina, arginina dan glutamat. Karakteristik fisik kolagen yang dihasilkan adalah analisis FTIR menunjukkan adanya gugus amida A, amida B, amida I, amida II dan amida III, struktur triple heliks pada amida I dan amida III mengindikasikan bahwa senyawa yang dihasilkan adalah kolagen; analisis warna yaitu 66,39 %; analisis termal yang menunjukkan suhu puncak pelelehan adalah 154,47 0C dan nilai pH kolagen yaitu 5,34. Kata kunci : Ikan patin, isolasi, karakterisasi, kolagen, kulit

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