Effects of Water Circulation and Quantity on GABA Content and some Properties of Germinated Brown Rice

2014 ◽  
Vol 931-932 ◽  
pp. 1529-1533
Author(s):  
Sirorat Pilawut ◽  
Winit Chinsuwan

At germination stage, paddy rice seed produces phytochemicals that are essential for human health. One way of value added concept for rice production is to germinate paddy rice grain to increase nutritional quality before selling. Typical method of soaking paddy grain in stagnant water for 1 to 2 days has been employed for germination. Water flowing through rice grains instead of stagnant may increase its nutritional quality. The objective of this study was to compare 2 methods of grain watering, i.e. soaking in stagnant water and circulating water through grains, on gamma aminobutyric acid (GABA) content and some other properties. The appropriate proportion of water used was also to be evaluated. The Khoa Dok Mali 105 rice variety was chosen for germination tests. The soaking and circulation methods were compared at 8 levels of water quantity i.e. 3, 4, 5, 6, 7, 8, 9 and 10 times of paddy rice by weight. The results showed GABA content of paddy rice under circulation method was higher for all water quantities. The higher the amount of water, the higher the GABA content in both watering methods.

2020 ◽  
Vol 49 (3) ◽  
pp. 356-363
Author(s):  
E.E. Sage ◽  
Y.X. Chang ◽  
R. Wickneswari ◽  
M.M. Mackeen

The neurotransmitter γ-aminobutyric acid (GABA) is a functional food ingredient of growing importance in the preparation of GABA-enriched germinated brown rice (GBR). Quantification of GABA levels during germination in a locally developed high yielding red rice variety (UKMRC-9) was carried out via high-performance liquid chromatography (HPLC) analysis using pre-column chemical derivatisation with 2-hydroxynaphthaldehyde (HN) using a UV detection system. Factors that influence rice germination such as soaking time and temperature were also studied. The results of this study showed that the UKMRC-9 rice variety soaked in water for 6 hours at 35 °C yielded the highest value for both germination percentage (95.4±1.2%) and GABA content (411 μg g-1). This suggests that long soaking times of 1-4 days can be substituted by short soaking times to produce high GABA levels in germinated brown rice.


2018 ◽  
Vol 15 (2) ◽  
Author(s):  
Nicole Colón Carrión ◽  
Chad Lozada Troche

Crops and stored grains are susceptible to pathogens that represent a threat to our health. The study presented herein compares the normal surface and endophytic fungal communities present on white and brown rice grains. One hundred grains of each rice variety was analyzed to determine their fungal contaminants and endophytes. Fungi were inoculated on SDA media, and purified in PDA media; morphological characterization was performed followed by amplification of the ITS region using PCR for all fungal isolates. Statistical analysis indicated significant differences between medium brown rice compared to white rice for surface and endophytic communities (p-value £ 0.05). In addition, a higher fungal diversity was found on brown rice grains compared to white rice. This variation may be due to differences in the processing methods used for each rice grain type. BLAST analysis revealed the presence of toxigenic strains of Aspergillus flavus, A.oryzae, Penicillium verrucosum, and P. viridicatum. The study of fungal growth in rice grains can contribute to the minimization of mycotoxin production by its prevention and control; therefore, decreasing crop contamination and human exposure to their metabolites. KEYWORDS: Fungi; Rice; Fungal contaminants; Fungal endophytes


Weed Science ◽  
1983 ◽  
Vol 31 (1) ◽  
pp. 120-123 ◽  
Author(s):  
Roy J. Smith

Yields of drill-seeded paddy rice (Oryza sativaL. ‘Lebonnet’) at optimum stands of 215 to 270 plants/m2at Stuttgart, Arkansas, were reduced 9, 18, 20, and 36% by bearded sprangletop [Leptochloa fascicularis(Lam.) Gray] densities of 11, 22, 54, and 108 plants/m2, respectively. There was a linear decrease in rice grain yield of 21 kg/ha for each bearded sprangletop plant per square meter. Weed densities of 54 and 108 plants/m2reduced head-rice yields (whole milled kernels) and a density of 108 plants/m2reduced germination of rice seed. The number of bearded sprangletop panicles produced per weed plant decreased as the weed density increased.


2014 ◽  
Vol 70 (6) ◽  
Author(s):  
Zuwariah Ishak ◽  
Hasri Hassan ◽  
Siti Adira Mohd Zaidun

The aim of this study was to evaluate the gamma amino butyric acid (GABA) and amino acid content in products obtained from MR 219 germinated brown rice. The investigation included MR 219 white rice, MR 219 brown rice, MR 219 germinated brown rice (GBR) with different germination time and heat treatment and commercial germinated brown rice. In this study GABA content in germinated brown rice variety MR 219 (35.59 mg/100 g) was significantly higher (p<0.05) than commercial germinated brown rice (12.60 mg/100 g), MR 219 brown rice (1.43 mg/100 g) and MR 219 white rice (0.82 mg/100 g). Results indicated that heat treatment significantly alter the amount of GABA, serine, glutamic, histidine, threonine, alanine, proline, valine, lysine, isoleusine and leucine. It was found that the essential amino acids; histidine, threonine, tyrosine, valine, lysine, isoleucine, leucine and phenylalanine showed significant increase (P<0.05) in MR 219 germinated brown rice compared to other germinated brown rice samples.


Author(s):  
Nirandorn Chanlat ◽  
Sirichai Songsermpong ◽  
Chulaluck Charunuch ◽  
Onanong Naivikul

Pre-germinated brown rice (PGBR) from non-glutinous (KDML 105) and glutinous (RD 10) varieties was produced by a soaking method in cool water at 25°C for 48 h. After germination, increased crude fiber, reducing sugar (P ? 0.05) and the ?-aminobutyric acid (GABA) content (P ? 0.01) and decreased fat were observed. The extrusion was carried out in a twin-screw extruder by a central composite design (CCD) which studied the effect of feed moisture (15.6-22.3%), screw speed (264-434 rpm) and pre-germinated glutinous brown rice flour level (0-63.6%) on the physicochemical properties of extruded snacks (expansion ratio, density, hardness, brittleness, water absorption index (WAI), water solubility index (WSI)) and GABA content. Second-order polynomial models were computed and used to generate surface plots. Increasing the feed moisture increased the density (P ? 0.01) hardness and WAI, and decreased the expansion ratio, brittleness (P ? 0.01) and WSI (P ? 0.05). Increasing the screw speed increased the expansion ratio, brittleness, WAI and WSI, and decreased the density and hardness. Increasing the levels of pre-germinated glutinous brown rice flour increased the expansion ratio (P ? 0.01), brittleness, WSI (P ? 0.01) and the GABA content (P ? 0.01), and decreased the density, hardness (P ? 0.01) and WAI (P ? 0.01), while the feed moisture content and screw speed had no significant effect on the GABA content (P > 0.05). This knowledge can be used in the design of customer-oriented extruded PGBR products.


2019 ◽  
Vol 57 (3B) ◽  
pp. 116
Author(s):  
Van Anh Luu ◽  
Hang Thanh Nguyen ◽  
Giang Truong Nguyen

ABSTRACT – QMFS 2019Germinated brown rice strains contain more bioactive substances than germinated regular rice ones, however germination conditions play an important role in the activity and the content of those substances. The proper germination process provides the optimized active ingredients from rice that can be used for the production of nutritious beverages. In this study, we investigated the effects of pH, temperature and incubation time in microaerobic culture condition on the change of bioactive substances in AnhDao brown rice. The optimal germination condition with pH at 3, temperature of 35 0C and time for 36h release 109.11U/g of the α-amylase activity, 17.22(U/g) of the enzyme glutamate decacboxylase (GAD), 1.38(U/g) of protease, 231.76mg/100g of GABA content and 21.9 (mgGAE/100g) of polyphenol from germinated AnhDao brown rice. In nutrient evaluation, germinated AnhDao brown rice contains 65.53% of starch, 2.49% of lipid, 9.13%of protein, 2.04% of reducing sugar, and 1.26% of ash.Key words: Germinated brown rice, bioactive substances, α-amylase, protease, glutamate decacboxylase (GAD), Gamma aminobutyric acid (GABA).


2018 ◽  
Vol 9 (3) ◽  
pp. 123-127
Author(s):  
Anh Van Luu ◽  
Thi Yen Nguyen ◽  
Thi Thuy An Ho ◽  
Thi Thanh Hang Nguyen ◽  
Truong Giang Nguyen

Brown rice is a food ingredient which has high nutritious values. During germination, some nutritious and functional components are increased such as lysine, vitamin E, B1, B6, magnesium, calcium, iron… and especially γ – amino butyric acid. Enzyme activity will also change during the germination of the grains. The α – amylase activity of ungerminated grains is very low, only 34.91 UI/g. This is because the enzyme is hibernating and not activated. During germination, enzyme activity will increase. Submerge the Anhdao brown glutinous rice for 6 hours at 30°C in solutions with different pH values (2.0, 3.0, 4.0, 5.0, 6.0). The results show that at pH 3.0 the activity of α – amylase enzyme reaches the highest value of 82.93 UI/g. After the submersion, incubate the germinated brown rice in unentirely anaerobic condition at different temperature of 25, 30, 35, 37°C. The result showed that at 35°C after 24 hours of incubation, the α – amylase activity reaches the highest value of 89.82 UI/g. Examine the dynamic of changes of α – amylase activity against time at 35°C, we can see that in the first 28 hours the α – amylase activity increased significantly. Highest α – amylase activity reaches 97.10 UI/g after 28 hours of incubation. In reality, people usually use enzyme from germinated grains for many food manufacturing industries. α – amylase activity increases during incubation, which can bring promising prospects for processing sugar syrup and prebiotics food from germinated rice. Gạo lứt là một nguyên liệu thực phẩm có giá trị dinh dưỡng cao. Trong quá trình nảy mầm các thành phần dinh dưỡng và chức năng của hạt gạo lứt được tăng lên ví dụ như lysine, các vitamin E, B1, B6, magie, canxi, sắt… và đặc biệt là γ – amino butyric acid. Hoạt tính của hệ enzyme sẽ thay đổi trong suốt quá trình nảy mầm của hạt gạo. Hoạt tính enzyme α – amylase của hạt gạo chưa nảy mầm là rất thấp, chỉ đạt 34,91UI/g, do enzyme của hạt đang ở trạng thái ngủ chưa được kích hoạt. Trong quá trình nảy mầm thì hoạt tính của α – amylase tăng lên. Tiến hành ngâm gạo lứt giống nếp Anh Đào trong 6 tiếng, ở 30°C trong nước ngâm có pH khác nhau (pH 2.0, 3.0, 4.0, 5.0, 6.0). Kết quả cho thấy, ở pH 3.0 hoạt độ enzyme α – amylase cao nhất đạt 82,93 UI/g. Sau quá trình ngâm, tiến hành ủ nẩy mầm gạo lứt yếm khí không hoàn toàn ở những nhiệt độ khác nhau 25, 30, 35, 37°C, kết quả ở 35°C sau 24 giờ ủ hoạt độ enzyme α – amylase đạt cao nhất là 89,82 UI/g. Khảo sát động học sự thay đổi của hoạt độ enzyme α – amylase theo thời gian ở nhiệt độ ủ 35°C, kết quả cho thấy,trong 28 giờ đầu hoạt độ của α – amylase tăng mạnh. Hoạt độ α – amylase cao nhất đạt 97,10 UI/g sau 28 giờ ủ. Trong thực tế người ta đã sử dụng enzyme từ hạt nảy mầm cho rất nhiều ngành sản xuất thực phẩm. Hoạt độ của α-amylase sau quá trình ủ mầm tăng lên, có thể đem lại triển vọng sử dụng để chế biến dịch đường và các sản phẩm có tính prebiotic từ gạo lứt nảy mầm.


2010 ◽  
Vol 16 (2) ◽  
pp. 147-158 ◽  
Author(s):  
W. Watchararparpaiboon ◽  
N. Laohakunjit ◽  
O. Kerdchoechuen

Germinated brown rice (GBR) of two popular Thailand varieties, Khao Dawk Mali 105 (KDML 105) and Chainat 1, with improved nutritional composition, was obtained by optimizing water soaking conditions. Different water pH (3, 4, 6 and 8), temperatures (25 °C, 35 °C and 45 °C), and soaking times (12 and 24 h) were tested. Using the response surface methodology (RSM), the best condition for producing GBR of both varieties was soaking in water with pH 6 and temperature of 35 °C for 24 h. It caused a 4- to 5-fold increase in gamma-amino butyric acid (GABA) content which, together with protein and lipid contents, were highest among treatments. Intermediate levels of thiamine (vitamin B1) and phytic acid (IP6) were obtained. In GBR of KDML 105 variety, GABA, vitamin B1 and IP6 contents were 16.48, 0.526 and 501.06 mg/100 g, respectively, while protein and lipid contents were 10.50% and 4.00%, respectively. For Chainat 1 variety, GABA, vitamin B1 and IP6 contents were 14.50, 0.436 and 486.03 mg/100 g, respectively, while protein and lipid contents were 9.80% and 3.99%, respectively. Carbohydrate and amylose contents differed by only less than 1-2% among treatments. Supplemental aeration during water soaking decreased GABA, protein and lipid contents, but increased vitamin B1 and IP6 contents in both varieties. Furthermore, cooked GBR of both varieties had softer texture than cooked ordinary brown rice.


Sign in / Sign up

Export Citation Format

Share Document