Porous Biphasic Calcium Phosphate for Biomedical Application

Excellent osteoconductivity and resorbability achieved when porous bioceramics have highsurface area that providing fast bone ingrowth. Porous samples were fabricated by using biphasic calcium phosphate BCP (achieved from HA heat treated at 850 oC) with 10 and 20 wt% of ovalbumin binder powder and mixture of carrot fibers and ovalbumin powders (1:1) then dried at 60oC and fired at 1300 oC. Structural, physical and mechanical properties of the prepared porous bioceramic were determined involved X-ray diffraction, Fourier transform infrared spectroscopy FTIR, apparent porosity, water absorption, apparent solid density and compressive strength. The results of X-ray and FTIR showed that the heat treatment of HA was succeeded in forming biphasic calcium phosphate. The apparent porosity values increased with increasing of the binder and carrot fibers content and the growths density of bacteria on bioceramics are less than natural bone. The effect of pathogenic bacteria (Pseudomonas & Staphylococcus) that cause pollution on porous calcium phosphate and natural bone (Albino mice) has been studied.

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Five Years Clinical Follow Up Bone Regeneration with CaP Bioceramics

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1339 ◽

2007 ◽

Vol 361-363 ◽

pp. 1339-1342 ◽

Cited By ~ 3

Author(s):

Clemencia Rodríguez ◽

Alain Jean ◽

Sylvia Mitja ◽

G. Daculsi

Keyword(s):

Calcium Phosphate ◽

Bone Regeneration ◽

Biphasic Calcium Phosphate ◽

Tooth Extraction ◽

Alveolar Bone ◽

Bone Ingrowth ◽

X Ray ◽

Dental Implantation ◽

Preventive Method

To overcome autograft use for dental implantation, it is important to prevent bone loss after tooth extraction or to restore alveolar bone level after pathological diseases. Biphasic calcium phosphate (BCP), mixture of HA and ß-TCP, have proven its performance in orthopaedic, while few studies have been reported in dentistry. We reported 5 years clinical follow up on bone regeneration after immediate dental root filling. MBCP 60/40 and MBCP 20/80 are biphasic CaP intimate mixture of HA/TCP 60/40 and 20/80; with interconnected macroporosity and microporosity. Forty cases have been distributed in two groups for alveolar pocket filling. Seven cases without filling are used as control. X-Ray at 0, 3, 6, 12 months and 5 years follow up for some patients were performed. In all the 40 cases, radio-opacity of the implantation area decreases on time, indicating resorption and bone ingrowths at the expense of the two bioceramics. No difference in the resorption kinetics appeared on X-Ray. After 1 year, the implantation area looks as physiological bone and is maintained on time. The newly formed bone is preserved after 5 years contrarily to the controls cases (without filling)where we observed decrease of 2 to 5 mm. This study demonstrated that immediate filling of alveolar pocket after tooth extraction is a preventive method of the jaw bone resorption. After long term (other one year) resorption and bone ingrowth were demonstrated for both micro and macroporous biphasic calcium phosphate with two different HA/TCP ratio.

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Highly efficient biphasic calcium-phosphate coating procedure with an enhanced coating yield and protein incorporation rate

Materials Express ◽

10.1166/mex.2021.2002 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1428-1437

Author(s):

Ping Sun ◽

Shuyi Li ◽

Jianhua Niu ◽

Min Yi ◽

Weixing Xu ◽

...

Keyword(s):

Calcium Phosphate ◽

Biomedical Applications ◽

Biphasic Calcium Phosphate ◽

Biomedical Application ◽

Calcium Phosphate Coating ◽

Phosphate Solution ◽

X Ray Diffraction ◽

X Ray ◽

Coating Procedure ◽

Protein Incorporation

A biphasic calcium-phosphate (CaP)-coating is a promising surface modification for functionalizing various endosseous biomaterials. However, its biomedical application is limited by its low coating yield and incorporation inefficiency. We developed a highly concentrated (4.5×) supersaturated calcium-phosphate solution (SCPS) and compared its physicochemical properties with those of 1× SCPS. One milliliter of 4.5× SCPS formed a thick (110 μm) continuous coating on a titanium disc (4×4×1 mm), compared to the thin (29 μm) 1× SCPScoating. On X-ray diffraction analysis, the 4.5× SCPS-coating had characteristic dicalcium-phosphate dehydrate and apatite peaks, in contrast to the apatite-only of 1× SCPS-coating. Under acidic condition (pH 4.5), the 4. × 5SCPS-coating released significantly less Ca2+ than the 1× SCPS-coating. FITC-bovine serum albumin incorporation in the 4.5× SCPS-coating (81.20±6.42%) was significantly higher than in the 1× SCPS-coating (21.86±1.90%). Thus, this modified coating procedure holds promise for biomedical applications.

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The Fabricated Collagen-Based Nano-Hydroxyapatite/β-Tricalcium Phosphate Scaffolds

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.506.57 ◽

2012 ◽

Vol 506 ◽

pp. 57-60 ◽

Cited By ~ 2

Author(s):

M. Ebrahimi ◽

Naruporn Monmaturapoj ◽

S. Suttapreyasri ◽

P. Pripatnanont

Keyword(s):

Mechanical Properties ◽

Solid State ◽

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Single Phase ◽

Physical And Mechanical Properties ◽

Total Porosity ◽

Xrd Pattern ◽

Collagen Coating ◽

Dimensional Shrinkage

The biphasic calcium phosphate (BCP) concept was introduced to overcome disadvantages of single phase biomaterials. In this study, we prepared BCP from nanoHA and β-TCP that were synthesized via a solid state reaction. Three different ratios of pure BCP and collagen-based BCP scaffolds (%HA/%β-TCP; 30/70, 40/60 and 50/50) were produced using a polymeric sponge method. Physical and mechanical properties of all materials and scaffolds were investigated. XRD pattern proved the purity of each HA, β-TCP and BCP. SEM showed overall distribution of macropores (80-200 µm) with appropriate interconnected porosities. Total porosity of pure BCP (93% ± 2) was found to be higher than collagen-based BCP (85%± 3). It was observed that dimensional shrinkage of larger scaffold (39% ± 4) is lower than smaller one (42% ± 5) and scaffolds with higher HA (50%) ratio experienced greater shrinkage than those with higher β-TCP (70%) ratio (45% ±3 and 36% ±1 respectively). Mechanical properties of both groups tend to be very low and collagen coating had no influence on mechanical behavior. Further studies may improve the physical properties of these composite BCP.

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Macro/Microporous Biphasic Calcium Phosphate Cylinders and Resorbable Collagen Membranes for Guided Bone Growth

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.439 ◽

2007 ◽

Vol 361-363 ◽

pp. 439-442

Author(s):

Borhane H. Fellah ◽

Said Kimakhe ◽

G. Daculsi ◽

Pierre Layrolle

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Bone Growth ◽

Bone Ingrowth ◽

Collagen Membrane ◽

Calcium Phosphate Ceramic ◽

Collagen Membranes ◽

Large Bone ◽

Empty Control ◽

Large Bone Defects

This study aims at evaluating bone growth in critical-sized femoral defects of rats filled with macro micro porous biphasic calcium phosphate ceramic (MBCP) cylinders surrounded or not by a resorbable collagen membrane. Femoral defects left empty (control) exhibited partial bone ingrowths after 3 and 6 weeks and were completely healed at 12 weeks. The defects filled with the collagen membranes appeared partially healed suggesting that the membranes constraint bone ingrowth. Bone formation occurred around the collagen membrane which partially degraded over time. In the MBCP/membrane group, bone has grown inside the macro pores of the MBCP cylinders. Bone ingrowth was more rapid and abundant in the defects filled with MBCP alone. The combination MBCP/collagen membrane may be beneficial for the reconstruction of large bone defects without using repetitive surgeries and autologous bone grafting.

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Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth

Biomaterials ◽

10.1016/s0142-9612(97)00180-4 ◽

1998 ◽

Vol 19 (1-3) ◽

pp. 133-139 ◽

Cited By ~ 452

Author(s):

Olivier Gauthier ◽

Jean-Michel Bouler ◽

Eric Aguado ◽

Paul Pilet ◽

Guy Daculsi

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

Calcium Phosphate Ceramics

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Calcium Phosphate Ceramic Blasting on Titanium Surface Improve Bone Ingrowth

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1351 ◽

2007 ◽

Vol 361-363 ◽

pp. 1351-1354 ◽

Cited By ~ 2

Author(s):

Eric Goyenvalle ◽

Eric Aguado ◽

Ronan Cognet ◽

Xavier Bourges ◽

G. Daculsi

Keyword(s):

Surface Roughness ◽

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Titanium Surface ◽

Bone Ingrowth ◽

High Surface ◽

Titanium Implants ◽

Calcium Phosphate Ceramic ◽

Abrasive Particles ◽

Roughened Surface

Surface roughness modulates the osseointegration of orthopaedic and dental titanium implants. High surface roughness is currently obtained by blasting of titanium implants with silica or aluminium abrasive particles. This process includes into the surface abrasive particles and may cause the release of cytotoxic silica or aluminium ions in the peri implant tissue. To overcome this drawback, we currently develop an innovative gridblasting process using Biphasic Calcium Phosphate (BCP) particles (RBBM Resorbable and Biocompatible Blast Media) to generate biocompatible roughened titanium surface. This work present the technique of blasting using RBBM particles to provide a roughened surface which does not release cytotoxic elements and (ii) to assess the effects of such a roughened surface for bone osteointegration in critical size rabbit defect. Our results demonstrate that resorbable biphasic calcium phosphate abrasive particles can be used to create titanium surface roughness. This grid blasting process increases surface roughness of titanium implants and offers a non cytotoxic surface for rapid and efficient osteointegration.

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Synchrotron X-ray microtomography (on a micron scale) provides three-dimensional imaging representation of bone ingrowth in calcium phosphate biomaterials

Biomaterials ◽

10.1016/s0142-9612(03)00335-1 ◽

2003 ◽

Vol 24 (25) ◽

pp. 4591-4601 ◽

Cited By ~ 112

Author(s):

P Weiss ◽

L Obadia ◽

D Magne ◽

X Bourges ◽

C Rau ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Ingrowth ◽

Three Dimensional ◽

Three Dimensional Imaging ◽

X Ray

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Integrated 3D Information for Custom-Made Bone Grafts: Focus on Biphasic Calcium Phosphate Bone Substitute Biomaterials

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17144931 ◽

2020 ◽

Vol 17 (14) ◽

pp. 4931

Author(s):

Alessandra Giuliani ◽

Maria Laura Gatto ◽

Luigi Gobbi ◽

Francesco Guido Mangano ◽

Carlo Mangano

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Alveolar Bone ◽

Sintering Temperature ◽

Calcium Phosphates ◽

Compressive Loading ◽

Peak Temperature ◽

X Ray Diffraction ◽

X Ray ◽

Custom Made

Purpose: Several studies showed that the sintering temperature of 1250 °C could affect the formation of α-Ca3(PO4)2, which is responsible for the reduction of the hardness value of biphasic calcium phosphate biocomposites, but they did not evaluate the inference of the sintering time at peak temperature on transition of β-Ca3(PO4)2 to α-Ca3(PO4)2. This analysis explored, in an innovative way, inferences and correlations between volumetric microstructure, mechanical properties, sintering temperature, and time at peak temperature in order to find the best sintering conditions for biphasic calcium phosphate composites grafted in severe alveolar bone defects. Methods: Sintered biphasic calcium phosphates (30%-hydroxyapatite/70%-tricalcium phosphate) were tested by microCT imaging for the 3D morphometric analysis, by compressive loading to find their mechanical parameters, and by X-ray diffraction to quantify the phases via Rietveld refinement for different sintering temperatures and times at the peak temperature. Data were analysed in terms of statistical inference using Pearson’s correlation coefficients. Results: All the studied scaffolds closely mimicked the alveolar organization of the jawbone, independently on the sintering temperatures and times; however, mechanical testing revealed that the group with peak temperature, which lasted for 2 hours at 1250 °C, showed the highest strength both at the ultimate point and at fracture point. Conclusion: The good mechanical performances of the group with peak temperature, which lasted for 2 hours at 1250 °C, is most likely due to the absence of the α-Ca3(PO4)2 phase, as revealed by X-ray diffraction. However, we detected its presence after sintering at the same peak temperature for longer times, showing the time-dependence, combined with the temperature-dependence, of the β-Ca3(PO4)2 to α-Ca3(PO4)2 transition.

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