scholarly journals Engineering of Skeletal Muscle Tissue Using Myoblast-Seeded Bovine Pericardium for Reconstruction of Abdominal Wall Defect in a Rabbit Model

2010 ◽  
Vol 8 ◽  
pp. 9-21
Author(s):  
T. Ayele ◽  
A.B.Z. Zuki ◽  
M.M. Noordin ◽  
B.M.A. Noorjahan
Keyword(s):  
Skeletal Muscle ◽  
Treatment Group ◽  
Abdominal Wall ◽  
Muscle Tissue ◽  
Abdominal Wall Defect ◽  
Rabbit Model ◽  
Bovine Pericardium ◽  
Skeletal Muscle Tissue ◽  
Control Group ◽  
Abdominal Wall Defects

A novel tissue engineered construct was used to engineer skeletal muscle tissue for reconstruction of abdominal wall defects, which is a common challenge to surgeons, due to insufficient autogenous tissue. Myoblasts were isolated from soleus muscle fibers, seeded onto the scaffold and cultivated in vitro for 5 days. Full-thickness abdominal wall defects (3 x 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups (n=9 each). The defects of the first group were repaired with myoblast seeded bovine pericardium (treatment group) whereas the second group involved non-seeded bovine pericardium (control group). Three animals were sacrificed at 7, 14, and 30 days post-implantation from each group and the explanted specimens were subjected to macroscopic, light, fluorescence and electron microscopic analysis. In each case, the tissue engineered construct was thicker from deposition of newly formed collagen with neo-vascularisation, than the control group. Most importantly, multinucleated myotubes and myofibers were only detected in the treatment group. Therefore, this study demonstrates that myoblast-seeded bovine pericardium construct can provide a structural replacement for severe and large abdominal wall defects with profound regeneration of skeletal muscle tissues.

scholarly journals Effect of Papaya Seed Extract (Carica papaya Linn.) on Glucose Transporter 4 (GLUT 4) Expression of Skeletal Muscle Tissue in Diabetic Mice Induced by High Fructose Diet

2017 ◽  
Vol 22 (2) ◽  
pp. 131
Author(s):  
Devyani Diah Wulansari ◽  
Achmad Basori ◽  
Suhartati Suhartati
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Seed Extract ◽  
Skeletal Muscle Tissue ◽  
Control Group ◽  
Test Results ◽  
High Fructose Diet ◽  
Glut4 Expression ◽  
High Fructose ◽  
Papaya Seed

Ethnobotany surveys show that papaya seeds are widely used as herbs for the management of some diseases such as abdominal discomfort, pain, malaria, diabetes, obesity, and infection. This research was conducted to analyze the effect of papaya seed extract on GLUT4 expression on skeletal muscle tissue of DM type II model induced by high fructose diet. This study used 24 animals, divided into 4 groups of negative control group, treated with papaya seed extract 100 mg / kgBB, 200 mg / kgBW and 300 mg / kgBW, was adapted for 14 days then induced by fructose solution 20% Orally with a dose of 1.86 grams / kgBB for 56 days. The treatment group was given papaya seed extract in accordance with the dose of each group for 14 days. GDP levels was measured using a spectrophotometer. Skeletal muscle tissue is used on the gastrocnemius part. GLUT4 expression was measured through a Immunoreactive Score (IRS) method with immunohistochemical staining using GLUT4 polyclonal antibodies. Comparative test results showed that there were significant differences between groups (p <0.05) in final GDP variables and GLUT4 expression. Pearson correlation test results show that the value p = 0.001, meaning there is a significant relationship between GLUT4 expression with final GDP levels. The result of simple linear regression analysis showed that p = 0,000 (<0,05), meaning that dose of papaya seed extract had a significant influence on GLUT4 expression.

scholarly journals Use of alloplastic meshes in abdominal wounds of rats with induced peritonitis

2014 ◽  
Vol 27 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Rafael Calvão BARBUTO ◽  
Ivana DUVAL-ARAUJO ◽  
Sumara Marques BARRAL ◽  
Raphael Grossi ROCHA ◽  
Cristiane de Souza BECHARA ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Abdominal Wall ◽  
Granulation Tissue ◽  
Abdominal Wall Defect ◽  
Test Group ◽  
Microscopic Analysis ◽  
Female Rats ◽  
Control Group ◽  
Abdominal Wall Defects ◽  
Wall Defect

Background : The use of alloplastic meshes has been historically contra-indicated in patients with infection. Aim : To evaluate the use of polypropylene meshes in the treatment of abdominal wall defects in rats with peritonitis. Methods : Twenty Wistar female rats were divided into two groups: induction of peritonitis (test group) and without peritonitis (control group). An abdominal wall defect was created in all animals, and polypropylene mesh was applied. The evaluation of the tensile strength of the mesh was carried out using tensiometer and microscopic analysis of the healing area was done. Results : More adhesion of the mesh to the rat abdominal wall was observed in test group. The histopathological analyses showed prevalence of moderate to accentuated granulation tissue in both groups, without significant differences. Conclusion : The use of the mesh coverage on abdominal wall defects of rats with induced peritonitis did not show worse results than its use in healthy animals, nor was its integration to the resident tissue any worse.

scholarly journals Investigation of a connection between abdominal wall defects and severity of the herniation in fetuses with gastroschisis and omphalocele

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natasha T. Logsdon ◽  
Carla M. Gallo ◽  
Luciano Alves Favorito ◽  
Francisco J. Sampaio
Keyword(s):  
Abdominal Wall ◽  
Iliac Crest ◽  
Abdominal Wall Defect ◽  
Control Group ◽  
Abdominal Wall Defects ◽  
Internal Organs ◽  
Wall Defect ◽  
Mean Values ◽  
Biometric Parameters ◽  
Post Test

AbstractAnalyze the biometric parameters and the size (area) of abdominal wall defect (AWD) in fetuses with gastroschisis and omphaloceles and correlate them with the herniated internal organs. We studied 22 fetuses (11 with AWDs and 11 without anomalies). In all fetuses we evaluated the xiphopubic distance (XPD) and iliac crest distance (ICD). In fetuses with AWDs we dissected the abdominal wall and measured the width and length of the defect for calculating its area and studying the correlation between the size of the defect with the organs that were herniated. For statistical analysis, the Anova and Tukey post-test were used (p < 0.05). The XPD in the control group had mean of 4.2 mm (2.3–5.9; SD ± 1.11), while in the AWDs it was 4.2 mm (2.9–5.5; SD ± 0.98) (p = 0.4366). The ICD had mean values of 2.5 mm (1.6–3.4; SD ± 0.58) in the control group, and 2.3 mm (1.2–3.0; SD ± 0.56) in AWDs fetuses (p = 0.6963). The number of herniate organs do not have significant correlation with the area of the defect (r2 = 0.2504, p = 0.5068). There is no correlation between the size (area) of abdominal wall defects and the number of the internal organs that herniated. Therefore, the hole size is not a predictor of the severity of the gastroschisis or omphalocele.

scholarly journals Comparative Cellular Local Response in Abdominal Defect Plastic Surgery with Bovine Pericardium and Bovine Fascia Preserved in Formaldehyde in Experimental Rabbits

2017 ◽  
Vol 23 (2) ◽  
pp. 83-93
Author(s):  
Eva Gudemac ◽  
S. Babuci ◽  
C. Tica ◽  
V. Petrovici ◽  
V. Nacu ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Direct Contact ◽  
Anterior Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Bovine Pericardium ◽  
Congenital Defects ◽  
Abdominal Wall Defects ◽  
Wall Defect ◽  
Fascia Graft ◽  
Subcutaneous Layer

AbstractIn the present paper, we refer to a method of primary closure of congenital defects of the anterior abdominal wall with tensionless viscero-abdominal disproportion. The study group included 10 animals subjected to surgery of the abdominal wall defect closure with bovine pericardium graft preserved in 0.5% formaldehyde, and 5 rabbits of the same breed and weight, in which bovine fascia graft was used as implant, being preserved in 0.5% formaldehyde. The abdominal anterior wall defect was made surgically by excision of the musculo-fascial structures and peritoneum. Bovine pericardium graft and bovine fascia graft were placed and fixed posteriorly to rectus abdominals muscles, having direct contact with the intra-abdominal contents and protected by suturing skin and subcutaneous layer.The purpose of the study was to perform a comparative postoperative evaluation of local macroscopic and microscopic changes that develop after reconstruction of the major abdominal wall defects experimentally induced in rabbits, using bovine pericardium and bovine fascia grafts preserved in 0.5% formaldehyde.In cases of major fascial defects of the anterior abdominal wall, bovine pericardium graft has acceptable strength and biocompatibility, having stabilizing properties of the abdominal wall due to the development of the connective tissue layer located between the implant and the subcutaneous layer. Bovine fascia grafts preserved in formaldehyde have an insignificant irritating and inflammatory action on the intestinal loops compared with bovine pericardium, and do not induce the development of a significant abdominal adhesion process, this allowing their use in the abdominal fascial defects closure with placement in direct contact with the abdominal contents.

scholarly journals Microvascularization is not a limiting factor for exercise in adults with cerebral palsy

2018 ◽  
Vol 125 (2) ◽  
pp. 536-544 ◽  
Author(s):  
Ida Torp Andersen ◽  
Adrian Harrison ◽  
Rikke Broholm ◽  
Anja Harder ◽  
Jens Bo Nielsen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cerebral Palsy ◽  
Muscle Tissue ◽  
Acute Exercise ◽  
Skeletal Muscle Tissue ◽  
Control Group ◽  
Limiting Factor ◽  
Healthy Individuals ◽  
Range Of Movement ◽  
Healthy Control

Muscle contractures are a common complication in patients with central nervous system (CNS) lesions which limit range of movement and cause joint deformities. Furthermore, it has previously been shown that muscles with contractures have a reduced number of capillaries, indicating decreased tissue vascularization. The aim of the present study was to investigate the microvascular volume (MV) at rest and after acute exercise in the muscle tissue of individuals with cerebral palsy (CP) and healthy control individuals. Contrast-enhanced ultrasound (CEUS) was used before and after 30 min of walking or running on a treadmill in 10 healthy control participants and 10 individuals with CP to detect MV of their skeletal muscle tissue. A significant increase in the MV was observed after exercise both in the adult CP group (21–53 yr) and in the control group (21–52 yr) (1.8 ± 0.8 ΔdB to 3.1 ± 0.9 ΔdB or 42.9% and 1.5 ± 0.6 ΔdB to 2.5 ± 0.9 ΔdB or 39.0%, respectively). Furthermore, a difference in the resting MV was observed between the most severe cases of CP [gross motor function classification scale (GMFCS) 3 and 4] (2.3 ± 0.5 ΔdB) and the less severe cases (GMFCS 1 and 2) (1.5 ± 0.2 ΔdB). When the CP group was walking (3.4 km/h), the lactate levels, Borg score, and heart rate matched the level of controls when they were running (9.8 km/h). In conclusion, individuals with CP become exhausted at much lower exercise intensities than healthy individuals. This is not explained by impaired microvascularization, since the MV of the individuals with CP respond normally to increased O2 demand during acute exercise. NEW & NOTEWORTHY Cerebral palsy (CP) patients were less physically active compared with typically developed individuals. This may affect the microvascularization. We observed that the CP group became exhausted at much lower exercise intensities compared with healthy individuals. However, impaired microvascularization was not the reason for the decreased physical activity as the CP group responded normally to increased O2 demand during acute exercise. These results indicate that walking may be recommended as an intervention to train and maintain skeletal muscle tissue in individuals with CP.

scholarly journals SKELETAL MUSCLE POSTTRAUMATIC REGENERATION RESPONSE TO HUMIC PELOID MEDICATION

2019 ◽  
Vol 16 (32) ◽  
pp. 96-107
Author(s):  
Galina N SUVOROVA ◽  
Natalia N VOLOGDINA ◽  
Nadezhda P AVVAKUMOV ◽  
Maria Y KRIVOPALOVA
Keyword(s):  
Skeletal Muscle ◽  
Humic Acids ◽  
Muscle Tissue ◽  
Muscle Fibers ◽  
Skeletal Muscle Tissue ◽  
Control Group ◽  
Acid Extraction ◽  
Zinc Ions ◽  
Posttraumatic Regeneration ◽  
Regeneration Response

One of the relevant tasks of current morphology is the investigation of tissue regenerative potential and research for new medications that improve recovery process efficiency. Nowadays, clinical specialists focus on medications that are based on natural compounds. However, such medication influence on the processes that occur within the injured tissues is still not identified. The purpose of this study was to investigate skeletal muscle tissue posttraumatic regeneration response to humic peloid medication, based on humic acids modified by Zinc ions. Humic acid extraction was carried out by means of patent procedure. The study included laboratory Wistar rats with hyperextension of front femur muscle. The preparations were studied by means of light and electronic microscopy and autoradiography. Histologic preparations evaluation showed that under peloid medication exposure the apolexis processes within muscle fibers rupture are inhibited, interstitial edema becomes restricted by the injured area, vessel growth into the area of damaged capillaries is stimulated, macrophages migrate and the area and duration of posttraumatic inflammation decrease. Additionally, peloid medication intake shortens the length of skeletal muscle tissue reparative histogenesis stages: myosatellitocytes are activated earlier than in the control group, myoblasts and myosymplasts are detected, the separation of nuclear sarcoplasmatic areas from partly injured muscle fibers is stimulated, myotubules appear 3 – 5 days earlier than in control group. Overall, muscle tissue regeneration efficiency increases by 21%. Obtained results allow us to conclude that peloid medication based on humic acids modified by Zinc ions positively influence the stimulation of the regeneration process. This will lead to further investigation of humic substances: fulvic, hymatomelanic, humin and humic acids of peloids as medications and their implementation in clinical practice.

scholarly journals Acellular dermal graft for repair of abdominal wall defects in rabbits

2006 ◽  
Vol 77 (2) ◽  
Author(s):  
A.K. Gangwar ◽  
A.K. Sharma ◽  
Naveen Kumar ◽  
N. Kumar ◽  
S.K. Maiti ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Test Group ◽  
Control Group ◽  
Abdominal Wall Defects ◽  
Full Thickness ◽  
Dermal Graft ◽  
Group I ◽  
Group A ◽  
Acellular Dermal

Sixteen clinically healthy New Zealand white rabbits of either sex were divided into 2 equal groups (I and II) of 8 animals each. Under thiopental sodium (2.5 %) anaesthesia a 2 × 3 cm full-thickness abdominal wall defect in the mid-ventral abdominal wall was created and repaired with an acellular dermal graft (ADG) in all the animals of group I (test group). In animals of group II (control group) a full-thickness linear midline abdominal muscular wall incision was made and repaired with a continuous suture pattern using 2-0 nylon.

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