Antibacterial Effects of Sol-Gel Derived Zinc-Containing Hydroxyapatite

2007 ◽  
Vol 361-363 ◽  
pp. 139-142 ◽  
Author(s):  
Xin Chang Shi ◽  
H.Z. Jiang ◽  
J. Xue ◽  
Yun Mao Liao ◽  
L.Y. Xiao ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Sol Gel ◽  
Brain Heart Infusion ◽  
Zinc Content ◽  
Atomic Emission ◽  
Biological Properties ◽  
Zinc Ions ◽  
Antibacterial Effects ◽  
Inductively Coupled

In order to modify the biological properties of pure hydroxyapatite (HAp), two kinds of zinc-containing HAp, which had zinc content of 5.10wt% and 2.54wt%, respectively, were synthesized via sol-gel technique. The obtained coatings were characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM). Then the antibacterial effects were evaluated through bacteriostatic test on Streptococcus mutans (S.mutans) in vitro and zinc ions releasing ability was investigated through detecting the zinc ions concentration by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). The obtained coatings were observed to possess typical apatite peaks in XRD patterns and high homogeneous and porous surfaces in SEM morphology. The coating layer demonstrated good releasing ability in Brain Heart Infusion (BHI) liquid and obviously inhibitory effects to the growth and proliferation of S. mutans. Based on the results obtained above, it is concluded that the sol-gel derived zinc-containing hydroxyapatite could be applied as an antibacterial effective biomaterial.

scholarly journals In vitro Antimicrobial Effects of Virus Block, Which Contains Multiple Polyoxometalate Compounds, and Hygienic Effects of Virus Block-Supplemented Moist Hand Towels

Pharmacology ◽  
2019 ◽  
Vol 104 (1-2) ◽  
pp. 98-112 ◽  
Author(s):  
Katsuaki Dan ◽  
Naohiro Katoh ◽  
Takaaki Matsuoka ◽  
Katsuyuki Fujinami
Keyword(s):  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Bacterial Flora ◽  
Atomic Emission ◽  
Time Dependent ◽  
Antimicrobial Effects ◽  
Inductively Coupled ◽  
Before And After ◽  
Normal Bacterial Flora

Background: Historical evidence has verified the multifaceted antiviral efficacy of polyoxometalates (PMs). Methods: We carried out a study to investigate the antimicrobial effects of each of the 5 substances comprising virus block (VB): 3 PMs that have antibacterial and antiviral activity, an antibiotic agent, and an antibacterial agent. We also investigated the effectiveness of the addition of VB to moist hand towels in a study involving 120 volunteers. The time-dependent changes in metal ion concentrations in aqueous VB solution were analyzed using inductively coupled plasma atomic emission spectroscopy. Results: The metal elements in the aqueous VB solution remained stable for 12 weeks without undergoing time-dependent changes. Discussion: Further investigations were performed to study hand hygiene using moist hand towels in daily life settings. To this end, 120 volunteers provided 240 specimens that were used to investigate the presence of antibacterial compounds on the volunteers’ hands before and after hand towel use. An aliquot of each specimen was suspended in phosphate-buffered saline and plated on agar media, and the number of colonies formed was counted. Normal bacterial flora found on the hands of the volunteers was investigated before and after the use of 4 different moist hand towels. Conclusions: The effects of VB and PMs were superior to those of commercial moist hand towels, indicating that effective data were obtained that may be useful for the practical application of the tested items.

The role of oxidative stress on the effect of 1,4,7,10,13,16-hexathiacyclooctadecane on copper and zinc toxicity in HepG2 cells

2003 ◽  
Vol 22 (2) ◽  
pp. 89-93 ◽  
Author(s):  
P W Smet ◽  
M Elskens ◽  
F Bolle ◽  
P J Dierickx
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Inductively Coupled Plasma ◽  
Combined Treatment ◽  
Zinc Content ◽  
Atomic Emission ◽  
Zinc Toxicity ◽  
Ligand Complex ◽  
Inductively Coupled ◽  
Copper And Zinc

Experiments have shown that 1,4,7,10,13,16-hexathiacy clooctadecane (L3) increased the Cu2 toxicity on HepG2 cells, whereas the combination Zn2 /L3 was less toxic relative to the metal control. In all cases, glutathione (GSH) levels were decreased and vitamins C and E supplementation partially counteracted the increased toxicity in the Cu2 /L3-treated cells. The previously observed effects of this hexathiamacrocyclic ligand (L3) on the Cu2 and Zn2 toxicity were further investigated by first depleting the intracellular GSH levels by means of L-buthionine S,R-sulphoximine. Combined treatment with Cu2 /L3 resulted in complete cell death, whereas for Zn2 /L3 no severe effects were observed. Direct measurement of reactive oxygen species (ROS) revealed that Cu2 induced a high degree of oxidative stress on the cells. This was not the case for Zn2. The results proved a previously proposed mechanism in which GSH is used to conjugate the metal–ligand complex, but as a result of this, GSH is no longer available for inactivation of ROS. Also, both the intracellular copper and zinc content were determined for each experiment by means of inductively coupled plasma–atomic emission spectroscopy. According to these data, zinc is depleted in Cu2 /L3-treated cells, which could have consequences on superoxide dismutase and as a result of this on the amount of oxidative stress.

scholarly journals Synthesis, characterization and catalytic activity of partially substituted La1−xBaxCoO3 (x ≥ 0.1 ≤ 0.4) nano catalysts for potential soot oxidation in diesel particulate filters in diesel engines

2020 ◽  
Vol 11 (1) ◽  
pp. 52-57
Author(s):  
Kayode Akinlolu ◽  
Bamgboye Omolara ◽  
Tripathi Shailendra ◽  
Akinsiku Abimbola ◽  
Ogunniran Kehinde
Keyword(s):  
Surface Area ◽  
Inductively Coupled Plasma ◽  
Research Study ◽  
Sol Gel ◽  
Soot Oxidation ◽  
Atomic Emission ◽  
Catalytic Performance ◽  
Inductively Coupled ◽  
Perovskite Catalysts ◽  
Nano Catalysts

AbstractThe sol gel method was used in preparing a series of A site partially substituted La1−xBaxCoO3 (x ≥ 0.1 ≤ 0.4) perovskite catalysts coded LBC1, 2, 3, and 4 and their potential as catalysts for soot oxidation were evaluated. The Brunauer–Emmett–Teller (BET), Inductively Coupled Plasma Atomic Emission Spectroscopy (ICPAES), Thermogravimetric/Differential Thermal Analysis (TGA/DTG), X-ray analysis (XRD) were used in characterizing the prepared perovskite catalyst. The result shows that at (x≥ 0.2 ≤ 0.4), there was an increase in surface area when we compare it with that of x = 0. The increase in surface area helps in increasing the catalytic performance of the catalyst. Also, when evaluating the catalytic performance of the synthesized catalysts, it was observed that doping the perovskite catalysts helped in the general improvement of the catalytic performance for soot oxidation. The best performance in this research study with a T50 of 484 °C was observed at x = 0.2 catalyst (LBC2). This shows that the non-noble perovskite catalysts prepared in this research study has the potential to replace the noble metal based catalysts used presently in the diesel automotive industry.

Synthesis and Characterization of Sol-Gel Derived Hydroxyapatite-Bioglass Composite Nanopowders for Biomedical Applications

2012 ◽  
Vol 12 ◽  
pp. 51-57 ◽  
Author(s):  
S. Adibnia ◽  
Ali Nemati ◽  
Mohammad Hosseien Fathi ◽  
S. Baghshahi
Keyword(s):  
Electron Microscopy ◽  
Inductively Coupled Plasma ◽  
Biomedical Applications ◽  
Sol Gel ◽  
In Vitro Study ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Inductively Coupled ◽  
Transmission Electron

The main purpose of this study is to prepare and characterize hydroxyapatite (HA)–10%wt bioglass (BG) composite nanopowders and its bioactivity. Composites of hydroxyapatite with synthesized bioglass are prepared at various temperatures. Suitable calcination temperature is chosen by evaluating of the phase composition. X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) techniques are utilized to characterize the prepared nanopowders. The bioactivity of the prepared composite samples is evaluated in an in vitro study by immersion of samples in simulated body fluid (SBF) for predicted time. Fourier transformed infrared (FTIR) spectroscopy and inductively coupled plasma (ICP) are used for evaluation of apatite formation and the bioactivity properties. Results show that HA-BG composite nanopowders are successfully prepared without any decomposition of hydroxyapatite. The suitable temperature for calcination is 600°C and the particle size of hydroxyapatite is about 40-70 nm. The apatite phase forms after 14 days immersing of the samples in SBF. It could be concluded that this process can be used to synthesize HA-BG composite nanopowders with improved bioactivity which is much needed for hard tissue repair and biomedical applications.

Ceramic Refractory Compositions with Biosolubility Characteristics

2015 ◽  
Vol 1765 ◽  
pp. 71-76
Author(s):  
Ma.G. Joaquín-Morales ◽  
G. Vargas-Gutiérrez ◽  
J.L. Rodríguez-Galicia ◽  
G.I. Vazquez-Carbajal ◽  
J. López-Cuevas
Keyword(s):  
Inductively Coupled Plasma ◽  
Atomic Emission ◽  
X Ray Diffraction ◽  
Plasma Atomic Emission ◽  
Powder Mixtures ◽  
X Ray ◽  
Inductively Coupled ◽  
Ceramic Refractory

ABSTRACTIn this work, we studied the dissolution of three different refractory compositions belonging to the ternary system SiO2-CaO-MgO into two Simulated Lung Fluids (SLF). The initial powder mixtures were uniaxially pressed and then sintered at 1300-1400 °C. The sintered samples were immersed for times from 1 to 21 days into a given SLF at 37 °C. The samples were characterized by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The dissolution of Ca2+, Mg2+ and Si4+ into the SLF was quantified by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). The in vitro studies suggested that all the considered materials had a potential to show a diminished biopersistence in vivo, due to reasons that depended on their chemical and phase composition.

Biocompatible luminescent nanoparticles on the basis of calcium phosphate

2011 ◽  
Vol 1355 ◽  
Author(s):  
Sofia Dembski ◽  
Moritz Milde ◽  
Emeline Dassonneville ◽  
Carsten Gellermann ◽  
Torsten Klockenbring ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Sol Gel ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Inductively Coupled ◽  
Transmission Electron ◽  
Core Shell Nanoparticles

ABSTRACTLuminescent lanthanide doped SiO2/Hydroxylapatite (HAp) core/shell nanoparticles (NPs) were synthesized by sol-gel technology. The resulting NPs exhibited an amorphous SiO2 core and a crystalline luminescent shell. The formation of the HAp layer was possible at pH 8.5. The characterization of the resulting NPs was done by transmission electron microscopy, X-ray diffraction analysis, inductively-coupled plasma combined with optical emission spectrometry, and photoluminescence spectroscopy. Additionally, the newly developed SiO2/HAp:Ln3+ core/shell NPs were tested for their biocompatibility, e. g. by an in vitro cell culture based assay.

Investigation of Strontium Effect on the Bioactive Behavior of Glasses in the System SiO2-CaO-P2O5-Na2O-SrO

2011 ◽  
Vol 493-494 ◽  
pp. 68-73 ◽  
Author(s):  
M. Erol ◽  
A. Özyuğuran ◽  
Ö. Özarpat ◽  
S. Küçükbayrak
Keyword(s):  
Inductively Coupled Plasma ◽  
Biological Properties ◽  
X Ray Diffraction ◽  
Bioactive Glasses ◽  
Strontium Content ◽  
X Ray ◽  
Inductively Coupled ◽  
Calcium Phosphate Layer ◽  
Plasma Techniques

In this study, it was aimed to produce bioactive glasses (SiO2-CaO-P2O5-Na2O-SrO) with the substitution of strontium in different weight percentages. Physical, thermal, and in vitro biological properties of the glasses were studied and compared to each other. In vitro simulated body fluid studies were performed to investigate the bioactivity of the produced glass samples. Scanning electron microscopy, X-ray diffraction, ultraviolet spectroscopy and inductively coupled plasma techniques were used to monitor changes in the glass surface and SBF composition. The results showed that all glasses favored precipitation of calcium phosphate layer when they were soaked in SBF; however bioactivity of the glasses increased with the increase of strontium content in the glasses.

Rapid Catalyst Capture Enables Metal-Free Parahydrogen-Based Hyperpolarized Contrast Agents

2018 ◽  
Author(s):  
Danila Barskiy ◽  
Lucia Ke ◽  
Xingyang Li ◽  
Vincent Stevenson ◽  
Nevin Widarman ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agents ◽  
Inductively Coupled Plasma ◽  
Organometallic Compounds ◽  
Atomic Emission ◽  
Platinum Group Metals ◽  
Resonance Spectroscopy ◽  
Plasma Atomic Emission ◽  
Inductively Coupled

<p>Hyperpolarization techniques based on the use of parahydrogen provide orders of magnitude signal enhancement for magnetic resonance spectroscopy and imaging. The main drawback limiting widespread applicability of parahydrogen-based techniques in biomedicine is the presence of organometallic compounds (the polarization transfer catalysts) in solution with hyperpolarized contrast agents. These catalysts are typically complexes of platinum-group metals and their administration in vivo should be avoided.</p> <p><br></p><p>Herein, we show how extraction of a hyperpolarized compound from an organic phase to an aqueous phase combined with a rapid (less than 10 seconds) Ir-based catalyst capture by metal scavenging agents can produce pure parahydrogen-based hyperpolarized contrast agents as demonstrated by high-resolution nuclear magnetic resonance (NMR) spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The presented methodology enables fast and efficient means of producing pure hyperpolarized aqueous solutions for biomedical and other uses.</p>

Rapid Catalyst Capture Enables Metal-Free Parahydrogen-Based Hyperpolarized Contrast Agents

2018 ◽  
Author(s):  
Danila Barskiy ◽  
Lucia Ke ◽  
Xingyang Li ◽  
Vincent Stevenson ◽  
Nevin Widarman ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agents ◽  
Inductively Coupled Plasma ◽  
Organometallic Compounds ◽  
Atomic Emission ◽  
Platinum Group Metals ◽  
Resonance Spectroscopy ◽  
Plasma Atomic Emission ◽  
Inductively Coupled

<p>Hyperpolarization techniques based on the use of parahydrogen provide orders of magnitude signal enhancement for magnetic resonance spectroscopy and imaging. The main drawback limiting widespread applicability of parahydrogen-based techniques in biomedicine is the presence of organometallic compounds (the polarization transfer catalysts) in solution with hyperpolarized contrast agents. These catalysts are typically complexes of platinum-group metals and their administration in vivo should be avoided.</p> <p><br></p><p>Herein, we show how extraction of a hyperpolarized compound from an organic phase to an aqueous phase combined with a rapid (less than 10 seconds) Ir-based catalyst capture by metal scavenging agents can produce pure parahydrogen-based hyperpolarized contrast agents as demonstrated by high-resolution nuclear magnetic resonance (NMR) spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The presented methodology enables fast and efficient means of producing pure hyperpolarized aqueous solutions for biomedical and other uses.</p>

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