Development of Thai Gac Fruit Extraction as a Multifunctional Cosmeceutical Ingredient for Antioxidant, Melanogenesis and Collagen Stimulating Activities

Gac fruit (Momordica cochinchinensis Spreng) is mostly found in southeast Asian countries. Previous studies have demonstrated that gac fruit is a major source of β-carotene, lycopene, and α-tocopherol. This study aimed to develop the extraction of Thai gac fruit as a potential active ingredient for antioxidant, melanogenesis, and collagen stimulating activities. In the present study, different parts of ripe Thai gac fruit, the peel, pulp, and seed membrane, were extracted using the accelerated solvent extraction (ASE) technique, an innovative plant extraction method. DPPH radical-scavenging activity, ABTS radical scavenging activity, and ferric reducing/antioxidant power (FRAP) assay were performed to compare the antioxidant activity derived from each part of the fruit. The effect of Thai gac fruit on melanogenesis and collagen stimulating activities was investigated using B16F1 and human fibroblast cell lines, respectively. The results demonstrate that the pulp (EC50 value of 7.42 ± 0.81 mg/mL) and the seed membrane (EC50 value of 76.43 ± 2.66 μg/mL) showed the strongest antioxidant activities in DPPH and ABTS assay, respectively. The peel clearly displayed the strongest antioxidant activity and significantly increased the FRAP value (247.67 ± 19.73 μM/mg). Furthermore, the peel showed the lowest melanin content (76.56 ± 7.13 % of control) in the B16F1 cell line, and clearly increased the most amount of collagen (21.87 ± 3.12%) in the human fibroblast cell line in comparison to the other fractions. Therefore, Thai gac fruit peel revealed the biological effects, including antioxidant, melanogenesis, and collagen stimulating activities. In conclusion, Thai gac fruit extracted using the ASE technique exhibited several effects that could be used as a potential active ingredient for cosmeceutical products, due to its antioxidant, melanogenesis, and collagen stimulating activities. Our findings provide potential uses of Thai gac fruit as a multifunctional cosmeceutical ingredient to be further explored in future studies.

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Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

Open Life Sciences ◽

10.2478/s11535-013-0247-0 ◽

2014 ◽

Vol 9 (2) ◽

pp. 149-155 ◽

Cited By ~ 4

Author(s):

Mirjana Marčetić ◽

Silvana Petrović ◽

Marina Milenković ◽

Marjan Niketić

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Chloroform Extract ◽

Volatile Fraction ◽

Scavenging Activity ◽

Antioxidant Power ◽

Aerial Parts ◽

Antimicrobial And Antioxidant Activity

AbstractThe chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

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HPLC Evaluation of Phenolic Profile, Nutritive Content, and Antioxidant Capacity of Extracts Obtained fromPunica granatumFruit Peel

Advances in Pharmacological Sciences ◽

10.1155/2013/296236 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 10

Author(s):

Sushil Kumar Middha ◽

Talambedu Usha ◽

Veena Pande

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Water Extract ◽

Scavenging Activity ◽

Fruit Peel ◽

Phenolic Profile ◽

Antioxidant Power

This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts ofPunica granatumpeel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 to 298.00 ± 24.86 mg GAE (gallic acid equivalents)/gm and 23.05 ± 1.54 to 49.8 ± 2.14 quercetin (QE) mg/g, respectively. The EC50of herbal extracts ranged from 100 µg/ml (0.38 quercetin equivalents), for AQPE, 168 µg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry.

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In vitro assessment of the protection from oxidative stress by various fractions of Artemisia incisa Pamp.

Journal of the Serbian Chemical Society ◽

10.2298/jsc110102116a ◽

2011 ◽

Vol 76 (10) ◽

pp. 1379-1386

Author(s):

A Aziz-Ur-Rehman ◽

Sehrish Gulzar ◽

Muhammad Abbasi ◽

Tayyaba Shahzadi ◽

Tauheeda Riaz ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Butylated Hydroxytoluene ◽

Total Phenolic ◽

Scavenging Activity ◽

Aqueous Fraction ◽

Antioxidant Power ◽

Ic50 Value

The methanolic extract of Artemisia incisa Pamp. was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods, i.e., the scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), the total antioxidant activity, the ferric reducing antioxidant power (FRAP) assay and the ferric thiocyanate assay. In addition, the total phenolics was determined. The obtained results revealed that among the studied fractions the ethyl acetate soluble fraction showed the most potent DPPH-radical scavenging activity with an IC50 value of 5.3 ? 0.71 ?g mL-1, which is even more effective than the standard antioxidant butylated hydroxytoluene (BHT) (IC50 value 0f 12.1? 0.92 ?g mL-1). The ethyl acetate fraction also showed the highest FRAP value (3677.13 ? 27.1 ?g TE mL-1), inhibition of lipid peroxidation (60.93 ? 0.84 % at 500 ?g mL-1) and total phenolic content (95.5 ? 0.05 ?g GAE g-1) as compared to other fractions. However, the remaining aqueous fraction was found to posses the highest antioxidant activity of all the fractions.

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Bagged Aronia Melanocarpa Tea: Phenolic Profileand Antioxidant Activity / Aronia Melanocarpa Filter Čaj: Fenolni Profil I Antioksidativna Aktivnost

Acta Facultatis Medicae Naissensis ◽

10.2478/afmnai-2014-0030 ◽

2014 ◽

Vol 31 (4) ◽

pp. 245-252 ◽

Cited By ~ 1

Author(s):

Jovana Veljković ◽

Jelena Brcanović ◽

Aleksandra Pavlović ◽

Snežana Mitić ◽

Biljana Kaličanin ◽

...

Keyword(s):

Antioxidant Activity ◽

Caffeic Acid ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Antioxidant Power ◽

Aronia Melanocarpa ◽

Tea Infusions

Summary While there is a large number of scientific papers reporting chemical composition and biological activities of Aronia melanocarpa, there is a lack information regarding the commercially available bagged tea. In order to supply new information on the antioxidant activity of the Aronia melanocarpa tea infusions, the aim of this study was to evaluate individual phenolic compounds which could be responsible for antioxidant activities of these beverages. Selected anthocyanins (cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin- 3-O-arabinoside, and cyanidin-3-O-xyloside), gallic acid, caffeic acid, rutin, morin, and protocatechuic acid were simultaneously detected from commercially available tea infusions using a High Performance Liquid Chromatographic (HPLC) method. The antioxidant activity was measured using five in vitro spectrophotometric methods: 1,1-diphenyl- 2-picrylhydrazyl radical scavenging activity (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid) radical cation scavenging activity (ABTS), ferric reducing-antioxidant power (FRAP) and reduction power (RP)Fe(III) to Fe(II). Obtained results showed that anthocyanins, predominantly of cyanidin-3-O-galactoside, are the major class of polyphenolic compounds in tea infusions. Among phenolic acids the most abundant is caffeic acid. A significant correlation between DPPH and ABTS and FRAP and RP suggested that antioxidant components in these beverages were capable scavenging free radicals and reducing oxidants. Generally, these beverages had relatively high antioxidant capacities and could be important dietary sources of antioxidant phenolics for the prevention of diseases caused by oxidative stress.

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Evaluation of Antidiabetic and Antioxidant Properties ofBrucea javanicaSeed

The Scientific World JOURNAL ◽

10.1155/2014/786130 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 19

Author(s):

Abdulwali Ablat ◽

Jamaludin Mohamad ◽

Khalijah Awang ◽

Jamil A. Shilpi ◽

Aditya Arya

Keyword(s):

Antioxidant Activity ◽

Inhibitory Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Oral Glucose ◽

Scavenging Activity ◽

Antioxidant Power ◽

Nitric Oxide Radical

The ethanol extract ofB. javanicaseed was fractionated with solvents of different polarities and tested for antioxidant activities by several assays including DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), ferrous ion chelating activity (FCA), and nitric oxide radical scavenging activity (NORSA) along with their polyphenolic contents. Antidiabetic activity was evaluated both in vitro and in vivo using a glycogen phosphorylaseα(GPα) inhibition assay and oral glucose tolerance test (OGTT) in nondiabetic rats. The ethyl acetate fraction (EAF), rich in tannin, exhibited the strongest antioxidant activities to DPPH, FRAP, and NORSA, except for FCA. The EAF also exerted a dose-depended inhibition of GPα(IC50= 0.75 mg/ml). Further evaluation of hypoglycemic effect on OGGT indicated that rats treated with EAF (125 mg/kg bw) showed a 39.91% decrease (P < 0.05) in blood glucose levels at 30 min, and continuous fall (P < 0.05) of 28.89% and 20.29% was observed in the following hours (60 and 90 min) compared to the normal control during OGTT. The EAF was applied to polyamide column chromatography, and the resulting tannin-free fraction was tested for both GPαinhibition and antioxidant (DPPH only) activity. The GPαinhibitory activity was retained, while antioxidant activity was lost (4.6-fold) after tannin removal. These results concluded that the GPαinhibitory activity initially detected was primarily due to the compounds other than tannins, whereas antioxidant activity was mainly due to the tannins.

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Investigation of Active Components and Antioxidant Activities in Peel and Pulp Extracts of Citrus Reticulata Blanco cv. Suavissima Fruit

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.1098 ◽

2011 ◽

Vol 343-344 ◽

pp. 1098-1102 ◽

Cited By ~ 1

Author(s):

Xiang Tao Chen ◽

Dong Qing Jiang ◽

Fang Fang Chen ◽

Ke Yuan

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Citrus Reticulata ◽

Scavenging Activity ◽

Frap Assay ◽

Antioxidant Power ◽

Citrus Reticulata Blanco

Citrus. reticulata Blanco cv. Suavissima friut has long been regarded as a food and medicinal plant. We investigated the antioxidant activity of the ethanol extract of peel and pulp part of fruit growned in Zhejiang Province, PR. China by several in-vitro systems of assay, namely DPPH radical-scavenging activity, ABTS.+ radical-scavenging activity, ferric reducing antioxidant power (FRAP) assay. Total phenolic content was measured by Folin-Ciocalteu reagent. The total flavonoids contents were measured using a modified colorimetric method.The extracts of different samples were found to have different levels of antioxidant activity in the systems tested. Ethanol extracts of the fruit showed stronger antioxidant activities compared with those of the commercial compound (Trolox). The results suggest that Citrus. reticulata Blanco cv. Suavissima friut can be a good source of natural antioxidant.

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Metabolomic Profiling of Antioxidant Compounds in Five Vachellia Species

Molecules ◽

10.3390/molecules26206214 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6214

Author(s):

Garland Kgosi More ◽

Stephen Meddows-Taylor ◽

Gerhard Prinsloo

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Proton Nuclear Magnetic Resonance ◽

Antioxidant Compounds ◽

Scavenging Activity ◽

Frap Assay ◽

Modeling Tools ◽

Antioxidant Power

The genus Vachellia, previously known as Acacia, belongs to the family Fabaceae, subfamily Leguminosae, which are flowering plants, commonly known as thorn trees. They are traditionally used medicinally in various countries including South Africa for the treatment of ailments such as fever, sore throat, Tuberculosis, convulsions and as sedatives. The aim of this study was to determine biochemical variations in five Vachellia species and correlate their metabolite profiles to antioxidant activity using a chemometric approach. The antioxidant activity of five Vachellia aqueous-methanolic extracts were analyzed using three methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) analysis and the ferric reducing antioxidant power (FRAP) assay by means of serial dilution and bioautography with the thin-layer chromatography (TLC) method. Amongst the Vachellia extracts tested, V. karroo, V. kosiensis and V. xanthophloea demonstrated the highest DPPH, ABTS+ and FRAP inhibitory activity. The antioxidant activities of DPPH were higher than those obtained by ABTS+, although these values varied among the Vachellia species. Proton nuclear magnetic resonance (1H NMR), coupled with multivariate statistical modeling tools such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), were performed to profile metabolites responsible for the observed activity. The OPLS-DA categorized the five Vachellia species, separating them into two groups, with V. karroo, V. kosiensis and V. xanthophloea demonstrating significantly higher radical scavenging activity than V. tortilis and V. sieberiana, which clustered together to form another group with lower radical scavenging activity. Annotation of metabolites was carried out using the ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-qTOF-MS), and it tentatively identified 23 metabolites of significance, including epigallocatechin (m/z = 305.0659), methyl gallate (m/z = 183.0294) and quercetin (m/z = 301.0358), amongst others. These results elucidated the metabolites that separated the Vachellia species from each other and demonstrated their possible free radical scavenging activities.

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Antioxidant Activities of Ethanol Extracts from Zanthoxylum avicennae

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.472.801 ◽

2014 ◽

Vol 472 ◽

pp. 801-804

Author(s):

Xi Feng Li ◽

Nan Nan Zheng ◽

Lu Tang ◽

Wei Xiao Zhang ◽

You Xu Wang ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Frap Assay ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Ethanol Extracts ◽

Dpph Radical Scavenging

The Zanthoxylum avicennae 95% EtoH extracts after concentration were dissolved in H2O and extracted with EtoAc and n-BuOH Successively. The n-BuOH layer was added to Diaion HP-20 macroporous resin column, then the resin was washed by distilled water to get rid of impurity, then washed by 20%, 40% and 60% methanol individually, and obtained M20, M40and M60respectively. And antioxidant activity was assessed using three methods: DPPH radical-scavenging activity, ABTS+radical-scavenging activity, ferric reducing antioxidant power (FRAP) assay. The results indicate that TEAC value of M40were higher than other part extracted from Zanthoxylum avicennae 95% EtoH extracts during three methods .The results of the experiments also can find M40is the strongest antioxidant activity part in Zanthoxylum avicennae 95% EtoH extracts and provide reference for further isolating the part M40.

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Phytochemical Contents and Antioxidant Activity of Medicinal Plants from the Rubiaceae Family in Thailand

Plant Science Today ◽

10.14719/pst.2021.8.1.882 ◽

2021 ◽

Vol 8 (1) ◽

pp. 24-31

Author(s):

Rungcharn Suksungworn ◽

Sutsawat Duangsrisai

Keyword(s):

Antioxidant Activity ◽

Plant Extracts ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Antioxidant Power ◽

Nitric Oxide Radical ◽

Promising Source ◽

Phytochemical Contents

Several plants of the Rubiaceae family possess potential pharmacological properties, such as antioxidant activity, for subsequent drug development. We investigated the methanolic extracts from the bark and wood of five Rubiaceae species for phenolic and flavonoid contents and antioxidant activity. Regarding the phytochemical contents and antioxidant activity, Mitragyna diversifolia wood (437.57 ± 9.90 mg GAE g-1) and Haldina cordifolia wood (30.11 ± 0.20 mg QE g-1) displayed the highest total phenolic content (TPC) and total flavonoid content (TFC) respectively. Morinda coreia bark followed the highest antioxidant activities (IC50 = 360.58 ± 19.28 µg ml-1) in the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), Catunaregam tomentosa bark (IC50 = 13.96 ± 5.32 µg ml-1) in the nitric oxide radical scavenging activity (NO), M. coreia wood (IC50 = 918.27 ± 0.16 µg ml-1) in the superoxide radical scavenging activity (SO) and M. coreia wood (IC50 = 236.65 ± 1.66 µg ml-1) in ferric reducing antioxidant power activity (FRAP). The TPC and TFC displayed strong correlations with DPPH in M. diversifolia wood and with FRAP in M. diversifolia bark and wood. We found high correlation between TFC and FRAP in all plant extracts except C. tomentosa wood, while no relation was detected between TFC and NO in all plant extracts. Comparing Rubiaceae species, the highest antioxidant potential were showed in C. tomentosa bark. Overall, it is worth mentioning that the Rubiaceae species exhibit potential as a promising source of natural antioxidants.

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ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15013 ◽

2017 ◽

Vol 9 (2) ◽

pp. 279 ◽

Cited By ~ 1

Author(s):

Ahmed R. Abu-raghif ◽

Ghaith A. Jasim ◽

Muneer M. Hanoon

Keyword(s):

Antioxidant Activity ◽

Cell Line ◽

Proliferative Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Extraction Yield ◽

Gas Chromatography Mass Spectrometry ◽

Scavenging Activity

Objective: The aim of this study was to investigate the capability of Zizyphus spina christi methanol extract to inhibit cancer cell line proliferation.Methods: The leaves of Zizyphus spina christi were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results: The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC50 was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC50 of 33.91 mg/ml.Conclusion: Methanol extract showed potential anti-proliferative activity against RD cell line, which could be due to its antioxidant activity.

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